检索结果-南通市图书馆

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： wei-hai yin~* neuroscience and neuroengineering research center,med-x research institute,shanghai jiao tong university, shanghai 200030,china

＜正＞Numerous studies have indicated oxidative stress as a key pathological factor in ischemic brain *** of the key links between oxidative stress and cell death is excessive activation of poly（ADP-ribose） polymerase-1 （PARP-1）,which plays an important role in the ischemic brain damage in male *** studies have also suggested that NAD+ depletion mediates PARP-1 cytotoxicity,and NAD+ administration can decrease ischemic brain injury.A number of recent studies have provided novel information regarding the mechanisms underlying the roles of oxidative stress and NAD+-dependent enzymes in ischemic brain *** particular interest,there have been exciting progresses regarding the mechanisms underlying the roles of NADPH oxidase and PARP-1 in cerebral *** examples,it has been suggested that androgen signaling and binding of PARP-1 onto estrogen receptors could account for the intriguing findings that PARP-1 plays remarkably differential roles in the ischemic brain damage of male and female animals;and some studies have suggested casein kinase 2,copper-zinc superoxide dismutase, and estrogen signaling can modulate the expression and activity of NADPH *** review summarizes these important current advances,and proposes future perspectives for the studies on the roles of oxidative stress and NAD+ in cerebral *** is increasingly likely that future studies on NAD- and NADP-dependent enzymes,such as NADPH oxidase,PARP-1,and sirtuins,would expose novel mechanisms underlying the roles of oxidative stress in cerebral ischemia,and suggest new therapeutic strategies for treating the debilitating disease.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Adjudin attenuates lipopolysaccharide-induced neuroinflammation

Adjudin attenuates lipopolysaccharide-induced neuroinflammat...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jia-xiang SHAO,Qian Reuben xIE,Ye-wei LIU,wei-Liang xIA~* neuroscience and neuroengineering center,School of Biomedical Engineering and med-x research institute, shanghai jiao tong university,shanghai 200030,china

＜正＞Neuroinflammation caused by microglial activation plays a key role in brain *** are activated in response to diverse stimuli and release multiple inflammatory *** seek for new drugs to modulate neuroinflammation,we recently found that Adjudin,a potential non-hormonal male contraceptive,exhibits additional function to reduce production of proinflammatory mediators in lipopolysaccharide（LPS）-stimulated microglial *** our present study,one-hour Adjudin pretreatment reduced IL-6 and IL-ip mRNA levels in BV2 microglial ***-6 secretion was also reduced with Adjudin *** determine whether Adjudin might have similar effects in vivo,mice were given Adjudin（100 mg/kg,i.p.） 3 h before and post LPS injection（2.5 mg/kg,i.p.）. The results showed that Adjudin decreased the induction of IL-6 and IL-1P mRNA by LPS in the ***,our data suggest that Adjudin might mitigate neuroinflammation.

关键词： Adjudin neuroinflammation IL-6 IL-1β BV2 microglial cells lipopolysaccharide

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Adeno-associated virus-mediated overexpression of netrin-1 attenuates ischemic brain injury in mice

Adeno-associated virus-mediated overexpression of netrin-1 a...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yong-Ting WANG~1,hai-Yan LU~1,Jian-Rong LIU~2,Li-Li ZENG~2,Guo-Yuan YANG~(1,2,*) 1 neuroscience and neuroengineering research center,med-x research institute,shanghai jiao tong university, shanghai 200030,china 2 Department of Neurology,Ruijin Hospital,School of medicine,shanghai jiao tong university,shanghai 200025,china

Objective Netrin-1 is one of the axon-guiding molecules that are critical for neuronal development. Netrin-1 was found to stimulate the proliferation and migration of human cerebral endothelial cells in vitro and also promote focal neovascularization in normal adult mouse brain in *** the present study,we investigate the role of overexpression of netrin-1 via adeno-associated virus(AAV) mediated-gene transfer in a transient middle cerebral artery occlusion(tMCAO) model of *** We constructed adeno-associated virus carrying Netrin-1(AAVNT -1) or GFP gene(AAV-GFP,as a control).2.5μL of AAV-NT-1 suspension with 2×10 virus particles was injected stereotactically into the lateral ventricle of male CD-1 mice at a rate of 0.2μL/*** amount of AAV-GFP or saline was injected as controls(n=21 per group).Seven days after AAV-NT-1 injection,these mice underwent 60 min of tMCAO followed by 7 to 28 d of ***-1 expression was quantified by Western blot and Netrin-1 localization was determined by *** infarct volume and animal behavioral outcomes(beam walk test and Rotor-Rod test) were examined until the end of ***-stained microvessels were counted to determine the effect of Netrin-1 on angiogenesis after *** Endogenous Netrin-1 expression was induced in the ischemic penumbra of mouse brain by transient *** expression of Netrin-1 in the ischemic hemisphere was further enhanced after AAV-NT-1 gene *** neurons and astrocytes but not endothelial cells expressed Netrin-1 following AAV-NT-1 gene *** of Netrin-1 promoted the recovery of focal cerebra blood flow after MCAO in the AAV-NT-1-transduced mice compared to control group(P < 0.05).Brain infarct volume was decreased and neurobehavioral outcomes were greatly improved in the AAV-NT-1 transduced mice compared to the AAV-GFP or saline injected group of mice up to 28 days after tMCAO(P < 0.05).Microvessel counts were significantly inc

关键词： adeno-asociated virus angiogenesis ischemia netrin-1 transient middle cerebral artery occlusion

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Netrin-1 hyper-expression of in the brain promotes angiogenesis and improves neurological outcome in transient MCAO mice

Netrin-1 hyper-expression of in the brain promotes angiogene...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： hai-Yan LU~1,Yong-Ting WANG~2,xiao-Song HE~2,Fa-Lei YUAN~2,xiao-Jie LING~2,Bo-Hua xIE~2,Guang-Hui TANG~2, Jun HUANG~2,Yao-Hui TANG~2,Sheng-Di CHEN~1,Guo-Yuan YANG~(1,2,*) 1 Departments of Neurology,Ruijin Hospital,shanghai jiaotong university School of medicine,shanghai 200025, china 2 neuroscience and neuroengineering research center,med-x research institute,shanghai jiao tong university, shanghai 200030,china

＜正＞Objective Netrin-1（NT-1） is one of the axon-guiding molecules that are critical for neuronal ***-1 stimulates proliferation and migration of human cerebral endothelial cells in vitro and promotes focal neovascularization in adult brain in *** is unknown whether NT-1 improves long-term functional outcome after ischemic *** aim of this study is to determine whether hyper-expression of NT-1 through adenoassociated virus（AAV） -mediated gene transfer enhances neurovascular remodeling and improves functional outcome in mouse model of focal cerebral *** AAV-NT-1 virus was stereotactic injected into the basal ganglia of male CD-1 mice（n=30）.Seven days after injection,these mice underwent 60 minutes of transient middle cerebral artery occlusion（tMCAO） followed by 1 to 4 weeks of *** of NT-1 and its receptors was quantified by Western blot and *** volume and behavioral analyses were performed after tMCAO. Synchronic radiation angiography was used to detect the changes of cerebral *** NT-1 expression in the ischemic hemisphere was enhanced after AAV-NT-1 gene *** neurons and astrocytes but not endothelial cells expressed ***-1 receptors（DCC and UNC5H2） also expressed in neurons and ***-expression of NT-1 decreased infarct volume in the AAV-NT-1-transduced mice compared to the control（P＜0.01）.Long-term observation demonstrated that neurobehavioral outcomes were greatly improved in the AAV-NT-1-tranduced mice compared to the control（P＜0.05）.Further study demonstrated that the microvessel counts were significantly increased in the AAV-NT-1 transduced mice（P＜0.05）.Synchrotron radiation angiography showed that the brunches of MCA was increased in NT-1 treated *** Our data demonstrate that NT-1 hyper-expression reduced ischemic injury and promoted functional *** may due to enhancement of focal neovascularization.

关键词： adeno-associated virus angiogenesis gene transfer ischemia middle cerebral artery occlusion netrin-1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

脑源成像技术及其应用研究进展

引用

中国科学：技术科学 2024年第2期54卷 196-224页

作者：魏玉烜王旭江海腾孟建军上海交通大学机械与动力工程学院机器人研究所上海200240 浙江大学医学院附属精神卫生中心(杭州市第七人民医院) 杭州310013

脑电图(EEG)和脑磁图(MEG)为观察和分析人脑活动提供了重要的无创测量工具. EEG/MEG可采集大规模神经集群放电活动引起的体表电势/磁场,采集的信号具备高时间分辨率,因而在神经科学、临床医学与神经工程等领域得到广泛的应用.然而, EEG/... 详细信息

脑电图(EEG)和脑磁图(MEG)为观察和分析人脑活动提供了重要的无创测量工具. EEG/MEG可采集大规模神经集群放电活动引起的体表电势/磁场,采集的信号具备高时间分辨率,因而在神经科学、临床医学与神经工程等领域得到广泛的应用.然而, EEG/MEG测得的是颅内神经集群活动经过脑组织、脑脊液、颅骨和头皮等人体容积导体组织弥散传播后引起的颅外电势/磁场,其空间分辨率低,无法反映神经活动的真实空间分布.源成像是一种结合人体头部组织的数学模型,用EEG/MEG来推导颅内神经活动源的成像技术,是目前公认的提高无创测量空间分辨率的有效技术.典型的源成像问题包括正问题建模和逆问题求解两个方面,其中逆问题求解是源成像的核心,而正问题建模是逆问题求解的基础,两者相辅相成.本文通过文献检索梳理和归纳目前存在的正问题建模和逆问题求解的典型方法和常用软件,介绍影响源成像精度的因素和评价源成像性能的指标和方法.另外,本文还着重介绍了源成像技术在神经科学、临床医学以及神经工程-脑机接口方面的应用和研究进展.最后,讨论了当前源成像技术面临的挑战,以及针对这些挑战该领域内的研究前沿和趋势,展望了源成像在推动神经科学、临床医学等科学研究和技术进步方面的潜力.

关键词：源成像源定位源重建脑电图脑磁图逆问题

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MicroRNA expression signature in human brain arteriovenous malfor-mations and brain hemangioblastoma

MicroRNA expression signature in human brain arteriovenous m...

引用

中国神经科学学会第十届全国学术会议

作者： Jun HUANG wei ZHU Qinzhu AN Yang WANG Song ZHANG Guanghui TANG Tony xU Yaning LI Yanqun LIU Yongting WANG Guo-Yuan YANG neuroscience and neuroengineering research center Med-X Research InstituteandSchool of Biomedical EngineeringShanghai Jiao Tong University Department of Neurology HuaShanHospitalFudanUniversity Department of Neurology Ruijin HospitalSchool of MedicineShanghai Jiao Tong University

Objectives:Small noncoding microRNAs（miRNAs） play critical roles in many biological and pathological processes and are differentially expressed in normal tissue and diseased *** arteriovenous malformations（BAVM） and brain hemangioblastoma are cerebrovascular diseases involving ***,the function of miRNAs in regulating blood vessel development of these two diseases is *** this study,we aim to investigate the miRNAs signature in human BAVM and human brain ***:Total RNA from the surgical specimen of BAVM patients（n =6）,hemangioblastoma patients（n =5）,and healthy individuals（n =5） were assessed using ExIQON Kit arrays（microRNA Ready-to-Use PCR）.739 miRNAs were validated with qRT-PCR analysis in order to establish the miRNA expression *** software was used to analyze all the preliminary qRT-PCR *** Pathway Analysis software was used to predict potential miRNA targets and to analyze their ***:miRNA arrays revealed that the expression levels of 7 and 80 miRNAs were significantly different（P＜0.05and passed Bonferroni correction） in BAVM patients and in brain hemangioblastoma patients compared to healthy controls,respectively.23 miRNAs expressed significantly differently（P＜0.05 and passed Bonferroni correction） in the BAVM patients compared to brain hemangioblastoma patients;of these significantly changed miRNAs,miR-137,miR-138,miR-431,miR-379 were down-regulated in both BAVM and hemangioblastoma patients compared to healthy controls while miR-451 was the only miRNA that is up-regulated in both patient ***-483-3p was greatly reduced in BAVM compared to brain hemangioblastoma patients（Fold change=101.98,P＜0.001）.Bioinformatic analysis indicated that miR-137,miR-138,miR-431,miR-379 and miR-451 were the potential miRNA targets that could be involved in ***:This study shows that there is a distinct miRNA expression signature in brain vascular *** fi

关键词： microRNA brain arteriovenous malformations brain hemangioblastoma

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：