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Root-fed Salicylic Acid in Grape Involves the Response Caused by Aboveground High Temperature

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Journal of Integrative Plant Biology 2008年第6期50卷 761-767页

作者： Hong-Tao Liu Yue-Ping Liu wei-dong huang College of Food Science &Nutntional Engineenng China Agncultural University Beijing 100083 China Department of Biotechnology Beijing Agricultural College Beijing 102206 China

In order to investigate the transportation and distribution of salicylic acid （SA） from root to aboveground tissues in response to high temperature, the roots of grape plant were fed with 14C-SA before high temperature treatment. Radioactivity results showed that progressive increase in SA transportation from root to aboveground as compared with the control varied exactly with the heat treatment time. Radioactivity results of leaves at different stem heights indicated that the increase in SA amount at the top and middle leaves during the early period was most significant in comparison with the bottom leaves. The up-transportation of SA from root to aboveground tissues was dependent on xylem rather than phloem. Autoradiographs of whole grape plants strongly approved the conclusions drawn above. Root-derived SA was believed to be a fundamental source in response to aboveground high temperature.

关键词： aboveground grape high temperature root salicylic acid

Clinical features and treatment in patients with acute 2,4-dinitrophenol poisoning

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第3期12卷 189-192页

作者： Yuan-qiang LU Jiu-kun JIANG wei-dong huang Department of Emergency the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective:To report clinical features and treatment of 16 cases of acute 2,4-dinitrophenol poisoning. Methods:A total of 16 patients suffering from acute poisoning due to non-oral exposure to 2,4-dinitrophenol were sent to our *** died within 3 h after admission,while the other 14 responded to supportive treatment and *** features and treatment of the patients were retrospectively analyzed and ***:Fourteen patients recovered and were discharged after four to six weeks of *** obvious poisoning sequelae were found in the three-month ***:Non-oral exposure to 2,4-dinitrophenol is *** and glucocorticoid treatments may be efficient measures to prevent mortality,but this requires further study.

关键词： 2 4-Dinitrophenol Poisoning Therapeutics Hemoperfusion Glucocorticoid

Case Report:Wernicke encephalopathy following splenectomy in a patient with liver cirrhosis:a case report and review of the literature

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 433-436页

作者： Xia-ping ZHANG Yuan-qiang LU wei-dong huang Department of Emergency the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China

Objective:To report a case of Wernicke encephalopathy in the early stage after ***:A nonalcoholic female patient with hepatitis B-related cirrhosis and hypersplenism underwent splenectomy in a local *** surgical complications occurred and the patient recovered ***,on the eighth postoperative day she developed psychiatric and neurological disturbance without an obvious *** was then admitted to our *** magnetic resonance imaging(MRI) with FLAIR T2 showed symmetric high-signal intensities in the periaqueductal area of the midbrain,which were consistent with Wernicke *** was thus given in-tramuscular thiamine ***:After the administration of thiamine,the patient's confused mental state resolved within 3 d,and her dystaxia gradually improved over the next 5 *** brain MRI with FLAIR T2 was re-examined one month after the episode,and showed nearly complete resolution of the previously abnormal signal intensities in the periaqueductal area of the ***:Physicians should be aware of the possibility of acute Wernicke encephalopathy,especially in patients with liver dysfunction.

关键词： Wernicke encephalopathy Liver cirrhosis Thiamine Splenectomy

siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells

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World Journal of Gastroenterology 2013年第44期19卷 8108-8113页

作者： dong Zhou Jie Yang wei-dong huang Jun Wang Qiang Zhang Department of General Surgery Xiangyang Hospital Affiliated to Hubei University of Medicine

AIM:To determine the effects of RNAi-mediated inhibition of the growth hormone receptor(GHR)gene on tumors and colon cancer cells in ***:Construction of a eukaryotic vector for human GHR expression,the pcDNA 6.2-GW/EmGFPsmall interfering RNAs(siRNAs)-GHR plasmid,was used to inhibit GHR ***-six BALB/c nude mice were randomly divided into groups and treated with normal saline(NS),recombinant plasmid(G2),growth hormone(GH),5-fluorouracil(FU),G2+FU or G2+FU+*** nude mouse was subcutaneously inoculated with 1×107human colon cancer SW480 cells;the nude mice were weighed before inoculation and on the 2nd,5th,8th,11th,14thand 17thday after *** nude mice were sacrificed after 17 *** subcutaneous tumor was removed and *** volume was measured on the 5th,8th,11th,14thand 17thday after *** expression of GHR protein in the tumor tissue was detected by Western blotting analysis,and the differences in GHR mRNA expression in the tumor tissue were detected by real-time quantitative reverse transcription-polymerase chain ***:Compared to the control group,the weights of the inoculated nude mice on the 17thday after inoculation were:G2:21.60±0.71 g,GH:21.64±0.45 g,FU:18.94±0.47 g,FU+G2:19.40±0.60 g,G2+FU+GH:21.04±0.78 g vs NS:20.68±0.66 g,P<0.05;the tumor volumes after the subcutaneous inoculation were:G2:9.71±3.82 mm3,FU:11.54±2.42mm3,FU+G2:11.42±1.11 mm3,G2+FU+GH:10.47±1.02 mm3vs NS:116.81±10.61 mm3,P<*** to the GH group,the tumor volumes were significantly decreased in the experimental *** GHR protein expression(G2:0.39±0.02,FU:0.40±0.02,FU+G2:0.38±0.01,G2+FU+GH:0.39±0.01 vs NS:0.94±0.02,P<0.05)and the GHR mRNA expression(G2:14.12±0.10,FU:15.15±0.44,FU+G2:16.46±0.27,G2+FU+GH:15.37±0.57 vs NS:12.63±0.14,P<0.05)were significantly decreased and increased,respectively,in the experimental ***:Inhibition of GHR in human colon cancer SW480 cells resulted in anti-tumor effects in nude

关键词： Growth hormone receptor Small interfering RNAs Colon cancer Gene therapy Signaling pathway

Assessment of the effects of induced anisometropia on binocularity with glasses-free 3D technique

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International Journal of Ophthalmology(English edition) 2023年第4期16卷 601-607页

作者： Xu-Bo Yang wei-dong huang Yong-Chuan Liao Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Optometry and Visual Science West China School of MedicineSichuan UniversityChengdu 610041Sichuan ProvinceChina

AIM:To assess the effect of experimentally induced anisometropia on binocularity in normal adults with glassesfree three-dimensional(3D)***:Totally 54 healthy medical students with normal binocularity in the cross-sectional study were *** was induced by placing trail lenses over the right eye,in 0.5 D steps including lenses of-0.5,-1,-1.5,-2,-2.5 D(hyperopic anisometropia)and lenses of+0.5,+1,+1.5,+2,+2.5 D(myopic anisometropia).The glasses-free 3D technique was used to evaluated not only fine stereopsis,but also coarse stereopsis,dynamic stereopsis,foveal suppression,and peripheral suppression in these ***-way analysis of variance was used to compare quantitative data such as fine stereopsis,coarse ***’s Chi-square test was performed to compare categorical data such as dynamic stereopsis,foveal suppression and peripheral ***:The subjects showed a statistically significant decline in fine stereopsis,coarse stereopsis,and dynamic stereopsis with increasing levels of anisometropia(P<0.001).Binocularity was af fected when induced anisometropia was more than 1 D(P<0.05).Foveal suppression and peripheral suppression were evident and increased in proportion to anisometropia(P<0.001).CONCLUSION:The relatively low degrees of anisometropia may have a potentially significant effect on high-grade binocular *** mechanisms underlying the defect of binocularity seem to involve not only foveal suppression,but also peripheral suppression.

关键词： anisometropia binocularity stereopsis glasses-free 3D technique

Effects of Temperature Acclimation Pretreatment on the Ultrastructure of Mesophyll Cells in Young Grape Plants （Vitis vinifera L. cv. Jingxiu） Under Cross-Temperature Stresses

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Journal of Integrative Plant Biology 2005年第8期47卷 959-970页

作者： Jun-Huan ZHANG wei-dong huang Yue-Ping LIU Qiu-Hong PAN College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

Leaves from annual young grape plants （Vitis vinifera L. cv. Jingxiu） were used as experimental materials. The ultrastructural characteristics of mesophyll cells in chilling-treated plants after heat acclimation （HA） and in heat-treated plants after cold acclimation （CA） were observed and compared using transmission electron microscopy. The results showed that slight injury appeared in the ultrastructure of mesophyll cells after either HA （38℃ for 10 h） or CA （8℃ for 2.5 d）, but the tolerance to subsequent extreme temperature stress was remarkably improved by HA or CA pretreatment. The increases in membrane permeability and malondialdehyde concentration under chilling （0℃） or heat （45℃） stress were markedly inhibited by HA or CA pretreatment. The mesophyll cells of plants not pretreated with HA were markedly damaged following chilling stress. The chloroplasts appeared irregular in shape, the arrangement of the stroma lamellae was disordered, and no starch granules were present. The cristae of the mitochondria were disrupted and became empty. The nucleus became irregular in shape and the nuclear membrane was digested. In contrast, the mesophyll cells of HA-pretreated plants maintained an intact ultrastructure under chilling stress. The mesophyll cells of control plants were also severely damaged under heat stress. The chloroplast became round in shape, the stroma lamellae became swollen, and the contents of vacuoles formed clumps. In the case of mitochondria of control plants subjected to heat stress, the outer envelope was digested and the cristae were disrupted and became many small vesicles. Compared with cellular organelles in control plants, those in CA plant cells always maintained an integrated state during whole heat stress, except for the chloroplasts, which became round in shape after 10 h heat stress. From these data, we suggest that the stability of mesophyll cells under chilling stress can be increased by HA pretreatment. Similarly, CA pretreatment can p

关键词： chilling stress cold acclimation cross-adaptation grape (Vitis vinifera L. cv. Jingxiu) plants heat acclimation heat stress ultrastructure.

Microstructure evolution and liquid phase separation in Ta-O hypermonotectic melts during laser-cladding

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China Foundry 2018年第3期15卷 222-227页

作者： Hai-ou Yang Meng Wang Xin Lin wei-dong huang State Key Laboratory of Solidification Processing Northwestern Polvtechnical University Xi'an Shaanxi 710072 PR China MHT Key Laboratory of Metal High Performance Additive Manufacturing and Innovative Design Northwestern Polytechnical University Xi'an Shaanxi 710072 PR China

A three-layer Ta_2O_5-containing coating was successfully fabricated by laser cladding on a pure Ta substrate. The maximum thickness of such a coating is about 1.6 mm. The microstructure, phase constitution and elemental distribution in the coating were investigated. Results show that the coating has been metallurgically bonded to the Ta substrate and the microstructure exhibits a graded change along the deposition direction from Ta substrate to the top of coating. In the layers I and II of the graded coating, the microstructure evolution can be confi rmed as a result of hypomonotectic reaction, but in the layer Ⅲ it was formed by hypermonotectic reaction. At the top of coating, the microstructure was still homogeneous although liquid phase separation had occurred, which can be attributed to the fact that the O-rich droplets do not have enough time to fl oat at high cooling rate. The theoretical calculation results show that during laser cladding, the solidifi cation time of the melt pool was less than 0.1 s, which fi ts well with the results from the experiment.

关键词： Laser cladding liquid phase separation microstructure evolution homogeneous structure

Clinical classification of chronic prostatitis:a preliminary investigation

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Asian Journal of Andrology 2000年第4期2卷 311-313页

作者： wei-dong huang Pei LIU Wen-Jie huang Xinjiang Jiayin Andrology Hospital,Urumuqi 830006,China Xinjiang Jiayin Andrology Hospital Urumuqi 830006 China. wdhuang@***

Aim: To propose a practical clinical classification for the chronic prostatitis (CP). Methods: The clinical fea-tures and the findings in the expressed prostatic secretion (EPS) in 804 cases of CP patients were retrospectively ana-lyzed. Results: Four types of CP were identified based on the clinical manifestations and the amounts of whiteblood cells (WBC) and lecithin in EPS. They were the latent type (85 cases; 10.6%), the common type (423 cases;52.6%), the persisting type (104 cases; 12.9%), and the active type (192 cases, 23.9%). The therapeutic efficacyfor these 4 subtypes were 40.4%, 76.8%, 30.8% and 37%, respectively; a statistical difference was noticed be-tween the common type and the persisting type (P < 0.01 ). Conclusion: The method of classification proposed bythe authors may help clinicians in the diagnosis and predicting the prognosis of CP. (Asian J Androl 2000 Dec; 2:311-313)

关键词： chronic bacterial prostatitis clinical classification curative efficacy

Jasmonic Acid is Induced in a Biphasic Manner in Response of Pea Seedlings to Wounding

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Journal of Integrative Plant Biology 2009年第6期51卷 562-573页

作者： Hao-Ru Yang Ke Tang Hong-Tao Liu Qiu-Hong Pan wei-dong huang College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China Institute of Geographic Sciences and Natural Resources Research the Chinese Academy of Sciences Beijing 100101 China

The role of jasmonic acid （JA） in plant wounding response has been demonstrated. However, the source of JA in wound signaling remains unclear. In the present study, pea seedlings were used as material to investigate the systemic induction of JA and the activation of lipoxygenase （LOX）-dependent octadecanoid pathway upon wounding. The results showed that endogenous JA could induce two peaks in the wounded leaves and the stalks, while only one peak in the systemic leaves. LOX activity and its protein amount were also induced and the stimulation mainly occurred in the late phase, while one peak of induction was present after pretreatment with JA. Applied nordihydroguaiaretic acid （NDGA）, an inhibitor of LOX activity, only inhibited the induction of JA in the late phase, and the resistance of pea was impaired. Furthermore, 13（S）- hydroperoxy-9（Z）, 11 （E）-octadecadienoic acid （13（S）-H（P）ODE） was confirmed to be the main product of LOX throughout the experimental time. in addition, immunocytochemical analysis also revealed the occurrence of JA biosynthesis and transport upon wounding. These results demonstrated that wound-induced JA in wounded leaves resulted from its biosynthesis and conversion from its conjugates, while in systemic leaves resulted from its transport and biosynthesis; and proved that the LOX pathway was vital to the wound-induced defense response involved in JA biosynthesis.

关键词： defense response jasmonic acid lipoxygenase pea (Pisum sativum L.).