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Jetting-based bioprinting:process,dispense physics,and applications

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Bio-Design and Manufacturing 2024年第5期7卷 771-799页

作者： wei Long ng Viktor Shkolnikov Singapore Centre for 3D Printing(SC3DP) School of Mechanical and Aerospace EngineeringNanyang Technological University(NTU)Singapore 639798Singapore HP Inc. Palo AltoCA 94304USA

Jetting-based bioprinting facilitates contactless drop-on-demand deposition of subnanoliter droplets at well-defined positions to control the spatial arrangement of cells,growth factors,drugs,and biomaterials in a highly automated layer-by-layer fabrication *** to its immense versatility,jetting-based bioprinting has been used for various applications,including tissue engineering and regenerative medicine,wound healing,and drug development.A lack of in-depth understanding exists in the processes that occur during jetting-based *** review paper will comprehensively discuss the physical considerations for bioinks and printing conditions used in jetting-based *** first present an overview of different jetting-based bioprinting techniques such as inkjet bioprinting,laser-induced forward transfer bioprinting,electrohydrodynamic jet bioprinting,acoustic bioprinting and microvalve ***,we provide an in-depth discussion of various considerations for bioink formulation relating to cell deposition,print chamber design,droplet formation and droplet ***,we highlight recent accomplishments in jetting-based *** present the advantages and challenges of each method,discuss considerations relating to cell viability and protein stability,and conclude by providing insights into future directions of jetting-based bioprinting.

关键词： 3D bioprinting Biofabrication Jetting-based Dispense physics Machine learning

Progress in Understanding the Functional Roles of Extracellular Vesicles in Reproduction

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Biomedical and Environmental Sciences 2020年第7期33卷 518-527页

作者： HAN ting ting LI wei LI Guo Ping The Key Laboratory of Geriatrics Beiji ng In stitute of GeriatricsBeijing HospitalNational Center of GerontologyNational Health CommissionInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Exosomes,a type of extracellular vesicles(EVs),were first discovered in sheep reticulocytes in 1983[1],and after four years,the name‘exosome’was officially given to them.A variety of cells secrete exosomes under both normal and pathological *** are mainly products of themultivesicular body formed by intracellular lysosomal invagi nation,and released into the extracellular space by fusion of the outer membrane of the multivesicular body with the cell membrane.

关键词： Exosomes cytes outer

ON ALGORITHMS FOR AUTOMATIC DEBLURRIng FROM A SIngLE IMAGE

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Journal of Computational Mathematics 2012年第1期30卷 80-100页

作者： wei Wang Michael K.ng Department of Mathematics Tongji UniversityShanghai 200092China Centre for Mathematical Imaging and Vision and Department of Mathematics Hong Kong Baptist UniversityKowloon TongHong KongChina

In this paper, we study two variational blind deblurring models for a single linage,The first model is to use the total variation prior in both image and blur, while the second model is to use the flame based prior in both image and blur. The main contribution of this paper is to show how to employ the generalized cross validation （GCV） method efficiently and automatically to estimate the two regularization parameters associated with the priors in these two blind motion deblurring models. Our experimental results show that the visual quality of restored images by the proposed method is very good, and they are competitive with the tested existing methods. We will also demonstrate the proposed method is also very efficient.

关键词： Blind deconvolution Iterative methods Total variation Framelet Generalizedcross validation.

Multi-Phase Texture Segmentation Using Gabor Features Histograms Based on Wasserstein Distance

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Communications in Computational Physics 2014年第5期15卷 1480-1500页

作者： Motong Qiao wei Wang Michael ng Department of Mathematics Hong Kong Baptist UniversityKowloon TongHong Kong Department of Mathematics Tongji UniversityShanghai 200092China Centre for Mathematical Imaging and Vision and Department of Mathematics Hong Kong Baptist UniversityKowloon TongHong Kong.

We present a multi-phase image segmentation method based on the histogram of the Gabor feature space,which consists of a set of Gabor-filter responses with various orientations,scales and *** model replaces the error function term in the original fuzzy region competition model with squared 2-Wasserstein distance function,which is a metric to measure the distance of two *** energy functional is minimized by alternative minimization method and the existence of closed-form solutions is guaranteed when the exponent of the fuzzy membership term being 1 or *** test our model on both simple synthetic texture images and complex natural images with two or more *** results are shown and compared to other recent results.

关键词： Multi-phase texture segmentation Wasserstein distance Gabor filter Mumford-Shah model.

A Nonlocal Total Variation Model for Image Decomposition: Illumination and Reflectance

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Numerical Mathematics(Theory,Methods and Applications) 2014年第3期7卷 334-355页

作者： wei Wang Michael K.ng Department of Mathematics Tongji UniversityShanghaiChina Department of Mathematics Hong Kong Baptist UniversityKowloon TongHong Kong

In this paper,we study to use nonlocal bounded variation(NLBV)techniques to decompose an image intensity into the illumination and reflectance *** considering spatial smoothness of the illumination component and nonlocal total variation(NLTV)of the reflectance component in the decomposition framework,an energy functional is *** establish the theoretical results of the space of NLBV functions such as lower semicontinuity,approximation and *** essential properties of NLBV functions are important tools to show the existence of solution of the proposed energy *** results on both grey-level and color images are shown to illustrate the usefulness of the nonlocal total variation image decomposition model,and demonstrate the performance of the proposed method is better than the other testing methods.

关键词： Image decomposition illumination reflectance nonlocal total variation iterative method

MODIFIED NEWTON＇S ALGORITHM FOR COMPUting THE GROUP INVERSES OF SIngULAR TOEPLITZ MATRICES

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Journal of Computational Mathematics 2006年第5期24卷 647-656页

作者： Jian-feng Cai Michael K. ng Yi-min wei Department of Mathematics The Chinese University of Hong Kong Hong Kong Department of Mathematics Hong Kong Baptist University Hong Kong School of Mathematical Sciences Fudan University Shanghai 200433 China ~ Key Laboratory of Mathematics for Nonlinear Sciences (Fudan University) Ministry of Education

Newton＇s iteration is modified for the computation of the group inverses of singular Toeplitz matrices. At each iteration, the iteration matrix is approximated by a matrix with a low displacement rank. Because of the displacement structure of the iteration matrix, the matrix-vector multiplication involved in Newton＇s iteration can be done efficiently. We show that the convergence of the modified Newton iteration is still very fast. Numerical results are presented to demonstrate the fast convergence of the proposed method.

关键词： Newton's iteration Group inverse Toeplitz matrix Displacement rank.

Modeling the high-energy emission from the gamma-ray binary 1FGL J1018.6–5856

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Research in Astronomy and Astrophysics 2021年第8期21卷 46-58页

作者： A-Ming Chen Chowing ng Jumpei Takata Yun-wei Yu School of physics Huazhong University of Science and TechnologyWuhan 430074China Department of Physics The University of Hong KongPokfulam RoadHong KongChina College of Physical Science and Technology Central China Normal UniversityWuhan 430079China

1FGL J1018.6–5856 is a high mass gamma-ray binary containing a compact object orbiting around a massive star with a period of 16.544 *** the compact object is a pulsar,non-thermal emissions are likely produced by electrons accelerated at the termination shock,and may also originate from the magnetosphere and the un-shocked wind of the *** this paper,we investigate the non-thermal emissions from the wind and the shock with different viewing geometries and study the multi-wavelength emissions from 1FGL J1018.6–*** present the analysis results of the Fermi/LAT using nearly 10 years of *** phase-resolved spectra indicate that the Ge V emissions comprise a rather steady component that does not vary with orbital motion and a modulated component that shows flux maximum around inferior *** ke V/Te V light curves of 1FGL J1018.6–5856 also exhibit a sharp peak around inferior conjunction,which are attributed to the boosted emission from the shock,while the broad sinusoidal modulations could be originating from the deflected shock tail at a larger *** modulations of Ge V flux are likely caused by the boosted synchrotron emission from the shock and the IC emission from the unshocked pulsar wind,while the steady component comes from the outer gap of the pulsar ***,we discuss the similarities and differences of 1FGL J1018.6–5856 with other binaries,like LS 5039.

关键词： binaries:close gamma rays:stars X-rays:binaries radiation mechanisms:non-thermal

Pedicled omental patch as a bridging procedure for iatrogenic bile duct injury

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World Journal of Gastroenterology 2017年第36期23卷 6741-6746页

作者： Jun Jie ng Alfred wei Chieh Kow Department of Surgery National University HospitalSingapore 119074 Singapore

Iatrogenic bile duct injuries during cholecystectomy can present as fulminant intra-abdominal sepsis which precludes immediate repair or biliary *** report the case of a 29-year-old female patient who sustained a bile duct injury after an open cholecystectomy in a neighboring *** presented to our institution 22 d after initial surgery with septic shock and multiple intra-abdominal *** retrograde cholangiography revealed a large common hepatic duct defect corresponding to a Strasberg type D bile duct *** reconstruction such as a hepaticojejunostomy cannot be performed due to the presence of dense adhesions with infected and friable *** underwent a combination of endoscopic biliary stenting and pedicled omental patch repair of the bile duct to control bile leak and sepsis as a bridging procedure to definite hepaticojejunostomy three months later.

关键词： Cholecystectomy Endoscopic retrograde cholangiopancreatography Case reports Bile ducts Abdominal abscess

Application of artifcial intelligence in cataract management:current and future directions

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Eye and Vision 2024年第1期10卷 21-30页

作者： Laura Gutierrez Jane Sujuan Lim Li Lian Foo wei Yan ng Michelle Yip Gilbert Yong San Lim Melissa Hsing Yi Wong Allan Fong Mohamad Rosman Jodhbir Singth Mehta Haotian Lin Darren Shu Jeng ting Daniel Shu wei ting Singapore Eye Research Institute SingaporeSingapore Singapore National Eye Center 11 Third Hospital AvenueSingapore 168751Singapore Academic Ophthalmology School of MedicineUniversity of NottinghamNottinghamUK Zhongshan Ophthalmic Center Sun Yet Sen UniversityGuangzhouChina

The rise of artifcial intelligence(AI)has brought breakthroughs in many areas of *** ophthalmology,AI has delivered robust results in the screening and detection of diabetic retinopathy,age-related macular degeneration,glaucoma,and retinopathy of *** management is another feld that can beneft from greater AI *** is the leading cause of reversible visual impairment with a rising global clinical *** diagnosis,monitoring,and surgical management are necessary to address this *** addition,patients in large developing countries often sufer from limited access to tertiary care,a problem further exacerbated by the ongoing COVID-19 *** on the other hand,can help transform cataract management by improving automation,efcacy and overcoming geographical ***,AI can be applied as a telediagnostic platform to screen and diagnose patients with cataract using slit-lamp and fundus *** utilizes a deep-learning,convolutional neural network(CNN)to detect and classify referable cataracts ***,some of the latest intraocular lens formulas have used AI to enhance prediction accuracy,achieving superior postoperative refractive results compared to traditional ***,AI can be used to augment cataract surgical skill training by identifying diferent phases of cataract surgery on video and to optimize operating theater workfows by accurately predicting the duration of surgical ***,some AI CNN models are able to efectively predict the progression of posterior capsule opacifcation and eventual need for YAG laser *** advances in AI could transform cataract management and enable delivery of efcient ophthalmic *** key challenges include ethical management of data,ensuring data security and privacy,demonstrating clinically acceptable performance,improving the generalizability of AI models across heterogeneous populations,and improving the trust of e

关键词： Artifcial intelligence Telemedicine Cataract Cataract screening Cataract surgery IOL calculations Biometry Machine learning