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长链非编码RNA KIAA0125对急性髓系白血病U937细胞增殖和凋亡的影响

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中国组织工程研究 2025年第19期29卷 3983-3991页

作者：胡华丽邓发滑刘远程王斯奇张静馨禄婷婷黄海韦四喜贵州医科大学附属医院临床检验中心贵州省贵阳市550004 贵州医科大学医学检验学院贵州省贵阳市550004

背景:U937细胞可以作为急性髓系白血病细胞模型,用于研究急性髓系白血病的生物学特性、信号通路和治疗靶点。目前虽然已有研究报道长链非编码RNA KIAA0125在急性髓系白血病中呈高表达,但其在U937细胞中的生物学功能尚不清楚,在急性髓系... 详细信息

背景:U937细胞可以作为急性髓系白血病细胞模型,用于研究急性髓系白血病的生物学特性、信号通路和治疗靶点。目前虽然已有研究报道长链非编码RNA KIAA0125在急性髓系白血病中呈高表达,但其在U937细胞中的生物学功能尚不清楚,在急性髓系白血病发生发展中的作用机制有待进一步阐明。目的:探讨长链非编码RNA KIAA0125在急性髓系白血病患者外周血中的表达水平及对U937细胞增殖、凋亡的影响。方法:RNA-seq分析急性髓系白血病患者骨髓单核细胞样本,筛选得到差异表达基因——长链非编码RNA KIAA0125,利用qRT-PCR检测长链非编码RNA KIAA0125在急性髓系白血病患者外周血中的表达进行验证,通过GEPIA数据库统计分析173例急性髓系白血病患者和70例健康人骨髓细胞中长链非编码RNA KIAA0125 mRNA的表达与预后的关系。随后使用重组慢病毒技术及CRISPR/Cas9-SAM技术分别构建敲低/过表达长链非编码RNA KIAA0125的U937细胞系,qRT-PCR检测长链非编码RNA KIAA0125敲低/过表达效率。接下来,使用CCK-8实验、流式细胞术及Western blot检测敲低/过表达长链非编码RNA KIAA0125对U937细胞增殖、凋亡的影响。最后,使用Western blot检测敲低/过表达长链非编码RNA KIAA0125对Wnt/β-catenin信号通路相关蛋白的影响。结果与结论:①qRT-PCR结果显示长链非编码RNA KIAA0125在急性髓系白血病患者外周血中呈高表达,GEPIA数据库显示长链非编码RNA KIAA0125在急性髓系白血病患者骨髓细胞中呈高表达,高表达组具有更差的生存期;②敲低组长链非编码RNA KIAA0125的敲低效率为70%,成功构建了稳定敲低长链非编码RNA KIAA0125表达的U937细胞,过表达组长链非编码RNA KIAA0125的表达是Vector组的4倍,成功构建了稳定过表达长链非编码RNA KIAA0125的U937细胞;③敲低长链非编码RNA KIAA0125抑制U937细胞的增殖并促进其凋亡,过表达长链非编码RNA KIAA0125则促进U937细胞的增殖但对U937细胞的凋亡无显著影响;④敲低长链非编码RNA KIAA0125抑制Wnt/β-catenin信号通路活性,而过表达长链非编码RNA KIAA0125则激活Wnt/β-catenin信号通路。结果表明,长链非编码RNA KIAA0125在急性髓系白血病外周血中呈高表达,其可能通过调控Wnt/β-catenin信号通路影响U937细胞的增殖和凋亡,可能是急性髓系白血病的潜在预后标志物。

关键词：急性髓系白血病 lncKIAA0125 Wnt/β-catenin U937细胞增殖

Over-expression of heme oxygenase-1 in peripheral blood predicts the progression and relapse risk of chronic myeloid leukemia

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Chinese Medical Journal 2014年第15期127卷 2795-2801页

作者： wei sixi Wang Yating Chai Qixiang Fang Qin Zhang Yaming Lu Yinghao Wang Jishi Hematopoietic Stem Cell Transplantation Center of GuizhouProvince Department of Hematology Affiliated Hospital of Guiyang Medical College Guiyang Guizhou550004 China Department of Clinical Biochemistry Affiliated Hospital of Guiyang Medical College Guiyang Guizhou550004 China Department of Pharmacy Affiliated Hospital of Guiyang Medical College Guiyang Guizhou550004 China

Background There are limited eligible clinical markers at present to monitor the progress of chronic myeloid leukemia （CML）.Heme oxygenase-1 （HO-1）,as one of the most important oxidation-regulating enzymes in vivo,suggests the onset and progression of cancer when highly ***,HO-1 level is related with the occurrence and development of hematological *** the relationship between HO-1 expression and progression/relapse of CML has seldom been studied *** study aimed to investigate the relationship between them to find out a new molecular marker for *** A total of 60 peripheral blood and bone marrow （BM） samples from 25 CML patients in different phases were collected respectively to detect the expressions of HO-1 and bcr/abl using real-time *** blood test was performed to detect the changes of leukocyte and platelet *** proportion of primitive cells in BM was detected by flow *** relationship between high HO-1 expression and CML progression and relapse was explored by the analysis of variance by Wilcoxon test and linear regression *** diagnostic accuracy and cutoff values were determined by receiver operating characteristic *** Relative expression of HO-1 mRNA in CML patients peripheral blood was significantly higher than that of donors （P 〈0.0001）,which were 0.57±3.78 and （1.417±1.125）×10-6,***-1 expression level in CML patients was 0.061 5±0.062 4,which decreased to 0.009 4±0.006 7 upon CMoR,and remained remarkably higher 0.016 3±0.017 5 than that of normal donors （1.417±1.125）× 10-6,P 〈*** relapse occurred,HO-1 expression significantly increased from 0.020 6±0.021 0 to 3.852±10.285 in CMoR stage and undergoing *** to progression of CML,HO-1 expression level in CML patients increased from CP （0.009 5±0.017 6） to AP （0.028 0±0.055 7） and then to BP （0.276 7± 0.447 0）.And there was a linear correlation between HO-1 expression and proportion of p

关键词： heme oxygenase-1 chronic myeloid leukemia progression relapse receiver operating characteristic curve

Molecular Dynamics-Based Simulation of Polyethylene Pipe Degradation in High Temperature and High Pressure Conditions

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Fluid Dynamics & Materials Processing 2024年第9期20卷 2139-2161页

作者： Guowei Feng Qing Li Yang Wang Nan Lin sixi Zha Hang Dong Ping Chen Minjun Zheng School of Mechanical Engineering Xinjiang UniversityUrumqi830046China Xinjiang Yian Special Inspection Engineering Company Limited Urumqi830063China Xinjiang Uygur Autonomous Region Inspection Institute of Special Equipment Urumqi830000China Pressure Pipe Department China Special Equipment Inspection and Research InstituteBeijing100013China Tarim Oilfield Branch Company Korla841000China Yimai Cai Oil and Gas Management Area Tarim Oilfield Branch OfficeKorla841000China

High-density polyethylene(HDPE)pipes have gradually become the first choice for gas networks because of their excellent *** the use of pipes increases,there will unavoidably be a significant amount of waste generated when the pipes cease their operation life,which,if improperly handled,might result in major environmental contamination *** this study,the thermal degradation of polyethylene materials is simulated for different pressures(10,50,100,and 150 MPa)and temperatures(2300,2500,2700,and 2900 K)in the framework of Reactive Force Field(ReaxFF)molecular dynamics *** main gas products,density,energy,and the mean square displacement with temperature and pressure are also *** findings indicate that raising the temperature leads to an increase in the production of gas products,while changing the pressure has an impact on the direction in which the products are generated;the faster the temperature drops,the less dense the air;both temperature and pressure increase impact the system’s energy conversion or distribution mechanism,changing the system’s potential energy as well as its total energy;the rate at which molecules diffuse increases with temperature,and decreases with *** results of this investigation provide a theoretical basis for the development of the pyrolytic treatment of polyethylene waste materials.

关键词： ReaxFF MD high-density polyethylene degradation temperature pressure

Screening and expression analysis of lncRNAs in high-risk MDS

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Screening and expression analysis of lncRNAs in high-risk MD...

A low-frequency and broadband wave-insulating vibration isolator based on plate-shaped metastructures

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： wei,sixi Huang,Yanjie Ma,Yucan Zhao,Juanjuan Ma,Li Kong,weiying Bi,Ying Huang,Hai Division of Clinical Biochemistry and Molecular Diagnostics in Center for Clinical Laboratory Medicine Affiliated Hospital to Guizhou Medical University

Objection:Aimed to screen out the differentially expressed lncRNAs and verify its expression,exploring lncRNAs marker for diagnosis and therapeutics relevant to ***:Three cases bone marrow from patients with high-risk MDS and three healthy donors were collected respectively for lncRNA microarray;bioinformatics analysis was performed to predict the potential interactional targets of lncRNA;Peripheral blood of 20 patients with high-risk MDS and 20 healthy donors were employed for expression verification and co-expression analysis by SYBR-green ***:Microarray profiling indicated 150 differentially expressed lncRNAs in bone marrow of patients with high-risk MDS,of which 119 lncRNAs showed *** CDKN2-AS as a study target,which over-expressed in MDS with 3.27 folds of logFC compared to healthy *** analysis showed that RUNX1 was a potential target of mutual effect to CDKN2-AS;CDKN2-AS was highly expressed in 20 high-risk MDS patients,with a relative expression of 49.74±109.55,while low expressed in healthy donors with a relative expression of 0.56±2.35,significant difference was showed between the two groups,p<0.01;while RUNX1 was low expressed in high-risk MDS patients,the relative expression level indicated significant difference in MDS and healthy donors,p < ***:CDKN2-AS is a potential marker of MDS and its’ expression is associated with the prognosis of MDS.

关键词： Myelodysplastic syndrome long-chain non-coding RNA lncRNA CDKN2-AS RUNX1 bioinformatics analysis

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Applied Mathematics and Mechanics(English Edition) 2024年第7期45卷 1171-1188页

作者： wei wei Feng GUAN Xin FANG College of Intelligent Science National University of Defense TechnologyChangsha410073China Naval Research Academy Beijing100161China

A metamaterial vibration isolator,termed as wave-insulating isolator,is proposed,which preserves enough load-bearing capability and offers ultra-low and broad bandgaps for greatly enhanced wave *** consists of plate-shaped metacells,whose symmetric and antisymmetric local resonant modes offer several low and broad mode bandgaps although the complete bandgap remains high and *** bandgap mechanisms,vibration isolation properties,effects of key parameters,and robustness to complex conditions are *** experimentally demonstrated,the wave-insulating isolator can improve the vibration insulation in the ranges of[50 Hz,180 Hz]and[260 Hz,400 Hz]by 15 dB and 25 dB,respectively,in contrast to the conventional isolator with the same first resonant frequency.

关键词： metamaterial and metastructure vibration isolation bandgap wave insulation plate

Spin-orbit torque efficiency enhancement to tungsten-based SOT-MTJs by interface modification with an ultrathin MgO

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Science China(Information Sciences) 2024年第1期67卷 316-317页

作者： Shiyang LU Xiaobai NING Hongchao ZHANG sixi ZHEN Xiaofei FAN Danrong XIONG Dapeng ZHU Gefei WANG Hong-Xi LIU Kaihua CAO weisheng ZHAO Fert Beijing Institute School of Integrated Science and EngineeringBeihang University Truth Memory Corporation

Spin-orbital torque (SOT) based three-terminal magnetic tunnel junctions (MTJs) have attracted much interest as the next generation magnetic random-access memory (MRAM)key device both in the academy and the industry. This is because SOT-MTJs feature high endurance and fast switching speed, benefiting tremendously from the separation of read/write current paths and less incubation time during the write in contrast to the spin-transfer torque (STT) MTJs [1].

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Planet wind: Planetary evolution dominated by electromagnetic forces

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Science China Earth Sciences 2024年第10期67卷 3331-3336页

作者： Yong wei Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

1. Introduction Over the five centuries since Copernicus, the focus of planetary science has transitioned from the visible realm to the invisible. In the more than 60 years following the advent of the space age, our a... 详细信息

关键词： evolution dominated planetary

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The lithology and composition of lunar mantle modified by ilmenite bearing cumulate:A thermodynamic model

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Acta Geochimica 2024年第5期43卷 856-875页

作者： wei Huang wei Du State Key Laboratory of Ore Deposit Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Comparative Planetology Chinese Academy of SciencesHefei 230026China

Due to their high density,the ilmenite-bearing cumulates(IBC)(with or without KREEP)formed during the late-stage lunar magma ocean solidification are thought to sink into the underlying lunar mantle and trigger lunar mantle *** evidence implied that IBC may descend deep inside the Moon and remain as a partially molten layer at the core-mantle boundary(CMB).However,partial melting may have occurred on the mixed mantle cumulates during the sinking of IBC/KREEP and the silicate melt may be positively buoyant,thus preventing the IBC/KREEP layer from sinking to the ***,we perform thermodynamic simulation on the stability of lunar mantle cumulates at different depths mixed with different amounts of IBC/KREEP from an updated LMO *** modeling results suggest that the sinking of IBC/KREEP will cause at least 5 wt%partial melting in the shallow(~120 km)and a much larger degree of partial melting in the deep lunar mantle(~420 km).Due to the density contrast with the surrounding mantle,IBC/KREEP-bearing melts could potentially decouple under certain *** modified lunar mantle by sinking of IBC/KREEP can better explain the formation of different kinds of lunar basaltic magma than the primary lunar mantle formed through differentiation of lunar magma *** of IBC/KREEP back into the lunar mantle may introduce plagioclase,clinopyroxene,garnet,and incompatible radioactive elements into the deep lunar mantle,which will further affect the thermal and chemical evolution of the lunar interior.

关键词： Ilmenite-bearing cumulate KREEP Partial melting Lunar mantle overturn pMELTS

Task-specific Part Discovery for Fine-grained Few-shot Classification

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Machine Intelligence Research 2024年第5期21卷 954-965页

作者： Yongxian wei Xiu-Shen wei School of Computer Science and Technology Nanjing University of Science and TechnologyNanjing210094China

Localizing discriminative object parts(e.g.,bird head)is crucial for fine-grained classification tasks,especially for the more challenging fine-grained few-shot *** work always relies on the learned object parts in a unified manner,where they attend the same object parts(even with common attention weights)for different few-shot episodic *** this paper,we propose that it should adaptively capture the task-specific object parts that require attention for each few-shot task,since the parts that can distinguish different tasks are naturally *** for a few-shot task,after obtaining part-level deep features,we learn a task-specific part-based dictionary for both aligning and reweighting part features in an ***,part-level categorical prototypes are generated based on the part features of support data,which are later employed by calculating distances to classify query data for *** retain the discriminative ability of the part-level representations(i.e.,part features and part prototypes),we design an optimal transport solution that also utilizes query data in a transductive way to optimize the aforementioned distance calculation for the final *** experiments on five fine-grained benchmarks show the superiority of our method,especially for the 1-shot setting,gaining 0.12%,8.56%and 5.87%improvements over state-of-the-art methods on CUB,Stanford Dogs,and Stanford Cars,respectively.

关键词： Fine-grained image recognition few-shot learning transductive learning visual dictionary part feature discovery