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Green synthesis,antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract

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Asian Pacific Journal of Tropical Biomedicine 2013年第1期3卷 58-63页

作者： Ghassan Mohammad Sulaiman wasnaa hatif mohammed Thorria Radam Marzoog Ahmed Abdul Amir Al-Amiery Abdul Amir H.Kadhum Abu Bakar Mohamad Biotechnology Division Applied Science DepartmentUniversity of Technology Applied Chemistry Division Applied Science DepartmentUniversity of Technology Chemical and Process Engineering Department Universiti Kebangsaan Malaysia

Objective:To synthesize silver nanopaticles from leaves extract of Eucalyptus chapmaniana(***)and test the antimicrobial of the nanoparticles against different pathogenic bacteria,yeast and its toxicity against human acute promyelocytic leukemia(HL-60)cell ***:Ten milliliter of leaves extract was mixed with 90 mL of 0.01 mmol/mL or 0.02 mmol/mL aqueous AgNO_3 and exposed to sun light for 1 h.A change from yellowish to reddish brown color was *** using UV-vis spectrophotometery and X-ray diffraction analysis were *** activity against six microorganisms was tested using well diffusion method and cytoxicity test using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,a yellow tetrazole was obtained on the human leukemia cell line(HL-60).Results:UV-vis spectral analysis showed silver surface plasmon resonance band at 413 nm.X-ray diffraction showed that the particles were crystalline in nature with face centered cubic structure of the bulk silver with broad beaks at 38.50°and 44.76°.The synthesized silver nanoparticles efficiently inhibited various pathogenic organisms and reduced viability of the HL-60 cells in a dose-dependent ***:It has been demonstrated that the extract of *** leaves are capable of producing silver nanoparticles extracellularly and the Ag nanoparticles are quite stable in *** studies are needed to fully characterize the toxicity and the mechanisms involved with the antimicrobial and anticancer activity of these particles.

关键词： Eucalyptus chapmaniana Silver nanoparticles Antimicrobial activity Cytotoxic effect

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