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The changes of Trp-deficient linusorb during the oxidation and the influence of γ-tocopherol on their oxidative stability

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Grain & Oil Science and Technology 2024年第4期7卷 219-228页

作者： Dujian Deng Jule li Jiahui Yu wanzhen li Martin J.T.Reaney Zizhe Cai Yong Wang Guangdong Saskatchewan Oilseed Joint Laboratory Department of Food Science and EngineeringJinan UniversityGuangzhou 510632China Department of Plant Sciences University of Saskatchewan51 Campus Dr.SaskatoonCanada

linusorbs(LOs),particularly the Methionine(Met)-containing yet Tryptophan(Trp)-free LO(CLB and CLP),play a significant role in shaping the quality and sensory attributes of flaxseed *** investigate the stability of these LOs and their oxidized forms during accelerated oxidation,the polar fractions of crude flaxseed oil were selectively removed using silica absorption and then reintroduced separately into the oil matrix along with LOs andγ-tocopherol(γ-T).Through comprehensive analysis of oxidative stability indexes,LOs content changes,oxidation kinetics,and the correlation between LOs and oxidative stability indexes,we observed that the LOs fraction alone exhibited a moderate antioxidant effect on the peroxide value(PV)but a weaker effect on the panisidine value(p-AV)of flaxseed ***,upon addition ofγ-T,we observed a weak synergistic effect betweenγ-T and LOs in suppressing PV,while a stronger effect in suppressing ***,a comparison of the oxidation kinetics of different LOs indicated thatγ-T preferentially protected the oxidation of CLP over *** protective trends ofγ-T towards the methionine sulfoxide(MetO)and methionine sulfoxide(MetO2)form of LOs were also observed,albeit to a lesser *** analysis revealed that changes in Metcontaining LOs,particularly CLP,showed a high correlation with PV in the tested matrices,while MetOcontaining LOs better depicted changes in ***,CLP demonstrated higher susceptibility to the influence of antioxidants,making it more suitable for evaluating the peroxide value in flaxseed oil samples.

关键词： Flaxseed oil Cyclolinopeptide γ-Tocopherol Oxidative stability

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One-step synthesis of site-specific antibody-drug conjugates by reprograming IgG glycoengineering with LacNAc-based substrates

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2417-2428页

作者： Wei Shi wanzhen li Jianxin Zhang Tiehai li Yakai Song Yue Zeng Qian Dong Zeng lin likun Gong Shuquan Fan Feng Tang Wei Huang CAS Key Laboratory of Receptor Research CAS Center for Excellence in Molecular Cell ScienceCenter for Biotherapeutics Discovery ResearchShanghai Institute of Materia Medica Chinese Academy of SciencesShanghai 201203China School of Pharmaceutical Science and Technology Hangzhou Institute of Advanced StudyHangzhou 310024China University of Chinese Academy of Sciences Beijing 100049China School of Chinese Materia Medica Nanjing University of Chinese MedicineNanjing 210023China School of life Science Liaocheng UniversityLiaocheng 252059China

Glycosite-specific antibody-drug conjugatess(gsADCs), harnessing Asn297 N-glycan of IgG Fc as the conjugation site for drug payloads, usually require multi-step glycoengineering with two or more enzymes, which limits the substrate diversification and complicates the preparation ***, we report a series of novel disaccharide-based substrates, which reprogram the IgG glycoengineering to one-step synthesis of gsADCs, catalyzed by an endo-N-acetylglucosaminidase(ENGase) of Endo-S2. IgG glycoengineering via ENGases usually has two steps: deglycosylation by wild-type(WT) ENGases and transglycosylation by mutated ENGases. But in the current method, we have found that disaccharide LacNAc oxazoline can be efficiently assembled onto IgG by WT Endo-S2 without hydrolysis of the product, which enables the one-step glycoengineering directly from native *** studies on substrate specificity revealed that this approach has excellent tolerance on various modification of 6-Gal motif of LacNAc. Within 1 h, one-step synthesis of gsADC was achieved using the LacNAc-toxin substrates including structures free of bioorthogonal groups. These gsADCs demonstrated good homogeneity, buffer stability, in vitro and in vivo anti-tumor activity. This work presents a novel strategy using LacNAc-based substrates to reprogram the multi-step IgG glycoengineering to a one-step manner for highly efficient synthesis of gsADCs.

关键词： Site-specific ADCs ENGase LacNAc One-step assembly Potent in vivo efficacy

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Association of TRMT2B gene variants with juvenile amyotrophic lateral sclerosis

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Frontiers of Medicine 2024年第1期18卷 68-80页

作者： Yanling liu Xi He Yanchun Yuan Bin li Zhen liu wanzhen li Kaixuan li Shuo Tan Quan Zhu Zhengyan Tang Feng Han Ziqiang Wu Lu Shen Hong Jiang Beisha Tang Jian Qiu Zhengmao Hu Junling Wang Department of Neurology Xiangya HospitalCentral South UniversityJiangxiNational Regional Center for Neurological DiseasesNanchang330038China Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease Department of UrologyXiangya HospitalCentral South UniversityChangsha410078China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310002China Department of Neurology Xiangya HospitalCentral South UniversityChangsha410078China National Clinical Research Center for Geriatric Diseases Xiangya HospitalCentral South UniversityChangsha410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangsha410008China Center for Medical Genetics School of Life SciencesCentral South UniversityChangsha410008China Engineering Research Center of Hunan Province in Cognitive Impairment Disorders Central South UniversityChangsha410078China Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases Changsha410078China Hunan Key Laboratory of Molecular Precision Medicine Xiangya HospitalCentral South UniversityChangsha410078China

Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons,and it demonstrates high clinical heterogeneity and complex genetic architecture.A variation within TRMT2B(c.1356G>T;p.K452N)was identified to be associated with ALS in a family comprising two patients with juvenile ALS(JALS).Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS,and three more variants were identified in a public ALS database including 3317 patients with ALS.A decreased number of mitochondria,swollen mitochondria,lower expression of ND1,decreased mitochondrial complex I activities,lower mitochondrial aerobic respiration,and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 ***,TRMT2B variations overexpression cells also displayed decreased *** conclusion,a novel JALS-associated gene called TRMT2B was identified,thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B amyotrophic lateral sclerosis mitochondrial complex I tRNA methylation reactive oxygen species

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Association of variants in the KIF1A gene with amyotrophic lateral sclerosis

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Translational Neurodegeneration 2022年第1期11卷 235-247页

作者： Panlin liao Yanchun Yuan Zhen liu Xiaorong Hou wanzhen li Jin Wen Kexuan Zhang Bin Jiao Lu Shen Hong Jiang Jifeng Guo Beisha Tang Zhuohua Zhang Zhonghua Hu Junling Wang Hunan Key Laboratory of Molecular Precision Medicine Department of Critical Care MedicineXiangya HospitalCentral South UniversityChangsha 410008China Department of Neurology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Diseases Xiangya HospitalCentral South UniversityChangsha 410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangsha 410008China Center for Medical Genetics School of Life SciencesCentral South UniversityChangsha 410008China Engineering Research Center of Hunan Province in Cognitive Impairment Disorders Central South UniversityChangsha 410008China Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases Changsha 410008China Hunan Provincial Clinical Research Center for Critical Care Medicine Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Animal Models for Human Diseases School of Life SciencesCentral South UniversityChangsha 410008China.

Background:Amyotrophic lateral sclerosis(ALS)is a devastating progressive neurodegenerative disease that affects neurons in the central nervous system and the spinal *** in many other neurodegenerative disorders,the genetic risk factors and pathogenesis of ALS involve dysregulation of cytoskeleton and neuronal ***,sen-sory and motor neuron diseases such as hereditary sensory and autonomic neuropathy type 2(HSAN2)and spastic paraplegia 30(SPG30)share several causative genes with ALS,as well as having common clinical ***1A encodes a kinesin 3 motor that transports presynaptic vesicle precursors(SVPs)and dense core vesicles and has been reported as a causative gene for HSAN2 and ***:Here,we analyzed whole-exome sequencing data from 941 patients with ALS to investigate the genetic association of KIF1A with ***:We identified rare damage variants(RDVs)in the KIF1A gene associated with ALS and delineated the clini-cal characteristics of ALS patients with KIF1A ***,these patients tended to exhibit sensory ***,the majority of these variants are located at the C-terminal cargo-binding region of the KIF1A *** examination revealed that the ALS-associated KIF1A variants located in the C-terminal region preferentially enhanced the binding of SVPs containing RAB3A,VAMP2,and *** of several disease-related KIF1A mutants in cultured mouse cortical neurons led to enhanced colocalization of RAB3A or VAMP2 with the KIF1A ***:Our study highlighted the importance of KIF1A motor-mediated transport in the pathogenesis of ALS,indicating KIF1A as an important player in the oligogenic scenario of ALS.

关键词： Amyotrophic lateral sclerosis KIF1A Axonal transport Presynaptic vesicle precursors

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黑龙江流域满族先民的农业文明

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黑龙江民族丛刊 2006年第6期 79-82页

作者：李宛真黑龙江省博物馆宣教部黑龙江哈尔滨150001

本文利用丰富的历史文献和考古资料,论述了黑龙江流域满族先民对黑龙江流域农业文明的历史性贡献。

关键词：黑龙江流域满族先民农业文明

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人工智能对国际法的挑战及其应对

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浙江大学学报（人文社会科学版） 2020年第2期50卷 10-25页

作者：赵骏李婉贞浙江大学光华法学院浙江杭州310008

人工智能技术的迅猛发展使国际交往更加便利和频繁,但也对现有国际法律体系提出了新的挑战。国际法基本原则中的国家主权原则、国家责任原则以及国际法具体领域中的国际法居民制度、国际人道法、国际刑法、国际知识产权法、国际人权法... 详细信息

人工智能技术的迅猛发展使国际交往更加便利和频繁,但也对现有国际法律体系提出了新的挑战。国际法基本原则中的国家主权原则、国家责任原则以及国际法具体领域中的国际法居民制度、国际人道法、国际刑法、国际知识产权法、国际人权法、国际海洋法与国际海事法等,都受到了人工智能技术发展的影响,面临严峻的挑战。作为维护国际和平、促进国际社会共同发展的国际法,应当与时俱进,积极应对国际智能社会的各种挑战。为此,国家之间应当加强合作,以人工智能的安全使用为目标,通过制定国际软法、缔结多边条约、设立专门性国际机构等方式,引导人工智能技术健康发展,解决新科技带来的国际新问题。

关键词：人工智能法律规制国际软法多边条约

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加强对川崎病诊治的研究

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中华儿科杂志 2002年第2期40卷 65-67页

作者：李万镇北京大学第一医院儿科 100034

川畸病（Kawasaki disease, KD）又名皮肤粘膜淋巴结综合征（MCLS），1967年日本川崎首先报告，主要病理改变为全身性非特异性血管炎，多侵犯冠状动脉，部分患儿形成冠状动脉瘤，其中少部分患儿冠状动脉可发生狭窄或血栓，甚至导致心肌... 详细信息

川畸病（Kawasaki disease, KD）又名皮肤粘膜淋巴结综合征（MCLS），1967年日本川崎首先报告，主要病理改变为全身性非特异性血管炎，多侵犯冠状动脉，部分患儿形成冠状动脉瘤，其中少部分患儿冠状动脉可发生狭窄或血栓，甚至导致心肌梗死。

关键词：川畸病儿童诊断治疗

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自适应波束形成算法

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信息与控制 2015年第2期44卷 165-170页

作者：焦圣喜王睿李婉珍东北电力大学自动化学院吉林吉林132012

针对线性约束最小方差(linearly constrained minimum variance,LCMV)自适应算法中存在的导向矢量误差敏感、协方差矩阵求逆计算量大等问题,提出一种改进的LCMV波束形成方法.通过变步长的最小均方(least mean square,LMS)算法求解最优... 详细信息

针对线性约束最小方差(linearly constrained minimum variance,LCMV)自适应算法中存在的导向矢量误差敏感、协方差矩阵求逆计算量大等问题,提出一种改进的LCMV波束形成方法.通过变步长的最小均方(least mean square,LMS)算法求解最优权值矢量,避免了求逆运算,降低了固定步长对算法性能的影响,采用4阶累积算法解决了假设导向矢量存在误差的问题.仿真结果表明,变步长LMS-LCMV算法不但能自适应地估计导向矢量且具有更快的收敛速度和较小的稳态误差.

关键词：自适应波束形成线性约束最小方差算法最小均方算法导向矢量

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垂直有序介孔二氧化硅的离子选择渗透性研究

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浙江大学学报（理学版） 2015年第4期42卷 440-444页

作者：王巧红李婉珍苏彬浙江大学化学系浙江杭州310058

采用Stber溶液生长法,在氧化铟锡(ITO)导电基底上合成了孔道高度有序且垂直于基底的介孔二氧化硅薄膜(Mesoporous silica film,MSF),其孔径约为2.3nm.利用循环伏安法探究了MSF孔道对带不同电荷的探针分子的选择渗透性;此外,利用纳米... 详细信息

采用Stber溶液生长法,在氧化铟锡(ITO)导电基底上合成了孔道高度有序且垂直于基底的介孔二氧化硅薄膜(Mesoporous silica film,MSF),其孔径约为2.3nm.利用循环伏安法探究了MSF孔道对带不同电荷的探针分子的选择渗透性;此外,利用纳米通道的尺寸效应和电荷效应,研究了溶液的离子强度对MSF孔道选择渗透性的影响,设计了具有"开/关"效应的智能响应界面.

关键词：纳米通道介孔二氧化硅薄膜选择渗透性双电层离子强度

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大鼠心肌β-肾上腺素受体发育的研究

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中华儿科杂志 1998年第11期36卷 674-677页

作者：曾和平李万镇北京医科大学第一医院儿科

目的探讨发育因素对大鼠心肌β肾上腺素受体（βＡＲ）的影响。方法应用配基结合、酶活性测定，测定不同年龄段Ｗｉｓｔａｒ大鼠心肌βＡＲ密度、受体亚型、腺苷酸环化酶（ＡＣ）活性和对βＡＲ激动剂———异丙肾上腺素反应性... 详细信息

目的探讨发育因素对大鼠心肌β肾上腺素受体（βＡＲ）的影响。方法应用配基结合、酶活性测定，测定不同年龄段Ｗｉｓｔａｒ大鼠心肌βＡＲ密度、受体亚型、腺苷酸环化酶（ＡＣ）活性和对βＡＲ激动剂———异丙肾上腺素反应性以及低甲状腺素大鼠心肌βＡＲ密度。结果大鼠出生时心肌βＡＲ密度平均为１４．３ｆｍｏｌ／ｍｇ（蛋白），成年后平均为３１．６ｆｍｏｌ／ｍｇ（蛋白），心肌βＡＲ密度随年龄的增长而增加（Ｆ值为２３．４６，Ｐ＜０．０１）；β１／β２ＡＲ亚型相对比值，出生时为５１／４９，成年为６０／４０；心肌ＡＣ对βＡＲ激动剂———异丙肾上腺素的反应性，出生时为７６ｐｍｏｌ·ｍｇ－１·ｍｉｎ－１，成年为１２６ｐｍｏｌ·ｍｇ－１·ｍｉｎ－１，各年龄段的差异有显著意义（Ｆ值为８９．００，Ｐ＜０．０１）；ＡＣ基础活性和ＡＣ对ＡＣ激动剂Ｆｏｒｓｋｏｌｉｎ的反应性，各年龄段没有差异；β１ＡＲ拮抗剂抑制成年大鼠心肌ＡＣ对异丙肾上腺素的反应性，β２ＡＲ拮抗剂抑制新生和发育早期大鼠心肌ＡＣ对异丙肾上腺素的反应性；血清甲状腺素减低可使生后１周和成年大鼠心肌βＡＲ密度下降。结论大鼠心肌βＡＲ密度、β１／β２ＡＲ亚型比例、β?

关键词：受体肾上腺素能β 心肌发育

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