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Molecular evolution of nitrogen-containing compounds in highly mature organic matter: Implications for the hydrocarbon generation potential

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Science China Earth Sciences 2023年第4期66卷 781-796页

作者： Jingkun ZHANG Jian CAO Wenxuan HU Baoli XIANG Ni ZHOU wanyun ma School of Earth Sciences and Engineering MOE Key Laboratory of Surficial GeochemistryNanjing UniversityNanjing 210023China Research Institute of Experiment and Testing PetroChina Xinjiang Oilfield CompanyKaramay 834000China

Evaluating the hydrocarbon generation potential of highly mature organic matter is a key and critically challenging area of research in petroleum geochemistry. To explore this issue, we used negative ion electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry to investigate the molecular evolution of N-containing compounds in Carboniferous-lower Permian source rocks with a range of maturities in the northwestern Junggar Basin, China. The N1compounds formed from on-fluorescent chlorophyll catabolites(NCCs), which record the characteristics of the residual soluble organic matter. These components remain in the source rocks after hydrocarbon generation and expulsion, and enable evaluation of the hydrocarbon generation potential. The newly defined indexes of molecular evolution, which are the polymerization index P1([DBE 18+DBE 15]/[DBE 12+DBE 9]_N1) and alkylation index R1(RC_(6–35)/RC_(0–5)), combined with the vitrinite reflectance(VR_(o)) and paleo-salinity index(β-carotane/n Cmax), can identify the factors that control the evolution of highly mature organic matter. The main factor for source rocks deposited in a weakly saline environment is the maturity, but for a highly saline environment both the maturity and salinity are key factors. The high salinity inhibits the molecular polymerization of organic matter and extends the oil generation peak. Given the differences in the bio-precursors in saline source rocks, we propose a new model for hydrocarbon generation that can be used to determine the oil generation potential of highly mature organic matter.

关键词： Negative ion ESI-FT-ICR-MS N-containing compound Residual hydrocarbon generation potential Highly mature organic matter Deep oil-gas Junggar Basin

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VvMYB14 participates in melatonin-induced proanthocyanidin biosynthesis by upregulating expression of VvMYBPA1 and VvMYBPA2 in grape seeds

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Horticulture Research 2023年第2期10卷 214-226页

作者： Xiaoqian Zhang wanyun ma Xueqiang Guan Fei Wang Zongbao Fan Shiwei Gao Yuxin Yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine JinanShandong 250100China

This work demonstrated that melatonin increases continuously in seeds,particularly seed coats,during berry *** melatonin treatments significantly increased the proanthocyanidin(PA)content,partially through ethylene signaling,in seed ***14 expression exhibited patterns similar to melatonin accumulation over time,which was largely induced by melatonin treatment in seed coats during berry ***,VvMYB14 bound to the MBS element of the VvMYBPA1 promoter to activate ***14 overexpression largely upregulated expression of VvMYBPA1,VvMYBPA2 and VvLAR1 and increased the PA content in grape seed-derived *** increases in AtTT2 and AtBAN expression and PA content were found in VvMYB14-overexpressing Arabidopsis *** was also observed that VvMYB14 overexpression increased ethylene production and thereby induced expression of VvERF104,which bound to the ERF element of the VvMYBPA2 promoter and activated its ***,VvERF104 suppression reduced the VvMYB14 overexpression-induced increases in expression of VvMYBPA2 and VvLAR1 and PA *** experiments revealed that melatonin-induced increases in the expression of VvMYBPA1,VvMYBPA2,VvERF104 and VvLAR1 and PA accumulation were significantly reduced in VvMYB14-suppressing grape calli and ***,VvMYB14 mediates melatonin-induced PA biosynthesis by directly transactivating VvMYBPA1 expression and indirectly upregulating VvMYBPA2 expression via VvERF104.

关键词： melatonin thereby treatment

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Analyzing crude oils from the Junggar Basin(NW China) using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry(GC×GC-TOFMS)

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Acta Geochimica 2017年第1期36卷 66-73页

作者： Yuce Wang wanyun ma Ni Zhou Jiangling Ren Jian Cao State Key Laboratory for Mineral Deposits Research Department of Earth SciencesNanjing University Research Institute of Experiment and Testing PetroChina Xinjiang Oilfield Company

As a new technology of analyzing crude oils,comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry(GC×GCTOFMS) has received much research *** we present a case study in the Junggar Basin of NW *** show that the hydrocarbons,including saturates and aromatics,were all well-separated without large coelution,which cannot be realized by conventional one-dimensional *** GC×GC technique is especially effective for analyzing aromatics and low-to-middlemolecular-weight hydrocarbons,such as *** geochemical characteristics of crude oils in the study area were investigated through geochemical parameters extracted by GC×GC-TOFMS,improving upon the understanding obtained by ***,the work here represents a new successful application of GC×GCTOFMS,showing its broad usefulness in petroleum geochemistry.

关键词： Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry(GC×GC-TOFMS) Petroleum geochemistry Biomarkers Diamondoid Junggar Basin

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Significance of spatial organization of chromosomes in the progression of acute myeloid leukemia

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Chinese Journal of Cancer 2017年第5期36卷 241-244页

作者： Xueli Tian Yanfang Wang Dieyan Chen Xiaoyan Ke wanyun ma State Key Laboratory of Low-Dimensional Quantum Physics Department of Physics Tsinghua University Beijing 100084 P. R. China Department of Hematology Peking University Third Hospital Beijing 100191 P. R. China Collaborative Innovation Center of Quantum matter Beijing 100084 P. R.China

Leukemia ranks as one of the ten most fatal cancers [1].The mortality and incidence of this disease are associated with multiple factors, including environmental factors,sex, and age. Distinct genetic and chromosomal aberrations differentially affect the phenotype and prognosis of individuals with leukemia [2, 3]. The t(8;21)(q22;q22) translocation, which is observed in patients with acute myeloid leukemia with maturation (AML-M2, according to the French-American-British classification system), is characterized by the fusion of AML1 (acute myeloid leukemia factor 1, also referred to as RUNX1 [runt-related transcription factor 1]) on chromosome 21 and ETO (eight-twenty-one, also referred to as RUNX1T1 [runtrelated transcription factor 1, translocated to 1]) on chromosome 8. Although the t(8;21)(q22;q22) translocation is associated with a favorable prognosis, relapse remains the primary cause of treatment failure [4].

关键词： Significance organization leukemia

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Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

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Chinese Science Bulletin 2013年第26期58卷 3248-3255页

作者： TIAN Ning ZHANG Lu L DanYu ZHENG JingGao ma wanyun LI Ying State Key Laboratory of Low-Dimensional Quantum Physics Department of Physics Tsinghua University Department of Histology and Embryology School of Basic Medical Sciences Peking University Health Science Center

This paper investigated the age-related changes in the expression patterns of maintenance methyltransferase (DNMT1) and de novo methyltransferases (DNMT3a, 3b, 3L) and the chromosome architecture in in-vivo matured mouse oocytes using two-photon laser-scanning microscope. Our results showed that (1) DNMT1 and DNMT3a, 3b, 3L in the oocytes of pubertal mice were located in the cortical region of oocyte cytoplasm. In aging groups, DNMT1 was also located in the cortical region. However, DNMT3a, 3b, 3L had a relatively wider distribution in the oocyte cytoplasm and appeared near the chromosomes. These differences between pubertal and aging groups suggested that aging might affect DNA methylation; (2) the expression of DNMT1, and DNMT3a, 3b in aging groups increased significantly compared to pubertal groups, while, the expression of DNMT3L decreased. These results might be explained by the compensation mechanism among DNMTs, which might be impervious to aging; (3) aging caused increased errors in the distribution and three-dimensional morphology of chromosomes, including the increased total volume and surface area, the high ratio of height to diameter of a circular cylinder enclosing the chromosomes (H/D). Our work provided morphological information for the studies of age-related decline in oocyte qualities.

关键词： DNA甲基转移酶卵母细胞成熟染色体结构小鼠卵母细胞老龄化双光子激光扫描显微镜本地化卵母细胞质量

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中国省域数字经济协同发展研究

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大连海事大学学报（社会科学版） 2023年第2期22卷 56-62页

作者：李东兵马婉昀大连理工大学城市学院管理学院辽宁大连116600 大连海事大学航运经济与管理学院辽宁大连116026

中国省域数字经济协同发展对新发展格局构建和数字经济强国建设有着重要意义。基于复合系统协同度模型,利用2013—2020年中国省域相关数据分析其数字经济协同发展状况,并运用多维正态云模型分析2020年中国省域数字经济水平差距。结果表... 详细信息

中国省域数字经济协同发展对新发展格局构建和数字经济强国建设有着重要意义。基于复合系统协同度模型,利用2013—2020年中国省域相关数据分析其数字经济协同发展状况,并运用多维正态云模型分析2020年中国省域数字经济水平差距。结果表明:中国省域数字经济复合系统整体处于低度协同发展状态,协同化进程较慢但有所提升,未来具有较大上升空间;各省份数字经济发展水平等级跨度大,呈阶梯式不均衡状态。基于此,为促进中国省域数字经济协同发展,应更加关注有效释放不同区域的优势,着力缩小区域间数字经济的发展差距,构建错位发展的数字经济格局,同时高水平数字经济发展地区应当发挥头雁效应,加强制度性交流,寻找合作契机,与中低水平数字经济发展地区共同开拓中国数字经济增长的新空间。

关键词：省域数字经济协同发展区域差异复合系统协同度模型多维正态云模型

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准噶尔盆地乌尔禾地区稀油—稠油—油砂—沥青立体成藏(成矿)模式

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石油学报 2023年第7期44卷 1072-1084页

作者：陈刚强张磊王东勇马万云牛志杰邹贤利中国石油大学(北京)克拉玛依校区石油学院新疆克拉玛依834000 中国石油新疆油田公司勘探开发研究院新疆克拉玛依834000 中国石油大学(北京)油气资源与探测国家重点实验室北京102249 中国石油新疆油田公司实验检测研究院新疆克拉玛依834000

准噶尔盆地乌尔禾地区从埋深5000 m至地表,烃类在多套层系中聚集成藏(成矿)。综合乌尔禾地区构造、沉积、烃类演化和油藏(油矿)的分布特征认为,乌尔禾地区的风城组碱湖相具有优质、成熟和持续生、排烃的源岩条件,在石炭纪之后3期构造运... 详细信息

准噶尔盆地乌尔禾地区从埋深5000 m至地表,烃类在多套层系中聚集成藏(成矿)。综合乌尔禾地区构造、沉积、烃类演化和油藏(油矿)的分布特征认为,乌尔禾地区的风城组碱湖相具有优质、成熟和持续生、排烃的源岩条件,在石炭纪之后3期构造运动控制下形成了多套储-盖组合,其继承性鼻状隆起中的深、浅层"Y"形断裂系统构成了油气的接力输导和圈闭条件,而浅层油气散失、生物降解和氧化作用构成了油气藏的破坏条件。这些造就了乌尔禾地区烃类3期成藏、3期破坏的特征,形成了由二叠系和三叠系稀油油藏、侏罗系稠油油藏、白垩系油砂矿和沥青矿脉构成的稀油—稠油—油砂—沥青立体成藏(成矿)模式。该成藏模式独特,对研究油气多期成藏、烃类演化、断裂对油气成藏的双重作用具有重要意义,对准噶尔盆地西北缘的油气勘探具有指导意义。

关键词：乌尔禾地区稠油油砂矿沥青矿成藏模式

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Melatonin alters the secondary metabolite profile of grape berry skin by promoting VvMYB14-mediated ethylene biosynthesis

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Horticulture Research 2021年第1期8卷 628-642页

作者： wanyun ma Lili Xu Shiwei Gao Xingning Lyu Xiaolei Cao Yuxin Yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China

The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely ***,widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry *** them,27 significantly differentially accumulated metabolites(DAMs)were produced in response to melatonin treatment in the presence or absence of *** of the DAMs were secondary metabolites,including flavonoids,phenolic acids,stilbenes,and ***,the accumulation of 25 DAMs was regulated by melatonin via ***-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ***-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1,VvF3H,VvLAR2,and VvDFR genes,suggesting that these genes may play key roles in regulating secondary metabolites in the skin;additionally,VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary *** experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1,thereby affecting the accumulation of secondary ***,melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14.

关键词： thereby phenolic metabolism

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断层封闭性演化地球化学评价方法及其控藏作用——以准噶尔盆地西北缘红车断裂带为例

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天然气工业 2023年第8期43卷 12-25页

作者：李勇罗力元王剑刘向君马万云陈世加何清波油气藏地质及开发工程全国重点实验室·西南石油大学天然气地质四川省重点实验室西南石油大学地球科学与技术学院中国石油新疆油田公司实验检测研究院

断层封闭性评价对油气成藏研究具有重要的指导意义,但是断层封闭性演化和油气动态成藏有机结合的研究却较少。为此,以准噶尔盆地西北缘红车断裂带为例,利用烃源岩生物标志化合物、生烃热模拟产物特征、原油色谱色质、碳同位素、轻烃等... 详细信息

断层封闭性评价对油气成藏研究具有重要的指导意义,但是断层封闭性演化和油气动态成藏有机结合的研究却较少。为此,以准噶尔盆地西北缘红车断裂带为例,利用烃源岩生物标志化合物、生烃热模拟产物特征、原油色谱色质、碳同位素、轻烃等地球化学实验分析技术,建立了断层封闭性演化地球化学评价方法,揭示了不同地质历史时期断层封闭性差异演化对油气运聚成藏的控制作用。研究结果表明:①红车断裂带断层侧向封闭性控制了断层上/下盘原油性质差异。②晚三叠世—早侏罗世,断层侧向对油气不封闭,下二叠统风城组烃源岩成熟阶段原油运移至上盘石炭系遭受生物降解形成高密度重质油;晚侏罗世—早白垩世,断层侧向对油封闭,对气不封闭,风城组高成熟原油和中二叠统下乌尔禾组成熟原油在下盘聚集成藏。③不同地质历史时期断层对不同流体封闭性差异是红车断裂带油藏伴生气普遍为干气的主要原因。④断层封闭性演化可以划分为开启、弱封闭、中封闭、强封闭4个演化阶段,分别控制了油气散失、上盘油藏遭受生物降解、下盘油藏聚集天然气散失、晚期干气捕获4个成藏过程,前3个阶段导致成熟—高成熟阶段天然气散失,晚期强封闭阶段干气大规模聚集,形成了重质油、中质油、轻质油、干气并存的油气分布复杂现象。结论认为,建立的断裂封闭性动态演化历程的地球化学评价方法,对复杂地区多源多期油气勘探开发具有重要的指导意义。

关键词：断层封闭性地球化学评价控藏作用准噶尔盆地红车断裂带

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准噶尔盆地沙湾凹陷周缘中、浅层天然气地球化学特征及成因

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石油与天然气地质 2022年第1期43卷 175-185页

作者：李二庭靳军王剑马万云陈世加刘翠敏王海静中国石油天然气集团有限公司砾岩油气藏勘探开发重点实验室新疆克拉玛依834000 中国石油新疆油田分公司实验检测研究院新疆克拉玛依834000 西南石油大学资源与环境学院四川成都610500

沙湾凹陷周缘天然气混源现象普遍,前期缺少对地区的整体研究,制约了研究区天然气成藏研究。为此,系统开展了天然气地球化学特征分析,结合烃源岩热模拟技术,明确研究区中、浅层天然气的成因。研究显示,沙湾凹陷周缘中、浅层天然气以甲烷... 详细信息

沙湾凹陷周缘天然气混源现象普遍,前期缺少对地区的整体研究,制约了研究区天然气成藏研究。为此,系统开展了天然气地球化学特征分析,结合烃源岩热模拟技术,明确研究区中、浅层天然气的成因。研究显示,沙湾凹陷周缘中、浅层天然气以甲烷为主,干燥系数分布在0.73~1.00,δ^(13)C_(1)值分布在-56.0‰~-31.5‰,反映研究区成熟与高-过成熟天然气共存;δ^(13)C_(2)值分布在-30.4‰~-22.8‰,反映研究区煤型气、油型气和混合型气均有分布。结合烃源岩热解气碳同位素特征,认为研究区天然气具有4种成因类型:Ⅰ类天然气来源于佳木河组烃源岩,主要分布在红车断裂带中段白垩系,具有极重的δ^(13)C_(2)值,大于-25.5‰,C_(7)轻烃中甲基环己烷含量大于50%;Ⅱ类天然气分布少,主要为原油降解次生生物气,具有异常偏负δ^(13)C_(1)值和极高的干燥系数;Ⅲ类天然气来源于下乌尔禾组烃源岩,主要分布在小拐地区及红车断裂带南段侏罗系,δ^(13)C_(2)值分布在-27.9‰~-26.4‰,具有混合型烃源岩特征;Ⅳ类天然气为下乌尔禾组烃源岩与风城组烃源岩混源,主要分布在红车断裂带南段、北段及金龙地区,以下乌尔禾组来源为主的天然气δ^(13)C_(2)值大于-29‰,以风城组来源为主的天然气δ^(13)C_(2)值小于-29‰。

关键词：碳同位素热模拟油型气煤型气生物降解气天然气沙湾凹陷准噶尔盆地

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