检索结果-南通市图书馆

In vitro antioxidant and wound healing activity of Sargassum polycystum hydroethanolic extract in fibroblasts and keratinocytes

引用

Asian Pacific Journal of Tropical Biomedicine 2023年第5期13卷 222-232页

作者： wanwipha woonnoi Furoida Moolsap Supita Tanasawet Nattakanwadee Khumpirapang Chakkapat Aenglong Wanida Sukketsiri Division of Health and Applied Sciences Faculty of SciencePrince of Songkla UniversitySongkhla 90110Thailand Laboratory Animal Service Center Faculty of SciencePrince of Songkla UniversitySongkhla 90110Thailand Department of Pharmaceutical Chemistry and Pharmacognosy Faculty of Pharmaceutical SciencesNaresuan UniversityPhitsanulok65000Thailand

Objective:To investigate the in vitro antioxidant and wound healing properties of the hydroethanolic extract of Sargassum polycystum,and elucidate the mechanism of its wound healing ***:Human dermal fibroblast and HaCaT cells were used to evaluate the proliferation by sulforhodamine B and dsDNA assay after treatment with Sargassum polycystum *** wound healing and phalloidin-rhodamine staining were employed to observe migratory activity and filopodia formation,*** blot and real-time RT-PCR assays were performed to determine the protein and gene expressions related to wound healing ***:The phytochemical analysis found a higher level of flavonoid than phenolic compound in Sargassum polycystum *** human dermal fibroblast cells,Sargassum polycystum extracts at 50 and 100μg/mL significantly increased fibroblast proliferation and the gene expressions of hyaluronic acid synthase 1(HAS1),HAS2,HAS3,collagen type 1 alpha 1 chain(COL1A1),collagen type 3 alpha 1 chain(COL3A1),and *** phosphorylation of Akt,ERK1/2,and p38 MAPK was also significantly upregulated after treatment with Sargassum polycystum ***,50 and 100μg/mL of the extracts prominently enhanced the proliferation,migration,and filopodia formation of HaCaT cells,as well as the protein levels of pFAK/FAK,pSrc/Src,pAkt/Akt,pERK1/2/ERK1/2,Rac1 and ***:Sargassum polycystum extracts show promising wound healing activities in human dermal fibroblasts and keratinocytes.

关键词： Dermal fibroblast Sargassum polycystum Wound healing Antioxidant Proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：