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Mechanisms of Exogenous Nitric Oxide and 24-Epibrassinolide Alleviating Chlorosis of Peanut Plants Under Iron Deficiency

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Pedosphere 2018年第6期28卷 926-942页

作者： SONG Yiling DONG Yuanjie TIAN Xianyi WANG wanwan Zhenli HE College of Resources and Environment National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Shandong Agricultural University Indian River Research and Education Center Institute of Food and Agricultural Sciences University of Florida

Iron(Fe) is a crucial transition metal for all living organisms including plants; however, Fe deficiency frequently occurs in plant because only a small portion of Fe is bioavailable in soil in recent years. To cope with Fe deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiology. To understand the role of nitric oxide(NO) and 24-epibrassinolide(EBR) in alleviating chlorosis induced by Fe deficiency in peanut(Arachis hypogaea L.) plants, we determined the concentration of chlorophylls, the activation, uptake, and translocation of Fe, the activities of key enzymes, such as ferric-chelate reductase(FCR),proton-translocating adenosine triphosphatase(H^+-ATPase), and antioxidant enzymes, and the accumulation of reactive oxygen species(ROS) and malondialdehyde(MDA) of peanut plants under Fe sufficiency(100 μmol L^(-1)ethylenediaminetetraacetic acid(EDTA)-Fe) and Fe deficiency(0 μmol L^(-1)EDTA-Fe). We also investigated the production of NO in peanut plants subjected to Fe deficiency with foliar application of sodium nitroprusside(SNP), a donor of NO, and/or EBR. The results showed that Fe deficiency resulted in severe chlorosis and oxidative stress, significantly decreased the concentration of chlorophylls and active Fe, and significantly increased NO production. Foliar application of NO and/or EBR increased the activity of antioxidant enzymes, superoxide dismutase,peroxidase, and catalase, and decreased the ROS and MDA concentrations, thus enhancing the resistance of plants to oxidative *** of NO also significantly increased Fe translocation from the roots to the shoots and enhanced the transfer of Fe from the cell wall fraction to the cell organelle and soluble fractions. Consequently, the concentrations of available Fe and chlorophylls in the leaves were elevated. Furthermore, the activities of H^+-ATPase and FCR were enhanced in the Fe-deficient plants. Simultaneously,there was a significant in

关键词： active iron antioxidant enzyme subcellular structure chlorophyll oxidative stress reactive oxygen species sodium nitroprusside

Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastids

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journal of Integrative Plant Biology 2023年第4期65卷 1003-1011页

作者： Wenbo Xu Miao Zhang Yangcun Li wanwan He Shengchun Li j.ang Zhang State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei Hongshan LaboratorySchool of Life SciencesHubei UniversityWuhan 430062China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518000China

RNA interference(RNAi)has emerged as a powerful technology for pest ***,we have shown that plastid-mediated RNAi(PM-RNAi)can be utilized to control the Colorado potato beetle,an insect pest in the Chrysomelidae family;however,whether this technology is suitable for controlling pests in the Coccinellidae remained *** coccinellid 28-spotted potato ladybird(Henosepilachna vigintioctopunctata;HV)is a serious pest of solanaceous *** this study,we identified three efficient target genes(β-Actin,SRP54,and SNAP)for RNAi using in vitro double-stranded RNAs(dsRNAs)fed to HV,and found that dsRNAs targetingβ-Actin messenger RNA(dsACT)induced more potent RNAi than those targeting the other two *** next generated transplastomic and nuclear transgenic potato(Solanum tuberosum)plants expressing HV *** dsACT stably accumulated to up to 0.7%of the total cellular RNA in the transplastomic plants,at least three orders of magnitude higher than in the nuclear transgenic ***,the transplastomic plants also exhibited a significantly stronger resistance to HV,killing all larvae within 6 *** data demonstrate the potential of PM-RNAi as an efficient pest control measure for HV,extending the application range of this technology to Coccinellidae pests.

关键词： double‐stranded RNA Henosepilachna vigintioctopunctata pest control plastid transformation RNA interference

Cystathionine-γ-lyase ameliorates the histone demethylase jMjD3-mediated autoimmune response in rheumatoid arthritis

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Cellular & Molecular Immunology 2019年第8期16卷 694-705页

作者： Weijun Wu Ming Qin wanwan j.a Zheng Huang Zhongzheng Li Di Yang Mengwei Huang Chenxi Xiao Fen Long j.anchun Mao Philip K.Moore Xinhua Liu Yi Zhun Zhu Department of Pharmacology Shanghai Key Laboratory of Bioactive Small MoleculesSchool of PharmacyFudan UniversityShanghai 201203China State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy Macao University of Science and TechnologyMacaoChina Institute of Arthritis Research Shanghai Academy of Chinese Medical SciencesGuanghua Integrative Medicine Hospital/Shanghai University of T.C.MShanghaiChina Ningbo The 9th Hospital Ningbo 315000China Department of Rheumatology Shanghai Longhua HospitalShanghaiChina Department of Pharmacology National University of SingaporeSingaporeSingapore

Cystathionine-γ-lyase(CSE),an enzyme associated with hydrogen sulfide(H2S)production,is an important endogenous regulator of *** domain-containing protein 3(jMjD3)is implicated in the immune response and ***,we investigated the potential contribution of jMjD3 to endogenous CSE-mediated inflammation in rheumatoid arthritis(RA).Upregulated CSE and jMjD3 were identified in synovial fibroblasts(SFs)from RA patients as well as in the joints of arthritic *** down CSE augmented inflammation in IL-1β-induced SFs by increasing jMjD3 *** addition,CSE−/−mice with collagen-induced arthritis(CIA)developed severe joint inflammation and bone ***,overexpressing CSE inhibited jMjD3 expression by the transcription factor Sp-1 and was accompanied by reduced inflammation in IL-1β-treated ***,jMjD3 silencing or the administration of the jMjD3 inhibitor GSK-j4 significantly decreased the inflammatory response in IL-1β-treated SFs,mainly by controlling the methylation status of H3K27me3 at the promoter of its target ***-j4 markedly attenuated the severity of arthritis in CIA *** conclusion,suppressing jMjD3 expression by the transcription factor Sp-1 is likely responsible for the ability of CSE to negatively modulate the inflammatory response and reduce the progression of RA.

关键词： cystathionine-γ-lyase rheumatoid arthritis jumonji domain-containing protein 3 Sp-1 Toll like receptor 2

Anti-wrinkle Finishing of Cotton Fabrics with Pyromellitic Acid Enhanced by Polyol Extenders

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journal of Donghua University(English Edition) 2022年第6期39卷 533-541页

作者： QI Huan MENG Lujie LIN Xiaofang XU wanwan CHEN Yangyi ZHANG Chuyang QIU Yiping College of Textiles and Apparel Quanzhou Normal UniversityQuanzhou 362002China Key Laboratory of Clothing Materials of Universities in Fuj.an(Quanzhou Normal University) Quanzhou 362002China School of Textile Science and Engineering Tiangong UniversityTianjin 300387China

Pyromellitic acid(PMA)has been employed for formaldehyde-free anti-wrinkle finishing on cotton fabrics for its low damage to fibers and absence of phosphorus-containing ***,the wrinkle resistance performance of fabrics failed to meet the application *** this work,the polyol extenders were introduced into PMA cross-linked cellulose for further improving fabric resilience by a two-step dipping-squeezing-curing *** polyols were employed as the chain extenders for PMA anti-wrinkle finishing on fabrics,among which sorbitol presented the best chain-spreading effect on wrinkle *** screening the process parameters,the optimum process was obtained as *** dosage ratio of PMA to sorbitol was 1∶1,the curing condition was 150-160℃ for 2-3 *** electron microscopy(SEM),thermogravimetric analysis(TGA)and Fourier transform infrared(FTIR)spectroscopy were employed to characterize the fiber changes during the *** results showed that the sorbitol incorporation could facilitate the remelting and redissolution of PMA on fiber *** the curing process,sorbitol could form ester linkages between PMA molecules and improve the fiber *** treated samples presented excellent washing durability,and the wrinkle recovery angle A_(w)was still 265.1°after 10 washes,which was close to that of 1,2,3,4-butane tetracarboxylic acid(BTCA)but with lower tearing load *** results provide new insights for the application of PMA in formaldehyde-free anti-wrinkle finishing on cotton fabrics.

关键词： pyromellitic acid polyol extender anti-wrinkle finishing esterification extended crosslinking

SBA-15微球负载Karstedt催化剂制备及对硅氢加成反应动力学

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硅酸盐学报 2016年第3期44卷 419-424页

作者：陈伟闫婉婉胡庆华九江学院化学与环境工程学院江西九江332005 江西省生态化工工程技术研究中心江西九江332005

以三嵌段共聚物P123（EO20PO70EO20）为模板剂、正硅酸乙脂（TEOS）为硅源、氯化氢（HCl）为催化剂,通过水热法制备了介孔Si O2（SBA-15）微球,并以之为载体,制备了SBA-15负载Karstedt催化剂。通过扫描电子显微镜、小角X射线衍射、透射... 详细信息

以三嵌段共聚物P123（EO20PO70EO20）为模板剂、正硅酸乙脂（TEOS）为硅源、氯化氢（HCl）为催化剂,通过水热法制备了介孔Si O2（SBA-15）微球,并以之为载体,制备了SBA-15负载Karstedt催化剂。通过扫描电子显微镜、小角X射线衍射、透射电子显微镜、氮气等温吸附–脱附实验等对催化剂形貌和结构进行表征。结果表明：催化剂直径为2~4μm,该微球具有高度有序二维贯通介孔结构,最可几孔径分布为4.5 nm,比表面积高达628 m2/g。以1-十八烯和三甲氧基硅烷硅氢加成反应为探针反应,研究了不同温度下催化剂的催化性能。当催化剂用量为反应物质量的5%、反应温度为80℃、反应时间为5 h时,1-十八烯转化率可达67.2%,反应活化能为9.176 k j/mol。

关键词： SBA-15微球硅氢加成反应 Karstedt催化剂动力学

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中医土形人之亚型内涵解析

中华中医药杂志 2024年第1期39卷 410-412页

作者：李佳佳贾竑晓 jing-Dong j.Han 杨心宇崔静雯首都医科大学附属安定医院国家精神心理疾病临床研究中心精神疾病诊断与治疗北京市重点实验室北京100088 人脑保护高精尖创新中心首都医科大学北京100069 北京大学前沿交叉学科研究院北大-清华生命科学联合中心定量生物学中心北京100871 北京大学生命科学学院北京100871

《灵枢·阴阳二十五人》根据五行理论,构建了包含25种亚型的形神分类模型。亚型是中医五形人理论模型的具体演绎与表达,是打开中医五形人理论模型的钥匙,具有重要的研究意义。根据中医五形人理论,中医土形人可再分为5种亚型,分别为敦敦... 详细信息

《灵枢·阴阳二十五人》根据五行理论,构建了包含25种亚型的形神分类模型。亚型是中医五形人理论模型的具体演绎与表达,是打开中医五形人理论模型的钥匙,具有重要的研究意义。根据中医五形人理论,中医土形人可再分为5种亚型,分别为敦敦然、婉婉然、坎坎然、枢枢然、兀兀然。原文行文简练,内涵丰富,但是目前相关研究较少。本研究试图基于文字学、文献学、理论推导及对3D人脸的样本观察,解析中医土形人亚型的内涵,以更好地了解中医土形人及亚型的形神特征,更好地指导中医土行人及亚型的临床辨证。

关键词：中医五形人中医土形人阴阳二十五人亚型土行特质能量五行互藏经脉气血盛衰特征

含Co不锈钢析出相的模拟研究与强韧性能变化

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金属热处理 2020年第7期45卷 143-147页

作者：陈婉婉邹德宁李姣张英波李雨浓西安建筑科技大学冶金工程学院陕西西安710055

采用数值模拟、组织表征和性能测试等方法,研究了添加Co对马氏体时效硬化不锈钢微观组织和力学性能的影响。根据Thermo-Calc热力学及动力学计算结果:6wt%Co条件下,ε-Cu相的析出温度升高且孕育时间变长,相同时效条件下ε-Cu相的平均半... 详细信息

采用数值模拟、组织表征和性能测试等方法,研究了添加Co对马氏体时效硬化不锈钢微观组织和力学性能的影响。根据Thermo-Calc热力学及动力学计算结果:6wt%Co条件下,ε-Cu相的析出温度升高且孕育时间变长,相同时效条件下ε-Cu相的平均半径会逐渐减小。借助光学显微镜(OM)及透射电镜(TEM)分析发现,固溶+时效处理后含Co试验钢超低碳板条马氏体细化,弥散分布的ε-Cu相尺寸变小且析出量增加,薄膜状逆变奥氏体数量增多且尺寸增大。由于板条马氏体的位错强化、ε-Cu相的析出强化以及逆变奥氏体相的增韧作用,含Co试验钢的抗拉强度、硬度、伸长率及冲击吸收能量分别达到1346 MPa、433 HV0.5、16%和73 j。

关键词：马氏体时效硬化不锈钢钴合金化 Thermo-Calc软件 ε-Cu相

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柔性相变材料对铅酸电池组的高低温性能强化

低温与超导 2023年第9期51卷 79-84页

作者：郑凯轩李皖皖刘民安蔡永爽郑州工业应用技术学院建筑工程学院郑州451100 河南工业大学土木工程学院郑州450001 中国人民解放军93032部队延吉133000

针对铅酸电池组在高低温环境下性能衰减的问题,制备出相变温度为39.6℃,相变潜热为143.5 j/g的柔性相变材料(PCM),搭建了相变热管理系统,研究PCM对电池组的高低温充放电特性的影响。在-10℃充放电时,PCM可大幅提升电池组的温度,将部分... 详细信息

针对铅酸电池组在高低温环境下性能衰减的问题,制备出相变温度为39.6℃,相变潜热为143.5 j/g的柔性相变材料(PCM),搭建了相变热管理系统,研究PCM对电池组的高低温充放电特性的影响。在-10℃充放电时,PCM可大幅提升电池组的温度,将部分测点温度提升至0℃以上。在40℃高温下,PCM可在充放电过程中显著降低电池组的温度,温度降幅最高为2.9℃。结果表明:PCM可降低高低温下电池的过放过充容量,柔性相变材料可显著提升电池组在高低温环境中的热特性和电特性。

关键词：铅酸电池组相变热管理高低温工况性能提升