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Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization

The Crop Journal 2021年第1期9卷 95-108页

作者： Jingxin Wang Wenbo Pan Alexiy Nikiforov William King wanting hong Weiwei Li Yang Han Jana Patton-Vogt Jianbo Shen Lingyun Cheng Key Laboratory of Plant-Soil Interactions Ministry of Education/Key Laboratory of Plant NutritionMinistry of AgricultureDepartment of Plant NutritionChina Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Department of Biological Sciences Duquesne UniversityPittsburghPA 15282USA

Phosphate deficiency is one of the leading causes of crop productivity *** degradation liberates phosphate to cope with phosphate *** phosphodiesterases(GPX-PDEs)hydrolyse the intermediate products of phospholipid catabolism glycerophosphodiesters into glycerol-3-phosphate,a precursor of ***,the function of GPX-PDEs in phosphate remobilization in maize remains *** the present study,we characterized two phosphate deficiency-inducible GPX-PDE genes,ZmGPX-PDE1 and ZmGPX-PDE5,in maize ***-PDE1 and ZmGPX-PDE5 were transcriptionally regulated by ZmPHR1,a well-described phosphate starvation-responsive transcription factor of the MYB *** of the yeast GPX-PDE mutant gde1Δindicated that ZmGPX-PDE1 and ZmGPX-PDE5 functioned as GPX-PDEs,suggesting their roles in phosphate recycling from *** vitro enzyme assays showed that ZmGPX-PDE1 and ZmGPX-PDE5 catalysed glycerophosphodiester degradation with different substrate preferences for glycerophosphoinositol and glycerophosphocholine,***-PDE1 was upregulated during leaf senescence,and more remarkably,loss of ZmGPXPDE1 inmaize compromised the remobilization of phosphorus fromsenescing leaves to young leaves,resulting in a stay-green phenotype under phosphate *** results suggest that ZmGPX-PDE1 catalyses the degradation of glycerophosphodiesters in maize,promoting phosphate recycling from senescing leaves to new *** mechanism is crucial for improving phosphorus utilization efficiency in crops.

关键词： Glycerophosphodiester phosphodiesterases Maize Phosphate deficiency Phosphorus remobilization Phospholipid

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Extremum of a time-inhomogeneous branching random walk

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Frontiers of Mathematics in China 2021年第2期16卷 459-478页

作者： wanting HOU Xiaoyue ZHANG Wenming hong Department of Mathematics Northeastern UniversityShenyang 110004China School of Statistics Capital University of Economics and BusinessBeijing 100070China School of Mathematical Sciences&Laboratory of Mathematics and Complex Systems Beijing Normal UniversityBeijing 100875China

Consider a time-inhomogeneous branching random walk, generated by the point process Ln which composed by two independent parts: ‘branching’offspring Xn with the mean 1+B(1+n)−β for β∈(0,1) and ‘displacement’ ξn with a drift A(1+n)^(−2α) for α∈(0,1/2), where the ‘branching’ process is supercritical for B>0 but ‘asymptotically critical’ and the drift of the ‘displacement’ ξn is strictly positive or negative for |A|>0 but ‘asymptotically’ goes to zero as time goes to infinity. We find that the limit behavior of the minimal (or maximal) position of the branching random walk is sensitive to the ‘asymptotical’ parameter β and α.

关键词： Branching random walk time-inhomogeneous branching process random walk

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人因视角融入的景观认知研究进展及展望

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风景园林 2022年第6期29卷 63-69页

作者：解雨歌洪婉婷 [意大利]马特奥·波利李方正北京林业大学园林学院米兰理工大学建筑与城市研究系

人的景观认知对于城市绿色空间优化具有重要价值,然而目前此类研究仍由主观认知经验主导,缺少客观测度方法支撑。引入人因工程学视角,界定人因耦合系统下的景观认知对象,总结人因场景构建及测度技术方法,梳理人因耦合的景观认知相关研究... 详细信息

人的景观认知对于城市绿色空间优化具有重要价值,然而目前此类研究仍由主观认知经验主导,缺少客观测度方法支撑。引入人因工程学视角,界定人因耦合系统下的景观认知对象,总结人因场景构建及测度技术方法,梳理人因耦合的景观认知相关研究,归纳出三大类研究内容:1)景观认知产生机制;2)景观认知主体评价;3)景观认知行为效应。从城市-社区-场地三重维度解析人因视角融合下的景观认知研究发展趋势,阐明开展此类研究的重要价值。

关键词：风景园林人因工程学城市绿色空间景观认知认知主体人本主义

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当归补血汤基准样品质量控制方法研究

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药物评价研究 2022年第11期45卷 2247-2256页

作者：陈锦霞高永坚林碧珊陈伟钢洪婉婷张汝莹陈文慧梁浩明国药集团广东环球制药有限公司广东佛山528303

目的建立经典名方当归补血汤基准样品系统的质量控制方法,为其质量评价提供参考。方法制备26批基准样品,在同一供试品溶液下,基于超高效液相色谱-二极管阵列检测器(UPLC-PDA)、UPLC-蒸发光散射检测器(ELSD)、亲水相互作用色谱-二极管... 详细信息

目的建立经典名方当归补血汤基准样品系统的质量控制方法,为其质量评价提供参考。方法制备26批基准样品,在同一供试品溶液下,基于超高效液相色谱-二极管阵列检测器(UPLC-PDA)、UPLC-蒸发光散射检测器(ELSD)、亲水相互作用色谱-二极管阵列检测器(HILIC-PDA)法建立当归补血汤基准样品多维指纹图谱,明确其指纹图谱的峰归属和相似度范围。对毛蕊异黄酮葡萄糖苷和毛蕊异黄酮、黄芪甲苷、阿魏酸、总多糖进行定量分析,确定其含量限度范围。结果建立当归补血汤基准样品三维指纹图谱方法,共标定了32个共有峰,通过与对照品比对,指认了其中17个共有峰,使用“中药色谱指纹图谱相似度评价系统(2012版)”计算相似度,其相似度均大于0.9。26批基准样品毛蕊异黄酮葡萄糖苷和毛蕊异黄酮、黄芪甲苷、阿魏酸、总多糖的含量均值±30%范围分别为0.075%~0.140%、0.023%~0.043%、0.103%~0.191%、4.69%~8.70%。结论建立经典名方当归补血汤多维指纹图谱及多成分含量测定方法,适用性强,重复性、稳定性良好,可为当归补血汤基准样品及其制剂的质量控制提供依据。

关键词：经典名方当归补血汤基准样品超高效液相色谱(UPLC) 指纹图谱毛蕊异黄酮葡萄糖苷毛蕊异黄酮黄芪甲苷阿魏酸

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抗阻呼吸训练对慢性心力衰竭患者的心肺功能及焦虑情绪的影响

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医学临床研究 2024年第2期41卷 181-184页

作者：杨南华杨胜勇洪婉婷霍欣李冬来管晓波陈超上海市第二康复医院心脏康复科上海200431 上海市民政第三精神卫生中心精神科上海200065

【目的】探讨抗阻呼吸训练对慢性心力衰竭(CHF)患者的心肺功能及焦虑情绪的影响。【方法】128例CHF稳定期患者随机分为观察组和对照组,每组64例。对照组患者给予CHF常规药物治疗8周,观察组在对照组治疗的基础上联合使用呼吸抗阻训练。... 详细信息

【目的】探讨抗阻呼吸训练对慢性心力衰竭(CHF)患者的心肺功能及焦虑情绪的影响。【方法】128例CHF稳定期患者随机分为观察组和对照组,每组64例。对照组患者给予CHF常规药物治疗8周,观察组在对照组治疗的基础上联合使用呼吸抗阻训练。患者干预前后分别进行肺功能[第1秒用力呼气容积(FEV 1)、FEV 1/用力肺活量(FEV 1/FVC%)]、6 min步行试验(6MWT)、呼吸困难量表(mMRC)、广泛性焦虑障碍量表[(GAD)-7]的测试,比较两组患者心肺功能、焦虑情绪状况及不良应发生情况。【结果】干预后,两组FEV 1、FEV 1/FVC均较干预前升高(P0.05);观察组mMRC分级优于对照组(P0.05),且两组干预后6MWT分级比较,差异无统计学意义(P>0.05);两组GAD-7评分均较干预前降低(P0.05)。【结论】呼吸抗阻训练联合药物治疗能有效改善CHF患者的呼吸功能及肺通气功能,缓解患者负性情绪,为促进患者的康复提供了新治疗途径。

关键词：心力衰竭/康复慢性病呼吸锻炼心脏功能试验焦虑

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Advances in understanding the roles of actin scaffolding and membrane trafficking in dendrite development

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Journal of Genetics and Genomics 2024年第11期51卷 1151-1161页

作者： wanting Wang Menglong Rui School of Life Science and Technology The Key Laboratory of Developmental Genes and Human DiseaseSoutheast UniversityNanjingJiangsu 210031China

Dendritic morphology is typically highly branched,and the branching and synaptic abundance of dendrites can enhance the receptive range of neurons and the diversity of information received,thus providing the basis for information processing in the nervous *** dendritic development is aberrantly compromised or damaged,it may lead to abnormal connectivity of the neural network,affecting the function and stability of the nervous system and ultimately triggering a series of neurological *** on the regulation of dendritic developmental processes has flourished,and much progress is now being made in its regulatory ***,dendrites are characterized by an extremely complex dendritic arborization that cannot be attributed to individual protein functions alone,requiring a systematic analysis of the intrinsic and extrinsic signals and the coordinated roles among *** cytoskeleton organization and membrane vesicle trafficking are required during dendrite development,with actin providing tracks for vesicles and vesicle trafficking in turn providing material for actin *** this review,we focus on these two basic biological processes and discuss the molecular mechanisms and their synergistic effects underlying the morphogenesis of neuronal *** also offer insights and discuss strategies for the potential preventive and therapeutic treatment of neuropsychiatric disorders.

关键词： Actin Membrane vesicle transport Exocyst complex Secretory pathway Dendrite development Neurological disease

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Study on the Formation and Separation Process of Droplets in the Medical Piezoelectric Atomization Device Induced by Intra‑hole Fluctuation

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Chinese Journal of Mechanical Engineering 2022年第3期35卷 285-296页

作者： Qiufeng Yan wanting Sun Jianhui Zhang School of Electrical Engineering Nantong UniversityNantong 226019China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China College of Mechanical and Electrical Engineering Guangzhou UniversityGuangzhou 510006China

Traditional atomization devices always exhibit many drawbacks,such as non-uniform atomization particle sizes,instability of transient atomization quantity and uncontrollability of precise energy,which seriously restrict further practical application of atomization inhalation *** formation and separation process of droplets belongs to a microphenomenon of *** investigation of the droplet formation and separation process will be favorable for understanding the atomization *** present work,the Conservative Level Set Method(CLSM)is successfully applied on the simulation of the formation and separation of droplets in a medical piezoelectric atomization device induced by intra-hole *** intra-hole fluctuation mechanism is systematically explored and analyzed,and also the expression of the volume change in the micro cone hole is built and *** the control equation and simulation model of droplet formation and separation process have been well established by meshing the simulation model,and thereby the process of droplet formation and separation is *** corresponding results demonstrate that the breaking time of droplets is decreased with the inlet velocity and liquid temperature rising,while enhanced with the liquid concentration ***,the volume of droplet is decreased with the inlet velocity and liquid concentration increasing,but increased with the liquid temperature *** velocity of droplet is enhanced with the inlet velocity and liquid temperature rising,and reduced with the increase of liquid *** the large side diameter of micro-cone hole is set as 79μm,the breaking time of the droplet reaches a minimum value of 38.7μs,whereas the volume and the velocity of droplet reach a maximum value of 79.8 pL and 4.46 m/s,*** study provides theoretical guidance for the design of medical piezoelectric atomization devices and contributes to the promotion of inhalation thera

关键词： CLSM Intra-hole fluctuation Piezoelectric atomization Droplet distribution

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A RISK-AVERSE NEWSVENDOR MODEL WITH LIMITED CAPACITY AND OUTSOURCING UNDER THE CVAR CRITERION

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Journal of Systems Science and Systems Engineering 2015年第1期24卷 49-67页

作者： Zhenwei Luo Jinting Wang wanting Chen Department of Mathematics School of ScienceBeijing Jiaotong University

For seasonal products, the retailers usually launch various marketing ettorts, llKe aaverusmg tmu promotion, to promote them in a selling season. While facing large demand from the customers, one should take the capacity constraint and outsotlrcing into consideration. Considering the shorten life cycles of most products, in this paper we adopt the traditional newsvendor model to investigate the optimal marketing effort along with optimal order quantity. We address the risk aversion issue and characterize the influence of the sellers＇ risk propensity with CVaR criterion, and we develop an effective algorithm to obtain the optimal strategy. The impact of sellers＇ risk propensity on the performance of the system is illustrated via numerical examples, The innovation of this paper is threefold. First, the optimal joint strategy of the marketing effort and order quantity is investigated and an efficient algorithm to find the optimal strategy is developed. Second, the capacity constraint option and an outsourcing strategy are studied jointly for excess products. Finally, the risk propensity of the seller and its influence are investigated by using the CVaR criterion, through which we obtain some new managerial insights.

关键词： Newsvendor model marketing effort order quantity CVaR capacity constraint,outsourcing

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Dynamic Stiffness Analysis and Experimental Verification of Axial Magnetic Bearing Based on Air Gap Flux Variation in Magnetically Suspended Molecular Pump

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Chinese Journal of Mechanical Engineering 2020年第4期33卷 139-149页

作者： Jinji Sun wanting Wei Jiqiang Tang Chun‑E Wang Key Laboratory of Fundamental Science for National Defense Novel Inertial Instrument and Navigation System TechnologySchool of Instrument Science and Opto‑Electronics EngineeringBeihang UniversityBeijing 100191China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001China Beijing Institute of Automatic Control Equipment Beijing 100074China

Current and displacement stiffness are important parameters of axial magnetic bearing(AMB)and are usually considered as constants for the control ***,in actual dynamic work situations,time-varying force leads to time-varying currents and air gap with a specific frequency,which makes the stiffness of appear decrease and even worsens control performance for the whole *** this paper,an AMB dynamic stiffness model considering the flux variation across the air gap due to frequency is established to obtain the accurate dynamic *** dynamic stiffness characteristics are analyzed by means of the dynamic equivalent magnetic circuit *** analytical results show that the amplitude of current and displacement stiffness decreases with frequency ***,compared with the stiffness model without considering the variation of flux density across the air gap,the improved dynamic stiffness results are closer to the actual *** the dynamic stiffness measurement method of AMB,experiments of AMB in magnetically suspended molecular pump(MSMP)are carried out and the experimental results are consistent with theoretical analysis *** paper proposes the dynamic stiffness model of axial magnetic bearing considering the variation of flux density across the air gap,which improves the accuracy of the AMB stiffness analysis.

关键词： Axial magnetic bearing(AMB) Dynamic stiffness Magnetically suspended molecular pump(MSMP)

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脑卒中后恢复期患者抑郁影响因素和筛查效果分析

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临床荟萃 2020年第12期35卷 1101-1105页

作者：洪婉婷孙秀丽鲍兰军杨南华陈骕管晓波施敏上海市第二康复医院老年康复科上海201900 上海市民政第三精神卫生中心康复三病区上海200435

目的探讨脑卒中后恢复期患者抑郁情况的分布及影响因素和抑郁自评量表量表对抑郁障碍的筛查效果。方法纳入脑卒中后恢复期患者43例,采集人口统计学、心率、高血压病、BMI、糖化血红蛋白、血胆固醇、血甘油三酯、凝血因子活性结果,予焦... 详细信息

目的探讨脑卒中后恢复期患者抑郁情况的分布及影响因素和抑郁自评量表量表对抑郁障碍的筛查效果。方法纳入脑卒中后恢复期患者43例,采集人口统计学、心率、高血压病、BMI、糖化血红蛋白、血胆固醇、血甘油三酯、凝血因子活性结果,予焦虑自评量表(SAS)、抑郁自评量表(SDS)、匹兹堡睡眠指数量表(PSQI)测量患者的焦虑、抑郁和睡眠质量情况,建立回归模型观察纳入变量对SDS量表评分的影响。结果43例中评分指数达到抑郁者14例(32.6%)。回归分析显示发现,HbA1c(β=4.01,P<0.01)与PSQI(β=0.83,P<0.05)对SDS量表评分有显著性影响,且HbA1c回归系数较大。ROC曲线诊断发现ROC曲线下面积AUC为0.841(95%CI:0.713-0.969,P<0.01),最佳界值40.5,Youden指数0.592,敏感性69.2%,特异性90%。结论临床上应对脑卒中后高血糖、睡眠质量差者进行及时筛查和干预。SDS量表能有效筛查患者的抑郁障碍。

关键词：脑血管意外抑郁康复因素分析,统计学

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