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Horticultural Plant Journal 2023年第3期9卷 425-436页

作者： Wenhui Yang Jiaqin Ren wanrong liu Dan liu Kaidong Xie Fei Zhang Pengwei Wang Wenwu Guo Xiaomeng Wu National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan Laboratory WuhanHubei 430070China

Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for gene functional characterization and CRISPR/Cas9 genome editing in higher plants, but it has not been widely used in citrus. In this study, the polyethylene glycol(PEG)-mediated method was optimized for citrus callus protoplast transfection, with an improved transfection efficiency of 68.4%. Consequently, the efficiency of protein subcellular localization assay was increased to 65.8%, through transient expression of the target gene in protoplasts that stably express the fluorescent organelle marker protein. The gene editing frequencies in citrus callus protoplasts reached 14.2% after transient expression of CRISPR/Cas9 constructs. We demonstrated that the intronic polycistronic tRNAgRNA(inPTG) genome editing construct was functional in both the protoplast transient expression system and epicotyl stable transformation system in citrus. With this optimized protoplast transient expression system, we improved the efficiency of protein subcellular localization assay and developed the genome editing system in callus protoplasts, which provides an approach for prompt test of CRISPR vectors.

关键词： Citrus Callus protoplast Transient transfection Subcellular localization Genome editing

Flexible multiterminal photoelectronic neurotransistors based on self‐assembled rubber semiconductors for spatiotemporal information processing

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SmartMat 2023年第2期4卷 78-87页

作者： Yunchao Xu Gengming Zhang wanrong liu Chenxing Jin Yiling Nie Jia Sun Junliang Yang Hunan Key Laboratory for Super Microstructure and Ultrafast Process School of Physics and ElectronicsCentral South UniversityChangshaChina Hunan Key Laboratory of Nanophotonics and Devices School of Physics and ElectronicsCentral South UniversityChangshaChina

A significant step toward constructing high‐efficiency neuromorphic systems is the electronic emulation of advanced synaptic functions of the human brain.While previous studies have focused on mimicking the basic functions of synapses using single‐gate transistors,multigate transistors offer an opportunity to simulate more complex and advanced memory‐forming behaviors in biological synapses.In this study,a simple and general method is used to assemble rubber semiconductors into suspended two‐phase composite films that are transferred to the surface of the ion‐conducting membrane to fabricate flexible multiterminal photoelectronic neurotransistors.The suspended ion conductive film is used as the gate dielectrics and supporting substrate.The prepared devices exhibit excellent electrical stability and mechanical flexibility after being bent.Basic photoelectronic synaptic behavior and pulse‐dependent plasticity are emulated.Furthermore,the device realizes the spatiotemporally integrated electrical and optical stimuli to mimic spatiotemporal information processing.This study provides a promising direction for constructing more complex spiking neural networks and more powerful neuromorphic systems with brain‐like dynamic spatiotemporal processing functions.

关键词： ion‐conducting membrane multiterminal neuromorphic devices optoelectronic neurotransistors self‐assembly semiconductor spatiotemporal information processing

Hybrid C8-BTBT/In GaAs nanowire heterojunction for artificial photosynaptic transistors

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Journal of Semiconductors 2022年第11期43卷 9-21页

作者： Yiling Nie Pengshan Xie Xu Chen Chenxing Jin wanrong liu Xiaofang Shi Yunchao Xu Yongyi Peng Johnny C.Ho Jia Sun Junliang Yang Hunan Key Laboratory for Super Microstructure and Ultrafast Process School of Physics and ElectronicsCentral South UniversityChangsha 410083China Department of Materials Science and Engineering City University of Hong KongKowloon 999077Hong Kong SARChina State Key Laboratory of Terahertz and Millimeter Waves City University of Hong KongKowloon 999077Hong Kong SARChina Key Laboratory of Advanced Materials Processing&Mold(Zhengzhou University) Ministry of EducationZhengzhou 450002China

The emergence of light-tunable synaptic transistors provides opportunities to break through the von Neumann bottleneck and enable neuromorphic computing.Herein,a multifunctional synaptic transistor is constructed by using 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene(C8-BTBT)and indium gallium arsenide(InGaAs)nanowires(NWs)hybrid heterojunction thin film as the active layer.Under illumination,the Type-I C8-BTBT/InGaAs NWs heterojunction would make the dissociated photogenerated excitons more difficult to recombine.The persistent photoconductivity caused by charge trapping can then be used to mimic photosynaptic behaviors,including excitatory postsynaptic current,long/short-term memory and Pavlovian learning.Furthermore,a high classification accuracy of 89.72%can be achieved through the single-layer-perceptron hardware-based neural network built from C8-BTBT/InGaAs NWs synaptic transistors.Thus,this work could provide new insights into the fabrication of high-performance optoelectronic synaptic devices.

关键词： photonic synaptic transistor C8-BTBT InGaAs heterojunction

Removing and recycling mercury from scrubbing solution produced in wet nonferrous metal smelting flue gas purification process

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Journal of Environmental Sciences 2021年第5期33卷 59-68页

作者： Shu Yang Ziliang Li Kang Yan Xi Zhang Zhifeng Xu wanrong liu Zhilou liu Hui liu School of Metallurgy Engineering JiangXi University of Science and TechnologyGanzhou 341000China School of Metallurgy and Environment Central South UniversityChangsha 410083China Institute of Green Metallurgy and Process Intensification Ganzhou 341000China Solid Waste and Chemicals Management Center Ministry of Environmental ProtectionBeijing 100024China

Wet purification technology for nonferrous metal smelting flue gas is important for mercury removal;however, this technology produces a large amounts of spent scrubbing solution that contain mercury. The mercury in these scrubbing solutions pose a great threat to the environment. Therefore, this research provides a novel strategy for removing and recycling mercury from the scrubbing solution, which is significant for decreasing mercury pollution while also allowing for the safe disposal of wastewater and a stable supply of mercury resources. Some critical parameters for the electrochemical reduction of mercury were studied in detail. Additionally, the electrodeposition dynamics and electroreduction mechanism for mercury were evaluated. Results suggested that over 92.4% of mercury could be removed from the scrubbing solution in the form of a Hg-Cu alloy under optimal conditions within 150 min and with a current efficiency of approximately 75%. Additionally, mercury electrodeposition was a quasi-reversible process, and the controlled step was the mass transport of the reactant. A pre-conversion step from Hg(Tu)_(4)^(2+) to Hg(Tu)_(3)^(2+) before mercury electroreduction was necessary. Then, the formed Hg(Tu)_(3)^(2+) on the cathode surface gained electrons step by step. After electrodeposition, the mercury in the spent cathode could be recycled by thermal desorption. The results of the electrochemical reduction of mercury and subsequent recycling provides a practical and easy-to-adopt alternative for recycling mercury resources and decreasing mercury contamination.

关键词： Mercury Electrodeposition Scrubbing solution Pollution control

Layer-by-layer slot-die coated high-efficiency organic solar cells processed using twin boiling point solvents under ambient condition

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Nano Research 2021年第11期14卷 4236-4242页

作者： Yu Yang Erming Feng Hengyue Li Zichao Shen wanrong liu Jingbo Guo Qun Luo Jidong Zhang Guanghao Lu Changqi Ma Junliang Yang Hunan Key Laboratory for Super-microstructure and Ultrafast Process School of Physics and ElectronicsCentral South UniversityChangsha410083China Frontier Institute of Science and Technology Xi’an Jiaotong UniversityXi’an710054China Printable Electronics Research Center Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou215123China Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun130022China

Layer-by-layer (LbL) strategy has been developed to form bulk heterojunction (BHJ) structure for processing efficient organic solar cells (OSCs). Herein, LbL slot-die coating with twin boiling point solvents (TBPS) strategy was developed to fabricate highly efficient OSCs, which matches with large-scale, high throughput roll-to-roll (R2R) industrialized mass process. The TBPS strategy could produce high-quality thin film without any additive, leading to the optimized vertical phase separation with interpenetrating nanostructures, as well as the enhanced charge transport and extraction. Thus, the power conversion efficiency up to 14.42% was achieved for [(2,6-(4,8-bis(5-(2-ethylhexyl-3-fluoro)thiophen-2-yl)-benzo [1,2-b:4,5-b′]dithiophene))-alt-(5,5-(1′,3′-di-2-thienyl-5′,7′-bis(2-ethylhexyl)benzo[1′,2′-c:4′,5′-c′]dithiophene-4,8-dione)]:2,2′-((2Z,2′Z)-((12,13-bis(2-ethylhexyl)-3,9-diundecyl-12,13-dihydro-[1,2,5]thiadiazolo[3,4-e]thieno[2″,3″:4″,5″]thieno[2′,3′:4,5]pyrrolo[3,2-g]thieno[2′,3′:4,5]thieno[3,2-b]indole-2,10-diyl)bis(methanylylidene)) bis(5,6-difluoro-3-oxo-2,3-dihydro-1H-indene-2,1-diylidene))dimalononitrile (PM6:Y6) OSCs fabricated via sequentially LbL slot-die coating using the TBPS strategy under ambient condition. The research provides a potential route for industrialized production of high-efficiency and large-area OSC devices.

关键词： organic solar cells slot-die coating twin boiling point solvent layer-by-layer(LbL) vertical phase separation

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蓝藻水华的短期监测预警与防控策略

环境科学与技术 2024年第S1期47卷 189-197页

作者：杨宏业陈迪袁云娟杨凯伦刘婉蓉黄舒欣屈铭志上海建科环境技术有限公司上海200032 武汉市水务局湖北武汉430010 湖北君邦环境技术有限责任公司湖北武汉430000

蓝藻水华问题持续影响着中国内陆湖泊,华中地区O湖近年6-10月均会受到蓝藻水华的侵扰,造成水体生物死亡,堆积与异味影响到了湖泊的休憩功能。该研究于2022年7-10月在O湖展开,通过对该湖蓝藻水华的持续低空监测与研究,分析其湖面分布情况... 详细信息

蓝藻水华问题持续影响着中国内陆湖泊,华中地区O湖近年6-10月均会受到蓝藻水华的侵扰,造成水体生物死亡,堆积与异味影响到了湖泊的休憩功能。该研究于2022年7-10月在O湖展开,通过对该湖蓝藻水华的持续低空监测与研究,分析其湖面分布情况,结合气象数据探究其漂移方向与规律,对其发展情况展开预测,并尝试通过物理阻隔等措施,减少其扩散范围。结果表明,2022年7月初O湖蓝藻主要分布在湖区东部区域,西部与南部区域蓝绿藻数量较低,未形成水华。通过气象数据分析结合自动站水质监测数据,提前预测蓝藻水华会由东部区域突破已设置的挡藻围隔进入西部湖区。因此,研究立即在O湖大桥布设档藻围隔,将水华控制在O湖东部区域,避免了其向其他区域漂移和扩散,使得O湖西部与南部区域未出现蓝藻水华。研究表明,水质自动站数据及无人机监测能够较准确地判断蓝藻的空间分布,结合气象数据能够预测蓝藻水华期的漂移,根据预测结果建设挡藻物理阻隔,能有效限制蓝藻水华的漂移,验证了蓝藻水华监控与防治方法的可行性,为蓝藻早期控制与治理提供了新的思路。

关键词：蓝藻水华无人机监测气象条件预测防控

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柑橘2个三倍体果实囊衣发育与化渣性评价

园艺学报 2024年第6期51卷 1179-1188页

作者：曹惠祥罗鑫刘婉荣管书萍王婷婷伍小萌郭文武解凯东华中农业大学园艺林学学院果蔬园艺作物种质创新与利用全国重点实验室武汉430070 襄阳市农业科学院湖北襄阳441100

探讨柑橘倍性杂交三倍体后代及其二倍体母本果实化渣性差异及原因,为柑橘三倍体果实化渣相关研究奠定数据基础。以本地早橘和红橘为对照,分别对以其为母本与四倍体倍性杂交获得的2个三倍体有性后代果实囊衣发育过程中的厚度变化和组分... 详细信息

探讨柑橘倍性杂交三倍体后代及其二倍体母本果实化渣性差异及原因,为柑橘三倍体果实化渣相关研究奠定数据基础。以本地早橘和红橘为对照,分别对以其为母本与四倍体倍性杂交获得的2个三倍体有性后代果实囊衣发育过程中的厚度变化和组分含量进行分析,探究囊衣结构及其成分对化渣性的影响。结果表明:2个三倍体后代果实囊衣厚度、细胞层数、硬度和剪切力在不同发育时期均显著大于其二倍体母本;与二倍体母本相比,2个三倍体后代囊衣细胞壁更厚,微纤丝密度更大,结构更紧密;囊衣的纤维素、半纤维素、水溶性果胶和原果胶含量均显著高于其二倍体母本,木质素含量则在部分发育时期显著低于二倍体母本。综上所述,果实囊衣厚,纤维素、半纤维素和果胶含量高可能是柑橘部分三倍体化渣性较差的主要原因,而这些指标可用于三倍体化渣性的评价和筛选。

关键词：柑橘三倍体囊衣化渣性纤维素含量

废弃矿山固废环境风险评价方法与分级监管建议

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有色金属（冶炼部分） 2024年第4期 55-62页

作者：韩伟陆子熠童立志王玉晶刘婉蓉赵瑞锋聂晶磊生态环境部固体废物与化学品管理技术中心北京100029 生态环境部土壤与农业农村生态环境监管技术中心北京100012 生态环境部华南环境科学研究所广州510655

中国是矿业大国,矿种丰富,造成历史遗留矿山众多。因矿种类型复杂、不同地区气候差异大、水文地质条件不同以及周边环境敏感度迥异,致使其环境风险不明,环境污染底数不清,目前仍缺乏针对历史遗留矿山环境风险的评价方法体系。本研究尝... 详细信息

中国是矿业大国,矿种丰富,造成历史遗留矿山众多。因矿种类型复杂、不同地区气候差异大、水文地质条件不同以及周边环境敏感度迥异,致使其环境风险不明,环境污染底数不清,目前仍缺乏针对历史遗留矿山环境风险的评价方法体系。本研究尝试建立了一种基于层次分析法的历史遗留矿山环境风险评估方法,选择具有主导作用、代表性的12项指标构建了基于评价目标层、评价准则层与评价因子层的历史遗留矿山固废环境风险评价三级指标体系,包括矿种类别、固废属性、自然条件、管理措施、保护目标与环境质量等,构建了判断矩阵并通过一致性检验,明确了各影响要素的权重。根据实际赋分情况,将历史遗留矿山环境风险等级分为一般、较大和严重三个等级。在此基础上提出了针对不同风险等级应采取的管理措施:自然衰减、风险管控与修复治理。最后将建立的评价方法应用于某地区的10个历史遗留矿山的环境风险评价,结果显示该地区一般、较大和严重风险的矿山数量分别为7、2和1,评价结果可为历史遗留矿山的差异性环境监管工作提供参考。

关键词：历史遗留矿山环境风险层次分析法分级环境监管

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滨岸带蓝藻水华无人机探测方法研究

环境科学与管理 2024年第2期49卷 111-115页

作者：杨宏业朱玉东刘婉蓉黄舒欣屈铭志上海建科环境技术有限公司上海200032 太湖流域水文水资源监测中心(太湖流域水环境监测中心) 江苏无锡214024

蓝藻进行探测时易出现影像质量差,探测精度低的情况,对此,研究滨岸带蓝藻水华无人机探测方法,文章以太湖作为研究对象,对太湖蓝藻堆积最严重的贡湖滨岸带展开探测方法研究,通过无人机水面飞行去光斑方法,获取滨岸带高质量照片,利用高表... 详细信息

蓝藻进行探测时易出现影像质量差,探测精度低的情况,对此,研究滨岸带蓝藻水华无人机探测方法,文章以太湖作为研究对象,对太湖蓝藻堆积最严重的贡湖滨岸带展开探测方法研究,通过无人机水面飞行去光斑方法,获取滨岸带高质量照片,利用高表真的蓝藻水面影像拼接技术将无人机照片拼接成高水面覆盖度和高表真性的数字正射影像,采用可见光蓝藻提取方法实现了蓝藻水华的识别与提取。实验结果表明,所提方法总体精度达到93.4%,Kappa系数达到0.8613,证明所提方法具有实用性。

关键词：蓝藻水华无人机探测高表真水面拼接

Wheat kinase TaSnRK2.4 forms a functional module with phosphatase TaPP2C01 and transcription factor TaABF2 to regulate drought response

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The Crop Journal 2024年第2期12卷 384-400页

作者： Yanyang Zhang Xiaoyang Hou Tianjiao Li Ziyi Wang Jiaqi Zhang Chunlin Zhang Xianchang liu Xinxin Shi wanrong Duan Kai Xiao State Key Laboratory of North China Crop Improvement and Regulation Baoding 071001HebeiChina College of Agronomy Hebei Agricultural UniversityBaoding 071001HebeiChina

SNF1-related protein kinase 2(SnRK2)family members are essential components of the plant abscisic acid(ABA)signaling pathway initiated by osmotic stress and triggering a drought stress response.This study characterized the molecular properties of TaSnRK2.4 and its function in mediating adaptation to drought in Triticum aestivum.Transcripts of TaSnRK2.4 were upregulated upon drought and ABA signaling and associated with drought-and ABA-responsive cis-elements ABRE and DRE,and MYB and MYC binding sites in the promoter as indicated by reporter GUS protein staining and activity driven by truncations of the promoter.Yeast two-hybrid,BiFC,and Co-IP assays indicated that TaSnRK2.4 protein interacts with TaPP2C01 and an ABF transcription factor(TF)TaABF2.The results suggested that TaSnRK2.4 forms a functional TaPP2C01-TaSnRK2.4-TaABF2 module with its upstream and downstream partners.Transgene analysis revealed that TaSnRK2.4 and TaABF2 positively regulate drought tolerance whereas TaPP2C01 acts negatively by modulating stomatal movement,osmotic adjustment,reactive oxygen species(ROS)homeostasis,and root morphology.Expression analysis,yeast one-hybrid,and transcriptional activation assays indicated that several osmotic stress-responsive genes,including TaSLAC1-4,TaP5CS3,TaSOD5,TaCAT1,and TaPIN4,are regulated by TaABF2.Transgene analysis verified their functions in positively regulating stomatal movement(TaSLAC1-4),proline accumulation(TaP5CS3),SOD activity(TaSOD5),CAT activity(TaCAT1),and root morphology(TaPIN4).There were high correlations between plant biomass and yield with module transcripts in a wheat variety panel cultivated under drought conditions in the field.Our findings provide insights into understanding plant drought response underlying the SnRK2 signaling pathway in common wheat.

关键词： Triticum aestivum SnRK2.4 kinase Gene expression Protein interaction Transgene analysis Transcriptional activation