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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第3期14卷 247-252页

作者： Sitthichai IAMSAARD Thawatchai PRABSATTROO wannisa sukhorum Supaporn MUCHIMAPURA Panee SRISAARD Nongnut UABUNDIT Wipawee THUKHAMMEE Jintanaporn WATTANATHORN Department of Anatomy Faculty of MedicineKhon Kaen University Integrative Complementary Alternative Medicine Research and Development Group Faculty of MedicineKhon Kaen University Department of Physiology Faculty of MedicineKhon Kaen University

Objective:To investigate the effect of Anethum graveolens(AG) extracts on the mounting frequency,histology of testis and epididymis,and sperm physiology.Methods:Male rats induced by cold immobilization before treating with vehicle or AG extracts [50,150,and 450 mg/kg body weight(BW)] via gastric tube for consecutive 1,7,and 14 d were examined for mounting frequency,testicular phosphorylation level by immunoblotting,sperm concentration,sperm acrosome reaction,and histological structures of testis and epididymis,respectively.Results:AG(50 mg/kg BW) significantly increased the mounting frequency on Days 1 and 7 compared to the control group.Additionally,rat testis treated with 50 mg/kg BW AG showed high levels of phosphorylated proteins as compared with the control group.In histological analyses,AG extract did not affect the sperm concentration,acrosome reaction,and histological structures of testis and epididymis.Conclusions:AG extract enhances the aphrodisiac activity and is not harmful to sperm and male reproductive organs.

关键词：外科学临床泌尿科学男子性机性障碍

Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds

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Journal of Integrative Medicine 2020年第3期18卷 265-273页

作者： Sitthichai Iamsaard Supatcharee Arun Jaturon Burawat Supataechasit Yannasithinon Saranya Tongpan Sudtida Bunsueb Natthapol Lapyuneyong Pannawat Choowong-in Nareelak Tangsrisakda Chadaporn Chaimontri wannisa sukhorum Department of Anatomy Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Research Institute for Human High Performance and Health Promotion Khon Kaen UniversityKhon Kaen 40002Thailand Faculty of Nursing Ratchathani UniversityUbon Ratchathani 34000Thailand School of Medicine Mae Fah Luang UniversityChiang Rai 57100Thailand

Objective: In traditional medicine, the seeds of Thai Mucuna pruriens(T-MP) are used to treat male dysuria and are believed to enhance fertility. However, information pertaining to the toxicity of T-MP and its interaction with other properties is limited. This study was thus conducted to evaluate the antioxidant capacity and subacute toxicity of T-MP in the reproductive system.Methods: Total phenolic content and antioxidant capacity of T-MP seed extract were determined using total phenolic content, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays.Male and female adult rats were treated orally with T-MP at a dosage of 150 or 300 mg/kg body weight for 14 consecutive days. Sex hormones and functional parameters in the liver and kidney were evaluated.Histopathology of all tissue was conducted using Masson’s trichrome staining. Sperm parameters, including concentration, morphology, acrosome reaction status and DNA damage, were also examined.Expression of tyrosine phosphorylated protein(Tyr Pho), androgen receptor and A-kinase-anchoring protein 4(AKAP4) were investigated using the Western blot technique.Results: T-MP seed extract contained phenolic compounds and exhibited high antioxidant capacity with no toxicity at the tested doses. It did not affect liver or kidney function parameters in the male rats, but increased estradiol, aspartate aminotransferase and alanine aminotransferase levels in the females.Additionally, it decreased serum progesterone and alkaline phosphatase levels in female rats. Serum and intratesticular testosterone levels were significantly lower in male rats that received a high dosage of T-MP. Histopathological changes were not observed in any tissue treated with T-MP. T-MP also significantly increased sperm concentration(but did not affect sperm parameters), and enhanced testicular Tyr Pho protein and androgen receptor and expression of AKAP4 in sperms.Conclusion: T-MP seed extract exhibited antioxidant capacity and was not harmful to reproductive tissues. It also had

关键词： Mamui Mucuna pruriens Reproductive system Subacute toxicity Rats

束缚应激对雄性大鼠睾丸磷酸化蛋白表达的影响（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第1期17卷 21-29页

作者： Supatcharee ARUN Jaturon BURAWAT wannisa sukhorum Apichakan SAMPANNANG Nongnut UABUNDIT Sitthichai IAMSAARD Department of Anatomy Faculty of MedicineKhon Kaen University Center for Research and Development of Herbal Health Products Faculty of Pharmaceutical SciencesKhon Kaen University

目的:寻找雄性大鼠应激模型中潜在的睾丸蛋白标记物,探索评估男性生殖能力新的参数。创新点:压力可以影响男性生育能力,但其具体机制尚未明了。睾丸组织中酪氨酸磷酸化蛋白在精子形成过程中扮演着重要的角色。本研究首次利用动物模型说... 详细信息

目的:寻找雄性大鼠应激模型中潜在的睾丸蛋白标记物,探索评估男性生殖能力新的参数。创新点:压力可以影响男性生育能力,但其具体机制尚未明了。睾丸组织中酪氨酸磷酸化蛋白在精子形成过程中扮演着重要的角色。本研究首次利用动物模型说明了压力对睾丸组织中酪氨酸磷酸化蛋白表达的影响,进而探索评估男性生殖能力新的参数。方法:雄性SD大鼠被随机分为对照组和束缚应激的实验组。实验组大鼠每天禁足12小时,持续7天。观察比较两组类固醇激素、精子密度、生殖系统形态学和组织学的差别,同时利用免疫印迹方法比较两组睾丸组织裂解产物中急性类固醇调节蛋白和酪氨酸的表达差别(早期在支持细胞中表达,后期在精子细胞中表达)。结果:实验组大鼠的睾酮水平和精子密度较对照组均显著降低,而精子畸形率和皮质酮显著升高。组织学比较发现实验组大鼠精子团数量减少并且出现输精管萎缩。两组大鼠睾丸组织中酪氨酸磷酸化蛋白的表达具有显著差异,实验组95 k Da的磷酸化蛋白表达量显著降低,但两组急性类固醇调节蛋白的表达没有显著差异。结论:本研究证明束缚应激可使大鼠睾丸组织磷酸化蛋白表达降低,并相应伴随着生殖能力参数的降低。这也许可以从一个方面解释压力对男性不育的影响。

关键词：束缚应激大鼠类固醇激素合成急性调节蛋白睾丸磷酸化蛋白

Phyllanthus emblica叶提取物对慢性应激大鼠睾丸损伤有改善作用（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第12期19卷 948-959页

作者： Supatcharee ARUN Jaturon BURAWAT Supataechasit YANNASITHINON wannisa sukhorum Akgpol LIMPONGSA Sitthichai IAMSAARD Department of Anatomy Faculty of Medicine Khon Kaen University Center for Research and Development of Herbal Health Product Faculty of Pharmaceutical Sciences School of Medicine Mae Fah Luang University

目的:探讨Phyllanthus emblica L.(PE)叶提取物对慢性应激大鼠睾丸损伤和相关蛋白标志物变化的影响。方法:将成年雄性大鼠分成四组:对照组、慢性应激(CS)组和两组PE提取物治疗组(50或100mg/kg体重(BW))。对各组的精子质量、睾丸和尾附... 详细信息

目的:探讨Phyllanthus emblica L.(PE)叶提取物对慢性应激大鼠睾丸损伤和相关蛋白标志物变化的影响。方法:将成年雄性大鼠分成四组:对照组、慢性应激(CS)组和两组PE提取物治疗组(50或100mg/kg体重(BW))。对各组的精子质量、睾丸和尾附睾的组织学特征、以及血清皮质酮、睾酮和丙二醛(MDA)的水平进行分析比较。并使用免疫印迹分析研究睾丸类固醇合成快速调节蛋白(StAR)和酪氨酸磷酸化蛋白的表达。结论:结果显示,50mg/kgBW的PE能显着增加精子浓度和睾酮水平,同时降低CS大鼠中皮质酮水平、MDA水平、以及精子头异常和顶体反应精子的比例。此外,两种剂量的PE都能缓解CS大鼠的睾丸组织病理学特征。同时我们还发现,50 mg/kg BW的PE可以显著提高StAR蛋白的表达,并改变睾丸中部分酪氨酸磷酸化蛋白的强度。总之,50 mg/kgBW的PE叶提取物可以预防CS大鼠的睾丸损伤。

关键词： Phyllanthusemblica L.叶提取物慢性应激睾丸损伤酪氨酸磷酸化

蝶豆花提取物的抗氧化活性及其对酮康唑诱导大鼠睾丸损伤的保护作用(英文)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第6期15卷 548-555页

作者： Sitthichai IAMSAARD Jaturon BURAWAT Pipatpong KANLA Supatcharee ARUN wannisa sukhorum Bungorn SRIPANIDKULCHAI Nongnut UABUNDIT Jintanaporn WATTATHORN Wiphawi HIPKAEO Duriya FONGMOON Hisatake KONDO Department of Anatomy and Integrative Complementary Alternative Medicine Research and Development Group Faculty of Medicine Khon Kaen University Center for Research and Development of Herbal Health Products Faculty of Pharmaceutical SciencesKhon Kaen University Department of Medical Services Lampang Cancer Hospital Ministry of Public health

研究目的:研究蝶豆花提取物的抗氧化活性及其对酮康唑(KET)诱导雄性大鼠睾丸损伤的保护作用。创新要点:对蝶豆花提取物保护酮康唑引起的雄性动物睾丸损伤进行研究,为减轻抗真菌药物酮康唑的生殖毒性提供理论依据和解决途径。研究方法:采... 详细信息

研究目的:研究蝶豆花提取物的抗氧化活性及其对酮康唑(KET)诱导雄性大鼠睾丸损伤的保护作用。创新要点:对蝶豆花提取物保护酮康唑引起的雄性动物睾丸损伤进行研究,为减轻抗真菌药物酮康唑的生殖毒性提供理论依据和解决途径。研究方法:采用2,2-二苯基-1-苦肼基自由基(DPPH)分析法和亚铁还原能力实验法(FRAP)来测定蝶豆花提取物的抗氧化活性。在为期28天的动物试验中,前21天为预防期,第21至28天为KET诱导期。整个试验期中,对雄性大鼠进行蝶豆花提取物(0、50、100 mg/kg BW)灌胃饲养;在诱导期,同时腹腔注射KET(100 mg/kg BW)。试验结束后,测定睾丸、附睾和输精管以及精囊的重量、血清睾酮水平、精子浓度、组织结构、睾丸的直径和睾丸酪氨酸磷酸化水平。重要结论:蝶豆花提取物具有清除DPPH自由基能力和较高的还原能力,其在100 mg/kg BW下对雄性大鼠生殖系统无毒。50、100 mg/kg BW蝶豆花提取物能改善KET引起的生殖器官重量下降、血清睾酮水平和精子浓度,降低KET引起的睾丸损伤。与其他组相比,100 mg/kg BW蝶豆花提取物能显著提高睾丸中50-kDa的酪氨酸磷酸化蛋白的表达。由此可见,具有抗氧化活性的蝶豆花提取物不会损伤雄性生殖系统,而且具有保护KET诱导大鼠睾丸损伤的作用。

关键词：蝶豆花提取物抗氧化活性酮康唑(KET) 睾丸损伤酪氨酸磷酸化

泰国余甘子水提物中总酚含量及抗氧化活性(英文)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第4期15卷 405-408页

作者： Sitthichai IAMSAARD Supatcharee ARUN Jaturon BURAWAT wannisa sukhorum Jintanaporn WATTANATHORN Somsak NUALKAEW Bungorn SRIPANIDKULCHAI Department of Anatomy Faculty of MedicineKhon Kaen University Center for Research and Development of Herbal Health Products Faculty of Pharmaceutical SciencesKhon Kaen University Integrative Complementary Alternative Medicine Research and Development Group Faculty of MedicineKhon Kaen University Department of Pharmaceutical Sciences Faculty of PharmacyMahasarakham University

通过对余甘子不同部位(叶、枝和树皮)水提物的总酚含量的测定及体外抗氧化活性的研究,为其体内及临床试验研究奠定了基础。余甘子叶、枝和树皮的水提物皆具有抗氧化活性,其中树皮中的总酚含量最高,抗氧化活性最强。

关键词：余甘子总酚含量抗氧化能力传统药用植物