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A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)

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Molecular Plant 2019年第4期12卷 582-596页

作者： Jun Liu wanjun hao Jing Liu Shihang Fan Wei Zhao Linbing Deng Xinfa Wang Zhiyong Hu Wei Hua Hanzhong Wang Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural Affairs Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Wuhan 430062 China Key Laboratory of Tea Biology and Resources Utilization Ministry of Agriculture and Rural Affairs Tea Research Institute of the Chinese Academy of Agricultural Sciences Hangzhou 310008 China

Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of great agricultural, nutritional, and economic importance. However, the genetic basis of CEs on SOC (CE-SOC) remains enigmatic. In this study, we use an optimized approach to sequence the cytoplasmic (plastid and mitochondrial) genomes of allotetraploid oilseed rape (Brassica napus) cultivars, 51218 and 56366, that bear contrasting CE-SOC. By combining comparative genomics and genome-wide transcriptome analysis, we identify mitochondria-encoded orf188 as a potential CE-SOC determinant gene. Functional analyses in the model system Arabidopsis thaliana and rapeseed demonstrated that orf188 governs CE-SOC and could significantly increase SOC, strikingly, through promoting the yield of ATP. Consistent with this finding, transcriptional profiling with microarray and RNA sequencing revealed that orf188 affects transcriptional reprogramming of mitochondrial energy metabolism to facilitate ATP production. Intriguingly, orf188 is a previously uncharacterized chimeric gene, and the presence of this genetic novelty endows rapeseed with positive CE-SOC. Our results shed light on the molecular basis of CEs on a key quantitative trait in polyploid crops and enrich the theory of maternal control of oil content, providing new scientific guidance for breeding high-oil rapeseed germplasms.

关键词： cytoplasmic effects seed oil content energy metabolism crop breeding Brassica napus

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纳米颗粒电场辅助冷喷涂数值研究

金属热处理 2015年第4期40卷 161-164页

作者：郑建新郝婉君河南理工大学机械与动力工程学院河南焦作454000

在喷管出口和基板之间外加电场来加速带电颗粒,并对带电纳米铜颗粒进行电场辅助冷喷涂气固两相流数值研究,分析在气流和电场力的综合作用下颗粒粒径、电荷量、基板电压等参数对颗粒速度的影响。结果表明,电场辅助冷喷涂不适合微米级颗粒... 详细信息

在喷管出口和基板之间外加电场来加速带电颗粒,并对带电纳米铜颗粒进行电场辅助冷喷涂气固两相流数值研究,分析在气流和电场力的综合作用下颗粒粒径、电荷量、基板电压等参数对颗粒速度的影响。结果表明,电场辅助冷喷涂不适合微米级颗粒,但非常适合纳米颗粒喷涂,且粒径越小,颗粒撞击速度越大,电场力加速效果越显著;颗粒电荷量、基板电压越大,颗粒撞击速度越高。

关键词：冷喷涂电场辅助冷喷涂静电场纳米颗粒数值研究

频率选择表面对PET水泥基材料吸波性能的影响

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建筑材料学报 2018年第1期21卷 117-123页

作者：张月芳郝万军刘顺华海南大学材料与化工学院海南海口570228 大连理工大学材料科学与工程学院辽宁大连116085

为解决电磁环境污染问题,以废弃聚对苯二甲酸乙二酯(PET)塑料瓶碎片、聚丙烯纤维作为水泥基体的透波剂和增韧增强介质,在水泥基材料表面复合封闭式、开放式、组合式3组频率选择表面(FSS),制备了成本低、质量轻、厚度薄、强度高、加工工... 详细信息

为解决电磁环境污染问题,以废弃聚对苯二甲酸乙二酯(PET)塑料瓶碎片、聚丙烯纤维作为水泥基体的透波剂和增韧增强介质,在水泥基材料表面复合封闭式、开放式、组合式3组频率选择表面(FSS),制备了成本低、质量轻、厚度薄、强度高、加工工艺简单的FSS复合水泥基吸波平板试样.结果表明:FSS复合水泥基吸波平板试样在2~18GHz频段的吸波性能有了较大的改善,环数为1环的组合式复合试样在2.5GHz处的最小反射率为-19.9dB,-10dB以下的吸收带宽占了整个频段的47.5%;S频段(2~4GHz)内FSS单元尺寸和个数对复合试样吸波性能的影响规律与4~18GHz频段表现出不同的特性.

关键词：水泥基吸波材料聚对苯二甲酸乙二酯(PET) 频率选择表面阻抗匹配 S频段

基于自适应广义滑模观测器的风力发电系统故障重构

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电力系统保护与控制 2021年第3期49卷 123-130页

作者：孙志辉郝万君曹松青王昊郝诗源周嘉玉苏州科技大学电子与信息工程学院江苏苏州215009 丹麦科技大学电气工程系哥本哈根灵比999017

针对风力发电系统的状态估计和执行器故障重构问题,提出一种自适应广义滑模观测器。通过构建一个增广系统,使得执行器故障成为系统状态的一部分。设计广义滑模观测器对增广系统进行状态估计从而获得所需的故障信息。针对实际风力发电系... 详细信息

针对风力发电系统的状态估计和执行器故障重构问题,提出一种自适应广义滑模观测器。通过构建一个增广系统,使得执行器故障成为系统状态的一部分。设计广义滑模观测器对增广系统进行状态估计从而获得所需的故障信息。针对实际风力发电系统中故障上界未知的问题,设计一种自适应算法来估计故障信息的上界。以5 MW的风电机组为研究对象,在Matlab/Simulink平台下进行仿真,结果证明了所提方法能够准确地实现系统状态以及故障的估计。

关键词：风力发电系统状态估计故障重构自适应算法广义滑模观测器

Enhancing SAEAs with unevaluated solutions:a case study of relation model for expensive optimization

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Science China(Information Sciences) 2024年第2期67卷 41-58页

作者： hao hao Xiaoqun ZHANG Aimin ZHOU Institute of Natural Sciences Shanghai Jiao Tong University Shanghai Frontiers Science Center of Molecule Intelligent Syntheses School of Computer Science and Technology East China Normal University

Surrogate-assisted evolutionary algorithms(SAEAs) hold significant importance in resolving expensive optimization problems. Extensive efforts have been devoted to improving the efficiency of SAEAs through the development of proficient model-assisted solution selection methods, which, however, first requires generating high-quality solutions. The fundamental paradigm of evaluating a limited number of solutions in each generation within SAEAs reduces the variance of adjacent populations, thereby affecting the quality of offspring solutions. This is a frequently encountered issue yet receives little attention. To address the issue, this paper presents a framework using unevaluated solutions to enhance the efficiency of *** surrogate model is employed to identify high-quality solutions for the direct generation of new solutions without evaluation. To ensure dependable selection, we have introduced two tailored relation models to select the optimal solution and the unevaluated population. A comprehensive experimental analysis is performed on two test suites, which showcases the superiority of the relation model over regression and classification models in the solution selection phase. Furthermore, the surrogate-selected unevaluated solutions with high potential have been shown to enhance the efficiency of the algorithm significantly.

关键词： expensive optimization unevaluated solutions relation model surrogate-assisted evolutionary algorithm

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电场辅助冷喷涂超微颗粒加速特性研究

热加工工艺 2014年第16期43卷 141-144页

作者：郑建新郝婉君河南理工大学机械与动力工程学院河南焦作454150

基于Fluent气固两相流数值模拟,研究电场辅助冷喷涂超微颗粒的加速特性,分析基板电压与喷涂距离对超微颗粒撞击速度的影响。结果表明:电场辅助冷喷涂中超微颗粒在电场力作用下能突破激波阻力,实现有效沉积;增大基板电压可提高颗粒撞击速... 详细信息

基于Fluent气固两相流数值模拟,研究电场辅助冷喷涂超微颗粒的加速特性,分析基板电压与喷涂距离对超微颗粒撞击速度的影响。结果表明:电场辅助冷喷涂中超微颗粒在电场力作用下能突破激波阻力,实现有效沉积;增大基板电压可提高颗粒撞击速度;大粒径颗粒的加速取决于惯性力,而超微颗粒的加速取决于电场力;同传统冷喷涂一样,电场辅助冷喷涂也存在最佳喷涂距离,且最佳喷涂距离与颗粒粒径无关。

关键词：冷喷涂电场辅助冷喷涂超微颗粒加速特性撞击速度

A Global Optimality Principle for Fully Coupled Mean-field Control Systems

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Acta Mathematicae Applicatae Sinica 2024年第2期40卷 379-413页

作者： Tao hao School of Statistics and Mathematics Shandong University of Finance and EconomicsJinan 250014China

This paper concerns a global optimality principle for fully coupled mean-field control *** the first-order and the second-order variational equations are fully coupled mean-field linear FBSDEs. A new linear relation is introduced, with which we successfully decouple the fully coupled first-order variational equations. We give a new second-order expansion of Y^(ε) that can work well in mean-field framework. Based on this result, the stochastic maximum principle is proved. The comparison with the stochastic maximum principle for controlled mean-field stochastic differential equations is supplied.

关键词： optimal control global maximum principle fully coupled general mean-field FBSDE adjoint equation recursive utility

Identification and Function of a Novel Gene,ORF188,Associated with Cytoplasmic Effects on Oil Content in Brassica napus

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Identification and Function of a Novel Gene,ORF188,Associate...

Machine learning-assisted smart epitaxy ofⅢ-Ⅴsemiconductors

2014年中国作物学会学术年会

作者： wanjun hao Wei Hua Shihang Fan Xinfa Wang Zhiyong Hu Jing Liu Hongli Yang Hanzhong Wang Key Laboratory for Biological Sciences of Oil Crops Ministry of AgricultureOil Crops Research InstituteChinese Academy of Agriculture Sciences College of Life Sciences Hubei University

Seed oil content(SOG) is an important agronomic trait in rapeseed(Brassica napus L.).Previous research showed cytoplasmic effect playing an important role on SOC in ***,the underlying molecular mechanisms remain largely *** this study,we identified and characterized a novel gene,ORF188,associated with cytoplasmic effect on SOC in *** on previous study,we obtained two rapeseed inbred lines 51218 and 56366,between which cytoplasmic effect had significant difference and reached seven per cent on *** understand the genetic bases,we sequenced plastid and mitochondrial genomes of the two rapeseed *** compared their plastid genomes and found many small genetic *** plastid genome,there were many small genetic variations between their mitochondrial *** surprisingly,it seemed rearrangements had happened between the two mitochondrial *** a result,there are fourteen different ORFs which are specific to one of the two mitochondrial ***,we conjectured that these fourteen specific ORFs are the reasons for different cytoplasmic effect on SOC between 51218 and *** further validate the conjecture,we compared the transcriptomics of reciprocal cross F which were made between 51218 and *** expected,there were only five cytoplasmic genes with differential ***,all these five genes were specific mitochondrial ***,this result validated our conjecture and reduced the candidate gene number to *** another way,a set of 48 rapeseed inbred lines possessing different types of cytoplasm were used for association *** measure cytoplasmic effect of the 48 rapeseed lines,reciprocal cross were made between 56366 and each one of *** cytoplasmic effects were identified as the difference of SOC between reciprocal cross ***,fourteen specific mitochondrial ORFs were designed as molecular marker and used for *** significant associations for cytoplasmic effect were d

关键词： Brassica napus cytoplasmic effect seed oil content ORF188 ATP

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Science China Materials 2024年第9期67卷 3041-3042页

作者： Yue hao Key Laboratory of Wide Band Gap Semiconductor Technology School of MicroelectronicsXidian UniversityXi’an710071China

Semiconductor epitaxial growth is the front-end process for manufacturing microelectronic and optoelectronic *** beam epitaxy(MBE),capable of precisely controlling atomic layer deposition,has emerged as a crucial technique in chip atomic-level manufacturing[1].However,in semiconductor epitaxy,the heterogeneous nature of materials is complicated,and the growth condition fluctuations poses significant challenges in defect suppression[2],substantially impacting chip performance and yield.

关键词： optoelectronic smart semiconductors