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The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq

Virologica Sinica 2016年第2期31卷 131-141页

作者： Yingying Shi Huilin Tu Xiong Chen Yingying Zhang liujun Chen Zhongchun liu Jiqun Sheng Song Han Jun Yin Biwen Peng Xiaohua He wanhong liu Pathogenic Organism and Infectious Diseases Research Institute School of Basic Medical SciencesWuhan University Wuhan 430071 China Hubei Province Key Laboratory of Allergy and Immunology Wuhan 430071 China Hubei Provincial Key Laboratory of Developmentally Originated Disease School of Basic Medical Sciences Wuhan University Wuhan 430071 China Institute of Neuropsychiatry Renmin Hospital Wuhan University Wuhan 430060 China College of Life Science and Technology Hubei Engineering University Xiaogan 432000 China

Coxsackievirus A16(CVA16) is one of major pathogens of hand, foot and mouth disease(HFMD) in children. Long non-coding RNAs(Inc RNAs) have been implicated in various biological processes,but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of Inc RNAs of normal and CVA16 infected rhabdomyosarcoma(RD)cells using RNA-Seq to investigate the functional relevance of Inc RNAs. We showed that a total of 760 Inc RNAs were upregulated and 1210 Inc RNAs were downregulated. Out of these dysregulated Inc RNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were s RNA host Inc RNAs and 0.05% were enhancer. Six dysregulated Inc RNAs were validated by quantitative PCR assays and the secondary structures of these Inc RNAs were projected. Moreover, we conducted a bioinformatics analysis of an Inc RNAs(ENST00000602478) to elucidate the diversity of modification and functions of Inc RNAs. In summary, the current study compared the dysregulated Inc RNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and Inc RNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD.

关键词：非编码RNA 柯萨奇病毒感染细胞 D细胞表达谱 RNA二级结构鉴定测序

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Preparation and evaluation of a new releasable PEGylated tumor necrosis factor-α(TNF-α) conjugate for therapeutic application

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Science China(Life Sciences) 2013年第1期56卷 51-58页

作者： DAI ChuanYun FU Ya CHEN ShaoCheng LI Biao YAO Bo liu wanhong ZHU LiQing CHEN Nan CHEN Ji ZHANG Qiang Department of Chemistry and Chemical Engineering Chongqing University of Science and Technology Department of Biological and Chemical Engineering Chongqing University of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking University

To design a releasable PEGylated TNF-α(rPEG-TNF-α ), a cathepsin B-sensitive dipeptide (Val-Cit moiety) was inserted into conventional PEG-modified TNF- (PEG-TNF- ), facilitating its clinical use for anti-tumor therapy. Comparative pharmaco- kinetic and pharmacodynamic studies showed that the half-lives of both PEGylated forms of TNF-α were ~60-fold greater than that of unmodified TNF-α . In addition, the in vitro bioactivity of rPEG-TNF-α was greater than that of PEG-TNF-α with the same degree of PEG modification. Release of TNF-α from rPEG-TNF-α in vitro was dependent on the presence of cathepsin B and was inhibited by a cathepsin B inhibitor. Despite the potent cytotoxicity of unmodified TNF-α against normal cells, its PEGylated forms at higher TNF-α concentrations showed low cytotoxic activity against these cells. In contrast, both forms of PEGylated TNF-α showed potent cytotoxic activity against the B16 and L929 cell lines, with rPEG-TNF-α being 5- and 9- fold more potent, respectively, than PEG-TNF-α . Moreover, rPEG-TNF-α was a more potent in vivo antitumor agent than PEG-TNF-α .

关键词：肿瘤坏死因子聚乙二醇化抗肿瘤治疗修饰组织蛋白酶B TNF-α 评价制备

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云南咖啡可持续种植的困境与路径选择

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黑龙江科学 2023年第17期14卷 143-145,148页

作者：刘婉虹中粮油脂控股有限公司北京10020

咖啡作为一种经济价值高的农作物,是种植区域内农户收入的主要来源,云南省的咖啡产量占全国的98%,随着全球咖啡消量的提升,扩大咖啡种植有利于增加农户收入。当前,云南省咖啡种植面临咖农获利较少,种植积极性较低和咖啡品牌效应较差,市... 详细信息

咖啡作为一种经济价值高的农作物,是种植区域内农户收入的主要来源,云南省的咖啡产量占全国的98%,随着全球咖啡消量的提升,扩大咖啡种植有利于增加农户收入。当前,云南省咖啡种植面临咖农获利较少,种植积极性较低和咖啡品牌效应较差,市场活力不足的问题。从保护环境视角出发,为咖啡的可持续种植提供基础;扩大种植面积,回应市场需求的快速增长;引导咖农建立自治组织和构建咖啡巢状市场是推动云南咖啡可持续种植的重要路径选择。

关键词：可持续种植咖啡云南巢状市场

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SYBR Green I-based product-enhanced reverse transcriptase assay for quantification of retroviral PFV and detection of the divalent cation preference of PFV RT

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Virologica Sinica 2014年第5期29卷 314-317页

作者： Xiaofang Yi Yan Sun Qingmei liu Zhi Li wanhong liu Xiaohua He Linjuan Zhang Yuxin Wang College of Life Sciences Shaanxi Normal University School of Medicine Wuhan University

Dear Editor,Prototype foamy virus(PFV)belongs to the genus Spumavirus in the Spumaretrovirinae subfamily of *** PFV and HIV have much in common,research into PFV has lagged far behind that into HIV,as PFV appeared to be non-pathogenic both in accidentally infected humans and in experimentally infected *** recent decades,however,more attention has been focused on PFV because it seems to

关键词： preference transcriptase subfamily pathogenic quantification divalent belongs behind infected template

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Lnc-RP5 Regulates the mi R-129-5p/Notch1/PFV Internal Promoter Axis to Promote the Expression of the Prototype Foamy Virus Transactivator Tas

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Virologica Sinica 2020年第1期35卷 73-82页

作者： Shanshan Xu liujun Chen Yinglian Tang Peipei Yuan Jun Yan Yingcheng Zheng Li Huang Zhi Li Yan Sun Song Han Jun Yin Qin Pan Biwen Peng Xiaohua He wanhong liu Department of Allergy Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Immunology and Hubei Province Key Laboratory of Allergy and Immunology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease Wuhan 430071China College of Life Sciences Shanxi Normal UniversityXi’an 710062China Centre for Medical Research Wuhan UniversityWuhan 430071China

Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical *** noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral ***,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected ***,little is known about the function of lnc-RP5 during PFV *** this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by ***,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of *** results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP *** work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.

关键词： Prototype foamy virus(PFV) lnc-RP5 Transactivator(Tas) Internal promoter miR-129-5p

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基于Notch信号通路探讨银屑平丸含药血清对TNF-α诱导的人表皮角质形成细胞相关蛋白及炎症因子的影响

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中药新药与临床药理 2023年第9期34卷 1171-1178页

作者：沈乐乐李淼彭子怡刘婉虹舒琪席建元湖南中医药大学湖南长沙410208 湖南中医药大学第一附属医院湖南长沙410000

目的基于调节Notch信号通路探讨银屑平丸含药血清对肿瘤坏死因子α(TNF-α)诱导的人表皮角质形成细胞相关蛋白及炎症因子的影响。方法根据中药血清药理学方法制备银屑平丸含药血清、阿维A含药血清,将血清分为5%、10%、20%3个浓度对人表... 详细信息

目的基于调节Notch信号通路探讨银屑平丸含药血清对肿瘤坏死因子α(TNF-α)诱导的人表皮角质形成细胞相关蛋白及炎症因子的影响。方法根据中药血清药理学方法制备银屑平丸含药血清、阿维A含药血清,将血清分为5%、10%、20%3个浓度对人表皮角质形成细胞进行干预,采用CCK-8试剂筛选含药血清最佳作用浓度;建立TNF-α诱导的银屑病细胞损伤模型,设置空白组、模型组、银屑平丸含药血清组、阿维A组、模型+γ-分泌酶抑制剂(DAPT)组、银屑平丸含药血清+DAPT组。采用ELISA法检测各组细胞上清液中TNF-α、白细胞介素6(IL-6)、白细胞介素8(IL-8)水平,采用Real-timePCR法检测Notch信号通路Notch1,Jagged-1表达,免疫荧光法检测各组细胞内皮蛋白(Involucrin)表达水平。结果TNF-α最佳作用浓度为10 ng·mL^(-1),银屑平丸含药血清最佳作用浓度为20%。与空白组比较,模型组可使各组细胞上清TNF-α、IL-6、IL-8水平升高(均P<0.0001),Notch1、Jagged-1表达上调(均P<0.0001),并使细胞Involucrin荧光表达增强(P<0.0001);与模型组比较,银屑平丸含药血清组可使细胞上清TNF-α、IL-6、IL-8水平降低(P<0.05,P<0.01),使Notch信号通路Notch1、Jagged-1表达下调(均P<0.0001),并使细胞Involucrin荧光表达减弱(P<0.05);与银屑平丸含药血清组比较,银屑平丸含药血清+DAPT组细胞上清TNF-α、IL-6、IL-8水平降低(P<0.0001,P<0.05,P<0.01),Notch1、Jagged-1表达下调(均P<0.0001),细胞Involucrin荧光表达减弱(P<0.05)。结论银屑平丸含药血清可抑制TNF-α诱导的人表皮角质形成细胞增殖,降低炎症因子表达水平及人表皮角质形成细胞相关蛋白表达,其机制可能与调节Notch信号通路有关。

关键词：银屑病银屑平丸 Notch信号通路人表皮角质形成细胞细胞增殖炎症因子

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The coxsackievirus A16 infection suppresses MAVS-induced apoptosis and type Ⅰ interferon activation

The coxsackievirus A16 infection suppresses MAVS-induced apo...

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第十一届全国免疫学学术大会

作者： Shi Yingying Tu Huilin Qiu Wenhong Huang Lixia Gong Yeli He Xiaohua liu wanhong Department of Immunology School of MedicineJianghan University Pathogenic Organism and Infectious Diseases Research Institute School of Basic Medical SciencesWuhan University

Background: Coxsackievirus A16(CA16) is one of the major causative agents of hand, foot and mouth disease(HFMD), which is characterized by herpetic lesions on the hands, feet and oral mucosa in children less than 5 years old. The mitochondrial antiviral signaling protein(MAVS) plays important roles in host defense against viral infection by inducing type I interferon. However, the relationship between CA16 infection and MAVS still remain ***: The aim of this study was to investigate the relationship between CA16 infection and ***: Quantitative real time PCR(qRT-PCR) and western blot were adopted to study the effect of CA16 infection on MAVS expression. Both overexpression and silencing of MAVS expression were employed to detect the effect of MAVS on CA16 replication. In addition, the plasmids over-expressing each viral nonstructural proteins were constructed and transfected into RD cells to investigate the influence of CA16 proteins on the functions of ***: CA16 infection inhibited MAVS expression. MAVS in turn suppressed CA16 replication by both apoptosis induction depending on JNK and ERK signals and type I interferon activation. We further demonstrated that viral protein 2C, 3D downregulated MAVS-induced apoptosis. Meanwhile, 2A and 3C greatly blocked the MAVS mediated activation of IFN-β. Furthermore, MAVS can be cleaved by 3C which is a vital ***: These data provide direct and compelling evidence that CA16 has evolved new strategies by which CA16 evades the host immune system and regulates apoptosis by targeting MAVS, which suggest new targets for anti-HFMD strategies.

关键词： Coxsackievirus A16(CA16) viral protein apoptosis type I interferon

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重症患者气道操作相关肠内营养喂养中断的研究进展

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中华护理杂志 2024年第12期59卷 1525-1530页

作者：刘欢杜爱平张耀丹尹万红田永明四川大学华西医院重症医学科成都市610041

气道操作导致的肠内营养喂养中断是重症患者肠内营养中断的重要因素,也是导致肠内营养喂养不达标的主要原因之一。反复或长时间的喂养中断会加剧患者肠内营养喂养不足,阻碍其康复并增加并发症的发生。由于缺乏明确的指南和规范,重症患... 详细信息

气道操作导致的肠内营养喂养中断是重症患者肠内营养中断的重要因素,也是导致肠内营养喂养不达标的主要原因之一。反复或长时间的喂养中断会加剧患者肠内营养喂养不足,阻碍其康复并增加并发症的发生。由于缺乏明确的指南和规范,重症患者在气道操作相关肠内营养喂养中断的临床实践上存在较大差异。该研究综述了重症患者气道操作相关肠内营养喂养中断的临床意义、临床实践,并提出未来的研究方向,旨在为提升重症患者气道操作相关肠内营养喂养中断实践的规范性、安全性和有效性提供参考。

关键词：气道操作肠内营养喂养中断气管插管气管切开拔管护理综述

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全球等温扩增技术发展态势研究

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武汉大学学报（医学版） 2023年第6期44卷 647-653页

作者：张丽月王姗姗何祯硕闫君卢晅刘万红武汉大学泰康医学院(基础医学院)过敏及免疫相关疾病湖北省重点实验室湖北武汉430071

目的:研究等温扩增技术发展态势,为我国开展相关研究提供数据参考。方法:通过文献计量方法、科学知识图谱等方法对等温扩增技术及新型冠状病毒(SARS-CoV-2)等温扩增检测相关的研究论文进行数据分析和知识挖掘,研究技术发展脉络、研究热... 详细信息

目的:研究等温扩增技术发展态势,为我国开展相关研究提供数据参考。方法:通过文献计量方法、科学知识图谱等方法对等温扩增技术及新型冠状病毒(SARS-CoV-2)等温扩增检测相关的研究论文进行数据分析和知识挖掘,研究技术发展脉络、研究热点和发展趋势。结果:中国在等温扩增技术研究上具有较强的研究基础,SARS-CoV-2等温扩增检测技术研究处于全球领先水平。中国科学院等国内机构成为全球等温扩增技术的重要研究力量。研究较多的等温扩增技术主要为环介导等温扩增(LAMP)、滚环扩增(RCA)、重组酶聚合酶扩增(RPA)和依赖核酸序列的扩增(NASBA)。主要的研究对象为SARS-CoV-2、HIV、HPV、Salmonella和Malaria等病毒和细菌及癌症标记物。重点关注的技术工具为CRISPR/Cas基因编辑、生物传感器、微流控芯片等。结论:2000年以前,等温扩增技术主要集中于NASBA和链替代扩增(SDA)技术;2000年以后,LAMP、RCA等新技术出现,LAMP迅速成为主流技术。新型冠状病毒肺炎疫情发生以来,等温扩增技术广泛应用于SARS-CoV-2的快速性和便捷性检测。

关键词：等温扩增技术知识图谱研究热点文献计量学新型冠状病毒

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Advance in immunology of human enterovirus 71

Advance in immunology of human enterovirus 71

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2012全国临床微生物与感染免疫学术研讨会

作者： liu wanhong,Zhou Bingfei,He Xiaohua School of Basic Medical Sciences,Wuhan University,Wuhan430071,China

Human enterovirus 71(EV71) is a common causative agent of hand,foot and mouth disease in young *** can infect central nervous system(CNS) and cause neurological diseases such as aseptic meningitis, encephalo-myelitis,brain stem encephalitis,acute flaccid paralysis even ***,its neurological pathogenesis remains elusive to *** it had been reported that it may be associated with the immunopathological ***,we reviewed the present studies of EV71 inimmunology in respects of receptor,antibody,cytokine, immune cells and vaccine,which may promote evidence in the following further *** may point the way that what we can do in this field and provide new approaches toward antivirus drug and vaccine.

关键词： EV71 Receptor A ntibody Cytokine Immune cells Vaccine

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