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Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha

Journal of Integrative Agriculture 2024年第6期23卷 1929-1939页

作者： Yingzhen Wang Ying Wu Xinlei Wang wangmei ren Qinyao Chen Sijia Zhang Feng Zhang Yunzhi Lin Junyang Yue Yongsheng Liu School of Horticulture Anhui Agricultural UniversityHefei 230036China School of Forestry Science and Technology Lishui Vocational and Technical CollegeLishui 323000China College of Life Science Sichuan UniversityChengdu 610064China

Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular *** this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly *** study provides valuable insights into the genetic basis of variation in *** fruit traits,which may benefit molecular marker-assisted breeding efforts.

关键词： Actinidia eriantha GWAS SNP QTLs fruit quality

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Telomere‑to‑telomere and haplotype‑resolved genome of the kiwifruit Actinidia eriantha

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Molecular Horticulture 2023年第1期3卷 361-371页

作者： Yingzhen Wang Minhui Dong Ying Wu Feng Zhang wangmei ren Yunzhi Lin Qinyao Chen Sijia Zhang Junyang Yue Yongsheng Liu School of Horticulture Anhui Agricultural UniversityHefei 230036China School of Forestry Science and Technology Lishui Vocational and Technical CollegeLishui 323000China Ministry of Education Key Laboratory for Bio‑Resource and Eco‑Environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China

Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease *** has been used in a wide range of breeding programs and functional genomics *** published genome assemblies of *** are quite fragmented and not highly *** multiple sequencing strategies,we get the haplotype-resolved and gap-free genomes of an elite breeding line“Midao 31”(MD),termed MDHAPA and *** new assemblies anchored to 29 pseudochromosome pairs with a length of 619.3 Mb and 611.7 Mb,as well as resolved 27 and 28 gap-close chromosomes in a telomere-to-telomere(T2T)*** on the haplotype-resolved genome,we found that most alleles experienced purifying selection and coordinately *** to the high continuity of assemblies,we defined the centromeric regions of ***,and identified the major repeating monomer,which is designated as *** resource lays a solid foundation for further functional genomics study and horticultural traits improvement in kiwifruit.

关键词： Kiwifruit Actinidia eriantha Genome Telomere-to-telomere Haplotype Centromeric

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无创呼吸机干预在新生儿急救护理中应用的效果观察

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现代科学仪器 2021年第3期38卷 164-166页

作者：任雁周望梅刘丹南方医科大学南方医院急诊科 510515

目的:探讨新生儿急救护理中应用无创呼吸机干预的效果。方法:本次研究对象共64例,是我院收治的呼吸困难新生儿,选取时间段为2018年3月-2019年3月。按照电脑随机分配原则,其中32例患者接受头罩吸氧治疗方法,视为对照组;另外32例患者接受... 详细信息

目的:探讨新生儿急救护理中应用无创呼吸机干预的效果。方法:本次研究对象共64例,是我院收治的呼吸困难新生儿,选取时间段为2018年3月-2019年3月。按照电脑随机分配原则,其中32例患者接受头罩吸氧治疗方法,视为对照组;另外32例患者接受无创呼吸机干预,视为观察组。比较观察两组患儿的治疗效果以及治疗前后血气指标变化。结果:经过治疗干预后,观察组患儿的治疗有效率显著优于对照组(96.88%VS78.13%),数据比较差异较大,且观察组患儿并发症发生率低6.25%(P0.05),经过治疗干预后,两组相关的血气指标均得到改善,但观察组更为明显(P<0.05)。结论:应用无创呼吸机干预方法,有助于提高新生儿急救治疗效果,且患儿的血气指标也能得到显著改善,应用价值较高。

关键词：新生儿急救护理无创呼吸机应用效果

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