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Al对M2高速钢铸态组织的影响

特殊钢 2009年第3期30卷 52-54页

作者：周雪峰方峰蒋建清李德辉朱旺龙东南大学江苏省先进金属材料高技术研究重点实验室南京211189 天工集团丹阳215400

试验M2高速钢(%:0.85C、5.92W、4.74Mo、3.86Cr、1.81V)由25 kg真空感应炉熔炼,并用砂型铸造成锭。研究了0～1.3%Al对钢铸态组织的影响。结果表明,加入0.6%Al,可细化共晶莱氏体网,改善碳化物分布;加入过量Al(1.3%),初生晶粒内部出现大... 详细信息

试验M2高速钢(%:0.85C、5.92W、4.74Mo、3.86Cr、1.81V)由25 kg真空感应炉熔炼,并用砂型铸造成锭。研究了0～1.3%Al对钢铸态组织的影响。结果表明,加入0.6%Al,可细化共晶莱氏体网,改善碳化物分布;加入过量Al(1.3%),初生晶粒内部出现大量由δ铁素体通过共析转变产生的针状碳化物。Al促进M_2C共晶碳化物形态由弯曲棒状变为平直片层状,并提高其合金元素含量。与片层状M_2C相比,棒状M_2C在高温易分解成短棒状或球状碳化物,有利改善碳化物形态。

关键词： Al 高速钢M2 铸态组织 M2C共晶碳化物

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树鼩分类研究进展

绿色科技 2021年第16期23卷 128-130,135页

作者：冯佳红王政昆朱万龙云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南师范大学生命科学学院云南昆明650500

基于树鼩(Tupaia belangeri)与人类有更高亲缘性的论断和其自身特点,使其被迅速开发为模式生物和各种疾病模型。但树鼩分类的研究仍无明确定论,这为其广泛应用埋下了隐患。从古生物学、分子生物学、形态学和表观遗传学等方面讨论了树鼩... 详细信息

基于树鼩(Tupaia belangeri)与人类有更高亲缘性的论断和其自身特点,使其被迅速开发为模式生物和各种疾病模型。但树鼩分类的研究仍无明确定论,这为其广泛应用埋下了隐患。从古生物学、分子生物学、形态学和表观遗传学等方面讨论了树鼩分类的最新研究进展,并对它们所面临的问题进行了展望:在古生物学方面,新的树鼩化石证据表明,树鼩出现时间较之前的研究早了一倍;在分子生物学方面,利用单分子测序技术所获得的更准确的全基因组序列,为树鼩基因组建树打下了更坚实基础;在形态学方面,通过与小鼠和恒河猴等物种的皮肤解剖结构比较,认为其更亲缘于灵长目。此外,随着表观遗传学理论的逐步完善,为树鼩分类的研究又打开了一扇新的大门。

关键词：树鼩分类模式生物古生物学

Regulatory mechanism and functional analysis of S100A9 in acute promyelocytic leukemia cells

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Frontiers of Medicine 2017年第1期11卷 87-96页

作者： Yonglan zhu Fang Zhang Shanzhen Zhang wanglong Deng Huiyong Fan Haiwei Wang Ji Zhang State Key Laboratory of Medical Genomics Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Medical Institute of Health Sciences Chinese Academy of Sciences Shanghai 200025 China

S100A9, a calcium-binding protein, participates in the inflammatory process and development of various tumors, thus attracting much attention in the field of cancer biology. This study aimed to investigate the regulatory mechanism of SIOOA9 and its function involvement in APL. We used real-time quantitative PCR to determine whether PML/RARα affects the expression of S100A9 in NB4 and PR9 cells upon ATRA treatment. ChiP-based PCR and dual-luciferase reporter assay system were used to detect how PML/RARα and PU.1 regulate S100A9 promoter activity. CCK-8 assay and flow cytometry were employed to observe the viability and apoptosis of NB4 cells when S100A9 was overexpressed. Results showed that SIOOA9 was an ATRA-responsive gene, and PML/RARa was necessary for the ATRA-induced expression of SlOOA9 in APL cells. In addition, PU.1 could bind to the promoter of S100A9, especially when treated with ATRA in NB4 cells, and promote its activity. More importantly, overexpression of S100A9 induced the apoptosis of NB4 cells and inhibited cell growth. Collectively, our data indicated that PML/RARα and PU.1 were necessary for the ATRA-induced expression of S100A9 in APL cells. Furthermore, S100A9 promoted apoptosis in APL cells and affected cell growth.

关键词： S100A9 PU. 1 PML/RARα ATRA APL

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基于粪便DNA的中缅树鼩性别鉴定

绿色科技 2017年第22期19卷 131-133页

作者：谌立新梅丽张海姬侯东敏朱万龙王政昆云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南昆明650500 云南师范大学生命科学学院云南昆明650500

指出了SRY基因是存在于哺乳动物雄性Y染色体上的性别决定基因,对该基因的检测可以快速准确地判定性别。利用分子粪便学方法从野外获取中缅树鼩粪便中提取到的基因组DNA,运用双重PCR同时扩增SRY基因和微卫星DNA后,经过电泳分析最终鉴定出... 详细信息

指出了SRY基因是存在于哺乳动物雄性Y染色体上的性别决定基因,对该基因的检测可以快速准确地判定性别。利用分子粪便学方法从野外获取中缅树鼩粪便中提取到的基因组DNA,运用双重PCR同时扩增SRY基因和微卫星DNA后,经过电泳分析最终鉴定出了79只树鼩的性别。

关键词：中缅树鼩粪便DNA SRY基因性别鉴定

下肢康复机器人在脑卒中偏瘫患者运动功能障碍中的应用研究

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中国疗养医学 2021年第4期30卷 350-352页

作者：孟祥凤商晓英冯天甜王涤徐浩繁朱旺龙黑龙江中医药大学 150040 黑龙江省医院 150036

近年来,脑卒中患病人数逐年上升,偏瘫是脑卒中患者经常遗留的运动功能障碍,而康复训练可有效降低脑卒中偏瘫患者的致残率,但常规康复治疗难以实现负重、迈步、平衡的有机结合,因此寻求积极有效的康复手段成为临床研究热点。大量研究表明... 详细信息

近年来,脑卒中患病人数逐年上升,偏瘫是脑卒中患者经常遗留的运动功能障碍,而康复训练可有效降低脑卒中偏瘫患者的致残率,但常规康复治疗难以实现负重、迈步、平衡的有机结合,因此寻求积极有效的康复手段成为临床研究热点。大量研究表明,延长康复治疗干预时间或增加训练次数对运动功能障碍的改善有较为积极的作用。而下肢康复机器人可让患者模拟正常运动模式不断重复的系统化练习,达到降低治疗师工作强度和增加患者主动参与度的目的,优于单一的常规康复治疗,在临床呈现更佳的治疗效果。

关键词：下肢机器人脑卒中偏瘫运动功能综述

Characterization of three African swine fever viruses from diferent clinical settings revealed a potential attenuation mechanism

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Animal Diseases 2024年第4期4卷 263-274页

作者： Jiajia Zhang Yuening Wang Kaili Zhang Dafu Deng Kai Peng wanglong Zheng Nanhua Chen Penggang Liu Shaobin Shang Ruichao Li Jianzhong zhu College Veterinary Medicine Yangzhou UniversityYangzhouChina Joint International Research Laboratory of Agriculture and Agri‑Product Safety Yangzhou UniversityYangzhouChina Comparative Medicine Research Institute Yangzhou UniversityYangzhouChina Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009China

African swine fever(ASF)is an acute and fatal hemorrhagic disease in domestic pigs and wild boars caused by African swine fever virus(ASFV)that currently threatens the pig industry *** the 2018 ASF outbreak in China,ASFV has evolved and caused diverse clinical manifestations,such as chronic and asymptomatic ***,it is important to understand the molecular mechanisms underlying ASFV attenuation in the ***,we isolated three ASFVs from one diseased and two asymptomatic pigs by using primary porcine alveolar macrophages(PAMs)from both domestic pigs and Bama *** three ASFVs exhibited similar phenotypes in cell culture,includ‑ing cytopathic efects(CPEs),hemadsorptions(HADs),viral protein expressions and growth *** sequenc‑ing revealed that all three ASFVs were genotype II *** comparisons suggested that the disruption of the viral genes MGF360 and MGF110,rather than EP402R and EP153R,is likely involved in the potential attenuation of ASFV via the upregulation of innate immune responses.

关键词： African swine fever virus Virus isolation Genome sequence Genotype II Attenuation

Gut microbiota,inflammation,and molecular signatures of host response to infection

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Journal of Genetics and Genomics 2021年第9期48卷 792-802页

作者： wanglong Gou Yuanqing Fu Liang Yue Geng-Dong Chen Xue Cai Menglei Shuai Fengzhe Xu Xiao Yi Hao Chen Yi zhu Mian-Li Xiao Zengliang Jiang Zelei Miao Congmei Xiao Bo Shen Xiaomai Wu Haihong Zhao Wenhua Ling Jun Wang Yu-Ming Chen Tiannan Guo Ju-Sheng Zheng College of Life Sciences Zhejiang UniversityHangzhou 310058China Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province School of Life SciencesWestlake UniversityHangzhou 310024China Center for Infectious Disease Research Westlake Laboratory of Life Sciences and BiomedicineHangzhou 310024China Institute of Basic Medical Sciences Westlake Institute for Advanced StudyHangzhou 310024China Key Laboratory of Structural Biology of Zhejiang Province School of Life SciencesWestlake UniversityHangzhou 310024China Guangdong Provincial Key Laboratory of Food Nutrition and HealthDepartment of EpidemiologySchool of Public HealthSun Yat-sen UniversityGuangzhou 510275China Taizhou Hospital Wenzhou Medical UniversityLinhai 325035China CAS Key Laboratory for Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Gut microbial dysbiosis has been linked to many noncommunicable ***,little is known about specific gut microbiota composition and its correlated metabolites associated with molecular signatures underlying host response to ***,we describe the construction of a proteomic risk score based on 20 blood proteomic biomarkers,which have recently been identified as molecular signatures predicting the progression of the *** demonstrate that in our cohort of 990 healthy individuals without infection,this proteomic risk score is positively associated with proinflammatory cytokines mainly among older,but not younger,*** further discover that a core set of gut microbiota can accurately predict the above proteomic biomarkers among 301 individuals using a machine learning model and that these gut microbiota features are highly correlated with proinflammatory cytokines in another independent set of 366 *** metabolomics analysis suggests potential amino acid-related pathways linking gut microbiota to host metabolism and ***,our multi-omics analyses suggest that gut microbiota composition and function are closely related to inflammation and molecular signatures of host response to infection among healthy *** results may provide novel insights into the cross-talk between gut microbiota and host immune system.

关键词： Gut microbiota Proinflammatory cytokines Proteomic biomarkers COVID-19 Host infection response