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Acta Oceanologica Sinica 2009年第4期28卷 72-77页

作者： wang Xubo ZHANG Quanqi CHEN Yanjie QI Jie wang zhigang wang Xinglian Key Laboratory of Marine Genetics and Breeding of Ministry of Education College of Marine Life Sciences Ocean University of China Qingdao 266003 China

A cytogenetic analysis of Paralichthys olivaceus was carried out using the flow cytometry method for DNA content, silver staining for the nucleolus organizer region （AgNORs） identification and one-color fluorescence in situ hybridization （FISH） for chromosomal mapping of major ribosomal genes. Nuclear DNA content was estimated by flow cytometry method using Gallus domesticus erythrocytes as the internal reference standard. The C-value of this species was （0.737±0.024） pg, and the DNA contents of each chromosome were estimated to be 16.51 Mb to 39.50 Mb after paired according to the average relative length. The FISH probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals and AgNORs were both located on the secondary constrictions of chromosome 1. These results will provide a better understanding of the cytogenetic information of this species and would help for further research of the karyotype evolution in the order Pleuronectiformes.

关键词： DNA content rDNA AgNORs FISH Paralichthys olivaceus

Novel Functions of Interleukin-1 Beta in Spotted Halibut(Verasper variegatus) at Its Embryonic and Metamorphic Stages

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Journal of Ocean University of China 2018年第5期17卷 1197-1208页

作者： LI Zan LIU Xiumei CAO DANDan LIU Yuezhong wang Xubo LIU Jinxiang wang zhigang ZHANG Quanqi YU Haiyang School of Agriculture Ludong University Key Laboratory of Marine Genetics and Breeding of Ministry of Education College of Marine Life SciencesOcean University of China

Interleukin-lbeta （IL-1β） is a cytokine involved in the control of inflammatory responses. The functions of IL-1β have been extensively studied in many vertebrates. However, few studies focused on the functions of IL-1β in the spotted halibut （Verasper variegatus）, an endangered aquatic fish that easily suffers from bacterial and viral infections. We first cloned and sequenced the IL-1β gene from V. variegatus （VvIL-1β）. Its conservative interleukin-1 propeptide and interleukin-1/18 domains corresponding regions, characteristics of the IL-1 protein family and TA-rich motifs relating to inflammatory responses were similar to those of the IL-1β genes from other teleost and mammals. The phylogenetic analysis showed that VvIL-1β was homologous to the IL-1β gene of other vertebrates. Treatment with pathogen-associated molecular patterns （PAMPs） induced a significant and rapid up-regulation of VvIL-1β expression in the peripheral blood leukocytes （PBLs）, revealing that VvIL-1β is actively involved in the host ilmnune responses against bacterial and viral pathogens. The expression level of VvIL-1β was relatively high in the PBLs, gill, and intestine, indicating the immunological function of VvIL-1β . VvIL-1β also had interesting expression patterns at specific developmental stages, implying that the IL-1β of flatfishes may have some novel functions during embryonic development and metamorphosis. The 3D structure prediction also provided information about how VvIL-113 interacts with its receptor protein. These results suggested that VvIL-1β may have multiple functions in V. variegatus and provided us valuable information for understanding the novel functions of IL- 1β.

关键词： IL-1β Verasper variegatus expression pattern metamorphosis peripheral blood leukocyte 3D modeling pathogenassociated molecular pattern

Unitary cyclic ESPRIT-like direction finding

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Science China(Information Sciences) 2010年第10期53卷 2087-2096页

作者： LIU zhigang & wang JinKuan Department of Electronic Information, Northeastern University, Qinhuangdao 066004, China 1. Department of Electronic Information Northeastern University Qinhuangdao 066004 China

A unitary formulation of cyclic ESPRIT algorithm, with uniform linear array (ULA) configuration, is proposed by constructing the forward backward cyclic autocorrelation matrix by Hermitian mapping. This approach not only substantially reduces the computational complexity via real-valued decomposition, but also outperforms the forward backward spatial smoothing algorithm for uncorrelated or correlated sources. In addition, this method allows to select desired signals and to ignore interferences because of its signal-selective property. The performance analysis shows that for uncorrelated or correlated sources, the Hermitian mapping approach has a lower RMSE and a higher output SNR than the FBSS method. Finally, simulation results show the effectiveness of the proposed methods.

关键词： array signal processing cyclostationary signals direction of arrival cyclic ESPRIT unitary ESPRIT

Estimation of generalized soil structure index based on differential spectra of different orders by multivariate assessment

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International Soil and Water Conservation Research 2024年第2期12卷 313-321页

作者： Sha Yang zhigang wang Chenbo Yang Chao wang Ziyang wang Xiaobin Yan Xingxing Qiao Meichen Feng Lujie Xiao Fahad Shafiq Wude Yang College of Agriculture Shanxi Agriculture UniversityTaiguChina College of Smart Agriculture Shanxi Agriculture UniversityTaiguChina Department of Botany Government College University LahorePakistan

Better soil structure promotes extension of plant roots thereby improving plant growth and *** in soil structure can be determined by changes in the three phases of soil,which in turn affect soil function and fertility *** compare the quality of soil structure under different conditions,we used Generalized Soil Structure Index(GSSI)as an indicator to determine the relationship between the“input”of soil three phases and the“output”of soil *** achieve optimum monitoring of comprehensive indicators,we used Successive Projections Algorithm(SPA)for differential processing based on 0.0–2.0 fractional orders and 3.0–10.0 integer orders and select important wavelengths to process soil spectral *** addition,we also applied multivariate regression learning models including Gaussian Process Regression(GPR)and Artificial Neural Network(ANN),exploring potential capabilities of hyperspectral in predicting *** results showed that spectral reflection,mainly contributed by long-wave near-infrared radiation had an inverse relationship with GSSI *** wavelengths between 404-418 nm and 2193–2400 nm were important GSSI wavelengths in fractional differential spectroscopy data,while those ranging from 543 to 999 nm were important GSSI wavelengths in integer differential spectroscopy ***,non-linear models were more accurate than linear *** addition,wide neural networks were best suited for establishing fractional-order differentiation and second-order differentiation models,while fine Gaussian support vector machines were best suited for establishing first-order differentiation *** terms of preprocessing,a differential order of 0.9 was found as the best *** the results,we propose that when constructing optimal prediction models,it is necessary to consider indicators,differential orders,and model *** all,this study provided a new method for an in-depth analyses of generalized soil *** also fills

关键词： Three-phase soil Generalized soil structure index Hyperspectral Differential spectrum Regression learning model

Two-phase anaerobic co-digestion of dairy manure with swine manure

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International Journal of Agricultural and Biological Engineering 2016年第2期9卷 146-152页

作者： Guan Zhengjun Sun Xianli Bi Lanping Li Wenzhe Zhang Ying wang zhigang College of Engineering Northeast Agricultural UniversityHarbin 150030China Qingdao Tianren Environment Co. LtdQingdao 266101China Qiqihar University Qiqihar 161006HeilongjiangChina

In order to solve the problems associated with high fiber content,and the ensuing lower biogas volume yield in anaerobic digestion of dairy manure,a study of the co-digestion of separated liquids from dairy manure combined with swine manure using a two-phase anaerobic digestion process was *** influence of level of total solids(TS)and hydraulic retention time(HRT)of the mixed liquor on the specific methane production were *** TS levels 8%,10%and 12%were *** of the results show that a maximum specific methane yield of 132.99 L/kg volatile solids(VS),can be obtained with a TS of 9%,an inoculation rate of 30%,the duration of hydrolytic acidification phase of 5 d,and an HRT of the methanogenic phase of 10 *** findings could provide directions for improving the biogas production by performing the co-digestion of dairy manure with swine manure.

关键词： co-digestion two-phase anaerobic digestion dairy manure swine manure biogas

A Generalization of σ-Permutability

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Communications in Mathematics and Statistics 2022年第3期10卷 565-579页

作者： zhigang wang Jin Guo Inna N.Safonova Alexander N.Skiba School of Science Hainan UniversityHaikou 570228HainanPeople’s Republic of China Department of Applied Mathematics and Computer Science Belarusian State University220030 MinskBelarus Department of Mathematics and Technologies of Programming Francisk Skorina Gomel State University246019 GomelBelarus

Throughout this paper,all groups are finite and G always denotes a finite group;σis some partition of the set of all primes P.A group G is said to beσ-primary if G is aπ-group for someπ∈σ.Aπ-semiprojector of G[29]is a subgroup H of G such that HN/N is a maximalπ-subgroup of G/N for all normal subgroups N of ***П⊆σ.Then we say thatχ={X_(1),...,X_(t)}is aП-covering subgroup system for a subgroup H in G if all members of the setχareσ-primary subgroups of G and for eachπ∈Пwithπ∩π(H)≠φthere are an index i and aπ-semiprojector U of H such that U≤X_(i).We study the embedding properties of subgroups H of G under the hypothesis that G has aП-covering subgroup systemχsuch that H permutes with X^(x)for all X∈χand x∈*** well-known results are generalized.

关键词： Finite group σ-nilpotent group П-semiprojector П-covering subgroup system σ-subnormal subgroup

Tissue Expression and Stock Variation of Isozymes of Stone Flounder(Kareius bicoloratus)

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Journal of Ocean University of China 2007年第2期6卷 153-160页

作者： XU Jianpeng ZHANG Quanqi QI Jie wang zhigang Laboratory of Genetics and Breeding College of Marine Life Science Ocean University of China Qingdao 266003 P. R. China

Tissue expression and stock variation of isozymes of stone flounder （Kareius bicoloratus） were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The results indicated that all the isozymes examined were obviously tissue-specific. The expressions of SOD＊, GDH＊, G3PDH-2＊ and ADH-2＊ were detected only in liver, SDH-1＊, MDH-1＊ and ADH-1＊ only in muscle, and LDH-B＊ and LDH-C＊ only in eyes. In comparison, MDH-2＊, GPI-3＊ and SDH-2＊ were detected in all tissues examined. Other loci examined were detected in a variety of tissues. Muscle and liver were selected to detect the isozyme variation of the two geographic stocks of Qingdao and Weihai, Shandong Province, China. The percentages of polymorphic loci （P0.99） were 29.17% and 25.00%, the observed heterozygosities （H0） were 0.028 ±0.014 and 0.040 ± 0.019, and the expected heterozygosities （He） were 0.039±0.017 and 0.052±0.022 in Qingdao and Wethai stock, respectively. The coefficient of gene differentiation （Fst） and genetic distance （D） between the two stocks was 0.012 and 0.0011, respectively, indicating that the genetic differentiation is low between them. Compared with other species of Pleuronectiformes, both the percentage of polymorphic loci and the mean heterozygosity ofK. Bicoloratus were at a middle level.

关键词： stone flounder Kareius bicoloratus isozyme tissue expression stock variation

Identification of two Major Histocompatibility(MH) Class Ⅱ A Genes and Their Association to Vibrio anguillarum Infection in Half-smooth Tongue Sole(Cynoglossus semilaevis)

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Journal of Ocean University of China 2012年第1期11卷 32-44页

作者： LI Chunmei wang Xubo ZHANG Quanqi wang zhigang QI Jie YI Qilin LIU Zhipeng wang Yanan YU Haiyang College of Marine Life Sciences Key Laboratory of Marine Genetics and BreedingMinistry of EducationOcean University of ChinaQingdao 266003P.R.China

Major histocompatibility complex class II antigens are important in vertebrate immune *** the present study,the full cDNA sequence of class II A gene was synthesized by RACE-PCR from half-smooth tongue sole(Cynoglossus semilaevis),and its open reading frame(ORF) polymorphism was *** whole cDNA sequence was 992 bp in length,including the ORF with 717 ***-five alleles were identified and clustered into two distinct groups according to the specific nucleotides/amino acids in specific *** alleles belonged to Cyse-DAA while fourteen alleles belonged to *** Cyse-DAA alleles were observed in one individual,and three to five Cyse-DBA alleles were observed in each of the three detected individuals,which indicated that at least two loci existed in each ***,in order to study the function of the alleles in resistance to infection,200 individuals were intraperitoneally injected with Vibrio anguillarum and the first 20 dead individuals and 20 surviving ones were selected for genotype ***-six alleles were identified among the 40 ***-nine alleles belonged to Cyse-DAA and the other 27 alleles belonged to *** alleles were selected for studying their function in resistance to *** Cyse-DAA*0201,Cyse-DAA*1101,Cyse-DBA*0401,Cyse-DBA*1102,Cyse-DBA*1801 and Cyse-DBA*2201 were identi-fied only in surviving individuals,while alleles Cyse-DAA*0901,Cyse-DBA*1101 and Cyse-DBA*1401 occurred more frequently in dead *** study confirmed the existence and polymorphism of two class II A genes as well as the relationship between alleles of class II A genes and disease susceptibility/resistance in half-smooth tongue sole.

关键词： major histocompatibility polymorphism Cyse-DAA Cyse-DBA allele Vibrio anguillarum

基于层次化P2P协议的网格空间数据库系统模型

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武汉大学学报（信息科学版） 2008年第12期33卷 1233-1236,1258页

作者：孟令奎张文 Frank zhigang wang 武汉大学遥感信息工程学院武汉市430079 剑桥-克兰菲尔德高性能计算设施网格计算中心 AMAC/SOE克兰菲尔德大学英国Bedfordshire MK43OAL

针对传统空间数据库技术的不足,充分结合网格空间数据管理的新特点,提出了一种适合于网格环境的空间数据库系统模型(grid peer-spatial database management system,GPeer-SDBMS)。该模型运用P2P协议Tapestry构建了基于网格的层次化分... 详细信息

针对传统空间数据库技术的不足,充分结合网格空间数据管理的新特点,提出了一种适合于网格环境的空间数据库系统模型(grid peer-spatial database management system,GPeer-SDBMS)。该模型运用P2P协议Tapestry构建了基于网格的层次化分布式空间数据库,不仅利用数据库的模式差异将网格空间数据库划分为由相同模式节点组成的多个虚拟节点集合,实现了空间数据的分布式存储,而且还对查询算法进行了有效的改进,并通过实验验证了查询算法的高效性。

关键词：网格空间数据库 P2P Tapestry 分布式

Locally differentially private frequency distribution estimation with relative error optimization

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Frontiers of Computer Science 2024年第5期18卷 227-229页

作者： Ning wangI Yifei LIU zhigang wang Zhiqiang WEI Ruichun TANG Peng TANG Ge YU Cyberspace Institute of Advanced Technology Guangzhou UniversityGuangzhou 510006China School of Computer Science and Technology Ocean University of ChinaQingdao 266100China Key Laboratory of Cryptologic Technology and Information Security(Ministry of Education) Shandong UniversityQingdao 266071China School of Computer Science and Technology Northeastern UniversityShenyang 110819China

This paper tackles the problem of estimating the frequency distribution on the crowdsourced multidimensional categorical data under local differential privacy(LDP).Although the frequency estimation problem under LDP[1]has attracted a lot of attention in recent years,currently,to our knowledge,the existing works are all devoted to optimizing the absolute error,rather than relative *** the work for the former,the one targeting at the latter should take the true frequency distribution into consider and design true frequency distribution-sensitive data collection protocol so that the skewed distribution,which involves smaller frequency in high probability,is with less ***,it is challenging to fulfill such a requirement,since the true frequency distribution is private information and not available.

关键词： distribution estimation error