检索结果-南通市图书馆

Relationship between cellular radio-sensitivity and naked DNA damage in mammalian cells exposed to heavy ions

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2009年第6期20卷 349-353页

作者： LI Wenjian ZHOU Guangming wang zhuanzi LI Qiang DANG Bingrong wang Jufang Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China

The relationship between deoxyribonucleic acid (DNA) damage and the cell death induced by 12C ions irradiation was examined in four kinds of cells, Melanoma B16, cervical squamous carcinoma HeLa, Chinese hamster V79 and hepatoma SMMC-7721. Cell survival was determined by a colonogenic assay, and the sensitivity was described by D50 (the dose of radiation necessary to reduce the survival to 50%). For all cell lines, D50 ranged from 0.74 Gy to 3.85 Gy, among them B16 was the most radiosensitive to 12C ions, and V79 and HeLa cells had almost the same radio-sensitivity, SMMC-7721 was the last. The induction of deproteinized DNA double-strand breaks induced by 12C ions were measured by pulsed-field gel electrophoresis (PFGE), and the initial yield of the deproteinized DNA dsbs per unit dose was expressed as the DNA double break level (L). A linear dose-response curve was seen for initial DNA dsbs for all cell lines (slopes range from 0.40-0.98 (DSBs/100Mbp/Gy)). V79 was the steepest, B16 was the last. There was an inverse relationship between the initial DNA dsb and D50 if the B16 cell line was not considered, but there was no relativity even excludes the B16 cell line. The present results indicate that there is no relationship between cellular sensitivity and initial DNA dsb, even exclude the effects of chromatin structure.

关键词：哺乳动物细胞 DNA损伤放射敏感性重离子 DNA双链断裂脉冲场凝胶电泳无线蜂窝

Protective Effect of Beta-D-glucan on Cell Injury Induced by Carbon Ion Irradiation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2018年第1期 137-138页

作者： wang zhuanzi Liu Fang Li Wenjian Wei Wei Dang Bingrong 不详

Charged particle radiation is known to be more biologically effective than photon *** example of this is the damage of human blood vessels,and this effect is an important factor influencing human health and is relevant to space travel as well as to cancer ***,research on the protection endothelial cells from heavy ions radiation damage is of great *** showed that beta-D-glucan is one of the potential radioprotectors[1],but most of the studies were focused on the protection of beta-glucan on the immune and hematopoietic after X-/-rays *** the present study,we chose human umbilical endothelial cell line ***926 as a model system to investigate the protective effect of beta-D-glucan on the injury induced by high-LET carbon ions irradiation,and explore the possible mechanism of the action.

关键词： protective damage travel

In Vivo Protective Effect of beta-D-glucan on X-rays Radiation Injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2017年第1期 130-131页

作者： wang zhuanzi Liu Fang Li Wenjian Wei Wei Dang Bingrong 不详

Ionizing radiation is a powerful treatment modality against tumor cells,but it is not tumor specific and may affect neighboring normal cell as *** to normal tissue is an obstacle to radiotherapy of ***,strategies to protect cancer patients from the harmful side effects of radiation without compromising the anti-cancer effects are of considerable interest in *** against ionizing radiation is also important during accidental and unavoidable exposures to radiation,such as nuclear industries,radiation accidents,space travels,radiation diagnosis,etc。

关键词： diagnosis cancer travel

Damage and Transcriptional Response of Murine Bone Marrow Cells Exposed to Total-body Carbon Ion Irradiation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2018年第1期 136-137页

作者： wang zhuanzi Liu Fang Li Wenjian Wei Wei Dang Bingrong 不详

It becomes more importance to understand the toxic effects of heavy ion irradiation on normal tissue as more people spend more time in the space environment and as carbon ion radiotherapy become increasingly used in the treatment of *** tissue damage due to therapeutic or accidental radiation exposure is a pervasive *** marrow,the predominant site of hematopoiesis and the differentiation of blood cells,is extremely sensitive to ionizing *** with even as little as 0.1 Gy X-ray could cause a significant increase in micronucleated reticulocytes in *** to heavy ion irradiation can have even more detrimental effects on bone marrow *** induction of bone marrow damage cannot be predicted correctly as the biological and molecular mechanisms altered after irradiation is still largely *** and characterization of these molecular processes is not only imperative for the development of protective measures for cancer patients undergoing high LET radiotherapy,but also for astronauts in space.

关键词： protective damage predominant

4-37 DNA Damage in Bone Marrow Mononuclear Cells of Mice Detected by Two Dimensional Comet Assay

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2016年第1期 155-156页

作者： wang zhuanzi Liu Fang Li Wenjian Wei Wei Dang Bingrong 不详

Heavy ion irradiation attract a large interest for two applications:radiotherapy and space radiation protection in manned space *** to heavy ions radiation results in multiple effects through DNA damage ***-cell gel electrophoresis or comet assay is known for its ability to detect DNA damage at the single cell level and has been used for years to assess DNA *** can detect low levels of DNA strand breaks in a short time,just using a few sample *** double strand breaks(DSBs)are measured at the neutral comet assay condition;under the alkaline comet assay condition both DNA single strand breaks(SSBs)and part DSBs are *** two dimensional comet assay is a modification of the two original comet assay,can simultaneously detect DNA SSBs and DSBs in the same human spermatozoa.

关键词：

4-38 DNA Damage in Bone Marrow Mononuclear Cells of Mice Following Total Body Irradiation with Carbon Ions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2016年第1期 156-157页

作者： wang zhuanzi Liu Fang Li Wenjian Wei Wei Dang Bingrong 不详

Among the many special factors in the space environment,space radiation especially high energy heavy ion radiation is widely regarded as one of the crucial factors that hazard the health and safety of *** is well known that biological effects of radiation are dependent on the linear-energy-transfer(LET)of the emergent *** ions have high LET and could induce complex and clustered DNA damage,which is more difficult to be repaired than individual lesions and is even irreparable.

关键词：

Micronuclei Induction in Human Lymphocytes Induced by Carbon Ions Exposure along Penetrate Depth of Ions in Water

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

近代物理研究所和兰州重离子加速器实验室年报：英文版 2009年第1期 118-119页

作者： wang zhuanzi Li Wenjian Xing Xiaoting Qu Yin Jing Xigang School of Life Science and Technology Shanxi University Taiyuan （China）.

G2-Chromosome fragments after irradiation with Carbon ions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物物理学报 2009年第S1期25卷 480-481页

作者： wang zhuanzi, Li Wenjian, Wei Wei, Jing Xigang, Dang bingrong, Liu jinting Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Nanchang road 509

Measurements of chromosome aberrations in peripheral blood lymphocytes are currently the most sensitive and reliable indicator of radiation exposure that can be used for

关键词： Carbon ions G2-Chromosome fragments PCC technique

Relationship between SMC1 Protein Expression and S-phase Arrest of Two Cell Lines by Different LET Radiation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

近代物理研究所和兰州重离子加速器实验室年报：英文版 2009年第1期 137-138页

作者： Wei Wei Li Wenjian Jing Xigang Dang Bingrong wang Biqian Zhang Bintuan wang zhuanzi