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民办高校党建工作与思想政治工作深度融合探究

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南昌师范学院学报 2024年第1期45卷 54-58页

作者：王素芳广州科技职业技术大学马克思主义学院广东广州510550

高校党建工作与思想政治工作是同源的,在本质上具有一致性,两者在实践中具有契合性、交互性。当前民办高校在党建工作与思想政治工作深度融合方面的主要问题是没有完全形成立德树人的大党建大思政机制,突出难点是这种机制的构建在实践... 详细信息

高校党建工作与思想政治工作是同源的,在本质上具有一致性,两者在实践中具有契合性、交互性。当前民办高校在党建工作与思想政治工作深度融合方面的主要问题是没有完全形成立德树人的大党建大思政机制,突出难点是这种机制的构建在实践中遇到阻力,加上同向发力的育人载体不明确,使得党建工作与思想政治工作的深度融合发生矛盾。要改变这种状况,就要找准两者深度融合的结合点和着力点,构建民办高校党建工作与思想政治工作深度融合的决策引领机制、实践育人机制、实施机制和保障机制。

关键词：民办高校党建工作思想政治工作深度融合

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欧盟《新电池法规》深度解析

上海汽车 2024年第7期 45-48,53页

作者：吴姣王素芳上汽集团创新研究开发总院上海201804

欧盟《新电池法规》的发布,引起了我国新能源汽车企业和电池生产企业的高度关注,特别是关于其中的碳足迹和尽职调查要求,应该如何应对成了热门话题。文章分析了欧盟电池法规的背景和主要内容,与国内政策进行了系统性对比,提出了国内政... 详细信息

欧盟《新电池法规》的发布,引起了我国新能源汽车企业和电池生产企业的高度关注,特别是关于其中的碳足迹和尽职调查要求,应该如何应对成了热门话题。文章分析了欧盟电池法规的背景和主要内容,与国内政策进行了系统性对比,提出了国内政策和企业的应对建议。

关键词：动力电池碳足迹尽职调查新能源汽车

采用SDS研究泥蚶血红蛋白(Tg-Hb)Ⅱ具有过氧化物酶及抗菌活性的结构基础

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海洋科学 2024年第2期48卷 24-31页

作者：濮丽丽王诗怡林志华王素芳包永波上海海洋大学水产与生命学院上海201306 浙江万里学院生物与环境学院浙江省水产种质资源高效利用技术研究重点实验室浙江宁波315100 浙江万里学院宁海海洋生物种业研究院浙江宁海315604

泥蚶血红蛋白(Tg-Hb)Ⅱ除了具有携氧功能外,还具有过氧化物酶活性和抗菌活性。本文采用分光光度法、光谱法和分子对接等技术研究了十二烷基硫酸钠(SDS)对Tg-HbⅡ的结构、过氧化物酶活性及抗菌活性的影响,以探讨Tg-HbⅡ具有过氧化物酶活... 详细信息

泥蚶血红蛋白(Tg-Hb)Ⅱ除了具有携氧功能外,还具有过氧化物酶活性和抗菌活性。本文采用分光光度法、光谱法和分子对接等技术研究了十二烷基硫酸钠(SDS)对Tg-HbⅡ的结构、过氧化物酶活性及抗菌活性的影响,以探讨Tg-HbⅡ具有过氧化物酶活性及抗菌活性的结构基础。研究结果显示,SDS的疏水烷基长链可嵌入到Tg-HbⅡ血红素口袋内部,与近端His104形成氢键,断裂血红素铁与His104的配位键,使Tg-HbⅡ的Soret带吸收峰降低并发生位移;此外,SDS还可与血红素口袋中的氨基酸形成疏水相互作用,改变血红素口袋原有结构,使得部分疏水氨基酸暴露,导致外源荧光强度增强,最大发射波长红移。SDS可以抑制Tg-HbⅡ的过氧化物酶活性,当SDS浓度为2 mmol·L^(-1)时,Tg-HbⅡ的酶活性仅为原来的20%,在琼脂扩散实验中失去对枯草芽孢杆菌的抗菌活性。以上结果表明,SDS通过破坏血红素口袋的内部结构抑制Tg-HbⅡ的过氧化物酶活性,使其失去抗菌活性,血红素疏水口袋是Tg-HbⅡ具有过氧化物酶活性和抗菌活性的关键结构。本研究为进一步研究Tg-HbⅡ的抗菌机理奠定基础。

关键词：泥蚶血红蛋白十二烷基硫酸钠过氧化物酶活性抗菌活性血红素口袋

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Synergistic effects of phosphoric acid modified hydrochar and coal gangue-based zeolite on bioavailability and accumulation of cadmium and lead in contaminated soil

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Chinese Journal of Chemical Engineering 2022年第6期35卷 150-160页

作者： Qilong Ge Qi Tian sufang wang Fang Zhu College of Environmental Science and Engineering Taiyuan University of TechnologyTaiyuan 030024China Department of Architecture and Environmental Engineering Taiyuan CollegeTaiyuan 030032China College of Civil Engineering Taiyuan University of TechnologyTaiyuan 030024China

In this paper,a novel compound was developed by mixing H_(3)PO_(4)-modified cauliflower leaves hydrochar(CLH)and coal gangue-based Na-X zeolite(ZL).An alkaline soil contaminated with cadmium(Cd)and lead(Pb)was amended through the individual and synergistic application of CLH and ZL(1%CLH,2%CLH,1%ZL,2%ZL and 1%CLH+1%ZL),and Chinese cabbage was grown on *** application of CLH was superior to ZL on decreasing the pH of alkaline soil and increasing soil available phosphorus(Olsen-P)and soil organic matter(SOM).In contrast,their combined application significantly improved the soil cation exchange capacity(CEC).Besides,the 1%CLH+1%ZL was the most efficient treatment in decreasing diethylenetriamine pentaacetate(DTPA)-extractable Cd/Pb and concentrations of these two metals in cabbage root and *** synergistic application could better increase Cd and Pb immobilization and cabbage yield than their alone ***,the immobilization of Pb for all treatments was higher than that of *** synergistic immobilization mechanism of CLH and ZL reflected that the CLH precipitated and complexed with these two metals,which may block the pores of hydrochar or wrap on the surface of *** the continuous adsorption and complexation were ***,ZL could probably alleviate this *** finding provides helpful information about using CLH combined with ZL as a soil stabilizer to immobilize heavy metals in contaminated alkaline soil.

关键词： H_(3)PO_(4)-modified hydrochar Coal gangue-based zeolite Mixture Immobilization Heavy metal pollution Soil remediation

气相色谱-质谱法测定沉积物中的烷基酚和双酚A

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环境化学 2024年第3期43卷 1064-1068页

作者：汪素芳曹莹董淮晋冯学良高存富魏占亮中国环境科学研究院环境检测与实验中心北京100012

本文以内分泌干扰物烷基酚和双酚A为研究对象,使用加速溶剂萃取-硅胶柱净化-气相色谱质谱联用仪,建立了同时测定沉积物中8种痕量APs和BPA的检测方法.沉积物样品经冷冻干燥后,用丙酮-二氯甲烷(V/V=1/2)为萃取溶剂,在100℃下进行加速溶剂... 详细信息

本文以内分泌干扰物烷基酚和双酚A为研究对象,使用加速溶剂萃取-硅胶柱净化-气相色谱质谱联用仪,建立了同时测定沉积物中8种痕量APs和BPA的检测方法.沉积物样品经冷冻干燥后,用丙酮-二氯甲烷(V/V=1/2)为萃取溶剂,在100℃下进行加速溶剂萃取.萃取液浓缩后使用硅胶柱净化,经气相色谱分离、质谱检测,根据保留时间、质谱碎片及其丰度定性,内标法定量.结果显示,待测物在10—2000μg·L^(-1)范围内线性关系良好,相关系数大于0.9994,检出限在0.6—1.9μg·kg^(-1)之间.目标物在200、500μg·L^(-1)标准溶液中相对标准偏差(n=7)为1.9%—5.4%,加标样品回收率为85.7%—99.5%.用本方法检测天津某河流的沉积物样品,4-丁基苯酚、4-叔辛基苯酚、4-壬基酚、双酚A被检出,实际样品中APs和BPA的加标回收率为84.5%—97.4%.实验表明,使用该方法检测沉积物中的APs和BPA,操作简便,灵敏度高,准确性和重现性良好,可满足沉积物中痕量APs和BPA的检测需要.

关键词：气相色谱-质谱法烷基酚类化合物双酚A 沉积物

2017—2023年临沂市食源性疾病患者沙门菌感染情况及病原特征分析

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中国初级卫生保健 2024年第7期38卷 83-86页

作者：王济忠王素芳李涛蒋海英左亚凡临沂市疾病预防控制中心山东临沂276000 国家电网公司北京电力医院北京100073

目的:了解2017—2023年山东省临沂市食源性疾病患者中沙门菌的感染情况并进行分析,探讨可能的传播途径和影响因素,为相关部门制定防控措施提供参考依据。方法:收集2017—2023年临沂市食源性疾病监测数据,分析患者的年龄、性别、职业分... 详细信息

目的:了解2017—2023年山东省临沂市食源性疾病患者中沙门菌的感染情况并进行分析,探讨可能的传播途径和影响因素,为相关部门制定防控措施提供参考依据。方法:收集2017—2023年临沂市食源性疾病监测数据,分析患者的年龄、性别、职业分布、发病季节性变化及临床表现等情况,并对分离出的沙门菌进行血清分型及耐药性敏感试验。结果:2017—2023年临沂市共采集符合病例定义的标本3267份,检测分离134株沙门菌,检出率为4.10%,其中2023年检出率最高(7.10%);感染病例主要发生在夏秋季;进食场所主要发生在家庭;0~6岁年龄段人群沙门菌检出率最高(5.75%);职业为家务及待业的人群沙门菌检出率最高(6.03%),其次为儿童(5.71%);沙门菌血清型分布以鼠伤寒和肠炎沙门菌为主;耐3种及以上多重抗药菌株数为107株(79.85%)。结论:相关部门应持续做好对沙门菌疾病的监测预警工作,提高食品安全隐患的早期识别、预警和控制能力,从而减少食源性疾病的发生。

关键词：沙门菌药物敏感性实验血清型

Synthesis of stable 2-sulfoniophenolate

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Chinese Science Bulletin 2000年第16期45卷 1480-1484页

作者： HOU Zijie wang sufang PAN Xianhua Institute of Organic Chemistry and State Key Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000China

A series of novel stable 2-sulfoniophenolates (5a-5e, 7a, 7b) were synthesized for the first time by the reactions of 3,7-dinitrodibenzobromolium bisulfate (3) with some sulfoxides and the reactions of 3,7-dinitrodibenzocycloiodonium salts (6a, 6b) with dimethyl sulfoxide under basic condition. Their structures were established by elemental analysis, NMR, MS, IR, etc. The role of the amine (or other basic compounds, such as NaOH, Na2CO3, etc.) in the reactions was discussed. A possible reaction mechanism was proposed, by which a dissymmetric substituted benzyne was presumed to be the reaction intermediate.

关键词： 2-suIfoniophenolate synthesis structure reaction mechanism.

Masculinizer gene controls sexual differentiation in Hyphantria cunea

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Insect Science 2024年第2期31卷 405-416页

作者： Xiaowei Li Huihui Liu Honglun Bi Yaohui wang Jun Xu sufang Zhang Zhen Zhang Ze Zhang Yongping Huang Laboratory of Evolutionary and Functional Genomics School of Life SciencesChongqing UniversityChongqingChina CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Centerfor Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghaiChina Key Laboratory of Forest Protection Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of ForestryState Forestry AdministrationBeijingChina

The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx *** the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest *** the present study,we identified and functionally characterized the Masc gene,HcMasc,in *** analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell ***,expression data showed that unlike male-biased expression in ***,Hc-Masc gene expresses in main all developmental stages or tissues in both *** regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both *** results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in *** and provide a foundation for developing better strategies to control this pest.

关键词： CRISPR/Cas9 dosage compensation Hyphantria cunea Masc sexual differentiation

Initial stiffness and moment resistance of reinforced joint with end-plate connection

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Frontiers of Structural and Civil Engineering 2009年第4期3卷 345-351页

作者： sufang wang Yiyi CHEN Shanghai University of Electric Power Shanghai 201300China College of Civil Engineering Tongji UniversityShanghai 200092China

In beam-to-column joint with bolted end-plate connection,the structural details of column flange reinforced by backing plate and column web panel reinforced by supplementary plate are *** joint is divided into some basic components,and the initial stiffness of each component is ***,the initial stiffness of reinforced components is drawn by theoretical model and finite element *** initial stiffness of reinforced joint can be obtained by assembling the initial stiffness of each *** design moment resistance of column flange reinforced by backing plate is deduced based on yield line method,and the design moment resistances of other components are deduced based on present *** design moment resistance of the reinforced joint is then determined by the minimum of the design moment resistances of all *** comparison with the results of finite element calculation,it is verified that the method to calculate the initial stiffness of reinforced joint is accurate enough to be used to estimate the rigid behavior of the joint and to make parametric study.

关键词： steel structure end-plate connection backing plate supplementary web plate initial stiffness moment resistance