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miR-34a通过激活lncRNA NORHA转录诱导猪卵巢颗粒细胞早期凋亡

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中国农业科学 2024年第5期57卷 1000-1009页

作者：王思琪周春雪李玉琦杜星潘增祥李齐发南京农业大学动物科技学院

【目的】探索miR-34a诱导GCs凋亡的RNA激活机制，为揭示猪卵泡闭锁的分子机理、筛选调控母猪繁殖的小核酸调节物提供依据。【方法】利用生物信息学方法分析猪miR-34a特征及其与潜在靶基因lncRNA NORHA启动子的结合情况。利用核质分离技... 详细信息

【目的】探索miR-34a诱导GCs凋亡的RNA激活机制，为揭示猪卵泡闭锁的分子机理、筛选调控母猪繁殖的小核酸调节物提供依据。【方法】利用生物信息学方法分析猪miR-34a特征及其与潜在靶基因lncRNA NORHA启动子的结合情况。利用核质分离技术分析猪GCs中miR-34a的亚细胞定位。qPCR检测miR-34a对猪GCs中NORHA、凋亡标志基因BCL-2和BAX表达的影响。利用荧光素酶活性分析验证miR-34a对NORHA启动子转录活性的靶向调控关系。利用染色质免疫沉淀(ChIP)检测miR-34a对猪GCs中NORHA启动子miRNA反应元件(MRE)处AGO2和组蛋白修饰因子富集情况。利用western blot和流式细胞术分析miR-34a通过NORHA对下游因子FOXO1的表达和细胞凋亡的调控作用。【结果】猪miR-34a为基因间miRNA，其序列在哺乳动物中具有高度保守性。亚细胞定位分析显示，miR-34a在猪GCs中细胞核与细胞质均有分布。生物信息学分析显示miR-34a的MRE位于NORHA启动子-194 nt至-173 nt处，且两者结合自由能为-23.9 kcal·mol-1。qPCR结果表明miR-34a显著上调猪GCs中NORHA的表达。荧光素酶活性分析显示，miR-34a极显著上调NORHA启动子报告载体活性，而对MRE突变型启动子活性没有显著影响。ChIP试验显示，miR-34a可增加NORHA启动子MRE处RNA诱导转录激活(RITA)复合物核心成员AGO2和转录激活型组蛋白H3K4me3的富集，而减少转录抑制型组蛋白H3K9me3的富集。共转试验显示，敲减NORHA可显著或极显著逆转由miR-34a过表达引起的FOXO1蛋白水平和早期凋亡率的上调，以及BCL-2/BAX比值的下调。【结论】细胞核miR-34a是猪GCs中的内源性小激活RNA(saRNA)，通过靶向激活促凋亡lncRNA NORHA的转录，诱导猪GCs早期凋亡(细胞膜完整)，可能参与猪卵泡闭锁的启动。

关键词：猪GCs凋亡 miR-34a saRNA NORHA

高温胁迫下不同耐热性切花菊光化学活性和能量分配差异

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植物生理学报 2024年第3期60卷 549-560页

作者：李菲王司琦赵艳莉苗润田黄淦李永华河南农业大学风景园林与艺术学院郑州450002 郑州市特色景观植物资源开发与利用重点实验室郑州450002 开封市农林科学研究院河南开封475000

为探究高温胁迫对切花菊叶片光合作用及PSⅡ和PSⅠ功能的影响,本研究对‘威尼斯’(耐热型品种)、‘紫精灵’(中等耐热型品种)、‘凯撒红’(热敏感型品种)3个耐热性不同的品种45℃处理12 h,研究高温胁迫对切花菊生长、光合参数、光合机... 详细信息

为探究高温胁迫对切花菊叶片光合作用及PSⅡ和PSⅠ功能的影响,本研究对‘威尼斯’(耐热型品种)、‘紫精灵’(中等耐热型品种)、‘凯撒红’(热敏感型品种)3个耐热性不同的品种45℃处理12 h,研究高温胁迫对切花菊生长、光合参数、光合机构中光合电子传递链的运转情况以及光合作用相关基因的影响。结果显示,高温胁迫下‘威尼斯’植株仍保持挺立,而‘凯撒红’严重萎蔫,叶片保水能力较差。高温胁迫下‘威尼斯’、‘紫精灵’和‘凯撒红’Pn分别比对照下降了37.31%、114.92%、104.34%,表明在高温胁迫下光合作用能力下降。3种切花菊的OK、OJ相的荧光差异动力学均显示出正L-band和正K-band,表明放氧复合体活性抑制,PSⅡ单位能量连接下调,PSⅡ天线结构被破坏,是造成高温胁迫下光合作用降低的原因之一。高温胁迫下‘威尼斯’通过保持高水平的PSⅡ最大光化学效率(φPo)、将电子传递到电子传递链初级醌受体(QA)下游的其他电子受体概率(ψEo)和低水平的热耗散量子比率(φDo),使更多的能量被PSⅡ反应中心捕获并进入电子传递链,可利用的光化学反应能量多于‘紫精灵’和‘凯撒红’。此外,高温胁迫下,‘威尼斯’捕获的能量传递到电子链末端的量子产率(δRo)降低,表明更多的PSⅠ参与环式电子传递,PSⅠ的活性(ΔI/Io)提高;同时,‘威尼斯’在高温胁迫下可保持较高的Cmrbc L、Cmpsb A、Cmpsa A和Cmpsa B基因表达水平,减轻光抑制,增强光合作用,从而具有更好的耐热性。本研究系统地阐明了高温胁迫下不同耐热性切花菊光化学活性和能量分配差异,表明耐热性不同的切花菊品种有着不同的热胁迫保护机制,为耐热切花菊品种的选育提供理论依据。

关键词：切花菊高温胁迫光化学活性能量分配

Hydrochemistry and H-O-C-S Isotopic Geochemistry Characteristics of Geothermal Water in Nyemo-Nagqu,Tibet

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Acta Geologica Sinica(English Edition) 2017年第2期91卷 644-657页

作者： wang siqi LIU Zhao SHAO Jingli School of Water Resources and Environment China University of GeosciencesBeijing 100083China School of Water Resources&Environment Hebei GEO UniversityShijiazhuang 050031China Hebei Province Key Laboratory of Sustained Utilization&Development of Water Resources Shijiazhuang 050031China

Nyemo-Nagqu, Tibet, is rich in high-temperature geothermal resources. The geothermal fields in Yangbajain and Yangyi as well as 11 unexplored geothermal fields along the geothermal belt from Nyemo to Nagqu were systematically investigated and the hydrochemistry data were collected from the whole field. Meanwhile, H-O-C-S isotope data were obtained for the new fields, and H-O isotope data for the Yangbajain and Yangyi fields. A comparison of the Nyemo-Nagqu geothermal fields with those in the Yangbajain area shows that the types of high-temperature geothermal water are dominated by Cl-Na and Cl·HCO3-Na, while the types of medium-high-temperature geothermal water are dominated by HCO3-Na. The concentrations of Li, F, SiO2, and HBO2 in the geothermal water are positively correlated with Cl content, indicating possible mixing with magma water. The reservoir temperatures range from 90 to 270°C by geothermometers. Slight drifting of 18O was recorded at the Dongweng and Nyingzhong high-temperature geothermal fields, while more significant drifting was recorded at Gulu. The geothermal water is mainly replenished by atmospheric precipitation. The low tritium contents（〈1 TU） of the geothermal water from Nyingzhong, Gulu, and Luoma indicate that it is mainly replenished by sub-modern（prior to 1952） water, while the high tritium content（8.4 TU） in Yuela implies modern water replenishment. Other geothermal fields are replenished by a mix of sub-modern fresher water. The isotopic data in this study show that the carbon and sulfur in the geothermal water originates mainly from sediment leaching, with some of the carbon and sulfur having a deep origin.

关键词： geothermal water hydrochemical characteristics isotope data Tibet Proto-Tethys

中国共产党跳出历史周期率“两个答案”的探索历程、辩证关系与协同路向

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中共青岛市委党校青岛行政学院学报 2024年第2期 5-10页

作者：任志江王思琪南开大学马克思主义学院天津300071 西南财经大学马克思主义学院四川成都611130

“人民监督”和“自我革命”,是我们党持续探索破解历史周期率难题所取得的理论结晶与实践成果,深化了对中国共产党长期执政规律的认识。这“两个答案”之间存在着既相互联系、内在统一,又相互区别、各有侧重的辩证关系。一方面,在践行... 详细信息

“人民监督”和“自我革命”,是我们党持续探索破解历史周期率难题所取得的理论结晶与实践成果,深化了对中国共产党长期执政规律的认识。这“两个答案”之间存在着既相互联系、内在统一,又相互区别、各有侧重的辩证关系。一方面,在践行人民至上的价值理念、坚持党的领导地位、实现党的长期执政上环环相扣,具有高度的内在统一性;另一方面,在各自产生的时代背景、面临的历史任务、选择的解题角度、推进的实现方法上都存在着显著的差异。在新的“赶考”路上,我们要通过推进“两个答案”紧密结合、协同发力,来实现党长期科学执政、民主执政、依法执政。

关键词：历史周期率两个答案人民监督自我革命辩证关系

乡村振兴语境下农村社会工作研究热点与趋势的可视化分析

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黄冈职业技术学院学报 2024年第3期26卷 63-69页

作者：东波王思琦陈宏雷东北石油大学黑龙江大庆163318 大兴安岭地区信访局黑龙江大兴安岭165000 哈尔滨医科大学大庆校区黑龙江大庆163318

随着乡村振兴战略的推进,农村成为社会工作彰显服务社会专业优势的重要场域。伴随农村社会工作实践的开展,理论界掀起了农村社会工作参与乡村振兴的研究热潮。运用CiteSpace文献计量分析软件,对中国知网2007年1月至2023年12月间农村社... 详细信息

随着乡村振兴战略的推进,农村成为社会工作彰显服务社会专业优势的重要场域。伴随农村社会工作实践的开展,理论界掀起了农村社会工作参与乡村振兴的研究热潮。运用CiteSpace文献计量分析软件,对中国知网2007年1月至2023年12月间农村社会工作的核心期刊文献进行可视化分析,结果表明,农村社会工作与精准脱贫、乡村振兴、社会事业相关内容的研究是热点研究问题。同时发现农村社会工作的研究以学理分析为主,运用社会工作方法介入农村社会工作问题的实证研究有限,尚没有形成跨领域及交叉学科的研究氛围。加强理论与实践有机结合的专门研究以及跨学科的合作是未来研究的发展趋势。

关键词：农村社会工作文献计量 CiteSpace软件知识图谱热点分析

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磁性炭基菌球降解土壤阿特拉津性能的研究

农业环境科学学报 2024年第5期43卷 1067-1076页

作者：魏书奇李梓玮吴志欢毕馥漩王思琪孟庆娟东北农业大学资源与环境学院哈尔滨150010

本研究通过海藻酸钠包埋磁性生物炭与降解菌DNS32形成磁性炭基菌球(DMBC-P),并将其用于阿特拉津(ATZ)污染土壤的修复,探讨其去除ATZ的效能及促进大豆幼苗生长的能力。研究表明,当海藻酸钠与氯化钙的浓度为2%时,DMBC-P对ATZ的去除能力最... 详细信息

本研究通过海藻酸钠包埋磁性生物炭与降解菌DNS32形成磁性炭基菌球(DMBC-P),并将其用于阿特拉津(ATZ)污染土壤的修复,探讨其去除ATZ的效能及促进大豆幼苗生长的能力。研究表明,当海藻酸钠与氯化钙的浓度为2%时,DMBC-P对ATZ的去除能力最强。在DMBC-P投加量为2%、温度为30℃、pH=7.3时,其对水体中ATZ的去除率可达到99.99%;并且在pH为3.3~7.3、温度为10~50℃以及ATZ浓度为30~140 mg·L^(-1)的环境中,DMBC-P对ATZ的去除性能仍然十分优异且其可以被有效回收。盆栽试验结果表明,施用DMBC-P进行修复后,该处理下大豆幼苗的生理指标显著高于空白对照处理,叶绿素a、叶绿素b、类胡萝卜素和总叶绿素含量分别提高79.14%、45.48%、67.87%和110.78%。研究表明,DMBC-P施用于污染土壤中能够实现ATZ的高效去除和材料有效回收,是一种极具潜力的污染土壤修复材料。

关键词：阿特拉津 DNS32降解菌磁性生物炭固定化技术土壤修复

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Modeling and flapping vibration suppression of a novel tailless flapping wing micro air vehicle

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Chinese Journal of Aeronautics 2022年第3期35卷 309-328页

作者： siqi wang Bifeng SONG Ang CHEN Qiang FU Jin CUI School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518057China School of Aeronautic Science and Engineering Beihang UniversityBeijing 100083China

This paper establishes and analyzes a high-fidelity nonlinear time-periodic dynamic model and the corresponding state observer for flapping vibration suppression of a novel tailless Flapping Wing Micro Air Vehicle(FWMAV),named ***,a complete modeling of NPU-Tinybird is determined,including the aerodynamic model based on the quasi-steady method,the kinematic and dynamic model about the mechanism of flapping and attitude control,combined with the single rigid body dynamic *** on this,a linearized longitudinal pitch dynamic cycle-averaged model is obtained and analyzed through the methods of neural network fitting and system identification,preparing for the design of flapping vibration suppression *** vibration is an inherent property of the tailless FWMAV,which arises from the influence of time-periodic aerodynamic forces and *** can be captured by attitude and position sensors on the plane,which impairs the flight performance and efficiency of flight controller and *** deal with this problem,a novel state observer for flapping vibration suppression is designed.A robust optimal controller based on the linear quadratic theory is also designed to stabilize the closed-loop *** results are given to verify the performance of the observer,including the closed loop responses combined with robust optimal controller,the comparison of different parameters of observer and the comparison with several classic methods,such as Kalman filter,H-infinity filter and low-pass filter,which prove that the novel observer owns a fairly good suppression effect on flapping vibration and benefits for the improvement of flight performance and control efficiency.

关键词： Flapping vibration Flapping wing Linear quadratic theory Robust control State observer

Effect of Sr^(2+)on 3D gel-printed Sr_(3-x)Mg_(x)(PO_(4))_(2)composite scaffolds for bone tissue engineering

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International Journal of Minerals,Metallurgy and Materials 2023年第11期30卷 2236-2244页

作者： Hongyuan Liu Jialei Wu siqi wang Jing Duan Huiping Shao Institute for Advanced Materials and Technology University of Science and Technology BeijingBeijing 100083China

Porous magnesium strontium phosphate(Sr_(3-x)Mg_(x)(PO_(4))_(2))(x=2,2.5,3)composite scaffolds were successfully prepared by three dimension gel-printing(3DGP)method in this *** results show that Sr_(0.5)Mg_(2.5)(PO_(4))_(2)scaffolds had good compressive strength,and Sr_(1.0)Mg_(2.0)(PO_(4))_(2)scaffolds had good degradation rate in *** weight loss rate of Sr_(1.0)Mg_(2.0)(PO_(4))_(2)scaffolds soaked in simulated body fluid(SBF)or 6 weeks was 6.96%,and pH value varied between 7.50 and 8.61,which was within the acceptable range of human *** biological experiment shows that MC3T3-E1 cells had good adhesion and proliferation on the surface of Sr_(3-x)Mg_(x)(PO_(4))_(2)*** with pure Mg3(PO_(4))_(2)scaffolds,strontium doped scaffolds had excellent comprehensive properties,which explain that Sr_(3-x)Mg_(x)(PO_(4))_(2)composite scaffolds can be used for bone tissue engineering.

关键词： 3D printing magnesium phosphatase strontium porous scaffolds degradability

Advances in research on Shadoo, shadow of prion protein

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Chinese Science Bulletin 2014年第9期59卷 821-827页

作者： siqi wang Hui Zhao Yaping Zhang Laboratory for Conservation and Utilization of Bio-resource Yunnan University State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences

Prion plays a central role in the pathogenesis of transmissible spongiform encephalopathies,also known as prion ***,the biology of the protein and the pathophysiology of these diseases remain largely *** has been speculated that additional factor or factors may be involved in the pathogenesis of prion ***,a PrP-like protein,recognized as shadow of prion protein(Shadoo,Sho),is thought to be an interesting candidate factor because both the prion protein and Sho have been shown to have overlapping expression patterns and shared ***,extensive study of Sho may advance our understanding of the enigmatical biology of prion and prion *** this review,recent studies on Sho associated with prion diseases and functions are *** studies have demonstrated the functional importance of Sho,and they further need to investigate its biological roles in prion diseases.

关键词：阴影朊蛋白传染性海绵状脑病生物学作用朊病毒病朊病毒蛋白朊病毒疾病发病机制