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Antisense Oligonucleotide Targeting TGF-β1 Abrogates Tumorigenicity of Rhabdomyosarcoma in vivo

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Chinese Journal of Clinical Oncology 2008年第4期5卷 258-262页

作者： shouli wang Huihua Yao Lingling Guo Liang Dong Shigang Li Haizhen Deng Maomin Sun Department of Pathology Medical School of Soochow University Suzhou 215123 Jiangsu Province China. Department of Surgery the Second Hospital of Soochow University Suzhou 215123 Jiangsu Province China. Department of Anatomy Medical School of Soochow University Suzhou 215123 Jiangsu Province. China.

OBJECTIVE Over-expression of transforming growth factor β1 （TGF-β1） has been observed in many advanced cancers. The present study was aimed at developing potential antisense oligonucleotides （ASONs） to repress TGF-β1 expression in rhabdomyosarcoma （RMS） RD cells, and to examine their effect on tumorigenicity of RD cells in vivo. METHODS ASONs targeting the region surrounding the start codon of TGF-β1 were synthesized and transferred into cells in the form of complexes with Lipofectamine 2000. The TGF-β1 protein was determined by immunofluorescence and ELISA. The cell viability and cell cycle were examined by MTT and flow cytometry. The RD cells, with or without TGF-β1ASON, in 50 μl of serum-free EMDM medium were injected subcutaneously into the right flank of nude mice. The tumors were then measured and weighed. RESULTS The ASON sequence targeting the first start site at bases 841-855 of the human TGF-β1 gene had the greatest effect on attenuating the expression of TGF-β1 （P 〈 0.05）. The ASONs induced a decrease in OD values after 6 d （P 〈 0.05）. Analysis of the cell cycle revealed that the ASON induced a significant decrease in cells in the S phase and an increase in cells in the G1 phase （P 〈 0,05）. In the nude mice model, the mean tumor volume, after 2 weeks of treatment with Lipofectamine or ASON, decreased to 88.5% or 55% respectively, compared to the control tumor size, resulting in a significant difference （P 〈 0.01）. CONCLUSION The sequence of the ASON, which targeted the start condon at the bases 841-855 of the human TGF-β1 gene, was demonstrated to be a useful agent for studying the regulation of TGF-β1 over-expression in RD cells, and has important therapeutic potential for suppressing the tumorigenicity of human RMS in vivo.

关键词： TGF-β1 antisense oligonucleotides rhabdomyosarcoma tumorigenicity.

Inhibition of Differentiation by Transforming Growth Factor β1 in Rhabdomyosarcoma Cells

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Chinese Journal of Clinical Oncology 2007年第5期4卷 327-332页

作者： shouli wang Huihua Yao Zhenghong Qin Shigang Li Yizhong Feng Xiuzhen wang Min Deng Lingling Guo Lifeng Zhang Molecular Pathology Laboratory Medical School of Soochow University Suzhou 215123 China Department of Surgery the Second Affiliated Hospital of Soochow University Suzhou 215123 China. Pharmacology Laboratory Medical School of Soochow University Suzhou 215123 China

OBJECTIVE To study the effect of transforming growth factor β1 （TGF-β1） on differentiation of rhabdomyosarcoma （RMS） cells METHODS RD （human embryonal RMS cell line） cells, cultured in differentiation medium containing 9-cis retinoic acid （9CRA）, were treated with TGF-β1. Proliferation of the cells was examined by the MTT assay. The differentiation specific proteins （sarcomeric actin and MyHC） and myogenic transcription factors （MyoD1 and myogenin） in the RD cells were assessed by immunofluorescence staining. RESULTS Compared to control cells, treatment with lower concentrations of TGF-β1 （0.1 and 0.2 ng/ml） induced an increase in OD values after 4 d （P〈0.01）, whereas higher concentrations of TGF-β1 （2 and 5 ng/ml） led to a reduction of cell viability （P〈0.01）. After exposure to 9CRA, the viability of the cells decreased significantly （P〈0.01）, while sarcomeric actin, MyHC and myogenin were induced. These changes were antagonized by TGF-β1 （0.1 ng/ml）. No changes were observed in expression of MyoD1. CONCLUSION The RMS cells, derived from myogenic progenitors are committed to a myogenic fate, but are arrested in the differentiation course by the addition of TGF-β1 which represses some of the myogenic transcription factors.

关键词： TGF-β1 proliferation differentiation rhabdomyosarcoma.

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上海城市道路窨井防沉降技术应用探讨

给水排水 2010年第2期36卷 94-97页

作者：王守立中铁十八局集团第五工程有限公司天津300450

城市道路窨井沉降现象比较普遍,对行车舒适度、安全产生不利影响,并带来噪声污染,增加相应的维护费用。从设计和施工方面分析了窨井沉降的原因。对"大盖板"设计、可调高设计和ACO自调式窨井等三种防沉降技术进行介绍和探讨,并对这三种... 详细信息

城市道路窨井沉降现象比较普遍,对行车舒适度、安全产生不利影响,并带来噪声污染,增加相应的维护费用。从设计和施工方面分析了窨井沉降的原因。对"大盖板"设计、可调高设计和ACO自调式窨井等三种防沉降技术进行介绍和探讨,并对这三种技术在上海市的应用推广情况作了介绍。

关键词：城市道路窨井沉降防沉降技术 ACO自调式窨井上海市

Characterization of dendritic cell subtypes in human cord blood by single-cell sequencing

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Biophysics Reports 2019年第4期5卷 199-208页

作者： Xiaoyang Jin Lingyuan Meng Zhao Yin Haisheng Yu Linnan Zhang Weifeng Liang shouli wang Guanyuan Liu Liguo Zhang Key Laboratory of Immunity and Infection Institute of Biophysics Chinese Academy of Sciences Department of Cardiology 306th Hospital of PLA University of Chinese Academy of Sciences Department of Gynecology and Obstetrics Beijing Chaoyang Hospital Capital Medical University Key Laboratory of Human Disease Comparative Medicine and Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Institute of Laboratory Animal Science (ILAS) Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical Collage (PUMC)

Dendritic cells(DCs) are professional antigen-presenting cells(APCs). The key functions of DCs include engulfing, processing and presenting antigens to T cells and regulating the activation of T cells. There are two major DC subtypes in human blood: plasmacytoid DCs(p DCs) and conventional DCs. To define the differences between the adult and infant immune systems, especially in terms of DC constitution, we enriched DCs from human cord blood and generated single-cell RNA sequencing data from about 7000 cells using the 10 x Genomics Single Cell 30 Solution. After incorporating the differential expression analysis method in our clustering process, we identified all the known dendritic cell subsets. Interestingly, we also found a group of DCs with gene expression that was a mix of megakaryocytes and p DCs. Further, we verified the expression of selected genes at both the RNA level by PCR and the protein level by flow cytometry. This study further demonstrates the power of single-cell RNA sequencing in dendritic cell research.

关键词： Cord blood Dendritic cells Hematology Immunology Single-cell sequencing

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microRNA与肾细胞癌关系的研究进展

中华病理学杂志 2017年第8期46卷 575-577页

作者：张永胜王守立苏州大学附属第二医院病理科 215004 苏州大学医学部病理系

肾细胞癌（renal cell carcinoma）的发病率约占全身恶性肿瘤的3%,占成人肾脏原发性恶性肿瘤的85%~90%,为泌尿系统第二大恶性肿瘤,其发病率仅次于膀胱癌,死亡率高达40%[1].2014年,美国新发肾脏恶性肿瘤63 920例,死亡13 860例[1].2015年... 详细信息

肾细胞癌（renal cell carcinoma）的发病率约占全身恶性肿瘤的3%,占成人肾脏原发性恶性肿瘤的85%~90%,为泌尿系统第二大恶性肿瘤,其发病率仅次于膀胱癌,死亡率高达40%[1].2014年,美国新发肾脏恶性肿瘤63 920例,死亡13 860例[1].2015年,我国肾细胞癌新发病例数为66 800例（男性43 200例,女性23 600例）,占全部恶性肿瘤新发病例的1.56%,在所有恶性肿瘤发病中排14位[2];肾细胞癌患者死亡例数23 400例（男性15 200例,女性8 200例）,占全部恶性肿瘤死亡例数的0.83%,在所有恶性肿瘤死亡率中排名第17位.常见的肾细胞癌组织学类型分为透明细胞性肾细胞癌、乳头状肾细胞癌、嫌色细胞性肾细胞癌以及Bellini集合管癌.

关键词：乳头状肾细胞癌 microRNA 原发性恶性肿瘤肾脏恶性肿瘤透明细胞性组织学类型肿瘤死亡率发病率

Age, estimated glomerular filtration rate and ejection fraction score predicts contrast-induced acute kidney injury in patients with diabetes and chronic kidney disease： insight from the TRACK-D study

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Chinese Medical Journal 2014年第12期127卷 2332-2336页

作者： Li Jing Li Yi wang Xiaozeng Yang Shuguang Gao Chuanyu Zhang Zheng Yang Chengming Jing Quanming wang shouli Ma Yingyan wang Zulu Liang Yanchun Han Yaling Department of Cardiology Shenyang Northern HospitalShenyangLiaoning 110016China Department of Cardiology Jinan Military 88th HospitalTaianShandong 271000China Department of Cardiology Henan Provincial Peoples HospitalZhengzhouHenan 450003China Department of Cardiology First Affiliated Hospital of Lanzhou UniversityLanzhouGansu 730000China Department of Cardiology Daping Hospital and the Research Institute of Surgery of the Third Military Medical UniversityChongqing 400038China Depal tment of Cardiology The 306th'Hospital of People's LiberationArmy Beijing 10000 China

Background The occurrence of contrast induced acute kidney injury （CIAKI） has a pronounced impact on morbidity and *** aim of the present study was to appraise the diagnostic efficacy of age,estimated glomerular filtration rate （eGFR） and ejection fraction （AGEF） score （age/EF（％）＋1 （if eGFR was ＜60 ml·min-1·1.73 m2）） as an predictor of CIAKI in patients with diabetes mellitus （DM） and concomitant chronic kidney disease （CKD）.Methods The AGEF score was calculated for 2 998 patients with type 2 DM and concomitant CKD who had undergone coronary/peripheral arterial *** was defined as an increase in sCr concentration of 0.5 mg/dl （44.2 mmol/L） or 25％ above baseline at 72 hours after exposure to the contrast *** hoc analysis was performed by stratifying the rate of CIAKI according to AGEF score *** diagnostic efficacy of the AGEF score for predicting CIAKI was evaluated with receiver operating characteristic （ROC） *** The AGEF score ranged from 0.49 to *** AGEF score tertiles were defined as follows：AGEFlow ≤0.92 （n=1 006）; 0.92 ＜AGEFmid ≤1.16 （n=1 000）,and ACEFhigh ＞1.16 （n=992）.The incidence of CIAKI was significantly different in patients with low,middle and high AGEF scores （AGEFlow=1.1％,AGEFmid=2.3％ and AGEFhigh=5.8％,P ＜0.001）.By multivariate analysis,AGEF score was an independent predictor of CIAKI （odds ratio=4.96,95％ CI：2.32-10.58,P ＜0.01）.ROC analysis showed that the area under the curve was 0.70 （95％ CI：0.648-0.753,P ＜0.001）.Conclusion The AGEF score is effective for stratifying risk of CIAKI in patients with DM and CKD undergoing coronary/peripheral arterial angiography.

关键词： AGEF score contrast induced acute kidney injury diabetes chronic kidney disease

E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells

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Biophysics Reports 2015年第3期1卷 139-147页

作者： Jianping Ma Haisheng Yu Xiangyun Yin Menglan Cheng Quanxing Shi Zhao Yin Xiaohua Nie wang shouli Liguo Zhang Key Laboratory of Infection and Immunity Institute of Biophysics Chinese Academy of Sciences University of Chinese Academy of Sciences Department of Cardiology 306th Hospital of PLA

Plasmacytoid dendritic cells(pDCs) play important roles in initiating and regulating immune *** infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progression. However,a specific antibody for identifying pDCs by immunohistochemical staining on paraffin-embedded tissue sections is still lacking. Here, we developed a novel antibody targeted E2-2, a transcription factor preferentially expressed in pDCs. The antibody stains the nuclei of pDCs specifically in immunohistochemical analysis of various tissues from both human and rhesus monkey. This novel antibody will serve as a beneficial tool for pDC-related basic research and clinical investigation.

关键词： E2-2 Immunohistochemistry Monoclonal antibody pDC

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蕴藻浜大桥下承式斜靠钢箱拱肋施工技术

国防交通工程与技术 2017年第1期15卷 57-60页

作者：王守立中铁十八局集团第五工程有限公司天津300450

大型桥梁在城市内河重要航道上的施工,首先要满足安全通航的常态要求。在蕴藻浜大桥的施工中,通过方案优化设计,采取先梁后拱和先拱后梁相结合的施工方法,在满足通航要求的前提下,最大程度的减少了深水工作量,缩短了水上施工周期,整个... 详细信息

大型桥梁在城市内河重要航道上的施工,首先要满足安全通航的常态要求。在蕴藻浜大桥的施工中,通过方案优化设计,采取先梁后拱和先拱后梁相结合的施工方法,在满足通航要求的前提下,最大程度的减少了深水工作量,缩短了水上施工周期,整个过程安全、可靠、精确,同时有效地降低了施工成本。

关键词：内河航道大跨度系杆拱肋斜靠拱

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空间索面斜拉索张拉方法研究

市政技术 2016年第6期34卷 53-56页

作者：王守立中铁十八局集团第五工程有限公司天津300450

蕴藻浜斜拉桥的主跨斜拉索为双排单索面,边跨斜拉索为为空间扭转双索面,这种不对称的布置方式使得斜拉索在张拉过程中相互影响,每一次的张拉及索力调整均会导致其他斜拉索索力的二次分布。因此斜拉索的张拉顺序、张拉力的大小对桥梁最... 详细信息

蕴藻浜斜拉桥的主跨斜拉索为双排单索面,边跨斜拉索为为空间扭转双索面,这种不对称的布置方式使得斜拉索在张拉过程中相互影响,每一次的张拉及索力调整均会导致其他斜拉索索力的二次分布。因此斜拉索的张拉顺序、张拉力的大小对桥梁最终的成桥状态会产生较大的影响。在施工过程中,通过对斜拉索张拉方法进行仔细研究,确保了桥梁成桥时的受力状态与设计的理想状态相符。

关键词：斜拉桥彩针形独塔空间扭转双面索张拉调索