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Grain & Oil Science and Technology 2024年第3期7卷 159-167页

作者： meiyue wang Guanhao Bu Yufei Xing Mengke Ren Yang wang Yijing Xie College of Food Science and Engineering Henan University of TechnologyZhengzhou 450001China

Soybean protein has high nutritional value, but its functional properties are easily affected by external factors,which limits its application in food industry. In the study, soybean protein isolate(SPI) was modified by dry heat glycation of galactooligosaccharides(GOS). The gel properties, antioxidant properties and structural changes of SPI-GOS conjugates were investigated. The application of SPI-GOS conjugates in noodles was also explored. The results observed that the glycation degree of SPI increased with the increasing reaction time. SDS-PAGE and spectral analysis showed the changes of spatial conformation of SPI after glycation. The antioxidant activity of SPI increased after glycation and DPPH radical scavenging activity of SPI-GOS peaked at 48 h of reaction. The hardness, elasticity and resilience of soybean protein gel reached their relative maximum at 48 h, 48 h and 12 h of glycation reaction, respectively. Moreover, the appropriate addition of glycated SPI improved the quality of noodles. The noodles with 4% addition of SPI-GOS had higher hardness, elasticity and tensile properties. This study will provide an effective method to modify soybean protein and expand the use of soybean protein in food industry.

关键词： Soy protein isolate Glycation Galactooligosaccharide Functional properties Noodles

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蛋白多肽美拉德反应物稳定乳液的研究进展

食品科学 2024年第3期45卷 227-234页

作者：王美月布冠好常永锋赵晓玲河南工业大学粮油食品学院河南郑州450001

乳液被认为是一种用于递送生物活性物质的良好体系,但由于其不稳定,极大地限制了应用。蛋白多肽具有独特的营养价值和生物活性,其结构灵活且易于在油水界面展开和重排,因此蛋白多肽稳定的乳液受到了广泛关注。蛋白多肽作为乳化剂虽然能... 详细信息

乳液被认为是一种用于递送生物活性物质的良好体系,但由于其不稳定,极大地限制了应用。蛋白多肽具有独特的营养价值和生物活性,其结构灵活且易于在油水界面展开和重排,因此蛋白多肽稳定的乳液受到了广泛关注。蛋白多肽作为乳化剂虽然能够形成有效的乳液,但在稳定乳液方面效率较低。有研究发现蛋白多肽美拉德反应物稳定的乳液具有较好的稳定性、理化特性和功能特性。因此,本文对蛋白多肽美拉德反应物稳定乳液的作用机制、理化特性、功能特性、影响乳液稳定性的因素及其在递送体系中的应用进行了详细的阐述,为扩大蛋白多肽乳液在递送生物活性物质中的应用提供理论依据。

关键词：蛋白多肽乳液美拉德反应稳定性递送体系

3D bioprinting for cell culture and tissue fabrication

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Bio-Design and Manufacturing 2018年第1期1卷 45-61页

作者： Honglei Jian meiyue wang Shengtao wang Anhe wang Shuo Bai State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China

Three-dimensional （3D） bioprinting is a computer-assisted technology which precisely controls spatial position of biomaterials, growth factors and living cells, offering unprecedented possibility to bridge the gap between structurally mimic tissue constructs and functional tissues or organoids. We briefly focus on diverse bioinks used in the recent progresses of biofabrication and 3D bioprinting of various tissue architectures including blood vessel, bone, cartilage, skin, heart, liver and nerve systems. This paper provides readers a guideline with the conjunction between bioinks and the targeted tissue or organ types in structuration and final functionalization of these tissue analogues. The challenges and perspectives in 3D bioprinting field are also illustrated.

关键词： 3D bioprinting Bioink Cell culture Tissue fabrication Organoid

非均质孔隙介质径向溶质双峰反常运移数值模拟研究

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安全与环境工程 2024年第3期31卷 225-234页

作者：左孔辉李旭朱棋尹茂生张海涛王美悦安徽理工大学地球与环境学院安徽淮南232001 中国地质大学(武汉)环境学院湖北武汉430078 南方科技大学环境学院广东深圳518055

为了探究孔隙含水层的非均质性对注水井附近溶质运移的控制机理以及溶质羽的空间分布特征,基于美国MADE场地含水层地质统计学数据,利用T-PROGS(transition probability geostatistical software)构建了三维径向非均质随机模型;并结合MOD... 详细信息

为了探究孔隙含水层的非均质性对注水井附近溶质运移的控制机理以及溶质羽的空间分布特征,基于美国MADE场地含水层地质统计学数据,利用T-PROGS(transition probability geostatistical software)构建了三维径向非均质随机模型;并结合MODFLOW和MT3DMS模块模拟了注水井附近的溶质运移过程,研究了沉积相不同空间结构对溶质运移过程的影响。结果表明:在非均质含水层注水井附近普遍存在双峰反常运移现象;径向溶质运移受高渗透性沉积相的沉积长度和厚度影响较大,沉积长度越大溶质质量空间分布曲线双峰现象越明显,尤其当沉积长度扩大到1.75倍时,双峰现象最明显;通过模拟结果可以得出高渗透性沉积相所在位置与高流速优先流所处位置呈一定的正相关性。总体而言,高渗透性的含水介质在冲洪积孔隙含水层中具有很强的连通性,易形成优先水流通道,导致溶质沿快速和慢速两种通道运移,从而使得穿透曲线出现双峰现象。

关键词：非均质含水层溶质运移双峰分布数值模拟

酶解联合糖基化对花生蛋白结构及功能特性的影响

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食品研究与开发 2024年第14期45卷 8-15页

作者：王美月布冠好谢一敬汪眻河南工业大学粮油食品学院河南郑州450001

利用木瓜蛋白酶有限酶解花生分离蛋白,再与葡聚糖在80℃下进行湿法糖基化反应2~6 h,研究糖基化对花生分离蛋白酶解物结构及功能特性的影响。结果表明,糖基化反应后花生分离蛋白酶解物具有较高的接枝度,紫外光谱和内源荧光光谱表明其空... 详细信息

利用木瓜蛋白酶有限酶解花生分离蛋白,再与葡聚糖在80℃下进行湿法糖基化反应2~6 h,研究糖基化对花生分离蛋白酶解物结构及功能特性的影响。结果表明,糖基化反应后花生分离蛋白酶解物具有较高的接枝度,紫外光谱和内源荧光光谱表明其空间结构发生改变,傅里叶变换红外光谱结果证实花生分离蛋白酶解物与葡聚糖发生糖基化反应。通过对复合改性后花生分离蛋白功能特性进行评价,发现花生分离蛋白的溶解度在pH3~7内均有所改善,反应4 h的糖基化产物在pH7时溶解度达到最高,为91.56%,较相同pH值下的花生分离蛋白提高1.77倍。经过4 h的接枝反应后花生分离蛋白酶解物的乳化活性最高,达到61.08 m^(2)/g,较花生分离蛋白提高5.28倍。此外,有限酶解和糖基化反应能够明显降低花生分离蛋白的表面疏水性、提高花生分离蛋白的抗氧化活性。因此,通过酶解和糖基化复合改性能够有效改善花生蛋白的功能特性,扩大其应用范围。

关键词：花生分离蛋白酶解糖基化葡聚糖结构特性功能特性

CSCS:a chromatin state interface for Chinese Spring bread wheat

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aBIOTECH 2021年第4期2卷 357-364页

作者： Xiaojuan Ran Tengfei Tang meiyue wang Luhuan Ye Yili Zhuang Fei Zhao Yijing Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences300 Fenglin RoadShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China School of Life Science Henan UniversityKaifeng 457000HenanChina

A chromosome-level genome assembly of the bread wheat variety Chinese Spring(CS)has recently been ***-wide identification of regulatory elements(REs)responsible for regulating gene activity is key to further mechanistic *** epigenetic activity can reflect RE activity,defining chromatin states based on epigenomic features is an effective way to detect ***,we present the web-based platform Chinese Spring chromatin state(CSCS),which provides CS chromatin signature *** indudes 15 recently published epigenomic data sets including open chromatin and major chromatin marks,which are further partitioned into 15 distinct chromatin *** curates detailed information about these chromatin states,with trained self-organization mapping(SOM)for segments in all chromatin states and JBrowse visualization for genomic regions or *** analysis for genomic regions or genes,GO analysis for genes and SOM analysis for new epige-nomic data sets are also integrated into *** summary,the CSCS database contains the combina-torial patterns of chromatin signatures in wheat and facilitates the detection of functi onal elements and further clarification of regulatory *** illustrate how CSCS enables biological insights using one example,demonstrating that CSCS is a highly useful resource for intensive data *** is available at http://***/CSCS/.

关键词： Bread wheat Chinese Spring Chromatin state Epigenetics Database

Wheat-RegNet:An encyclopedia of common wheat hierarchical regulatory networks

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Molecular Plant 2023年第2期16卷 318-321页

作者： Tengfei Tang Shilong Tian Haoyu wang Xiaotong Lv Yilin Xie Jinyi Liu meiyue wang Fei Zhao Wenli Zhang Hao Li Yijing Zhang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesCollege of AgricultureHenan UniversityKaifengHenan 457004China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences300 Fenglin RoadShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China State Key Laboratory for Crop Genetics and Germplasm Enhancement CICMCPNanjing Agricultural UniversityNo.1 WeigangNanjingJiangsu 210095 China State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and DevelopmentDepartment of BiochemistryInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China China Center of Industrial Culture Collection(CICC) China National Research Institute of Food and Fermentation IndustriesBeijing 100015China

Dear Editor,Common wheat(Triticum aestivum,2n=6×=42,AABBDD)is the staple crop *** the gene regulatory network provides essential information for mechanism studies and targeted manipulation of gene activity for ***,it is a challenging task given the extremely large(16 Gb)and complicated allohexaploid genome of common *** multi-omics data is a compelling approach to construct the hierarchical regulatory network.

关键词： aestivum Wheat hierarchical

Biomimetic,biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering

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Regenerative Biomaterials 2023年第1期10卷 1222-1232页

作者： Runying Guo Rui Zhang Sirui Liu Yanyu Yang Wenhang Dong meiyue wang Hongyan Mi Mengzhe Liu Jingjing Sun Xue Zhang Yimeng Su Yiming Liu Di Huang Rui Li Department of Stomatology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000PR China Department of Stomatology The First Affiliated Hospital of Nanchang UniversityNanchang 330000PR China Oral and Maxillofacial Department Zhengzhou Stomatology HospitalZhengzhou 450000PR China College of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450000PR China Research Center for Nano-biomaterials and Regenerative Medicine College of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024PR China

It is still a huge challenge for bone regenerative biomaterial to balance its mechanical,biological and biodegradable *** the present study,a new composite material including treated dentin matrix(TDM)andα-calcium sulphate hemihydrate(α-CSH)was *** optimal composition ratio between TDM andα-CSH was *** results indicate that both components were physically mixed and structurally *** compressive strength reaches up to 5.027±0.035 MPa for 50%TDM/α-CSH group,similar to human cancellous bone *** experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins(ALP,RUNX2,OPN)is significantly *** vivo experiments suggest that the addition of TDM for each group(10%,30%,50%)effectively promotes cell proliferation and *** addition,50%of the TDM/α-CSH combination displays optimal *** novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering.

关键词： bone defect treated dentin matrix α-calcium sulphate hemihydrate osteogenesis

Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling

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Bioactive Materials 2022年第1期7卷 85-97页

作者： Sirui Liu Jingjing Sun Shuai Yuan Yanyu Yang Yuping Gong Ying wang Runying Guo Xue Zhang Yiming Liu Hongyan Mi meiyue wang Mengzhe Liu Rui Li Department of Stomatology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Academy of Medical Sciences at Zhengzhou University ZhengzhouChina College of Materials Science and Engineering Zhengzhou University ZhengzhouHenan 450001China

Treated dentin matrix(TDM)is an ideal scaffold material containing multiple extracellular matrix *** canonical Wnt signaling pathway is necessary for tooth ***,this study investigated whether the TDM can promote the odontogenic differentiation of human dental pulp stem cells(hDPSCs)and determined the potential role of Wnt/β-catenin signaling in this *** concentrations of TDM promoted the dental differentiation of the hDPSCs and meanwhile,the expression of GSK3βwas *** note,the expression of the Wnt/β-catenin pathway-related genes changed significantly in the context of TDM induction,as per RNA sequencing(RNA seq)*** addition,the experiment showed that new dentin was visible in rat mandible cultured with TDM,and the thickness was significantly thicker than that of the control *** addition,immunohistochemical staining showed lower GSK3βexpression in new ***,the GSK3βknockdown hDPSCs performed enhanced odotogenesis compared with the control ***,GSK3βoverexpressing could decrease odotogenesis of TDM-induced *** results were confirmed in immunodeficient mice and Wistar *** suggest that TDM promotes odontogenic differentiation of hDPSCs by directly targeting GSK3βand activating the canonical Wnt/β-catenin signaling pathway and provide a theoretical basis for tooth regeneration engineering.

关键词： Dental pulp stem cells Treated dentin matrix GSK3β Odontogenic differentiation Wnt/β-catenin signaling pathway

Role of Thermolysin in Catalytic-Controlled Self-Assembly of Fmoc-Dipeptides

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CCS Chemistry 2020年第4期2卷 317-328页

作者： meiyue wang Qiansen Zhang Honglei Jian Shijie Liu Jieling Li Anhe wang Qianqian Dong Peng Ren Xin Li Shuo Bai State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190 School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049 Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai 200241.

In recent years,short peptide self-assembled materials,prepared under the control of the thermolysin catalyst,have been investigated extensively and shown to acquire various morphologies and functions as building blocks for a wide range of biomaterials and device ***,the role played by thermolysin in this enzymatically triggered peptide self-assembly is still ***,we designed a series of Fmoc-dipeptide amphiphiles to explore the catalytic role of thermolysin.

关键词： peptide self-assembly thermolysin sequence catalytic efficiency