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Food Science and Human Wellness 2024年

作者： Ruiyun Wu Zixin Han Zhenyu wang Pinglan li Nan Shang Institute of Food Science and Technology， Chinese Academy of Agricultural Sciences， Integrated Laboratory of Processing Technology for Chinese Meat and Dish Products， Ministry of Agriculture and Rural Affairs College of Food Science and Nutritional Engineering， China Agricultural University College of Engineering， China Agricultural University

Chondroitin sulfate (CS) is one of the main bioactive compounds in animal cartilage. In this study， the anti-inflammatory activity of sturgeon-derived chondroitin sulfate (SCS) was evaluated in the DSS-induced BALB/c mice model. Orally administration of SCS significantly alleviated the DSS-induced colitis symptoms， including the reduction of crypt depth， inhibition of the abnormal crypt foci formation， down-regulation of the proinflammatory biomarkers (NO， IL-6， IL-1β and TNF-α) and up-regulation of the anti-inflammatory biomarkers (IL-10 and IL-4). The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice， especially the increase of the relative abundance of Ruminococcaceae and Lachnospiracea. This alternation further induced primary bile acids convert into secondary bile acids. With SCS administration， the levels of deoxycholic acid (DCA) and litho cholic acid (LCA) were increased by ～1.5 and ～2.5-fold， respectively. The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway， thereby suppressing the inflammatory response and attenuating IBD in mice. This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.

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Closure Effect ofⅠ+ⅡMixed-mode Crack for EA4T Axle Steel

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Chinese Journal of Mechanical Engineering 2024年第4期37卷 327-341页

作者： Shuancheng wang Bing Yang Shuwei Zhou Jian li Shoune Xiao State Key Laboratory of Rail Transit Vehicle System Southwest Jiaotong UniversityChengdu 610031China Institute of Metal Forming RWTH Aachen University52064 AachenGermany

The crack-closure effect is a crucial factor that affects the crack growth rate and should be considered in simulation analysis and testing.A mixed-mode I+II loading fatigue crack growth test was performed using EA4T axle steel *** variation of the plastic-induced crack closure(PICC)effect and the roughness-induced crack closure(RICC)effect during crack deflection in the mixed-mode is examined in this *** results show that the load perpendicular to the crack propagation direction hinders the slip effect caused by the load parallel to the crack propagation direction under mixed-mode loading,and the crack deflection is an intuitive manifestation of the interaction between the PICC and *** proportion of the RA value change on the crack side caused by contact friction was reduced by the interaction between PICC and *** roughness of the crack surface before and after the crack deflection is different,and the spatial torsion crack surface is formed during the crack propagation *** the increase of the crack length,the roughness of the fracture surface *** the crack deflection process,the PICC value fluctuates around 0.2,and the RICC value is increased to 0.15.

关键词： Crack closure Crack deflection Plasticity-induced closure Roughness-induced closure Interaction mechanism

Advancing CRISPR base editing technology through innovative strategies and ideas

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Science China life Sciences 2024年

作者： Xiongwei Fan Yang Lei liren wang Xiushan Wu Dali li The Center for Heart Development College of Life Science Hunan Normal University Shanghai Frontiers Science Research Base of Genome Editing and Cell Therapy Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life Sciences East China Normal University Guangdong Provincial Key Laboratory of Pathogenesis Targeted Prevention and Treatment of Heart Disease

The innovation of CRISPR/Cas gene editing technology has developed rapidly in recent years. It is widely used in the fields of disease animal model construction, biological breeding, disease diagnosis and screening, gene therapy, cell localization, cell lineage tracking, synthetic biology, information storage, etc. However, developing idealized editors in various fields is still a goal for future development. This article focuses on the development and innovation of non-DSB editors BE and PE in the platform-based CRISPR system. It first explains the application of ideas for improvement such as “substitution”, “combination”, “adaptation”, and “adjustment” in BE and PE development and then catalogues the ingenious inversions and leaps of thought reflected in the innovations made to CRISPR technology. It will then elaborate on the efforts currently being made to develop small editors to solve the problem of AAV overload and summarize the current application status of editors for in vivo gene modification using AAV as a delivery system. Finally, it summarizes the inspiration brought by CRISPR/Cas innovation and assesses future prospects for development of an idealized editor.

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一类潜伏期和染病期均传染的SEIR传染病模型的稳定性研究

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山西师范大学学报（自然科学版） 2016年第3期30卷 12-14页

作者：米晓丽王鑫山西师范大学数学与计算机科学学院山西临汾041000 山西师范大学现代文理学院数学与计算机科学系山西临汾041000

本文讨论了一类含潜伏期传染的SEIRS传染病模型.确定了各类平衡点存在的条件阈值.利用线性化和李亚普诺夫-lasalle不变集的方法,得到了各类平衡点的稳定性结论.

关键词：传染病模型周期解稳定性

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空间自适应有源消声系统研究

家用电器科技 1999年第4期 25-26页

作者：王礼信高琳北京轻工业学院自动化工程系

本文提出一种采用模型参考自适应状态观测器实现空间有源消声控制的方案。计算机仿真及硬件实验表明，该系统跟踪速度快，消声效果显著，并且结构简单，工作稳定可靠，具有良好的应用前景。

关键词：有源消声系统模型参考计算机仿真

Antioxidant peptides from Lateolabrax japonicus to protect against oxidative stress injury in Drosophila melanogaster via biochemical and gut microbiota interaction assays

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Food Science and Human Wellness 2024年

作者： Chen li lichan li Jing Cheng Xu Chen Mohamed A.Farag Xixi Cai Shaoyun wang College of Chemical Engineering， College of Biological Science and Engineering， Fuzhou University Pharmacognosy Department， Faculty of Pharmacy， Cairo University

Identification of natural substances with antioxidant properties is ongoing research for addressing issues related to oxidative stress especially attributed to environmental effects. Our previous study demonstrated that Lateolabrax japonicus peptides （LPH）， rich in Glu， Gly， and hydrophobic amino acids， exhibited remarkable antioxidant activity in vitro， with though its action mechanism yet to be revealed. Therefore， to assess the in vivo antioxidative properties of LPH， we employed H2O2to generate oxidative stress in Drosophila melanogaster model. Results indicated that LPH significantly prolonged the lifespan of Drosophila subjected to oxidative stress mostly mediated via LPH's enhancement of the antioxidant defense system and intestinal functions. Antioxidant effects were manifested by a decrease in malondialdehyde （MDA） levels， elevated superoxide dismutase （SOD）， catalase （CAT）， and glutathione peroxidase （GSH-Px） activities， decreased levels of reactive oxygen species （ROS） in intestinal epithelial cells， and the preservation of intestinal length. LPH effectively controlled the excessive proliferation and differentiation of oxidative stress-induced Drosophila intestinal stem cells. At the gene level， LPH upregulated the expression of antioxidant-related Nrf2 genes while concurrently downregulated mTOR expression level. Furthermore， high-throughput 16S rDNA sequencing revealed that the addition of LPH significantly influenced the diversity and abundance of the intestinal microbiota in H2O2-induced Drosophila. These findings provide a deeper understanding of the antioxidative mechanism of LPH， suggesting its potential applications in food industry and to be assessed using other in vivo oxidative stress models.

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基于神经网络的粒子输运问题高效计算方法

物理学报 2024年第7期73卷 114-123页

作者：马锐垚王鑫李树勇珩上官丹骅北京应用物理与计算数学研究所北京100094 中物院高性能数值模拟软件中心北京100088

蒙特卡罗方法是求解粒子输运问题的有力工具之一,其局限性在于为达到精度要求需模拟大量粒子,计算耗时长,这阻碍了该方法的进一步应用,尤其在需快速响应的情形.本文结合神经网络和若干蒙特卡罗方法基本原理发展了一种计算方法,能够实现... 详细信息

蒙特卡罗方法是求解粒子输运问题的有力工具之一,其局限性在于为达到精度要求需模拟大量粒子,计算耗时长,这阻碍了该方法的进一步应用,尤其在需快速响应的情形.本文结合神经网络和若干蒙特卡罗方法基本原理发展了一种计算方法,能够实现源分布可变,几何、材料和目标计数不变的中子输运问题的快速准确求解.首先,为高效生成用于神经网络训练的数据,利用重要性原理实现在同样模拟次数基础上有效扩充训练数据集容量,在一定程度上克服了使用蒙特卡罗计算获取训练数据耗时长的缺点.进而,基于目标计数是源分布与重要性函数乘积积分的事实,设计了利用神经网络实现快速输运计算的策略.该网络的输入是中子源项,输出是目标计数,在几何、材料和目标计数固定的情况下,该神经网络可重复使用,根据新的源项快速准确得到目标计数.本文所提出方法的原理和框架同样适用于其他种类粒子的同类型输运问题.基于若干基准模型的验证表明,训练得到的神经网络能在不到1 s的时间内得到目标计数,且与蒙特卡罗大样本模拟得到基准结果的平均相对偏差均低于5%.

关键词：蒙特卡罗方法神经网络粒子输运重要性原理

南溪长江大桥隧道锚原位模型试验及参数反演分析

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科学技术与工程 2015年第4期35卷 160-165页

作者：于新华王丽新付环宇梅松华中国电建集团中南勘测设计研究院有限公司水能资源利用关键技术湖南省重点实验室长沙410014

针对南溪长江大桥南岸隧道锚围岩类别为Ⅳ类和Ⅴ类,其承载力和稳定性相对较低的特点,为了解锚塞体与不同地质条件的围岩相互作用的变形情况,开展了缩尺比例为1/30和1/20的锚塞体原位模型试验。试验成果表明,1/30锚塞体模型试验最高应力... 详细信息

针对南溪长江大桥南岸隧道锚围岩类别为Ⅳ类和Ⅴ类,其承载力和稳定性相对较低的特点,为了解锚塞体与不同地质条件的围岩相互作用的变形情况,开展了缩尺比例为1/30和1/20的锚塞体原位模型试验。试验成果表明,1/30锚塞体模型试验最高应力达到14.24 MPa,为设计应力的28倍,1/20锚塞体模型试验最高应力达到8.68 MPa,为设计应力的17倍。加载过程中两个模型的位移应力曲线基本保持线性,但卸载后残余变形较大。通过有限元方法对试验过程进行了反演,得到的岩体力学参数与试验结果接近,变形规律基本一致。

关键词：桥梁工程隧道锚锚塞体原位模型试验参数反演

The marriage of immunomodulatory,angiogenic,and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scafold for military medicine

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Military Medical Research 2024年第3期11卷 323-344页

作者： Ping Wu lin Shen Hui-Fan liu Xiang-Hui Zou Juan Zhao Yu Huang Yu-Fan Zhu Zhao-Yu li Chao Xu li-Hua Luo Zhi-Qiang Luo Min-Hao Wu lin Cai Xiao-Kun li Zhou-Guang wang Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research the Fifth Affiliated Hospital of Wenzhou Medical UniversityLishui 323000ZhejiangChina Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325000ZhejiangChina Department of Spine Surgery and Musculoskeletal Tumor Zhongnan Hospital of Wuhan UniversityWuhan 430071China National Engineering Research Center for Nanomedicine College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Department of Overseas Education College Jimei UniversityXiamen 361021FujianChina School and Hospital of Stomatology Wenzhou Medical UniversityWenzhou 325035ZhejiangChina

Background:Most bone-related injuries to grassroots troops are caused by training or accidental *** establish preventive measures to reduce all kinds of trauma and improve the combat effectiveness of grassroots troops,it is imperative to develop new strategies and scafolds to promote bone ***:In this study,a porous piezoelectric hydrogel bone scafold was fabricated by incorporating polydopamine(PDA)-modified ceramic hydroxyapatite(PDA-hydroxyapatite,PHA)and PDA-modified barium titanate(PDABaTiO_(3),PBT)nanoparticles into a chitosan/gelatin(Cs/Gel)*** physical and chemical properties of the Cs/Gel/PHA scafold with 0–10 wt%PBT were *** and animal experiments were performed to characterize the immunomodulatory,angiogenic,and osteogenic capabilities of the piezoelectric hydrogel scafold in vitro and in ***:The incorporation of BaTiO_(3) into the scafold improved its mechanical properties and increased self-generated *** to their endogenous piezoelectric stimulation and bioactive constituents,the prepared Cs/Gel/PHA/PBT hydrogels exhibited cytocompatibility as well as immunomodulatory,angiogenic,and osteogenic capabilities;they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration,tube formation,and angiogenic differentiation of human umbilical vein endothelial cells(HUVECs)and facilitated the migration,osteodifferentiation,and extracellular matrix(ECM)mineralization of MC3T3-E1 *** in vivo evaluations showed that these piezoelectric hydrogels with versatile capabilities significantly facilitated new bone formation in a rat large-sized cranial injury *** underlying molecular mechanism can be partly attributed to the immunomodulation of the Cs/Gel/PHA/PBT hydrogels as shown via transcriptome sequencing analysis,and the PI3K/Akt signaling axis plays an important role in regulating macrophage M2 ***:The piezoelectric Cs/Gel/PHA/PBT hy

关键词： Piezoelectric hydrogel Tissue engineering scafold Immunomodulation Angiogenesis Osteogenic differentiation

面向环境不确定性的船舶操纵运动建模与预报方法

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交通运输工程学报 2024年第3期24卷 279-295页

作者：陈立家周欣蔚杨沛艺王凯李胜为武汉理工大学航运学院湖北武汉430063 武汉理工大学内河航运技术湖北省重点实验室湖北武汉430063 武汉理工大学水路交通控制全国重点实验室湖北武汉430063

为解决复杂环境因素影响下的船舶操纵运动预报精度问题,提出了一种面向环境不确定性的船舶操纵运动灰箱辨识建模与预报方法;参考分离型船舶操纵运动模型结构,考虑船舶操纵运动机理,建立了简化的灰箱模型;选取合适的试验对象,运用最小二... 详细信息

为解决复杂环境因素影响下的船舶操纵运动预报精度问题,提出了一种面向环境不确定性的船舶操纵运动灰箱辨识建模与预报方法;参考分离型船舶操纵运动模型结构,考虑船舶操纵运动机理,建立了简化的灰箱模型;选取合适的试验对象,运用最小二乘支持向量机算法对建立的船舶操纵运动灰箱模型进行参数辨识,并通过旋回试验和Z形操纵试验测试了模型的泛化性;通过环境不确定性因素分析,构建了波浪作用力干扰模型、数据传输延时模型和感知设备误差模型,并以此为基础,生成了具有多种环境不确定性因素影响的船舶运动响应训练数据;通过仿真试验,验证了预报方法在环境不确定性因素干扰下的预报精度。研究结果表明:在引入环境不确定性因素影响的船舶操纵运动预报试验中,当感知设备误差由0逐渐提升至5%和10%时,除受较小的初始数量级因素影响的横摇速度外,其余船舶运动响应预报结果的均方根误差增幅均小于10%,预测模型的精度可以得到有效保证;而在感知设备误差达到20%的极端条件下,纵荡速度、横荡速度、艏摇速度预报误差相较于0时分别提升4.65%、15.97%、18.17%,误差增幅仍能有效控制在20%以下。可见,船舶操纵运动建模与预报方法可在一定程度上实现环境不确定性因素干扰下的高精度船舶操纵运动预报。

关键词：水路运输船舶操纵运动环境不确定性最小二乘支持向量机建模与预报方法验证试验