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Assessment of wheat variety distinctness using SSR markers

Journal of Integrative Agriculture 2015年第10期14卷 1923-1935页

作者： wang li-xin QIU Jun CHANG li-fang liU li-hua li Hong-bo PANG Bin-shuang ZHAO Chang-ping Beijing Engineering and Technique Research Center for Hybrid Wheat Beijing Academy of Agricultural and Forestry Sciences National Agriculture Technology Extension and Service Center Ministry of Agriculture National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science Chinese Academy of Agricultural Sciences

Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat（SSR） markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value（GSV） ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.

关键词： wheat variety distinctness assessment SSR markers

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Syntheses of novel series benzo [1,5] oxazepin-4-one-based compounds from 1,5-difluoro-2,4-dinitrobenzene

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Chinese Chemical Letters 2008年第9期19卷 1021-1023页

作者： li Qiu wang Qing Shan li xin Pu Wei Wei Nie Xiao Fei Lv Qiu Rong li Yanshan University Qinhuangdao 066004 China

A novel benzo [ 1,5] oxazepin-4-one skeleton compound and its four derivatives were synthesized effectively from 1,5-difluoro- 2,4-dinitrobenzene （DFDNB） under mild conditions. In the process, four intermediates were synthesized by substitutions of the two fluorine atoms and reductions of the meta-dinitro groups in DFDNB respectively. The results showed that the key for synthesizing the intermediates was the substitution of one of the two fluorine atoms in DFDNB by 3-hydroxy butyric acid ethyl ester first, then the other fluorine atom by morpholine, and then the reduction of the meta-dinitro groups in the substitute by HCOONH4 with Pd/C. The products were purified with silica gel column chromatography, and confirmed by HPLC, LC-MS and 1H NMR. They should contribute to construct the molecular libraries for therapeutic applications.

关键词： Syntheses Benzo oxazepin derivatives DFDNB Substitution Reduction

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Failure Analysis of Dissimilar Metal Welds between Ferritic Heat Resistant Steels and Austenitic Stainless Steels in Power Plant

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Chinese Journal of Mechanical Engineering 2024年第4期37卷 306-326页

作者： Xiaogang li Junfeng Nie xin wang Kejian li Haiquan Zhang Institute of Nuclear and New Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of EducationTsinghua UniversityBeijing 100084China Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China

This study analysed the failure of dissimilar metal welds(DMWs)between ferritic heat resistant steels and austenitic stainless steels and investigated its influencing factors by means of numerical simulation,microstructure characterization and mechanical property *** the long-term high-temperature service condition in practical power plant,the DMW failure mode was along the interface between nickel-based weld metal(WM)and ferritic heat resistant steel,and the failure mechanism was stress/strain concentration,microstructure degradation and oxidation coupling acting on the *** numerical simulation results show that interface stress/strain concentration was due to the differences in coefficient of thermal expansion and creep strength,and the degree of stress/strain concentration was related to service *** ferrite band formed at the WM/ferritic steel interface was prone to cracking,attracting the fracture along the *** interface crack allowed oxidation to develop along the WM/ferritic steel *** long-term service,the interface stress/strain concentration,microstructure and oxidation all evolved,which synergistically promoted interface failure of ***,only under the long-term service of low stress conditions could trigger the interface failure of ***,long-term service would reduce the mechanical strength and plasticity of DMW.

关键词： Dissimilar metal weld Failure Interface Stress/strain concentration Microstructure Oxidation

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Knockout of C6orf120 in Rats Alleviates Concanavalin A-induced Autoimmune Hepatitis by Regulating Macrophage Polarization

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Biomedical and Environmental Sciences 2024年第6期37卷 594-606页

作者： xin wang Yuqi wang Hui liu Yingying lin Peng wang Yunyun Yi xin li Department of Center of Integrated Traditional Chinese and Western Medicine Peking University Ditan Teaching HospitalBeijing 100015China Department of Center of Integrated Traditional Chinese and Western Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Department of Center of Infectious Disease Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 *** Six–eight-week-old C6orf120^(-/-)and wild-type(WT)SD rats were injected with Con A(16 mg/kg),and euthanized after 24 *** sera,livers,and spleens were ***-1 cells and the recombinant protein(rC6ORF120)were used to explore the mechanism in *** frequency of M1 and M2 macrophages was analyzed using flow *** blotting and PCR were used to detect macrophage polarization-associated *** C6orf120 knockout attenuated Con A-induced autoimmune *** cytometry indicated that the proportion of CD68^(+)CD86^(+)M1 macrophages from the liver and spleen in the C6orf120^(-/-)rats decreased.C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α,IL-1β,and IL-6 in the liver.C6orf120 knockout did not affect the polarization of THP-1 ***,rC6ORF120 promoted the THP-1 cells toward CD68^(+)CD80^(+)M1 macrophages and inhibited the CD68^(+)CD206^(+)M2 *** C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120^(-/-)rats.

关键词： C6orf120 Autoimmune hepatitis Macrophage polarization M1 macrophages

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Threshold Selection and Resource Allocation for Quantized Identification

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Journal of Systems Science & Complexity 2024年第1期37卷 204-229页

作者： wang Ying li xin ZHAO Yanlong ZHANG Ji-Feng Key Laboratory of Systems and Control Institute of Systems ScienceAcademy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100049China

This paper is concerned with the optimal threshold selection and resource allocation problems of quantized identification, whose aims are improving identification efficiency under limited resources. Firstly, the first-order asymptotically optimal quantized identification theory is extended to the weak persistent excitation condition. Secondly, the characteristics of time and space complexities are established based on the Cramér-Rao lower bound of quantized systems. On these basis, the optimal selection methods of fixed thresholds and adaptive thresholds are established under aperiodic signals, which answer how to achieve the best efficiency of quantized identification under the same time and space complexity. In addition, based on the principle of maximizing the identification efficiency under a given resource, the optimal resource allocation methods of quantized identification are given for the cases of fixed thresholds and adaptive thresholds, respectively, which show how to balance time and space complexity to realize the best identification efficiency of quantized identification.

关键词： Quantized output resource allocation system identification threshold selection.

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Rapid prediction of damaged ship roll motion responses in beam waves based on stacking algorithm

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Journal of Hydrodynamics 2024年第2期36卷 394-405页

作者： xin-ran liu Ting-qiu li Zi-ping wang School of Naval Architecture Ocean and Energy Power EngineeringWuhan University of TechnologyWuhan430063China

Accurate modeling for highly non-linear coupling of a damaged ship with liquid sloshing in waves is still of considerable interest within the computational fluid dynamics(CFD)and AI *** paper describes a data-driven Stacking algorithm for fast prediction of roll motion response amplitudes in beam waves by constructing a hydrodynamics model of a damaged ship based on the dynamic overlapping grid CFD *** general idea is to optimize various parameters varying with four types of classical base models like multi-layer perception,support vector regression,random forest,and hist gradient boosting *** offers several attractive properties in terms of accuracy and efficiency by choosing the standard DTMB 5415 model with double damaged compartments for *** is clearly demonstrated that the predicted response amplitude operator(RAO)in the regular beam waves agrees well with the experimental data available,which verifies the accuracy of the established damaged ship hydrodynamics *** high-quality CFD samples,therefore,implementation of the designed Stacking algorithm with its optimal combination can predict the damaged ship roll motion amplitudes effectively and accurately(e.g.,the coefficient of determination 0.9926,the average absolute error 0.0955 and CPU 3s),by comparison of four types of typical base models and their various ***,the established Stacking algorithm provides one potential that can break through problems involving the time-consuming and low efficiency for large-scale lengthy CFD simulations.

关键词： Ship motion damaged ship computational fluid dynamics(CFD) machine learning stacking algorithm

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Experimental validation of adsorption filter model under dynamic VOC concentrations:Prediction of long-term efficiency

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Building Simulation 2024年第7期17卷 1201-1212页

作者： Ruiyan Zhang Ziying li Xiangyuan Guan xin wang Fei wang lingjie Zeng Zhenhai li School of Environment and Architecture University of Shanghai for Science and TechnologyShanghai 200093China School of Mechanical Engineering Tongji UniversityShanghai 200092China

Indoor volatile organic compound(VOC)concentrations are often dynamic because the ventilation and emission rates of VOC usually *** filters used for air purification may operate with a capacity that fluctuates with unsteady VOC concentrations in *** the dynamic interactions between adsorption filters and indoor air is crucial for predicting their performance under real-world *** study presents a numerical model of partially reversible adsorption equilibrium coupled with a mass transfer model to create a predictive model for adsorption efficiency in environments with dynamic VOC concentrations.A honeycomb adsorption filter for benzene adsorption was simulated and tested,including the breakthrough and purging curve and the long-term efficiency in an experimental chamber with dynamic *** results reveal that the curve generated with the partially reversible adsorption equilibrium model closely aligns with the measured ***,the model was coupled with a chamber model and the simulation results were compared with those calculated using the filter model with a single adsorption *** VOCs were emitted intermittently in the chamber and there was sufficient ventilation,the concentration peaks in the chamber derived from the models with different assumptions on adsorption reversibility were significantly different from each ***,it was observed that the reversible adsorption capacity of the filter was crucial for long-term operation in rooms with dynamic *** the reversible adsorption capacity constituting only 6.7%of the total adsorption capacity of the tested filter,it contributes to a significant“peak shaving and valley filling”effect,even when the irreversible adsorption capacity is *** adsorption reversibility should be taken as an important parameter for selecting adsorbents for dynamic concentration conditions.

关键词： adsorption filter efficiency model indoor air partially reversible adsorption

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A Distributed Adaptive Second-Order Latent Factor Analysis Model

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IEEE/CAA Journal of Automatica Sinica 2024年第11期11卷 2343-2345页

作者： Jialiang wang Weiling li xin Luo the School of Computer Science and Technology Dongguan University of Technology the College of Computer and Information Science Southwest University

Dear Editor,This letter presents a distributed adaptive second-order latent factor(DAS) model for addressing the issue of high-dimensional and incomplete data representation. Compared with first-order optimizers, a second-order optimizer has stronger ability in approaching a better solution when dealing with the non-convex optimization problems, thus obtaining better performance in extracting the latent factors(LFs) well representing the known information from high-dimensional and incomplete data.

关键词： Particle swarm optimization (PSO)

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Traction force with extracellular matrix mediated by cytoskeleton influences metastasis through SLC8A1 induced Wnt-b-catenin pathway in endometrial cancer

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Genes & Diseases 2024年第5期11卷 98-101页

作者： xingchen li xin Xu Jianliu wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Department of Obstetrics and Gynecology Beijing ChaoYang HospitalCapital Medical UniversityBeijing 100020China

Endometrial cancer(EC)is one of the most prominent malignancies in ***,despite these advancements in understanding EC pathogenesis,mortality rates have not *** force(TF)refers to endogenous mechanical forces exerted by cells.1 These forces act on the extracellular matrix and the extracellular tumor microenvironment through focal adhesions,thereby regulating cell proliferation and ***,the TF characteristics of endometrial cancer cells and their heterogeneity remain unclear.

关键词： endometrial metastasis endogenous

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强化矿区周边重金属铅污染土壤电动修复技术

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广州化工 2024年第4期52卷 126-128,148页

作者：王丽鑫蔡宗平李炜键胡嘉沛李思缘李曼婷广东环境保护工程职业学院广东省环境保护矿冶行业重金属污染防治与职业教育重点实验室广东省固体废弃物资源化与重金属污染控制工程技术研究中心广东佛山528216 广州天海环保科技有限公司广东广州510655

通过对铅锌矿山周边铅污染土壤进行电动修复8天,重金属铅总浓度去除率约36.7%,采用间隔断电强化电动修复的方法进行修复,重金属铅的去除率达55.7%,高于传统电动修复。对于土壤有效态铅,间隔断电电动修复法去除率为66.9%,同样高于传统电... 详细信息

通过对铅锌矿山周边铅污染土壤进行电动修复8天,重金属铅总浓度去除率约36.7%,采用间隔断电强化电动修复的方法进行修复,重金属铅的去除率达55.7%,高于传统电动修复。对于土壤有效态铅,间隔断电电动修复法去除率为66.9%,同样高于传统电动修复的有效态铅52.2%去除率。实验证明,间隔断电法在断电又通电的一段时间内提高土壤中的电流强度,使得电流的下降段变长,有利于提高去除率,是一种有效的强化电动修复土壤重金属的方法。

关键词：电动修复土壤重金属污染间隔通电强化

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