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Enhanced N2O emission rate in field soil undergoing conventional intensive fertilization is attributed to the shifts of denitrifying guilds

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Pedosphere 2021年第1期31卷 145-156页

作者： Mengmeng JI Hao TIAN Xiaoging WU Ji LI Ying ZHU Guojun WU Ting XU jingguo wang Xiaojun ZHANG Siate Key Laberaory of Micnbrial Metabolisom and School of Life Scriences and Bioechmology.Shanghai Jiav Tong CUmiesiy Shonghal 200240China College of Resoures and Envirommental Sciences China Agricwlural CniversilyBeijing 100193China

The emission of greenhouse gas N2O in agricultural soil is modulated by N fertilization that could be converted to N2O by denitrifiers under anaerobic condition. Nevertheless, the effect of denitrifiers on N2O emission has not been thoroughly elucidated. In this study, we explored the denitrifying gas kinetics,nitrate content, transcribed denitrifying functional genes(narG, nirS, nirK, and nosZ), and the active bacteria during anaerobic incubation of soils with conventional intensive N fertilization(CNS) and reduced N fertilization(RNS), both sampled from a vegetable greenhouse experimental site. The CNS sample showed significantly higher N2O emission rates relative to the RNS sample. However, the difference in N2O emission between the soils was neither because of the cumulative nitrate content nor the quantity of denitrifying gene transcripts. The distinct fertilization regimes shaped the significantly different bacterial communities in these soils. The absolute abundance of bacteria that produce N2O but lack the nosZ gene for N2O reduction(for example, the dominant Kaistobacter) was higher in CNS than in RNS. Meanwhile, the abundance of two operational taxonomy units(OTUs), namely Rhodanobacter,belonging to the most abundant genus in denitrifying guilds, was strongly enriched in CNS and showed significant positive correlation with N2O/(N2O +N2). The predominance of these bacterial OTUs in the CNS denitrifying guild strongly suggested that high N2O emission from the soil with long-term conventional intensive fertilization might be primarily attributed to the reshaping of distinct denitrifying guilds in their bacterial communities.

关键词： denitrification denitrifier community denitrifying bacteria denitrifying functional gene greenhouse gas N fertilization

Phase Relations in TiAl+Nb System

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Journal of Materials Science & Technology 1994年第5期10卷 359-366页

作者： jingguo wang Guoliang CHEN and Zuqing SUN (State Key Lab. for Advanced Metal Materials, University of Science and Technology Beijing, Beijing, 100083, China)Hengqiang YE (Lab. of Atomic Imaging of Solids, Institute of Metal Research, Academia Sinica, Sh Univ of Science and Technology Beijing China

The microstructures and phases of ternary TiAl+Nb alloys containing 50-60 at.-%Al, 0-21 at.-%Nb have been studied using transmission electron microscopy (TEM) and X-ray powder diffraction. The phases present in the alloys and their distribution were found to be a sensitive function of composition. The phase relations between γ-TiAl and γ1 (a new ordered ternary intermetallic compound based on γ-TiAl) were determined. Essentially single γ phase was determined for alloys with relativety low Nb content (≤10 at.-%Nb). the γ1 phase was determined to exist in the composition range containing higher Nb contents (15-21 at.-%Nb). Between γ and γ1 phases, with intermediate Nb contents there is a transitional phase γ1 (a superstructure of γ-TiAl). As for the influence of Al concentration on the phase relations. the γ1 phase was inclined to form in the alloys with relatively high Al contents. The ordering transformation of γ, to γ1 is a continuous ordering process and the transition may be second order.

关键词： TiAl nm PA Phase Relations in TiAl+Nb System Chen

Risk assessment study on bridge foundation in deep overlying stratum

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Engineering Sciences 2012年第3期10卷 38-43页

作者： Zhang Xiangqun wang jingguo Liu Manhong Jiangsu Provincial Yangtze River Highway Bridge Construction Commanding Department Taizhou Jiangsu 225321 China College of Geological Sciences and Engineering Hohai University Nanjing 210098 China

Based on the analysis about the hydrogeological conditions and engineering geological conditions, this paper makes analysis on the possible risks of the deep overlying stratum foundation and establishes the risk evaluation index system during the foundation operating period. Such methods as analytic hierarchy process （AHP） , Delphi method and fuzzy comprehensive evaluation method are adopted to make the quantitative analysis on the risk factors and establish the risk judgment model. According to the actual engineering of Taizhou Bridge, the paper evaluates the risk of the foundation during the operating period at the condition of deep overlying stratum. The evaluation results can provide the reference for the risk management of the bridge foundation durin~ the ooerating period.

关键词： risk assessment deep overlying stratum suspension bridge AHP

Elite sd1 alleles in japonica rice and their breeding applications in northeast China

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The Crop Journal 2022年第1期10卷 224-233页

作者： Hanjing Sha Hualong Liu Guangxin Zhao Zhongmin Han Huilin Chang jingguo wang Hongliang Zheng Jifeng Zhang Yang Yu Yuqiang Liu Detang Zou Shoujun Nie Jun Fang Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina Innovation Center Suihua Branch of Heilongjiang Academy of Agricultural SciencesSuihua 152052HeilongjiangChina University of Chinese Academy of Sciences Beijing 100049China College of Life Science and Engineering Shenyang UniversityShenyang 110044LiaoningChina The Innovative Academy for Seed Design Chinese Academy of SciencesBeijing 100101China

The Green Revolution gene sd1 has been used extensively in modern rice breeding,especially in indica ***,elite sd1 alleles and related germplasm resources used for japonica rice breeding have not been identified,and extensive efforts are needed for japonica rice breeding to obtain new dwarfing *** from MBKbase-Rice revealed seven sd1 haplotypes in indica and four in japonica *** new sd1 alleles were identified in indica *** 295 japonica accessions from northeast Asia,except for the weak functional allele SD1-EQ,sd1-r was the major allele,reducing plant height in comparison with *** germplasm resources carrying reported sd1 alleles were identified by genotype searching and further verified by literature search,genealogical analysis,and d Caps *** and geographic distribution showed that sd1-r is an excellent allele widely used in northern China and Tohoku in Japan,and sd1-j is commonly used in east China and Kyushu in ***-and Xiushui-series cultivars carrying sd1-r and sd1-j,respectively,are essential branches of the backbone parents of Chinese japonica rice,Akihikari and Ce21,with the largest number of descendants and derived *** semi-dwarf japonica rice breeding,sd1-d was introgressed into Daohuaxiang 2(DHX2).Dwarf and semi-dwarf lines carrying sd1-d were selected and designated as 1279 and 1280,respectively,after withstanding typhoon-induced strong winds and heavy rains in 2020,and are anticipated to become useful intermediate materials for future genetic research and *** work will facilitate the introduction,parental selection,and marker-assisted breeding,and provide a material basis for the next step in identifying favorable genes that selected together with the sd1 alleles in japonica backbone parents.

关键词： Semi-dwarf breeding Elite allele Pedigree analysis Geographic distribution Regional adaptability

Study on Difference in Cold Tolerance Between Weedy Rice and Cultivated Rice in Heilongjiang Province of China

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Journal of Northeast Agricultural University(English Edition) 2008年第1期15卷 1-7页

作者： ZOU Detang GUO Junxiang LIU Hualong wang jingguo College of Agriculture Northeast Agricultural University Harbin 150030 China

A long-red awn weedy rice and rice cultivars named Ken99004（ZCI）, Crossing-503（ZC2）, Ken Sticky Rice（ZC3）, Shashani（ZC4）, Long-Grain Aromatic Rice（ZC5） were used to study typical wild traits of weedy rice and to identify the cold resistance of all genotypes. The results showed that the stem of long-red awn weedy rice was the weakest. No difference was found in the early stage of rice growth, e.g. the vegetable growth stage, between long-red awn weedy rice and other rice cultivars in the stem rigidity, but in the later stage, or reproductive growth stage, their leaves and stems were senescent rapidly because of the speedy supply of nutrients for panicle growth, meanwhile the rigidity of stem was reduced sharply just as withered weeds. The germination rate of long- red awn weedy rice was the highest in cold condition, and in turn were ZCI, ZC2, ZC3, ZC4, ZC5, respectively. The performances of genotypes in cold tolerance were identical, those with a high ability of germination in low temperature also showed a strong cold tolerance in main traits in whole growth period, the order from strong to weak in the extent of cold tolerance were long-red awn weedy rice, ZCl, ZC2, ZC3, ZC4, ZC5, respectively

关键词： weedy rice genotype cold tolerance

OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice(Oryza sativa L.)

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The Crop Journal 2021年第5期9卷 1003-1017页

作者： Jiaming Li Minghui Zhang Luomiao Yang Xinrui Mao Jinjie Li Lu Li jingguo wang Hualong Liu Hongliang Zheng Zichao Li Hongwei Zhao Xianwei Li Lei Lei Jian Sun Detang Zou Key Laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina College of Life Science Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement China Agricultural UniversityBeijing 100094China

The C (Cys) 2H (His) 2-type transcription factor is one of the most important transcription factors in plants and plays a regulatory role in the physiological responses of rice to abiotic stresses.A novel rice C2H2-type zinc finger protein,abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3),was found to confer drought stress tolerance by enhancing antioxidant defense and regulating Os *** of OsADR3 in rice increased tolerance to drought stress by increasing ROS scavenging ability and ABA *** contrast,CRISPR/Cas9-mediated knockout of osadr3 increased the sensitivity of rice to drought and oxidative *** exogenous ROS-scavenging reagent restored the droughtstress tolerance of osadr3-CRISPR *** transcriptome analysis suggested that OsADR3 increased the expression of Os GPX1 under drought *** mobility shift,yeast one-hybrid,and dualluciferase reporter assays revealed that OsADR3 modified the expression of Os GPX1 by directly binding to its *** of Os GPX1 repressed ROS scavenging ability under drought stress in OsADR3-overexpression *** findings suggest that OsADR3 plays a positive regulatory role in droughtstress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice.

关键词： Drought stress Rice ROS scavenging Glutathione peroxidase Abscisic acid

Identification of Cold Tolerance of Rice Germplasm Resource at Germinating Stage in Cold Region

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Journal of Northeast Agricultural University(English Edition) 2008年第2期15卷 1-4页

作者： LIU Hualong SUN Shiche wang jingguo ZOU Detang College of Agriclture Northeast Agricultural University Harbin 150030 China Heilongjiang Academy of Agricultural Sciences Harbin 150086 China

Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance. The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant （HT）, tolerant （T）, moderately tolerant （MT）, susceptible （S）, highly susceptible （HS）.

关键词： rice cold tolerance germplasm resource identification

Isolation and identification of allelochemicals in the decomposition products from soybean residues

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Isolation and identification of allelochemicals in the decom...

电荷转移络合分光光度法测定硫酸阿托品注射液含量

中国第二届植物化感作用学术研讨会暨中国植物保护学会植物化感作用专业委员会成立大会

作者： YAN Fei wang jingguo YANG Zhenming SONG Fengbin College of Plant Science Jilin University Northeast Institute of Geography and Agricultural Ecology CAS MOA Key Laboratory of Plant Nutrition MOE Key Laboratory of Soil-plant InteractionCollege of Agricultural Resources and Environmental SciencesChina Agricultural University

A crude aqueous extract from decayed soybean residues, which had been inoculated with soil suspension and incubated for 30 days, was obtained with distilled water. We extracted the crude aqueous extract by ethyl acetate under different pH values of 2, 7 and 12. No significant allelopathic effect on radicle elongation was observed for the organic phase extracted under basic or neutral media. The final aqueous phase displayed some extent of inhibitory effect. After comparing with standard sodium chloride solution at different concentrations, we found that it was the sodium chloride introduced during the extraction processes that contributed to this effect. Therefore, we are certain that the organic extract from acidic condition contained the major components that led to the allelopathic effects. The organic extract under pH of 2 was further eluted sequentially by eight solvents from petroleum ether to methanol with increasing polarity in a silica gel column (20mm x 100mm, vacuum). Eight fractions were collected and tested with the same bioassay. Our results showed that two of the fractions had the most significant inhibitory effects. Another silica gel column (15mm x 250mm) was then applied to isolate the mixture of the above two fractions with a solvent mixture of methanol and ethyl acetate as an eluant. The eluates were collected every 50ml. Thin-layer chromatography was involved to select similar eluates. These similar eluates were combined into three samples. After assayed with radicle elongation of soybean seedling, the second sample was determined to be the most toxic. The analysis with gas chromatography-mass spectrometry (GC-MS) indicated twelve compounds were present in the second sample. We identified these twelve compounds as Phenol 2, 4-bis (1,1- dimethylethyl), 1-Hexadecene, 1-Octadecene, 1-Octadecene, 9-Hexadecenoic acid, methyl ester, 8-Octadecenoic acid, methyl ester, 1-Docosene, Cyclotetracosane, Octadecanoic acid, methyl ester, Octadecanoic acid, but

关键词： Soybean (Glycine max) the decomposition products from residues allelopathic compounds isolation bioassay

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中国医院药学杂志 1999年第3期19卷 155-156页

作者：王敬国中国人民解放军第210医院

目的：测定硫酸阿托品注射液含量。方法：以２３７ｎｍ为测定波长，采用电荷转移络合分光光度法。结果：平均回收率为９９．６％，相对标准偏差为０．４％。结论：该方法快速、简便，对于含微量阿托品制剂尤为灵敏。

关键词：硫酸阿托品碘紫外分光光度法注射液