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Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS

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Food Science and Human Wellness 2024年第4期13卷 2187-2196页

作者： Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge guiying wang Livestock Product Processing and Engineering Technology Research Center of Yunnan Province Yunnan Agricultural UniversityKunming 650201China College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China

The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years(the first,second and third year sample)were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography(GC-TOF-MS)to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing *** results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes,the dominant fungi phyla were Ascomycota and Basidiomycota,while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham,***,252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years,and 12 different metabolites were screened through multivariate statistical *** metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation,of which 8 metabolic pathways had significant effects on ham fermentation(Impact>0.01,P<0.05).The content of L-proline,phenyllactic acid,L-lysine,carnosine,taurine,D-proline,betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia,but negatively correlated with the relative abundance of Halomonas,Aspergillus and Yamadazyma.

关键词： Nuodeng ham Microbial diversity Small molecule metabolites High-throughput sequencing Gas chromatography-time of flight mass spectrography

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RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2

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Science China(Life Sciences) 2024年第10期67卷 2186-2197页

作者： Lili Zhi Chaoqun Chen Ge Zhang Tian Huang Wenxia He Jinrui Zhang Dan Chen Jiayi Liu Jinyao Zhao Yangfan Qi guiying wang Wenjing Zhang Yang wang Sino-US Research Center for Cancer Translational Medicine of the Second Affiliated Hospital of Dalian Medical University&Institute of Cancer Stem Cell Dalian Medical UniversityDalian 116023China Department of Immunology College of Basic Medical SciencesDalian Medical UniversityDalian 116044China Institute of Cancer Stem Cell Dalian Medical UniversityDalian 116044China Department of Pathology the First Affiliated Hospital of Dalian Medical UniversityDalian Medical UniversityDalian 116011China Department of General Surgery the Second Hospital of Hebei Medical UniversityShijiazhuang 050000China The Second Department of General Surgery the Fourth Hospital of Hebei Medical UniversityShijiazhuang 050011China Hebei Key Laboratory of Etiology Tracing and Individualized Diagnosis and Treatment for Digestive System Carcinoma Shijiazhuang 050001China

Increasing evidence suggests that deregulated RNA splicing factors play critical roles in tumorigenesis;however,their specific involvement in colon cancer remains largely *** we report that the splicing factor RBM25 is overexpressed in colon cancer,and this increased expression correlates with a poor prognosis of patients with colon ***,RBM25 ablation suppresses the growth of colon cancer cells both in vitro and in ***,our transcriptome-wide analysis of splicing events revealed that RBM25 regulates a large number of cancer-related alternative splicing events across the human genome in colon ***,RBM25 regulates the splicing of MNK2 by interacting with the poly G rich region in exon 14a,thereby inhibiting the selection of the proximal 3'splice site(ss),resulting in the production of the oncogenic short isoform,*** of RBM25 leads to an increase in the MNK2a isoform and a decrease in the MNK2b ***,re-expression of MNK2b or blocking the 3′ss of the alternative exon 14a with ASO partially reverses the RBM25 knockdown mediated tumor ***,MNK2b levels were significantly increased in colon cancer tissues,which is positively correlated with the expression level of ***,our findings uncover the critical role of RBM25 as a key splicing factor in colon cancer,suggesting its potential as a prognostic marker and therapeutic target.

关键词： RBM25 colon cancer alternative splicing MNK2

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谈催化氧化脱硫技术在处理乙烯碱渣中的应用

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石油化工安全环保技术 2024年第2期40卷 61-64,I0004页

作者：王桂英中石化股份有限公司天津分公司烯烃部天津300270

乙烯装置在生产过程中产生的废碱液中硫化钠浓度≤1%,送高含盐水装置去除硫化钠后,再进入污水处理场处理合格后外排。2021年由于原料变化,废碱液中硫化物浓度增加到达2%~3%,超过高含盐水装置的处理能力,对高含盐水装置的运行造成不利影... 详细信息

乙烯装置在生产过程中产生的废碱液中硫化钠浓度≤1%,送高含盐水装置去除硫化钠后,再进入污水处理场处理合格后外排。2021年由于原料变化,废碱液中硫化物浓度增加到达2%~3%,超过高含盐水装置的处理能力,对高含盐水装置的运行造成不利影响。为解决乙烯废碱液中硫化物浓度高的问题,2022年利用武汉俊恒科技有限公司的低温低压催化氧化脱硫技术对乙烯废碱液中硫化物进行了预处理,硫化物浓度由10000~30000mg/L降低至200mg/L以下,确保了高含盐水装置的正常运行。

关键词：乙烯废碱液低温低压催化脱硫

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先于子痫前期的严重胎儿生长受限的临床特点及围产结局

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中华围产医学杂志 2024年第9期27卷 722-728页

作者：徐霞许艳红祖逸峥王贵英颜建英福建医科大学妇儿临床医学院(福建省妇幼保健院、国家产科临床重点专科建设单位)妇产科福州350001

目的探讨以严重胎儿生长受限(fetal growth restriction,FGR)为先发症状的子痫前期(preeclampsia,PE)的临床特点及围产结局。方法本研究为回顾性队列研究,研究对象为2012年1月至2022年12月在福建省妇幼保健院分娩的PE合并严重FGR的单胎... 详细信息

目的探讨以严重胎儿生长受限(fetal growth restriction,FGR)为先发症状的子痫前期(preeclampsia,PE)的临床特点及围产结局。方法本研究为回顾性队列研究,研究对象为2012年1月至2022年12月在福建省妇幼保健院分娩的PE合并严重FGR的单胎活产病例。根据严重FGR和高血压发生的先后顺序将研究对象分为严重FGR先发组和高血压先发组。分析2组的一般资料、发病特征、妊娠并发症及新生儿结局。采用t检验、Mann-Whitney U检验和χ^(2)检验进行统计学分析。采用多因素线性回归或logistic回归分析校正混杂因素对围产结局的影响。结果(1)一般资料:共307例进入本研究,包括严重FGR先发组194例(63.2%)和高血压先发组113例(36.8%)。与高血压先发组比较,严重FGR先发组32周前严重FGR占比较高,PE诊断孕周较晚,早发型PE占比较低,终止妊娠孕周较大,诊断PE到终止妊娠间隔时间短[40.7%(46/113)与59.3%(115/194),χ^(2)=9.87;(32.8±5.1)周与(35.6±3.4)周,t=5.12;52.2%(59/113)与25.8%(50/194),χ^(2)=21.80;(34.7±3.1)周与(36.0±3.2)周,t=3.43;P值均<0.01]。2组诊断严重FGR与高血压的孕周间隔差异无统计学意义。(2)妊娠并发症:与高血压先发组比较,严重FGR先发组早产率更低,胎膜早破发生率更高[69.0%(78/113)与46.9%(91/194),χ^(2)=14.12;9.7%(11/113)与19.1%(37/194),χ^(2)=4.72;P值均<0.05]。采用多因素logistic回归分析校正终止妊娠孕周的差异后,2组妊娠并发症发生情况差异均无统计学意义。(3)新生儿结局:与高血压先发组比较,严重FGR先发组新生儿出生体重更重[(1757±605)g与(2067±684)g,t=4.12]、身长更长[(41.7±4.3)cm与(43.4±4.6)cm,t=3.10]、胎盘更重[(399±158)g与(486±147)g,t=2.36],剖宫产出生比例、重度SGA和低出生体重儿发生率更低[85.8%(97/113)与68.6%(133/194),χ^(2)=11.35;65.5%(74/113)与49.5%(96/194),χ^(2)=7.40;87.6%(99/113)与69.6%(135/194),χ^(2)=12.80;P值均<0.05],2组新生儿1 min Apgar评分及NICU入住率差异无统计学意义。多因素回归分析校正终止妊娠孕周的差异后发现,与高血压先发组相比,严重FGR先发组的新生儿体重较重,剖宫产风险较低[OR(95%CI)分别为80.18(0.95~159.42)和0.51(0.26~0.99),P值均<0.05]。结论严重FGR先发的PE孕妇较高血压先发孕妇PE发病晚,围产结局相对较好。需加强对严重FGR先发的PE孕妇的血压波动水平和高血压先发的PE孕妇的胎儿生长情况监测。

关键词：子痫前期胎儿生长受限临床特点围产结局

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盐津乌骨鸡与瓢鸡屠宰性能与肉品质的比较

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食品工业科技 2024年第12期45卷 241-247页

作者：杨源荀文廖国周杨春芳吴红艳葛长荣王桂瑛云南农业大学食品科学技术学院云南昆明650201 云南农业大学云南省畜产品加工工程技术研究中心云南昆明650201

为探讨盐津乌骨鸡与瓢鸡屠宰性能及肉质理化特性差异,以300日龄盐津乌骨鸡与瓢鸡为对象,对两个品种间的屠宰性能、感官与常规理化指标进行测定与比较分析。结果表明:盐津乌骨鸡与瓢鸡均具有良好肉用性能,雌性盐津乌骨鸡的胸肌率显著高... 详细信息

为探讨盐津乌骨鸡与瓢鸡屠宰性能及肉质理化特性差异,以300日龄盐津乌骨鸡与瓢鸡为对象,对两个品种间的屠宰性能、感官与常规理化指标进行测定与比较分析。结果表明:盐津乌骨鸡与瓢鸡均具有良好肉用性能,雌性盐津乌骨鸡的胸肌率显著高于雄性盐津乌骨鸡和瓢鸡(P<0.05);同一品种雄鸡的宰前体重、屠体重、全净膛重、胸肌重、腿肌重和腿肌率高于雌鸡(P<0.05),屠宰性能更好。两个品种鸡肉的外观评分均较高(6.30~7.30分),腿肉较胸肉有更高的香味、质地、滋味和总体接受性评分。两个品种鸡宰后pH均在优质鸡范围内,瓢鸡腿肉的蒸煮损失率、水分含量、粗灰分、粗脂肪均显著高于盐津乌骨鸡(P<0.05)。同一品种不同部位组间,腿肉的蒸煮损失(P<0.05)和粗蛋白含量(P<0.05)低于胸肉;而水分(P<0.05)和粗脂肪(P<0.05)含量高于胸肉。本研究为两种优良地方鸡种的深度开发利用提供科学理论依据,也为云南优质地方鸡品质评价标准的建立奠定基础。

关键词：盐津乌骨鸡瓢鸡屠宰性能肉品质

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基于环境DNA评价鸭绿江口底栖生态质量状况

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海洋学报 2024年第3期46卷 75-88页

作者：王桂营李宏俊邵魁双刘滨玮楚奇叶金清生态环境部国家海洋环境监测中心辽宁大连116023

为研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,本研究采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学进行鉴定,并对所得生态质量评价指数(AMBI、BENTIX、香浓-维纳H'、M-AMBI)进行比较分析。结果显示:环境DNA鉴定生物... 详细信息

为研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,本研究采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学进行鉴定,并对所得生态质量评价指数(AMBI、BENTIX、香浓-维纳H'、M-AMBI)进行比较分析。结果显示:环境DNA鉴定生物隶属于10纲16目19科20属22种,形态学鉴定生物隶属于9纲27目43科55属57种,共有生物10种;两种鉴定方法得出的AMBI指数间(R=0.428,p=0.043,y=0.32x+1.08)、BENTIX指数间(R=0.430,p=0.043,y=0.28x+3.59)存在显著一致性,而香浓-维纳H'指数间存在显著差异性;两种鉴定方法得出的AMBI等级间、M-AMBI等级间相似性较高,分别为51.02%、44.90%;两种鉴定方法得出的AMBI与M-AMBI等级更符合实际情况,且评价鸭绿江口整体生态质量状况为良。本研究表明,基于环境DNA鉴定底栖生物评价生态质量状况,在海洋环境监测调查中具有较高的应用潜力。

关键词：环境DNA 底栖生物生态质量

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Effects of stochastic food deprivation on energy budget,body mass and activity in Swiss mice

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Current Zoology 2009年第4期55卷 249-257页

作者： Zhijun ZHAO Jing CAO Ye TIAN Ruirui wang guiying wang School of Agricultural Science Liaocheng University Liaocheng Shandong 252059 China

When small animals are faced with an unpredictable food supply,they can adapt by altering different components of their energy budget such as energy intake,metabolic rate,rate of non-shivering thermogenesis(NST)or *** present study examined the effect of stochastic food deprivation(FD)on body mass,food intake,resting metabolic rate(RMR),NST and behaviour in male Swiss *** a period of 4 weeks' FD,animals were fed ad libitum for a randomly assigned 4 days each week,but were deprived of food for the other 3 *** results showed that body mass significantly dropped on FD days compared to *** intake of FD mice increased significantly on ad libitum days,ensuring cumulative food intake,final body mass,fat mass,RMR and NST did not differ significantly from ***,gastrointestinal tract mass increased in FD mice,but digestibility *** general,activity was higher on deprived days,and feeding behaviour was higher on ad libitum days suggesting that Swiss mice are able to compensate for stochastic FD primarily by increasing food intake on ad libitum days,and not by reducing energy expenditure related to RMR or NST.

关键词： Behaviour Body mass Food availability Food intake Swiss mice

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Improvement in bioconversion efficiency and reduction of ammonia emission by introduction of fruit fermentation broth in a black soldier fly larvae and kitchen waste conversion system

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Insect Science 2023年第4期30卷 975-990页

作者： Lusheng Li Lifei Chen Rongsheng Shang guiying wang Jibin Zhang School of Agricultural Science and Engineering Engineering Research Center of Shandong Province for Black Soldier Fly Breeding and Organic Waste ConversionLiaocheng UniversityLiaochengShandong ProvinceChina State Key Laboratory of Agricultural Microbiology National Engineering Research Centre of Microbial PesticidesCollege of Life Science and TechnologyCollege of Animal Science&TechnologyHuazhong Agricultural UniversityWuhanChina

The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is an insect commonly used for the bioconversion of various organic *** only can the BSF convert organic waste into macromolecular organic substances,such as insect pro-teins,but it can also lessen the pollution associated with these waste products by reducing ammonia emissions,for *** this study,we measured the effects of adding fruit fermentation broth(Fer)and commercial lactic acid bacteria fermentation broth(Em)to kitchen waste(KW),as deodorizing auxiliary substances,on the growth performance of black soldier fly larvae(BSFL),the intestinal flora structure of BSFL,the ammonia emis-sion from the KW substrate,and the microbial community structure of the KW *** found that the addition of Fer or Em increased the body weight of BSFL after 6 d of culture,increasing the growth rate by 9.96%and 7.96%,*** addition of Fer not only reduced the pH of the KW substrate but also increased the relative abundance of probiotics,such as Lactobacillus,Lysinibacillus,and Vagococcus,which inhibited the growth of ammonifiers such as Bacillus,Oligella,Paenalcaligenes,Paenibacillus,Pseu-dogracilibacillus,and Pseudomonas,resulting in the reduction of ammonia emission in the KW ***,the addition of Fer or Em significantly increased the rela-tive abundances of Bacteroides,Campylobacter,Dysgonomonas,Enterococcus,and Ig-natzschineria in the gut of BSFL and increased the species diversity and richness in the K W *** findings provide a novel way to improve the conversion rate of organic waste and reduce the environmental pollution caused by BSF.

关键词： ammonia emissions black soldier fly fruit fermentation broth growth per-formance kitchen waste lactic acid bacteria fermentation broth

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Enhancement of room-temperature magnetoresistance in La_(0.6)Dy_(0.1)Sr_(0.3)MnO_3/Ag_x

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Rare Metals 2010年第2期29卷 126-131页

作者： SONG Qixiang NIU Xiaofei wang guiying TANG Yonggang CAI Zhirang PENG Zhensheng Anhui Key Laboratory of Spintronic and Nanometric Materials (Cultivating Base) Suzhou College Suzhou 234000 China Structure Research Laboratory University of Science and Technology of China Hefei 230026 China

The samples of La0.6Dy0.1Sr0.3MnO3/（Ag2O）x/2（x = 0.00, 0.02, 0.04, 0.06, 0.08, 0.10, 0.20, 0.25, and 0.30） were prepared by using the solid-state reaction *** magnetic property, transport behavior, transport mechanism and magnetoresistance effect were studied through the measurements of magnetization-temperature（M-T） curves, ρ-T curves and the fitting of ρ-T *** results indicated that Ag could take part in the reaction when the doping amount is ***, when the doping amount is comparatively large, Ag as metallic state mainly deposits on the grain boundary of La0.6Dy0.1Sr0.3MnO3, and then the system forms a two-phase *** the Ag doping amount is 30% mole ratio, the resistivity of the sample is one order of magnitude smaller than that of low doped samples, and its peak of magnetoresistance at 292 K and in the magnetic field of 0.2 T strengthens apparently and reaches 16.3%, which is over 7 times as large as 2.2% of *** two-phase composite system of magnetoresistance based on perovskite manganite consists of two parts：intrinsic magnetoresistance and extrinsic ***, extrinsic magnetoresistance comes from spin-dependent scattering（SDS） and spin-polarized tunneling（SPT）.Magnetoresistance near TC increases due to the contribution of intrinsic magnetoresistance and extrinsic magnetoresistance formed by SDS, and magnetoresistance at low temperature is extrinsic magnetoresistance formed by SPT.

关键词： room temperature low-field magnetoresistance two-phase composite perovskite manganite

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Destruction of charge ordering phase in La_(0.4)Ca_(0.6)MnO_3 induced by low Cr doping

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Rare Metals 2011年第3期30卷 241-246页

作者： PENG Zhensheng YANG Gang wang guiying TANG Yonggang GUO Huanyin MAO Qiang Anhui Key Laboratory of Spintronic and Nanometric Materials Cultivating Base Suzhou College Suzhou 234000 China Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China Hefei 230026 China Department of Electronics and Electric Engineering Suzhou College Suzhou 234000 China

Polycrystalline samples of La0.4Cao.6Mn1-xCrxO3 （x = 0.00, 0.02, 0.04, 0.06） were prepared by the solid state reaction method. The influence of Cr3＋ substitution for Mn3＋ on the magnetic property and charge ordering phase of La0.4Ca0.6MnO3 was studied through the measurements of X-ray diffraction （XRD）, magnetization-temperature （M-T） curves and electron spin resonance （ESR） spectra. The experimental results indicate that the mother＇s body of La0.4Ca0.6MnO3 has very complicated magnetic structure, exhibits charge ordering phase at 258 K, and shows long-range strongly correlated charge ordering-antiferromagnetism （CO-AFM） phase from 175 to 50 K. Spin glass state appears when the temperature decreases to about 41 K. When the Cr substitution amount is x = 0.06, the charge ordering phase of the mother＇s body is de-stroyed, because the Cr3＋ substitution for Mn3＋ destroys the spin order of CE-type antiferromagnetism, and thus leads to the melting of charge ordering. It is verified experimentally that the strong coupling between charge order and spin order exists in the charge order system of CE-type antiferromagnetism.

关键词： perovskite manganite chromium doping charge ordering spin-glass state

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