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Nano Research 2015年第12期8卷 3815-3822页

作者： Lingxiao wang Jing Geng Wenhai wang chao yuan Long Kuai Baoyou Geng College of Chemistry and Materials Science The Key Laboratory of Functional Molecular Solids Ministry of Education Anhui Laboratoryof Molecular-Based Materials Center for Nano Science and Technology Anhui Normal University No. 1 Beijing East Road Wuhu 241000 China

The sluggish oxygen evolution reaction （OER） is an important half-reaction of the electrochemical water-splitting reaction. Amorphous Fe/Ni composite oxides have high activity. In this work, we modified the aerosol spray-assisted approach and obtained amorphous Fe-Ni-Ox solid-solution nanoparticles （Fe-Ni-Ox-NPs） approximately 20 nm in size by choosing iron/nickel acetylacetonates as raw materials instead of inorganic salts. The small-sized Fe-Ni-Ox-NPs were characterized by scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, X-ray diffraction （XRD） analysis, energy-dispersive X-ray spectroscopy （EDX）, and X-ray photoelectron spectroscopy （XPS）. Furthermore, an investigation of electrochemical OER performance suggests that the small-sized Fe-Ni-Ox-NPs have higher activity than the large-sized Fe-Ni-Ox-MPs. A small overpotential of 0.315 V was demanded to obtain a working current density of 50 mA/cm2, and the Tafel slope was as low as 38 mWdec.

关键词： Fe-Ni-Ox solid solution electrocatalysis oxygen evolution reaction

Using a Suppression Subtractive Library-Based Approach to Identify Non-Heading Chinese Cabbage Genes Up-Regulated in Early Response to Elicitor PB90

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Agricultural Sciences in China 2008年第3期7卷 303-313页

作者： GAN Yun-zhe ZHANG Zheng-guang wang yuan-chao ZHENG Xiao-bo Key Lab of Monitoring and Management of Plant Disease and Insects Ministry of Agriculture/College of Plant Protection Nanjing Agricultural University Nanjing 210095 P.R.China

Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. Transcriptional responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana; however, corresponding data aren＇t available for non-heading Chinese cabbage. To address this problem, we describe a suppression subtractive library-based approach to isolate the plant＇s ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases, 70 up-regulated genes were classified into 9 parts： some aspect of primary ＇metabolism＇ or ＇energy＇ production; ＇protein synthesis＇ or ＇protein fate＇; cellular communication/signal transduction mechanism; cell fates including Beclin, SPT1, and SPT2; HLA-B and AGO1 which participate in transcription; cellular transport and hypothetical proteins or proteins for which a function has yet to be determined. Seven selected genes such as Beclin, thioredoxin, HLA-B, MAP3K, SPT1, SPT2, and AGO1 were up-regulated induced by PB90, suggesting that the genes may play an important role in PB90-triggered HR.

关键词： PB90 non-heading Chinese cabbage suppression subtractive hybridization up-regulated hypersensitive response

A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens

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Journal of Integrative Agriculture 2020年第8期19卷 2056-2063页

作者： YE Wen-wu ZENG Dan-dan XU Miao YANG Jin MA Jia-xin wang yuan-chao ZHENG Xiao-bo Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095P.R.China Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of EducationNanjing 210095P.R.China

Soybean root diseases are associated with numerous fungal and oomycete pathogens;however,the community dynamics and interactions of these pathogens are largely *** performed 13 loop-mediated isothermal amplification(LAMP)assays that targeted specific soybean root pathogens,and traditional isolation assays.A total of 159 samples were collected from three locations in the Huang-Huai-Hai region of China at three soybean growth stages(30,60,and 90 days after planting)in *** LAMP results,we found that pathogen communities differed slightly among locations,but changed dramatically between soybean growth *** sojae,Rhizoctonia solani,and Fusarium oxysporum were most frequently detected at the early stage,whereas Phomopsis longicolla,Fusarium equiseti,and Fusarium virguliforme were most common in the later *** samples(86%)contained two to six pathogen ***,the less detectable species tended to exist in the samples containing more detected species,and some pathogens preferentially co-occurred in diseased tissue,including ***–***–*** and ***–Calonectria ilicicola,implying potential interactions during *** LAMP detection results were confirmed by traditional isolation *** isolated strains exhibited different virulence to soybean,further implying a beneficial interaction among some pathogens.

关键词： soybean root disease fungal and oomycete plant pathogens soil-borne and seed-borne pathogens LAMP assay complex infection

Host niche, genotype, and field location shape the diversity and composition of the soybean microbiome

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Journal of Integrative Agriculture 2023年第8期22卷 2412-2425页

作者： YANG Hong-jun YE Wen-wu YU Ze SHEN Wei-liang LI Su-zhen wang Xing CHEN Jia-jia wang yuan-chao ZHENG Xiao-bo Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095P.R.China Key Laboratory of Soybean Disease and Pest Control of Ministry of Agriculture and Rural Affairs Nanjing Agricultural UniversityNanjing210095P.R.China College of Agronomy and Horticulture Jiangsu Vocational College of Agriculture and ForestryZhenjiang 212400P.R.China Suzhou Academy of Agricultural Sciences Suzhou 234000P.R.China Jining Academy of Agricultural Sciences Jining 272131P.R.China Xuzhou Academy of Agricultural Sciences Xuzhou 221131P.R.China College of Landscape Architecture Jiangsu Vocational College of Agriculture and ForestryZhenjiang 212400P.R.China

Plant-associated microbes represent a key determinant of plant fitness through acquiring nutrients,promoting growth,and resisting to abiotic and biotic ***,an extensive characterization of the bacterial and fungal microbiomes present in different plant compartments of soybean in field conditions has remained *** this study,we investigated the effects of four niches(roots,stems,leaves,and pods),four genotypes(Andou 203,Hedou 12,Sanning 16,and Zhonghuang 13),and three field locations(Jining,Suzhou,and Xuzhou)on the diversity and composition of bacterial and fungal communities in soybean using 16S and internal transcribed spacer rRNA amplicon sequencing,*** soybean microbiome significantly differed across *** genotypes explained more variation in stem bacterial community composition and leaf fungal community *** location significantly affected the composition of bacterial communities in all compartments and the effects were stronger in the root and stem than in the leaf and pod,whereas field location explained more variation in stem and leaf fungal community composition than in the root and *** relative abundances of potential soybean fungal pathogens also differed among host organs and genotypes,reflecting the niches of these microbes in the host and probably their compatibility to the host *** profiling of the microbiome composition and diversity will aid the development of plant protection technologies to benefit soybean health.

关键词： microbiome soybean plant pathogen endophytes host niche genotype field location

Genetic diversity analysis of Brassica oleracea *** SSR

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Journal of Northeast Agricultural University(English Edition) 2007年第3期14卷 202-205页

作者： TIAN yuan wang chao College of Horticulture Northeast Agricultural University Harbin 150030 China

SSR analysis on genetic diversity of 30 samples was carried out. Five primers selected from 36 primers were used to amplify 30 samples in this experiment, PCR products were separated by 6% polyacrylamide gel electrophoresis, silver staining and photographed. The results of SSR were analyzed by UPGMA clustering. The results showed that a total of 21 gene alleles were detected by 5 SSR primers. The number of alleles ranged from 2 to 5 with an average of *** range was 0.257-0.92 1, with an average of 0.543. The average coefficient of genetic similarity of SSR markers among materials was 0.432. Some of cabbage cultivars in the experiment were divided into four groups except cultivars which come from Japan.

关键词： Brassica oleracea L. SSR genetic diversity relationship

Molecular Detection of Verticillium albo-atrum by PCR Based on Its Sequences

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Agricultural Sciences in China 2005年第10期4卷 760-766页

作者： ZHANG Zheng-guang CHEN Rui-hui wang yuan-chao wang Ke-rong ZHENG Xiao-bo The Key Laboratory of Monitoring and Management of Plant Diseases and lnsects of the Ministry of Agriculture College of PlantProtection Nanjing Agricultural University Nanjing 210095 P.R.China Taizhou Entry-Exit Inspection and Quarantine Bureau Taizhou 225314 P.R. China

We developed one species-specific PCR assays for rapid and accurate detection of the pathogenic fungi Verticillium albo-atrum in diseased plant tissues and soil. Based on differences in internal transcribed spacer （ITS） sequences of Verticillium spp., a pair of species-specific primers, Vaal/Vaa2, was synthesized. After screening 17 isolates of V. alboatrum, 121 isolates from the Ascomycota, B asidiomycota, Deuteromycota, and Oomycota, the Vaal/Vaa2 primers amplified only a single PCR band of approximately 330 bp from V. albo-atrum. The detection sensitivity with primers Vaal/Vaa2 was 10 fg of genomic DNA. Using ITS1/ITS4 as the first-round primers, combined with Vaa1/Vaa2, the nested PCR procedures were developed, and the detection sensitivity increased 1 000-fold to 10 ag. The detection sensitivity for the soil pathogens was 100-conidiag^-1 soil. The PCR-based methods developed here could simplify both plant disease diagnosis and pathogen monitoring as well as guide plant disease management.

关键词： Molecular detection Verticillium albo-atrum PCR

Resistance Evaluation of Soybean Germplasm from Huanghuai Region to Phytophthora Root Rot

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Agricultural Sciences in China 2011年第2期10卷 246-251页

作者： TANG Qing-hua CUI Lin-kai LI De-long DAI Ting-ting YIN Wei-xiao DONG Sha-meng XING Han ZHENG Xiao-bo wang yuan-chao Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture/Nanjing Agricultural UniversityNanjing 210095P.R.China National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095P.R.China

The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and Rps1k that soybean cultivars or lines may *** formula of 125 Phytophthora sojae isolates were determined using the hypocotyls inoculation technique,the strains,which includ 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars or lines and resistant genes were analyzed using the gene postulating *** reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars or lines probably carried gene Rps1c and no cultivar may carry genes Rps1a or Rps1k.A few of soybean cultivars or lines from Huanghuai Region carry Rps genes Rps1a,Rps1c and Rps1k and tend to infect by ***,so resistant cultivars or lines need to be bred and popularized actively.

关键词： Phytophthora sojae differential strains soybean Phytophthora root rot resistance gene

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论权德舆的碑志散文

四川师范大学学报（社会科学版） 2006年第5期33卷 88-91页

作者：王朝源四川师范大学文学院成都610068

权德舆是中唐贞元、元和间文坛上十分活跃的人物,也是较早提倡革正文体的作家之一。其文学成绩主要在碑志文这类应用文体,其碑志散文不只是单纯用应用文的笔法去写,在取材、组织、构思、语言等方面更接近于文学散文的写法,大都写得温润... 详细信息

权德舆是中唐贞元、元和间文坛上十分活跃的人物,也是较早提倡革正文体的作家之一。其文学成绩主要在碑志文这类应用文体,其碑志散文不只是单纯用应用文的笔法去写,在取材、组织、构思、语言等方面更接近于文学散文的写法,大都写得温润弘博,自然流畅。

关键词：权德舆碑志文夹叙夹议温润弘博文明意精

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互联网工资溢价效应的阶层差异

社会学评论 2019年第2期7卷 27-41页

作者：王元超中国人民大学社会学理论与方法研究中心

本研究基于CFPS2014数据,采用倾向值加权法,探究互联网工资溢价效应的阶层差异,旨在探索数字不平等与现实社会不平等相互作用的机制。研究发现,互联网应用通过技术效应与资本效应两个路径提高了个人工资收入。技术效应与资本效应均具有... 详细信息

本研究基于CFPS2014数据,采用倾向值加权法,探究互联网工资溢价效应的阶层差异,旨在探索数字不平等与现实社会不平等相互作用的机制。研究发现,互联网应用通过技术效应与资本效应两个路径提高了个人工资收入。技术效应与资本效应均具有阶层差异,因此互联网工资溢价效应具有阶层差异:中地位阶层的互联网工资溢价效应显著高于低地位阶层,支持了资源强化理论的假设。但研究同时发现,高地位阶层的互联网工资溢价效应并不显著高于中、低地位阶层,即支持了资源替代理论关于互联网工资溢价效应存在阈值的论述。这说明互联网工资溢价效应与阶层的关系呈倒"U"型,将拉大中—低地位阶层间收入差距,缩小中—高地位阶层间收入差距。

关键词：互联网工资溢价阶层差异数字不平等倾向值加权

碘酸钾-5-溴-(2-吡啶偶氮)-二乙氨基酚体系催化动力学光度法测定痕量对苯二酚

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冶金分析 2008年第6期28卷 53-56页

作者：王园朝项青雅杭州师范大学材料与化学化工学院浙江杭州310012 杭州师范大学材料与化学化工学院

在H2SO4介质中和室温条件下,痕量对苯二酚能灵敏地催化碘酸钾氧化5-溴-(2-吡啶偶氮)-二乙氨基酚(5-Br-PADAP)的褪色反应,且褪色程度与对苯二酚含量在一定范围内成正比,据此建立了动力学催化光度法测定痕量对苯二酚的方法。以457 nm为分... 详细信息

在H2SO4介质中和室温条件下,痕量对苯二酚能灵敏地催化碘酸钾氧化5-溴-(2-吡啶偶氮)-二乙氨基酚(5-Br-PADAP)的褪色反应,且褪色程度与对苯二酚含量在一定范围内成正比,据此建立了动力学催化光度法测定痕量对苯二酚的方法。以457 nm为分析波长,对苯二酚在0.02~1.5μg/mL范围内呈线性关系,方法的检出限为2.61×10-6g/L。方法用于显影废液中对苯二酚含量的测定时,间苯二酚、米吐尔、溴化钾、Ag+等在实验条件下无干扰,但SO32-,NO2-有干扰,通过加入3.0 mol/L H2SO4加热可除去,测得结果的相对标准偏差为2.36%,加标回收率在98.3%~102.5%之间;方法同时测定工业废水中对苯二酚的相对标准偏差为2.01%,加标回收率在99.1%~103.3%之间。

关键词：对苯二酚 5-溴-(2-吡啶偶氮)-二乙氨基酚(5-Br-PADAP) 碘酸钾催化光度法