检索结果-南通市图书馆

Necessity and indications of invasive treatment for Budd-Chiari syndrome

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2011年第3期10卷 254-260页

作者： Department of General Surgery (Fu Y,Sun YL,Ma XX,Xu PQ,Feng LS,Tang Z and Luo CH),Institute of Hepatic Vascular Disease (Sun YL),Department of Radiological Intervention (Guan S and wang zw),First Affiliated Hospital,Zhengzhou University School of Medicine,Zhengzhou 450052,China Department of General Surgery First Affiliated Hospital Zhengzhou University School of Medicine Institute of Hepatic Vascular Disease First Affiliated Hospital Zhengzhou University School of Medicine

BACKGROUND:The development of collaterals in Budd-Chiari syndrome has been described and these collaterals play an important role in the presentation of this *** collaterals are diagnostic and their use in management strategy has never been *** study aimed to investigate the indications,feasibility and necessity of invasive treatment for patients with Budd-Chiari syndrome and to determine whether such a strategy is necessary for optimal ***:Twenty-nine patients who had been treated at our unit were enrolled in this *** on physical and biochemical examination,and hemodynamic compensation by collaterals,18 patients underwent radiological intervention (group A),while the other 11 had no invasive treatment (group B).The related hemodynamic parameters were acquired when percutaneous angiography was ***:In group A,all patients underwent successfully inferior vena cava (IVC) balloon angioplasty with or without *** patients also underwent hepatic vein *** these patients,the mean IVC pressure before and after treatment was statistically different (29.3±9.2 vs 15.1±4.6 mmHg,P0.05).Median follow-up was 32.3 months (mean 21.3 months;range 3-61 months).In the course of follow-up,the patients in group A survived with good systemic status except for re-stenosis in one patient who underwent re-canalization of the *** group B,10 patients had good systemic status except one patient who had a meso-caval shunt because of ***:The rationale of 'early diagnosis and early treatment' is not suitable for all patients with Budd-Chiari *** survival can be achieved in some patients without invasive treatment,who are completely compensated by rich ***

关键词： Budd-Chiari syndrome treatment indication collaterals

缺血预处理程序中心肌环磷酸腺苷含量及环磷酸腺苷依赖蛋白激酶活性的变化

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究与临床康复 2007年第29期11卷 5725-5728页

作者：韩宏光汪曾炜张南滨朱洪玉王辉山姜辉解放军第四军医大学西京医院心血管外科陕西省西安市710032

目的:制备大鼠在体缺血再灌注模型,观察缺血预处理程序中心肌环磷酸腺苷含量及环磷酸腺苷依赖蛋白激酶活性的变化。方法:实验于2005-03/2006-10在解放军沈阳军区总医院医学实验动物中心和全军心血管研究所实验室完成。实验分组:选用健... 详细信息

目的:制备大鼠在体缺血再灌注模型,观察缺血预处理程序中心肌环磷酸腺苷含量及环磷酸腺苷依赖蛋白激酶活性的变化。方法:实验于2005-03/2006-10在解放军沈阳军区总医院医学实验动物中心和全军心血管研究所实验室完成。实验分组:选用健康雌性SD大鼠36只,根据预适应程序分为第1,2,3次缺血,第1,2,3次再灌注,每一时间点6只大鼠。实验过程:用手术套管法造成左冠状动脉主干缺血及再灌注。所有实验动物在实验程序结束后,取出心脏迅速置液氮保存备用。实验评估:用放射免疫法测环磷酸腺苷水平,生化法测环磷酸腺苷依赖蛋白激酶活性变化。结果:36只大鼠均进入结果分析。①环磷酸腺苷含量:第1次再灌注组低于第1次缺血组[(0.325±0.015),(0.395±0.024)pmol/g,t=6.06,P<0.001],第2次再灌注组低于第2次缺血组[(0.523±0.017),(0.708±0.067)pmol/g,t=6.56,P<0.001],第3次再灌注组低于第3次缺血组[(0.567±0.031),(0.712±0.038)pmol/g,t=7.24,P<0.001]。②环磷酸腺苷依赖蛋白激酶活性:第1次再灌注组低于第1次缺血组[(10.115±1.000),(16.351±0.849)pkat/g,t=11.12,P<0.001],第2次再灌注组低于第2次缺血组[(11.877±2.213),(14.869±0.619)pkat/g,t=3.31,P<0.01],第3次再灌注组低于第3次缺血组[(11.745±0.987),(14.766±0.329)pkat/g,t=7.09,P<0.001]。③缺血预处理程序中心肌环磷酸腺苷含量及环磷酸腺苷依赖蛋白激酶活性随缺血及再灌注呈周期性波动。在5min缺血预处理时表现为明显增高,而在间隔的再灌注程序中恰呈相反改变,有明显下降的趋势。结论:环磷酸腺苷及环磷酸腺苷依赖蛋白激酶的周期性波动变化可能是激发心肌缺血预处理的机制之一,环磷酸腺苷可能在预处理保护作用中起一些作用。

关键词：缺血预处理环磷酸腺苷环磷酸腺苷依赖蛋白激酶大鼠

Cell reprogramming therapy for Parkinson’s disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第11期19卷 2444-2455页

作者： Wenjing Dong Shuyi Liu Shangang Li Zhengbo wang State Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunmingYunnan ProvinceChina Yunnan Key Laboratory of Primate Biomedical Research KunmingYunnan ProvinceChina

Parkinson’s disease is typically characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars *** studies have been performed based on the supplementation of lost dopaminergic neurons to treat Parkinson’s *** initial strategy for cell replacement therapy used human fetal ventral midbrain and human embryonic stem cells to treat Parkinson’s disease,which could substantially alleviate the symptoms of Parkinson’s disease in clinical ***,ethical issues and tumor formation were limitations of its clinical *** pluripotent stem cells can be acquired without sacrificing human embryos,which eliminates the huge ethical barriers of human stem cell *** widely considered neuronal regeneration strategy is to directly reprogram fibroblasts and astrocytes into neurons,without the need for intermediate proliferation states,thus avoiding issues of immune rejection and tumor *** induced pluripotent stem cells and direct reprogramming of lineage cells have shown promising results in the treatment of Parkinson’s ***,there are also ethical concerns and the risk of tumor formation that need to be *** review highlights the current application status of cell reprogramming in the treatment of Parkinson’s disease,focusing on the use of induced pluripotent stem cells in cell replacement therapy,including preclinical animal models and progress in clinical *** review also discusses the advancements in direct reprogramming of lineage cells in the treatment of Parkinson’s disease,as well as the controversy surrounding in vivo *** findings suggest that cell reprogramming may hold great promise as a potential strategy for treating Parkinson’s disease.

关键词： animal models astrocytes autologous cell reprogramming cell therapy direct lineage reprogramming dopaminergic neurons induced pluripotent stem cells non-human primates Parkinson’s disease

Human umbilical cord mesenchymal stem cell-derived exosomes loaded into a composite conduit promote functional recovery after peripheral nerve injury in rats

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第4期19卷 900-907页

作者： Haoshuai Tang Junjin Li Hongda wang Jie Ren Han Ding Jun Shang Min wang Zhijian Wei Shiqing Feng Department of Othopaedics Tianjin Medical University General Hospital International Science and Technology Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal Cord InjuryDepartment of OrthopedicsTianjin Medical University General Hospital Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment Tianjin Lung Cancer InstituteTianjin Medical University General Hospital Department of Othopaedics Qilu Hospital of Shandong UniversityShandong University Centre for OthopedicsAdvanced Medical Research InstituteShandong University Orthopedic Research Center of Shandong University&Advanced Medical Research Institute Cheeloo College of MedicineShandong University

Complete transverse injury of peripheral nerves is challenging to *** secreted by human umbilical cord mesenchymal stem cells are considered to play an important role in intercellular communication and regulate tissue *** previous studies,a collagen/hyaluronic acid sponge was shown to provide a suitable regeneration environment for Schwann cell proliferation and to promote axonal *** three-dimensional(3D)composite conduit contains a collagen/hyaluronic acid inner sponge enclosed in an electrospun hollow poly(lactic-co-glycolic acid)***,whether there is a synergy between the 3D composite conduit and exosomes in the repair of peripheral nerve injury remains *** this study,we tested a comprehensive strategy for repairing long-gap(10 mm)peripheral nerve injury that combined the 3D composite conduit with human umbilical cord mesenchymal stem cell-derived *** effectiveness was evaluated by sciatic functional index,sciatic nerve compound muscle action potential recording,recovery of muscle mass,measuring the cross-sectional area of the muscle fiber,Masson trichrome staining,and transmission electron microscopy of the regenerated nerve in *** results showed that transplantation of the 3D composite conduit loaded with human umbilical cord mesenchymal stem cell-derived exosomes promoted peripheral nerve regeneration and restoration of motor function,similar to autograft *** CD31-positive endothelial cells were observed in the regenerated nerve after transplantation of the loaded conduit than after transplantation of the conduit without exosomes,which may have contributed to the observed increase in axon regeneration and distal nerve ***,the use of a 3D composite conduit loaded with human umbilical cord mesenchymal stem cell-derived exosomes represents a promising cell-free therapeutic option for the treatment of peripheral nerve injury.

关键词： axon growth collagen exosome human umbilical cord mesenchymal stem cells hyaluronic acid muscular atrophy nerve guidance conduits peripheral nerve regeneration

Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第7期35卷 222-226页

作者： Rui wang He Qi Haijiao Zheng Qiong Jia College of Chemistry Jilin UniversityChangchun 130012China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun 130012China

Metal-organic frameworks(MOFs)combined with specific ligands are highly adaptable smart materials that can respond to external and physiological *** this study,we introduced a pyridinyl zwitterionic ligand with light/pH dual response into magnetic MOF composite(Fe_(3)O_(4)@zw-MOF)for enrichment of phosphorylated peptides for the first *** introduction of the developed ligand gives MOF material dual response *** stimulation affects the generation/disappearance of free radicals of the pyridine derivative,resulting in a change in the charge gradient of the zwitterion,and zwitterion can also regulate the p H of the solution by adding acid or ***,the reversible capture and release of phosphorylated peptides can be easily achieved by adjusting light and *** established phosphorylated peptide enrichment platform exhibits high sensitivity(detection limit of 1 fmol),high selectivity(β-casein:BSA,1:1000),and good reusability(7 cycles).In addition,the method was applied to the enrichment of phosphorylated peptides in complex systems(non-fat milk and human serum),demonstrating the feasibility of this method for phosphoproteom *** conclusion,the synthesized Fe_(3)O_(4)@zw-MOF is a promising MOF material,which provides the possibility to advance the application of responsive MOFs materials in proteomics.

关键词： Light pH Dual-response Metal-organic frameworks Phosphorylated peptide

同方期刊数据库

Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 1995年第3期13卷 246-250页

作者： XU, ZS YANG, zw wang, Y CHINESE ACAD SCI SHANGHAI INST ORGAN CHEMSHANGHAI 200032PEOPLES R CHINA

Large scale synthesis of oligoribonucleotides has been successfully performed on PEG support by the phosphoramidite approach using t-butyldimethylsilyl to protect the 2'-hydroxyl group of ribonucleoside. By means of this procedure, the dodecamer r(AGUGGUCUUUGU) was synthesized in 98.1% average coupling yield, and 55 mg pure product was obtained from one gram of functionalized PEG.

关键词： OLIGORIBONUCLEOTIDES LARGE SCALE SYNTHESIS PHOSPHORAMIDITE APPROACH PEG SUPPORT

Three New 6,7-Seco-Ent-Kaurane Diterpenoids from Isodon Eriocalyx

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第5期8卷 421-424页

作者： wang, J Lin, zw Sun, HD ACAD SINICA KUNMING INST BOTLAB PHYTOCHEMKUNMING 650204PEOPLES R CHINA

From the dried leaves of I. eriocalyx, three new 6,7-seco- ent-kaurane diterpenoids epi-eriocalyxin A (2), maoecrystal N (3) and maoecrystal O (4) were isolated. Meanwhile, the structure of a known constituent eriocal... 详细信息

关键词： wang Three New 6,7-Seco-Ent-Kaurane Diterpenoids from Isodon Eriocalyx

Exploring the molecular landscape of NNK-induced transformation:A comprehensive genome-wide CRISPR/Cas9 screening

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第4期11卷 42-44页

作者： Trang Dinh Mira Rahm Zhenghe wang Christopher McFarland Athar Khalil Department of Genetics and Genome Sciences Case WesternReserve UniversityClevelandOH44106USA

Lung cancer is the leading cause of cancer-related deaths worldwide,with an estimated 1.8 million deaths in 2020 alone.1 While several risk factors are associated with lung cancer,smoking remains the most significant environmental cause of the disease.1 One of the most potent carcinogens in tobacco smoke is 4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone(NNK),which is produced from nicotine through a series of metabolic reactions.

关键词： CRISPR/Cas9 NNK lung

Five New Flavonoids from Bidens Pilosa L. Var. Vadiata SCH.-BIP

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第7期8卷 599-602页

作者： wang, J Yang, H Lin, zw Sun, HD ACAD SINICA KUNMING INST BOTLAB PHYTOCHEMKUNMING 650204PEOPLES R CHINA YUNNAN UNIV DEPT CHEMKUNMING 650091PEOPLES R CHINA

Five new flavonoids were isolated from the aerial parts of Bidens pilosa L. var. vadiata Sch.-Bip.. Their structures were established on the basis of spectroscopic methods.

关键词： Vadiata SCH OAC BIP Five New Flavonoids from Bidens Pilosa L Var