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高温胁迫下厚壳贻贝(Mytilus coruscus)消化腺代谢组学研究

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海洋与湖沼 2024年第1期55卷 171-181页

作者：郭锋王玉霞董震宇姚荣辉王伟峰叶莹莹严小军郭宝英国家海洋设施养殖工程技术研究中心浙江舟山316022 浙江海洋大学海洋科学与技术学院浙江舟山316022

在全球变暖的背景下,海水温度不断升高、海洋暖化逐渐加剧,高温严重影响着海洋生物的各种生命过程,但对于海洋双壳贝类如何应对热应激的研究仍然不足。为此,开展了以18℃(CT)为对照组在26℃(ST)和33℃(HT)下对厚壳贻贝消化腺组织进行了... 详细信息

在全球变暖的背景下,海水温度不断升高、海洋暖化逐渐加剧,高温严重影响着海洋生物的各种生命过程,但对于海洋双壳贝类如何应对热应激的研究仍然不足。为此,开展了以18℃(CT)为对照组在26℃(ST)和33℃(HT)下对厚壳贻贝消化腺组织进行了急性热胁迫下的代谢组学分析,以便于研究其代谢反应。采用LC-MS/MS技术,并结合生物信息分析手段对差异代谢物进行筛选,并分析确定相关的代谢通路的变化,共有2532种代谢物在厚壳贻贝消化腺中被鉴定。KEGG富集分析用于探索差异代谢物的潜在代谢途径,共有29条代谢通路被显著富集,与对照组相比,ST组显著富集于牛磺酸和次牛磺酸代谢、神经活性配体-受体相互作用、鞘脂类代谢和视黄醇代谢等代谢通路;HT组显著富集于酪氨酸代谢、亚油酸代谢、丙氨酸新陈代谢、酪氨酸代谢、色氨酸代谢、苯丙氨酸代谢等代谢通路。研究结果显示,厚壳贻贝消化腺主要通过调节色氨酸代谢、酪氨酸代谢、鞘脂代谢、苯丙氨酸代谢、氧化磷酸化,脂肪酸、赖氨酸降解等信号通路应对热应激,从而帮助维持身体内部环境的稳定状态。上述研究为多视角探究厚壳贻贝耐热机制与应对环境中温度变化的适应性进化提供理论基础。

关键词：厚壳贻贝(Mytilus coruscus) 消化腺高温胁迫代谢组学

Littlest Higgs Model and Higgs Boson Associated Production with Top Quark Pair at High Energy Linear e^＋e^- Collider

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Communications in Theoretical Physics 2006年第3期45卷 511-516页

作者： YUE Chong-Xing wang wei ZHANG feng Department of Physics Liaoning Normal University Dalian 116029 China

In the parameter space allowed by the electroweak precision measurement data, we consider the contributions of the new particles predicted by the littlest Higga model to the Higgs hoson associated production with top quark pair in the future high energy linear e^＋e^- collider （ILU）. We find that the contributions mainly come from the new gauge bosons ZH and BH. For reasonable values of the free parameters, the absolute value of the relative correction parameter δσ/σ^SM can be signiticanly large, which might be observed in the future ILU experiment with √S = 800 GeV.

关键词： littlest Higgs model Higgs boson new gauge bosons

Relationship between dispersibility of ZrO2 nanoparticles in Ni-ZrO2 electroplated nanocomposite coatings and mechanical properties of nanocomposite coatings

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中国有色金属学会会刊：英文版 2004年第Z1期14卷 186-189页

作者： wang wei HOU feng-yan GUO He-tong School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

Ni-ZrO2 nanocomposite coatings with monodispersed ZrO2 nanoparticles were prepared from the composite plating bath containing dispersant under DC electrodeposition condition. It is found that the morphology, orientation and hardness of the composite coating with monodispersed ZrO2 nanoparticles have lots of difference from the composite coating with agglomerated ZrO2 nanoparticles and pure nickel coating. Especially, the result of hardness shows that only a very low volume fraction (less than 1%) of monodispered ZrO2 nanoparticles in Ni-ZrO2 composite coatings will result in higher hardness of the coating. The hardness of Ni-ZrO2 nanocomposite coatings with monodispersed and agglomerated ZrO2 nanoparticles are HV 529 and HV 393, respectively. The hardness value of the former composite coatings is over 1.3 times higher than that of the later. All these composite coatings are 2 - 3 times higher than that of pure nickel plating (HV 207) prepared under the same conditions.

关键词： Nanocomposites electroplating coating metal matrix composites Ni-ZrO2

Synthesis and Crystal Structure of 3-Nitrophthalic Acid·3-methyl-4-nitropyridine N-Oxide Adducts

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Chinese Journal of Structural Chemistry 2006年第8期25卷 923-926页

作者： wang Yi feng wei XUE Lin ZHENG Ji-Min Department of Chemistry Nankai University Tianjin 300071 China

The title compound, a 1：1 molecular adduct of 3-nitrophthalic acid and 3-methyl- 4-nitropyridine N-oxide （PPOM）, has been synthesized and characterized by X-ray single-crystal structure analysis. It crystallizes in triclinic, space group PI with α = 7.6076（15）, b = 7.8180（16）, c = 14.546（3）A, α= 93.90（3）, β = 97.21（3）, γ= 114.43（3）°, C14H1N3O9, Mr = 365.26, Z = 2, V = 774.6（3） A^3, Dc = 1.566 g/m^3,μ（MoKa） = 0.134 mm^-1, F（000） = 376, R = 0.0538 and wR = 0. 1460 for 885 observed reflections （1 〉 2σ（I））. The protons of the carbonylic acids in the molecule are not transferred and the O-H…O and C-H…O hydrogen bonds form zigzag chains in the molecule.

关键词： crystal structure synthesis 3-methyl-4-nitropyridine N-oxide adduct

Effects of short-term osmotic stress on leaf hydraulic conductivity and ZmPIPs mRNA accumulation in maize seedlings

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Journal of Integrative Agriculture 2016年第11期15卷 2497-2506页

作者： wang wei-feng ZONG Yu-zheng ZHANG Sui-qi State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau/Institute of Soil and Water Conservation Northwest A & F University College of Forestry Shanxi Agricultural University College of Agriculture Shanxi Agricultural University

Plants maintain water balance by varying hydraulic properties, and plasma membrane intrinsic proteins（PIPs） may be involved in this process. Leaf xylem and root hydraulic conductivity and the m RNA contents of four highly expressed Zm PIP genes（Zm PIP1;1, Zm PIP1;2, Zm PIP2;2, and Zm PIP2;5） in maize（Zea mays） seedlings were investigated. Under well-watered conditions, leaf hydraulic conductivity（K_（leaf）） varied diurnally and was correlated with whole-plant hydraulic conductivity. Similar diurnal rhythms of leaf transpiration rate（E）, K_（leaf） and root hydraulic conductivity（K_（root）） in well-watered plants are important for maintaining whole-plant water balance. After 2 h of osmotic stress treatment induced by 10% polyethylene glycol 6000, the K_（root） of stressed plants decreased but K_（leaf） increased, compared with well-watered plants. The m RNA contents of four Zm PIPs were significantly up-regulated in the leaves of stressed plants, especially for Zm PIP1;2. Meanwhile, Zm PIP2;5 was significantly down-regulated in the roots of stressed plants. After 4 h of osmotic stress treatment, the E and leaf xylem water potentials of stressed plants unexpectedly increased. The increase in K_（leaf） and a partial recovery of K_（root） may have contributed to this process. The m RNA content of Zm PIP1;2 but not of the other three genes was up-regulated in roots at this time. In summary, the m RNA contents of these four Zm PIPs associated with K_（leaf） and K_（root） change in maize seedlings during short-term osmotic stress, especially for Zm PIP1;2 and Zm PIP2;5, which may help to further reveal the hydraulic resistance adjustment role of Zm PIPs.

关键词： maize short-term osmotic stress hydraulic conductivity ZmPIP

太白茶HPLC指纹图谱的建立及UHPLC-QExactive Focus MS/MS联用分析

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药物分析杂志 2024年第4期44卷 585-593页

作者：李莎莎李雅娟王晓婷毛阿娟李凡李芳张红王卫锋陕西省中医药研究院西安710003 陕西中医药大学咸阳712046

目的:建立太白茶药材高效液相色谱(HPLC)指纹图谱分析方法,并运用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q Exactive Focus MS/MS)法对太白茶中的特征峰进行定性分析。方法:采用Agilent TC-C18(250 mm×4.6 mm,5μm)色谱... 详细信息

目的:建立太白茶药材高效液相色谱(HPLC)指纹图谱分析方法,并运用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q Exactive Focus MS/MS)法对太白茶中的特征峰进行定性分析。方法:采用Agilent TC-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min^(-1),柱温30℃,检测波长254 nm,进样量10μL。采用“中药色谱指纹图谱相似度评价系统2012年版”,建立指纹图谱并进行相似度评价。化学成分分析选用UHPLC-Q Exactive Focus MS/MS技术,采用Waters Acquity UPLC BEH C18(50 mm×2.1 mm,1.7μm)色谱柱,以乙腈(A)-0.1%甲酸水(B)为流动相,梯度洗脱,流速0.3 mL·min^(-1),柱温30℃;质谱条件为电喷雾离子源(ESI),负离子模式扫描,扫描范围m/z 80~1200。结果:建立的太白茶特征指纹图谱中,标定共有峰8个,10批太白茶药材相似度均≥0.9,各批次之间共有峰的相对保留时间RSD均<0.5%。对8个共有峰中的4个峰进行了结构指认,依次为鳞片衣酸、羊角衣酸、thamnoliadepsides B、坝巴酸。结论:所建立的HPLC指纹图谱方法稳定可靠,专属性好,结合UHPLC-Q-Exactive Focus-MS/MS的定性分析,可用于太白茶药材的整体质量综合评价。

关键词：太白茶高效液相色谱指纹图谱质量评价超高效液相色谱-四极杆/静电场轨道阱高分辨质谱定性分析

Synthesis and Crystal Structure of Dehydroandrographolide Dipolycyclophosphate

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Chinese Journal of Structural Chemistry 2005年第10期24卷 1198-1202页

作者： XU Hai-wei wang Jun-feng LIU Hong-Min ZHANG Jian-Ye New Drug Research ＆ Development Center Zhengzhou University Zhengzhou 450052 China

The title compound was synthesized and characterized by IR, NMR, HRSI-MS and MS, and its crystal structure was determined by single-crystal X-ray diffraction. The crystal is of orthorhombic system （C40H52O11P2, Mr= 770.76）, space group P21212, with a = 22.562（5）, b = 29.224（6）, c = 7.1953（14） A, V = 4744.2（16） A^3, Z = 4, Dc = 1.079 g/cm^3, F（000） = 1640 and μ = 0.141 mm^-1. The final R = 0.0758 and wR = 0.1778 for 2794 observed reflections with I 〉 20（I）. Intermolecular hydrogen bonds are found between the O atom of carbonyl group and H atoms of olefinic carbon. The absolute configuration of this molecule was confirmed by comparison with that of the original material.

关键词： crystal structure andrographolide cyciophosphate synthesis

Harmonic coordinates for real-time image cloning

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第9期11卷 690-698页

作者： Rui wang wei-feng CHEN Ming-hao PAN Hu-jun BAO State Key Laboratory of CAD &CG Zhejiang University Hangzhou 310027 China

Traditional gradient domain seamless image cloning is a time consuming task,requiring the solving of Poisson's equations whenever the shape or position of the cloned region ***,a more efficient alternative,the mean-value coordinates(MVCs) based approach,was proposed to interpolate interior pixels by a weighted combination of values along the ***,this approach cannot faithfully preserve the gradient in the cloning *** this paper,we introduce harmonic cloning,which uses harmonic coordinates(HCs) instead of MVCs in image *** from the non-negativity and interior locality of HCs,our interpolation generates a more accurate harmonic field across the cloned region,to preserve the results with as high a quality as with Poisson ***,with optimizations and implementation on a graphic processing unit(GPU),we demonstrate that,compared with the method using MVCs,our harmonic cloning gains better quality while retaining real-time performance.

关键词： Seamless cloning Poisson’s equation Harmonic coordinates(HCs) Mean-value coordinates(MVCs) GPU acceleration

Influence of Nb on Microstructure and Property of Low-Carbon Mn-Series Air-Cooled Bainitic Steel

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Journal of Iron and Steel Research International 2010年第1期17卷 49-53页

作者： wang Yong-wei feng Chun XU feng-yun BAI Bing-zhe FANG Hong-sheng Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

The effect of Nb on the microstructure evolution of low-carbon Mn-series air cooled bainitic steels has been studied by using thermomeehanical simulation machine, SEM, and TEM observation. The results show that the amount of ferrites is relatively higher in the steel without Nb than that of Nb-bearing steel under same deformation conditions, and the ferrites in Nb-bearing steel are more finer. The mechanical properties of the Nb-bearing steel are higher than those of the steel without Nb at the same finishing rolling temperature （FRT）, and the toughness of the Nb- bearing steel is about 100J higher than that of the steel without Nh at the finishing rolling temperature 750℃. The toughness of Nb microalloyed steel will be improved more effectively than that of the steel without Nb with the decrease of FRT.

关键词： Mn-series bainitic steel grain boundary allotriomorphic ferrite finishing rolling temperature Nb microalloyed steel toughness