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Regulation of innate immune responses by rabies virus

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Animal Models and Experimental Medicine 2022年第5期5卷 418-429页

作者： Haili Zhang Jingbo Huang Yumeng Song Xingqi Liu Meichen Qian Pei Huang Yuanyuan Li Ling Zhao hualei wang State Key Laboratory for Zoonotic Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of Zoonosisand College of Veterinary MedicineJilin UniversityChangchunChina State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhanChina

Rabies virus(RABV)is an infectious and neurotropic pathogen that causes rabies and infects humans and almost all warm-blooded animals,posing a great threat to people and public *** is well known that innate immunity is the critical first line of host defense against viral *** monitors the invading pathogens by recognizing the pathogen-associated molecular patterns and danger-associated molecular patterns through pattern-recognition receptors,leading to the production of type I interferons(IFNα/β),inflammatory cytokines,and chemokines,or the activation of autophagy or apoptosis to inhibit virus *** the case of RABV,the innate immune response is usually triggered when the skin or muscle is bitten or ***,RABV has evolved many ways to escape or even hijack innate immune response to complete its own replication and eventually invades the central nervous system(CNS).Once RABV reaches the CNS,it cannot be wiped out by the immune system or any ***,a better understanding of the interplay between RABV and innate immu-nity is necessary to develop effective strategies to combat its ***,we review the innate immune responses induced by RABV and illustrate the antagonism mechanisms of RABV to provide new insights for the control of rabies.

关键词： apotosis autophagy infectious disease and host defense innate immunity and inflammation rabies virus

基于品质性状与矿质元素含量的多花黄精种源多样性分析及评价

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山东农业科学 2024年第6期56卷 55-64页

作者：王灿彬潘克琴王华磊罗春丽李金玲陈松树李丹丹梁瑾刘红昌罗夫来黄明进贵州大学农学院/贵州省药用植物繁育与种植重点实验室贵州贵阳550025

为研究多花黄精种质资源品质性状与矿质元素的遗传多样性并筛选优质种质,本试验以收集自国内的43份多花黄精种质为材料,测定其7个品质性状与15种矿质元素含量并进行多样性分析、相关性分析、主成分分析、聚类分析及隶属函数法综合评价... 详细信息

为研究多花黄精种质资源品质性状与矿质元素的遗传多样性并筛选优质种质,本试验以收集自国内的43份多花黄精种质为材料,测定其7个品质性状与15种矿质元素含量并进行多样性分析、相关性分析、主成分分析、聚类分析及隶属函数法综合评价。结果表明,43份种质多糖、总灰分、醇溶性浸出物、水分、部分重金属及有害元素含量均符合2020版《中华人民共和国药典》的标准;品质性状的变异系数在10.4%~46.7%之间,遗传多样性指数在1.600~2.015之间;矿质元素含量的变异系数在23.6%~92.7%之间,遗传多样性指数在1.526~2.006之间。相关性分析显示,品质性状间、矿质元素间、品质性状与矿质元素间共76对指标之间达到显著(P<0.05)或极显著(P<0.01)相关水平,矿质元素之间相关关系复杂但整体协同作用较强,矿质元素对品质性状则抑制作用较强。主成分分析将11种矿质元素简化为3个主成分,累计方差贡献率为66.71%,筛选出K、Zn、Fe、P作为评价多花黄精种质资源的特征元素。系统聚类分析在距离为100时将43份多花黄精种质资源分为4个类群,隶属函数法综合评价与聚类分析的结果基本一致,其中排名前十的优质种质为S33、S27、S29、S28、S31、S10、S17、S36、S32、S20。综上表明,多花黄精种质的品质性状与矿质元素含量变异明显,具有丰富的遗传多样性。本结论可为优质多花黄精种质选育提供理论参考和依据。

关键词：多花黄精种质资源品质性状矿质元素遗传多样性综合评价

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Genetically engineered bacterial-like particles induced specific cellular and humoral immunity as effective tick-borne encephalitis virus vaccine

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聚集体(英文)

聚集体(英文) 2023年第3期4卷 175-183页

作者： Mengyao Zhang Hongli Jin Yuanyuan Li Cuicui Jiao Pei Huang Yujie Bai Zhiyuan Gong Haili Zhang Shunjie Liu hualei wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchunChina

Tick-borne encephalitis(TBE)is a natural focal disease with fatal encephalitis induced by tick-borne encephalitis virus(TBEV),seriously threatening human and public *** of TBE depends on vaccination with inactivated vaccine,which requires high cost and multiple ***,we construct genetically engineered bacterial-like particles(BLPs)as an effective TBEV vaccine with simplified immunizations and improved immune *** TBEV BLPs involve the combination of the gram-positive enhancer matrix from Lactococcus lactis,and TBEV envelope(E)protein expressed by genetically engineered recombinant *** prepared TBEV BLPs can effectively stimulate the activation of dendritic cells to present the TBEV E proteins to T and B cells,leading to strong and durable cellular and humoral immune responses in ***,the serum levels of specific IgG antibodies in mice remain about 10^(6)at 6 months after the secondary ***,the TBEV BLPs can be used as a potent vaccine candidate,laying the foundation for developing novel TBEV genetically engineered vaccines.

关键词： bacterial-like particles envelope protein genetically engineered vaccines gram-positive enhancer matrix tick-borne encephalitis virus

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肥水处理对一年生何首乌块根产量的影响

特产研究 2024年第5期46卷 30-35页

作者：胡继田李金玲王华磊罗春丽刘红昌赵致安顺市农业科学院贵州安顺562109 贵州大学贵州贵阳550025 贵州省药用植物繁育与种植重点(工程)实验室贵州贵阳550025

为探明一年生何首乌生长的水肥田间管理方法,以大棚盆栽何首乌为试材,用氮、磷、钾、水4因素3水平正交试验设计L^(9)(3^(4))方法,测定一年生何首乌产量及最大块根重量。结果表明,氮、磷、钾、水4个因素对何首乌块根产量的影响,其主次顺... 详细信息

为探明一年生何首乌生长的水肥田间管理方法,以大棚盆栽何首乌为试材,用氮、磷、钾、水4因素3水平正交试验设计L^(9)(3^(4))方法,测定一年生何首乌产量及最大块根重量。结果表明,氮、磷、钾、水4个因素对何首乌块根产量的影响,其主次顺序依次为土壤持水量、磷肥、氮肥和钾肥;水和磷两个因素对何首乌产量的影响达到了极显著水平,而氮与钾两个因素未达到显著水平;产量随土壤持水量的增大呈先上升后下降的趋势,最大产量出现在水因素为W_(2)时,为297.86 g/株;各处理何首乌最大块根重量在24.57~80.95 g的范围内变化;何首乌最大块根重量与其产量呈极显著正相关。一年生何首乌产量适宜土壤水分含量为65%田间持水量,磷肥单株每盆需要P_(2)O_(5)2.16 g。

关键词：何首乌肥水块根产量

Preferentially selective extraction of lithium from spent LiCoO_(2)cathodes by medium-temperature carbon reduction roasting

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International Journal of Minerals,Metallurgy and Materials 2024年第2期31卷 315-322页

作者： Daixiang Wei Wei wang Longjin Jiang Zhidong Chang hualei Zhou Bin Dong Dekun Gao Minghui Zhang Chaofan Wu School of Chemistry and Biological Engineering University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Anhui Chaoyue Environmental Protection Technology Co. LtdChuzhou 239060China Anhui Huihong Technology Co. LtdChuzhou 239060China

Lithium recovery from spent lithium-ion batteries(LIBs)have attracted extensive attention due to the skyrocketing price of *** medium-temperature carbon reduction roasting was proposed to preferential selective extraction of lithium from spent Li-CoO_(2)(LCO)cathodes to overcome the incomplete recovery and loss of lithium during the recycling *** LCO layered structure was destroyed and lithium was completely converted into water-soluble Li2CO_(3)under a suitable temperature to control the reduced state of the cobalt *** Co metal agglomerates generated during medium-temperature carbon reduction roasting were broken by wet grinding and ultrasonic crushing to release the entrained *** results showed that 99.10%of the whole lithium could be recovered as Li2CO_(3)with a purity of 99.55%.This work provided a new perspective on the preferentially selective extraction of lithium from spent lithium batteries.

关键词： spent LiCoO_(2)cathodes medium-temperature carbon reduction lithium extraction priority crystal transformation macro-scopic transport resistance

Prevention and control of zoonoses at their source:from the Chinese perspective

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Frontiers of Agricultural Science and Engineering 2014年第2期1卷 96-103页

作者： Songtao YANG Yuwei GAO Jun QIAN Quan LIU Xuexing ZHENG hualei wang Zhiping XIA Xianzhu XIA Key Laboratory of Jilin Province for Zoonosis Prevention and Control Military Veterinary InstituteAcademy of Military Medical SciencesChangchun 130122China

Zoonoses are a significant public health concern and cause considerable socioeconomic problems *** emergence of severe acute respiratory syndrome(SARS),highly pathogenic avian influenza(HPAI),and Ebola virus disease(EVD)has had a significant effect on the national economy and public health in China,and other *** review analyzed zoonotic disease issues faced by China,and the main factors contributing to the risk of zoonotic *** Chinese government has devised new strategies and has taken measures to deal with the challenges of these diseases,and the prevention and control of zoonoses at their source.A strategy that is suited to China’s national conditions,is proposed.

关键词： zoonosis prevention and control joint prevention and control

检测寨卡病毒核酸的RT-LAMP可视化方法的建立

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中国兽医学报 2024年第8期44卷 1698-1703页

作者：宋雨濛黄培金宏丽焦翠翠白玉洁张梦瑶龚志远李媛媛张海丽王化磊吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/教育部人兽共患病研究重点实验室吉林长春130062 长春西诺生物科技有限公司吉林长春130012

为建立简单、便捷、灵敏、特异的寨卡病毒(Zika virus,ZIKV)快速检测方法,本研究通过对不同时间、不同地区分离的ZIKV全基因组序列进行分析,筛选ZIKV NS5基因保守区为靶标,同时设计特异性引物及探针,结合RT-LAMP恒温扩增技术和免疫层析... 详细信息

为建立简单、便捷、灵敏、特异的寨卡病毒(Zika virus,ZIKV)快速检测方法,本研究通过对不同时间、不同地区分离的ZIKV全基因组序列进行分析,筛选ZIKV NS5基因保守区为靶标,同时设计特异性引物及探针,结合RT-LAMP恒温扩增技术和免疫层析技术,成功构建ZIKV反转录环介导等温扩增核酸可视化(RT-LAMP-VF)检测方法。结果显示,该方法具有良好的特异性和敏感性,当内、外、环引物比为FIP∶LF∶F3=4∶2∶1时,在61℃条件下恒温扩增45 min,可特异性检测到10^(2)copies/μL的RNA标准品以及2.15 pfu的ZIKV,且与其他黄病毒(日本乙型脑炎病毒、猪瘟病毒)等无交叉反应。血液组织中的其他RNA不影响RT-LAMP-VF的敏感性和特异性,表明该方法可用于临床样本ZIKV的检测。本研究建立的ZIKV RT-LAMP-VF检测方法操作简单、不需要特殊仪器设备,尤其适用于基层单位ZIKV的现场快速检测,为ZIKV病的现场快速检测和预警预报体系的建立提供技术支持与物质保障。

关键词：寨卡病毒环介导等温扩增免疫层析试纸条核酸可视化检测

An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

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Virologica Sinica 2023年第2期38卷 244-256页

作者： Haili Zhang Hongli Jin Feihu Yan Yumeng Song Jiaxin Dai Cuicui Jiao Yujie Bai Jingxuan Sun Di Liu Shen wang Mengyao Zhang Jilong Lu Jingbo Huang Pei Huang Yuanyuan Li Xianzhu Xia hualei wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun130062China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun130122China Changchun Sino Biotechnology Co. Ltd.Changchun130012China

Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and *** is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in *** virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health *** present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and *** results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in ***,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer *** SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.

关键词： SARS-CoV-2 Vaccine RBD Chimeric expression RABV

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

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Virologica Sinica 2023年第4期38卷 635-638页

作者： Pei Huang Zanheng Huang Meihui Liu Yujie Bai Hongli Jin Jingbo Huang Xingqi Liu Zhenhong Guan Ming Duan Haili Zhang Yuanyuan Li Sandra Chiu hualei wang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun130062China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei244199China

Dear Editor,Monkeypox virus(MPXV)is an enveloped double-stranded DNA virus belonging to the family Poxviridae,subfamily Chordopoxvirinae,and genus Orthopoxvirus(Hraib et al.,2022;Gong et al.,2022).MPXV forms Congo Basin clade(clade I)and West African clade(clade II)(Durski et al.,2018).Additionally,clade II consists of two subclades,clade IIa and clade IIb.

关键词： belong panel African