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蔗糖对参薯块茎形成及DamiR156/DaSPL的调控分析

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分子植物育种 2024年第7期22卷 2098-2103页

作者：庄赛伟刘铭吴晓龙梅朋飞黄小龙夏薇吴文嫱黄东益海南大学生命科学学院海口570228 海南大学热带作物学院海口570228

为了探究蔗糖对不同基因型参薯块茎形成的影响,以及对块茎发育过程中相关的DamiR156及其靶基因DaSPL的调控作用,本研究采用不同蔗糖浓度处理参薯组培苗Da40、Da70、Da207,结果表明高浓度的蔗糖能够促进Da40和Da70的块茎形成。当蔗糖浓度... 详细信息

为了探究蔗糖对不同基因型参薯块茎形成的影响,以及对块茎发育过程中相关的DamiR156及其靶基因DaSPL的调控作用,本研究采用不同蔗糖浓度处理参薯组培苗Da40、Da70、Da207,结果表明高浓度的蔗糖能够促进Da40和Da70的块茎形成。当蔗糖浓度为90 g/L时,Da40的结薯时间提早了22 d,Da70的结薯时间提早了40 d。使用RT-qPCR技术测定不同蔗糖浓度处理下Da70的DamiR156及其靶基因DaSPL6相对表达量。结果表明,在第30天,DamiR156的表达量在蔗糖浓度为70 g/L时表达量最高。在第60天(即块茎形成初期),当蔗糖浓度为0 g/L时,DamiR156的表达量最高,DaSPL6表达量最低,在6个不同蔗糖浓度处理下,DaSPL6的表达量呈现先上升后下降的趋势,DaSPL6在70 g/L时表达量最高。研究结果表明外源蔗糖可促进参薯生长并诱导提前结薯,调节DamiR156/DaSPL6的表达,这为后续研究蔗糖调控参薯块茎形成的分子机制提供了一定的理论依据。

关键词：参薯蔗糖 RT-qPCR DamiR156 DaSPL6

PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis

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World Journal of Gastroenterology 2017年第29期23卷 5345-5355页

作者： xiao-ming Liu Can-xia Xu Lin-Fang Zhang Li-Hua huang Ting-Zi Hu Rong Li Xiu-Juan xia Lin-Yong Xu Ling Luo xiao-xia Jiang ming Li Department of Gastroenterology the Third Xiangya Hospital of Central South University Center for Medical Experiments the Third Xiangya Hospital of Central South University Department of Medical Statistics Xiangya School of Public Health Central South University

AIM To clarify the mechanisms of connexin 32 (Cx32) downregulation by potential transcriptional factors (TFs) in Helicobacter pylori (H. pylori)-associated gastric carcinogenesis. METHODS Approximately 25 specimens at each developmental stage of gastric carcinogenesis [non-atrophic gastritis, chronic atrophic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma (GC)] with H. pylori infection [H. pylori (+)] and 25 normal gastric mucosa (NGM) without H. pylori infection [H. pylori (-)] were collected. After transcriptional factor array analysis, the Cx32 and PBX1 expression levels of H. pylori-infected tissues from the developmental stages of GC and NGM with no H. pylori infection were measured by real-time polymerase chain reaction (RT-PCR) and Western blot analysis. Regarding H. pylori-infected animal models, the Cx32 and PBX1 mRNA expression levels and correlation between the gastric mucosa from 10 Mongolian gerbils with long-term H. pylori colonization and 10 controls were analyzed. PBX1 and Cx32 mRNA and protein levels were further studied under the H. pylori-infected condition as well as PBX1 overexpression and knockdown conditions in vitro. RESULTS Incremental PBX1 was first detected by TF microarray in H. pylori-related gastric carcinogenesis. The identical trend of PBX1 and Cx32 expression was confirmed in the developmental stages of H. pylori-related clinical specimens. The negative correlation of PBX1 and Cx32 was confirmed in H. pylori-infected Mongolian gerbils. Furthermore, decreased PBX1 expression was detected in the normal gastric epithelial cell line GES-1 with H. pylori infection. Enforced overexpression or RNAi-mediated knockdown of PBX1 contributed to the diminished or restored Cx32 expression in GES-1 and the gastric carcinoma cell line BGC823, respectively. Finally, dual-luciferase reporter assay in HEK293T cells showed that Cx32 promoter activity decreased by 30% after PBX1 vector co-transfection, indicating PBX1 as a transcriptio

关键词： Helicobacter pylori Inflammation-carcinoma chain Connexin 32 PBX1 Carcinogenesis

电针对心肌缺血再灌注损伤大鼠心室结构和功能的影响

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针刺研究 2024年第1期49卷 6-14页

作者：吴双姜文丁雅萍刘琼琼白桦徐森磊张宏如夏铭徽顾一煌南京中医药大学针灸推拿学院养生康复学院南京210023 南通大学附属医院针灸科江苏南通226001 南京中医药大学南通附属医院脑病科江苏南通226001 南京中医药大学中医学院·中西医结合学院南京210023

目的:观察电针对心肌缺血再灌注损伤(MIRI)大鼠心室结构和功能的影响,探讨电针改善MIRI后心室重塑的潜在机制。方法:将40只雄性SD大鼠随机分为假手术组、模型组、电针组、LCZ696(阳性药对照)组,每组10只。通过冠状动脉左前降支结扎法建... 详细信息

目的:观察电针对心肌缺血再灌注损伤(MIRI)大鼠心室结构和功能的影响,探讨电针改善MIRI后心室重塑的潜在机制。方法:将40只雄性SD大鼠随机分为假手术组、模型组、电针组、LCZ696(阳性药对照)组,每组10只。通过冠状动脉左前降支结扎法建立MIRI模型。电针组取双侧“内关”进行电针治疗,每次20 min,隔日1次;LCZ696组每日按60 mg·kg^(-1)·d^(-1)的剂量予LCZ696灌胃。以上干预连续21 d。采用超声心动图检测各组大鼠左心室射血分数(EF)和短轴缩短指数(FS);酶联免疫吸附法检测各组大鼠血清中肿瘤坏死因子α(TNF-α)、血管细胞黏附分子1(VCAM-1)、细胞间黏附分子1(ICAM-1)含量;HE染色法观察大鼠心肌组织病理形态变化;Masson染色法观察大鼠心肌组织胶原沉积情况及心肌纤维化程度;荧光定量PCR法检测大鼠心肌组织中心肌纤维化标志物CollagenⅠ、CollagenⅢ、CTGF mRNA表达水平;Western blot法检测大鼠心肌组织炎性因子IL-1β、IL-18表达水平。结果:与假手术组比较,模型组大鼠左心室EF、FS降低(P<0.001);血清中TNF-α、VCAM-1、ICAM-1含量增加(P<0.001);心肌纤维排列紊乱,并伴有炎性细胞浸润,心肌组织胶原沉积增多,心肌胶原纤维面积占比升高(P<0.0001);心肌组织中CollagenⅠ、CollagenⅢ、CTGF mRNA表达水平均升高(P<0.05,P<0.01),IL-1β、IL-18表达水平升高(P<0.000 1)。与模型组比较,电针组和LCZ696组大鼠EF、FS升高(P<0.01);血清中TNF-α、VCAM-1、ICAM-1含量减少(P<0.001,P<0.01);部分心肌细胞排列紊乱,炎性细胞浸润程度明显减轻,心肌组织胶原沉积减少,心肌胶原纤维面积占比降低(P<0.01);心肌组织中CollagenⅠ、CollagenⅢ、CTGF mRNA表达水平均降低(P<0.05),IL-1β、IL-18表达水平降低(P<0.01,P<0.05)。结论:电针能够改善MIRI大鼠心室结构和功能,其机制可能与下调心肌炎性反应,抑制MIRI后心肌组织中CollagenⅠ、CollagenⅢ、CTGF mRNA的表达水平有关。

关键词：心肌缺血再灌注损伤电针心室重塑心肌纤维化炎性反应

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广州市2022年臭氧污染特征与成因分析

中国环境科学 2024年第6期44卷 3100-3110页

作者：黄继章高健余美芳高锐裴成磊杨素霞张金谱宋伟李磊李银松郭昊叶子铭广州市环境保护科学研究院有限公司广东广州510620 中国环境科学研究院北京100012 广东省广州生态环境监测中心站广东广州510006 中国科学院广州地球化学研究所广东广州510630 中山大学大气学院广东珠海519082 广州禾信仪器股份有限公司广东广州510535

选取2022年O_(3)及其前体物观测数据、气象观测数据和ERA5再分析数据,结合基于观测的模型(OBM)和后向轨迹模型,分析了2022年广州市O_(3)污染特征,识别了O_(3)生成控制区及关键敏感型物种,并从O_(3)前体物排放、气象条件和区域传输3个方... 详细信息

选取2022年O_(3)及其前体物观测数据、气象观测数据和ERA5再分析数据,结合基于观测的模型(OBM)和后向轨迹模型,分析了2022年广州市O_(3)污染特征,识别了O_(3)生成控制区及关键敏感型物种,并从O_(3)前体物排放、气象条件和区域传输3个方面开展了O_(3)污染成因分析.结果表明:广州市O_(3)生成以VOCs控制和NO_(x)-VOCs协同控制为主,现阶段VOCs和NO_(x)减排比例应不低于1:1;O_(3)生成对含氧VOCs最为敏感,其次是植物源VOCs(BVOCs)、烯烃、芳香烃和烷烃;2022年广州市O_(3)前体物NO_(x)和VOCs浓度同比下降14.7%和30.4%,但副高强盛、台风外围环流影响频繁叠加强辐射、高温、低湿等不利气象条件和区域传输抵消了广州市本地前体物减排的成效,造成O_(3)浓度上升,其中典型O_(3)污染过程(2022年9月16~22日)气流轨迹由西北、西南方向气团转变为东和东南方向气团,易受到来自周边城市区域传输影响.建议下一步持续推动NO_(x)减排,促进O_(3)对NO_(x)敏感性向正敏感性的转变,同时积极构建区域联防联控机制,降低区域传输贡献.

关键词：广州市臭氧污染基于观测的模型(OBM) 前体物气象条件区域传输

A new imaging mode based on X-ray CT as prior image and sparsely sampled projections for rapid clinical proton CT

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Nuclear Science and Techniques 2023年第8期34卷 64-74页

作者： Yu-Qing Yang Wen-Cheng Fang xiao-xia huang Qiang Du ming Li Jian Zheng Zhen-Tang Zhao Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China Shanghai Key Laboratory of Cryogenics and Superconducting RF Technology ShanghaiChina Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences SuzhouChina

Proton computed tomography(CT)has a distinct practical significance in clinical *** eliminates 3–5%errors caused by the transformation of Hounsfield unit(HU)to relative stopping power(RSP)values when using X-ray CT for positioning and treatment planning systems(TPSs).Following the development of FLASH proton therapy,there are increased requirements for accurate and rapid positioning in ***,a new rapid proton CT imaging mode is proposed based on sparsely sampled *** proton beam was boosted to 350 MeV by a compact proton linear accelerator(LINAC).In this study,the comparisons of the proton scattering with the energy of 350 MeV and 230 MeV are conducted based on GEANT4 *** the sparsely sampled information associated with beam acquisitions at 12 angles is not enough for reconstruction,X-ray CT is used as a prior *** RSP map generated by converting the X-ray CT was constructed based on Monte Carlo *** the estimation of the most likely path(MLP),the prior image-constrained compressed sensing(PICCS)algorithm is used to reconstruct images from two different phantoms using sparse proton projections of 350 MeV parallel proton *** results show that it is feasible to realize the proton image reconstruction with the rapid proton CT imaging proposed in this *** can produce RSP maps with much higher accuracy for TPSs and fast positioning to achieve ultra-fast imaging for real-time image-guided radiotherapy(IGRT)in clinical proton therapy applications.

关键词： Proton CT Real-time image guidance Image reconstruction Proton therapy

Mobilization of Inorganic Phosphorus from Soils by Ectomycorrhizal Fungi

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Pedosphere 2014年第5期24卷 683-689页

作者： ZHANG Liang WANG ming-xia LI Hua YUAN Ling huang Jian-Guo C.PENFOLD College of Resources and Environment Southwest University School of Biotechnology and Chemical Engineering Taizhou College Nanjing Normal University School of Agriculture Food and Wine The University of Adelaide

Ectomycorrhizal(EM) fungi could form symbiosis with plant roots and participate in nutrient absorption; however, many EM species commonly found in forest soils, where phosphorus(P) concentration and availability are usually very low, particularly in tropical and subtropical areas, have not yet been investigated for their efficiencies to mobilize soil P. In this study, fungal growth, P absorption,efflux of protons and organic acids, and soil P depletion by four isolates of EM fungi isolated either from acidic or calcareous soils were compared in pure liquid culture using soil as a sole P source. Boletus sp. 7(Bo 7), Lactarius deliciosus 3(Ld 3), and Pisolithus tinctorius 715(Pt 715) from acidic and P-deficient soils of southwestern China showed higher biomass and P concentration and accumulation than Cenococcum geophilum 4(Cg 4) from a calcareous soil of Inner Mongolia, northern China, after 4 weeks of liquid culture. Oxalate, malate, succinate, acetate, and citrate concentrations in the culture solutions varied significantly with fungal species,and oxalate accounted for 51.5%–91.4% of the total organic acids. Organic acids, particularly oxalate, in the culture solutions may lead to the solubilization of iron-bound P(Fe-P), aluminum-bound P(Al-P), and occluded P(O-P) from soil phosphates. Fungal species also varied greatly in proton efflux, which decreased the culture solution pH and may dissolve calcium-bound P(Ca-P) in *** could be the reason for the increment of both inorganic P in the culture solutions and Olsen P in the soil when EM fungi were present. Total inorganic P, the sum of Al-P, Fe-P, O-P, and Ca-P, in the culture solutions was positively correlated with the total concentration of organic acids in the culture solutions(r = 0.918*, n = 5), but negatively with both the total inorganic P in soil(r =-0.970**, n = 5) and the culture solution pH(r =-0.830*, n = 5). These suggested variable efficiencies of EM fungal species to mobilize inorganic P fracti

关键词： EM fungal species inorganic P organic acid P availability proton soil phosphate

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三叉神经痛诊疗的五项基本原则

中华疼痛学杂志 2024年第1期20卷 1-3页

作者：黄冰严敏夏令杰俞文华姚明浙江大学医学院附属第二医院疼痛科杭州市310009 河南省人民医院疼痛科郑州市450026 西湖大学医学院附属杭州市第一人民医院神经外科杭州市310006 嘉兴大学附属医院(嘉兴市第一医院)疼痛科浙江省嘉兴市314000

三叉神经痛是常见的头面痛疾病,也是疼痛科医生经常要面对的神经病理性疼痛之一。在三叉神经痛诊疗过程中应遵循"诊断与鉴别诊断优先、对因治疗优先的序贯性治疗、射频治疗时颅外射频优先、治疗选择个体化和确保患者知情同意"五项原则,... 详细信息

三叉神经痛是常见的头面痛疾病,也是疼痛科医生经常要面对的神经病理性疼痛之一。在三叉神经痛诊疗过程中应遵循"诊断与鉴别诊断优先、对因治疗优先的序贯性治疗、射频治疗时颅外射频优先、治疗选择个体化和确保患者知情同意"五项原则,以实现满意的治疗结果。

关键词：三叉神经痛诊断治疗原则

Global Pressure of One-Dimensional Polydisperse Granular Gases Driven by Gaussian White Noise

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Communications in Theoretical Physics 2007年第3X期48卷 481-486页

作者： CHEN Zhi-Yuan ZHANG Duan-ming YANG Feng-xia huang ming-Tao LI Rui ZHANG Ling ZHU Hong-Ying Department of Physics Huazhong University of Science and Technology Wuhan 430074 China Department of Physics Xianning College Xianning 437005 China

We study the global pressure of a one-dimensional polydisperse granular gases system for the first time, in which the size distribution of particles has the fractal characteristic and the inhomogeneity is described by a fractal dimension D. The particles are driven by Gaussian white noise and subject to inelastic mutual collisions. We define the global pressure P of the system as the impulse transferred across a surface in a unit of time, which has two contributions, one from the translational motion of particles and the other from the collisions. Explicit expression for the global pressure in the steady state is derived. By molecular dynamics simulations, we investigate how the inelasticity of collisions and the inhomogeneity of the particles influence the global pressure. The simulation results indicate that the restitution coefficient e and the fractal dimension D have significant effect on the pressure.

关键词： global pressure restitution coefficient e fractal dimension D Gaussian white noise polydisperse granular gases

Effect of temperature on mudstone disintegration process revealed with image analysis

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Journal of Mountain Science 2022年第7期19卷 2126-2135页

作者： YAN Luo-bin Srdjan PETROVIC huang Cheng XIE Chun-xia ZONG Hui-ming Milica KASANIN-GRUBIN School of Geographical Sciences Southwest UniversityChongqing400715China Danxia Landscape Research Institute Shaoguan512330China Institute of Chemistry Technology and MetallurgyDepartment of Catalysis and Chemical Engineering and Center for Environmental Chemistry and EngineeringUniversity of BelgradeBelgrade11000Serbia School of Geographical and Planning Sun Yat-sen UniversityGuangzhou410275China

Quantifying rock weathering processes,especially in ways of nondisturbance and on-site investigation,is one of the most critical tasks in predicting rocks erosion rates and understanding the sediment *** proposed a more reproducible approach to test how image analysis can quantify the changes in the size and shape of fragments during the weathering *** artificial models were designed to select suitable metrics among over 20 *** validate the efficiency of image analysis,we analyzed rocks from badlands in Nanxiong Basin,Southeast China,under three different ranges of temperature differences(TD)during cyclic wetting and drying(WD).Our results show that TDs can accelerate the disintegration rate,and even if there is only a 20℃ difference in the range of TDs,an apparent difference in fragment size was ***,the shape of fragments became more round as the increasing number of cyclic treatments,and for samples that went through the same number of treatments,the larger the temperature difference,the more round the shape *** that serves as another evidence for landscape evolution response to climatic warming.

关键词： Mechanical weathering Temperature effect Quantifying weathering rates Fragment size Fragment shape