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Comparison of fish communities using environmental DNA metabarcoding and capture methods in a plateau Erhai Lake,China

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Journal of Oceanology and Limnology 2024年第5期42卷 1597-1608页

作者： Hong CHEN wanchao HE Fenge YANG Li LIAO Chengjie yin Yushun CHEN Longgen GUO State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China Dali Monitoring Station of Yunnan Ecology and Environment Bureau Dali 671000China

Environmental DNA(eDNA)has been used as an important tool for fish diversity analysis,which can greatly solve the problems in traditional survey ***,little work has been done on the actual monitoring accuracy of *** this study,we analyzed the current status of fish resources in Erhai Lake in Yunnan,SW China,by dividing the lake into three sectors according to habitat differences,and compared the results of eDNA and traditional capture methods to investigate the shortcomings of the current analysis of eDNA results.A total of 27 fish species were detected by eDNA and traditional capture methods,including 20 and 19 fish species,respectively,and additional differences in fish composition between the two *** alpha diversity showed higher fish abundance and lower fish diversity by eDNA method compared to the traditional capture method,demonstrating that eDNA was not superior for use in fish diversity *** community similarity analysis showed that community differences were generally significant for eDNA(P<0.05).RDA analysis indicated that environmental factors did not significantly affect fish communities monitored by the eDNA ***,water temperature,aquatic plants,and water depth had significant(P<0.05)effects on fish communities in the traditional capture method,suggesting that eDNA results are insensitive to the effects of environmental *** results illustrate the effectiveness of eDNA in fish identification and the issues in quantification compared to traditional capture ***,combining eDNA with traditional methods is a more effective method for analyzing eDNA metabarcoding,following which the protocols of both quantitative methods can be designed to explore the regularity of eDNA quantification.

关键词： environmental DNA traditional capture method fish communities habitat differences Erhai Lake

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Catechol-Formaldehyde Resin Coated CdS Core-Shell Composite as Robust Photocatalyst for Long-Term Sustainable Artificial Photosynthesis of H_(2)O_(2)

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Chinese Journal of Chemistry 2024年第17期42卷 1975-1985页

作者： Yuexin Xiang Zhinan Xia wanchao Hu Cuiyan Tong Yang Xiao Changli Lu Institute of Chemistry Northeast Normal UniversityChangchunJilin130024China

Catechol-formaldehyde resin(CFR)is very attractive for H_(2)O_(2) production via the catalytic ***,the H2O2 formation is accompanied by the oxidation of catechol groups to o-benzoquinone groups on CFR,which will cause irreversible damage to CFR and greatly limit its long-term stable catalytic ***,CdS/CFR composite photocatalyst with a core-shell structure was synthesized by hydrothermal *** photogenerated electrons of Cds are used as a powerful driving force for the reversible redox conversion between catechol groups and o-benzoquinone groups on the CFR.

关键词： Hydrogen peroxide Photocatalysis Catechol-formaldehyde resin Cds Core-shell structures Oxygen reduction reaction Charge transfer Heterogeneous catalysis

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Exploring the taste presentation and receptor perception mechanism of salty peptides from Stropharia rugosoannulata based on molecular dynamics and thermodynamics simulation

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Food Science and Human Wellness 2024年第4期13卷 2277-2288页

作者： Wen Li Shuai Sun wanchao Chen Haile Ma Tingzhao Li Zhong Zhang Di Wu Mengqiu Yan Yan Yang Institute of Edible Fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of Agriculture and Rural AffairsShanghai 201403China School of Food&Biological Engineering Institute of Food Physical ProcessingJiangsu UniversityZhenjiang 212013China Amway(Shanghai)Innovation&Science Co. Ltd.Shanghai 201203China Amway(China)Botanical R&D Center Wuxi 214115China

The taste presentation and receptor perception mechanism of the salty peptide of Stropharia rugosoannulata were predicted and verified using peptide omics and molecular interaction *** combination of aspartic acid(D)and glutamic acid(E),or peptide fragments composed of arginine(R),constitute the characteristic taste structural basis of salty peptides of *** taste intensity of the salty peptide positively correlates with its concentration within a specific concentration range(0.25–1.0 mg/mL).The receptor more easily recognizes the first amino acid residue at the N-terminal of salty peptides and the aspartic acid residue in the ***513,ASP707,and VAL508 are the critical amino acid residues for the receptor to recognize salty ***1 is specifically the receptor for recognizing salty *** bonds and electrostatic interactions are the main driving forces for the interactions between salty peptides and TRPV1 *** has the most potent binding capacity with the receptor and has tremendous potential for application in sodium salt *** study confirmed the taste receptor that specifically recognizes salty peptides,analyzed the receptor-peptide binding interaction,and provided a new idea for understanding the taste receptor perception of salty peptides.

关键词： Salty peptide Molecular docking Biolayer interferometry Isothermal titration calorimetry

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Antiviral drug design based on structural insights into the N-terminal domain and C-terminal domain of the SARS-CoV-2 nucleocapsid protein

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Science Bulletin 2022年第22期67卷 2327-2335页

作者： Xiaodong Luan Xinming Li Yufan Li Gengchen Su wanchao yin Yi Jiang Ning Xu Feng Wang Wang Cheng Ye Jin Leike Zhang H.Eric Xu Yi Xue Shuyang Zhang School of Medicine Tsinghua UniversityBeijing 100084China Department of Cardiology Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China Tsinghua-Peking Center for Life Sciences Tsinghua UniversityBeijing 100084China School of Life Science Tsinghua UniversityBeijing 100084China Beijing Advanced Innovation Center for Structural Biology Beijing 100084China The CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China WuxiBiortus Biosciences Co. Ltd.Jiangyin 214437China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China

Nucleocapsid(N) protein plays crucial roles in the life cycle of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2), including the formation of ribonucleoprotein(RNP) complex with the viral *** we reported the crystal structures of the N-terminal domain(NTD) and C-terminal domain(CTD) of the N protein and an NTD-RNA complex. Our structures reveal a unique tetramer organization of NTD and identify a distinct RNA binding mode in the NTD-RNA complex, which could contribute to the formation of the RNP complex. We also screened small molecule inhibitors of N-NTD and N-CTD and discovered that ceftriaxone sodium, an antibiotic, can block the binding of RNA to NTD and inhibit the formation of the RNP complex. These results together could facilitate the further research of antiviral drug design targeting N protein.

关键词： SARS-CoV-2 Nucleocapsid protein N-terminal domain Ceftriaxone sodium

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The CropGPT project:Call for a global,coordinated effort in precision design breeding driven by Al using biological big data

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Molecular Plant 2024年第2期17卷 215-218页

作者： wanchao Zhu Rui Han Xiaoyang Shang Tao Zhou Chengyong Liang Xiaomeng Qin Hong Chen Zaiwen Feng Hongwei Zhang Xingming Fan Weifu Li Lin Li National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region College of AgronomyNorthwest A&F UniversityYanglingShaanxi712100China College of Informatics Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China institute of Food Crops Sciences Yunnan Academy of Agricultural Sciences2238 Beijing RoadKunmingYunnan 650200China Hubei Hongshan Laboratory Wuhan 430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityWuhan 430070China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Since the beginning of humanity,people have been seeking practical solutions to meet their most basic need:*** advent of agriculture greatly increased the food supply and led to a rapid increase in the human *** food production via agriculture can be attributed to both improved crop management practices and crop breeding through ***(crop)breeding has been categorized into four major stages based on the techniques employed(Wallace et al.,2018).

关键词： breeding seeking Wallace

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Polyphenol-rich extract from Sanghuangporous vaninii against acetaminophen-induced liver injury by blocking ferroptosis via NRF2/GPX4 pathway

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Food Science and Human Wellness 2024年

作者： Peng Liu Yuyang Wang wanchao Chen Di Wu Zhong Zhang Zhengpeng Li Anqiang Zhang Wen Li Yan Yang Institute of Edible Fungi， Shanghai Academy of Agricultural Sciences， National Engineering Research Center of Edible Fungi， Key Laboratory of Edible Fungi Resources and Utilization (South)， Ministry of Agriculture College of Food Science & Technology， Shanghai Ocean University College of Food Science and Engineering， Zhejiang University of Technology

Acetaminophen (APAP) used as an antipyretic and analgesic agent can cause acute liver injury (AILI) under overdose. Sanghuangporous vaninii (S. vaninii) is a edible fungus with abundant metabolites exhibits excellent hepatoprotective activities， but the effect for AILI is not yet fully understood. In this study， the polyphenol-rich extract from S. vaninii (PSV) was prepared， with a total phenolic content of 75.72% and 34 compounds. The data of hepatoprotection indicated that PSV obviously alleviated the hepatocellular injury induced by APAP in vivo and in vitro. The protective mechanism of PSV against APAP-induced AILI might be attributed to the activating NRF2/GPX4 pathway. Based on network pharmacology analysis， the active components of PSV such as caffeic acid， osmundacetone and hispolone played a key role in hepatoprotection of PSV. Consequently， this study highlights the protection of PSV on AILI， which provides new insight into bioactivities of S. vaninii.

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论新质生产力生成的文化动因——构建新质生产力文化理论的基本框架

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山东大学学报（哲学社会科学版） 2024年第4期 25-34页

作者：郭万超北京市社会科学院传媒与舆情研究所北京文化创意产业研究中心北京100101

文化是新质生产力的题中应有要义,把文化纳入其理论建构是创立中国经济学范式的新机遇和新路径。当今世界,文化与经济、政治等一起成为衡量综合国力的重要标准,文化软实力决定了一个国家的发展走向,并成为生产力突破的深层动因。从历时... 详细信息

文化是新质生产力的题中应有要义,把文化纳入其理论建构是创立中国经济学范式的新机遇和新路径。当今世界,文化与经济、政治等一起成为衡量综合国力的重要标准,文化软实力决定了一个国家的发展走向,并成为生产力突破的深层动因。从历时性过程考察,文化构成了新质生产力生成的基础性动力支撑,主要表现在:文化作为人类创造性活动的结晶深度嵌入其他社会结构,构成了新质生产力生成的精神动因;文化创新与科技创新二者互动是近代文明演进的重要方式,也形成了催生新质生产力的核心动力;经济文化化和文化经济化成为新质生产力的重要生长点。从共时性形态分析新质生产力的本质和现实形态,可以发现:文化赋予了新质生产力新的内在规定性;文化经济新业态、新消费构成了新质生产力的重要内容;我国现行国民经济行业分类中包含文化新质生产力的诸多具体实践形式。从人本性尺度审视新质生产力生成的人文价值内核,可以得出结论:新质生产力本质是解放人、发展人、成就人的生产力,也是推动文化经济发展的生产力;新质生产力必须摒弃“见物不见人”、缺乏人文关怀的资本主义生产特性;以人民为中心、让人民群众物质生活和精神生活都富裕是我国新质生产力发展的根本原则。

关键词：新质生产力文化经济文化动因人文价值

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催化反应增强痕量肿瘤标志物电化学检测性能研究进展

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首都师范大学学报（自然科学版） 2024年第3期45卷 64-73页

作者：李万超马占芳首都师范大学化学系北京100048

肿瘤标志物的灵敏检测是癌症早期诊断、治疗策略的选择和预后监测的重要保障手段之一。肿瘤标志物在血清中含量低,通常以痕量水平存在,甚至极个别的肿瘤标志物在每毫升血清中仅为飞克或皮克量级,其质量浓度的细微变化对于肿瘤的发生、... 详细信息

肿瘤标志物的灵敏检测是癌症早期诊断、治疗策略的选择和预后监测的重要保障手段之一。肿瘤标志物在血清中含量低,通常以痕量水平存在,甚至极个别的肿瘤标志物在每毫升血清中仅为飞克或皮克量级,其质量浓度的细微变化对于肿瘤的发生、发展和治疗效果具有一定的指示作用。如何实现痕量水平下肿瘤标志物微小质量浓度变化的灵敏检测具有重要的临床意义,并受到了人们的广泛关注。电化学传感器因具有高灵敏度、快速响应、易于操作及仪器小型化等特点,所以电化学免疫传感器检测痕量水平下肿瘤标志物微小质量浓度变化的研究,受到了人们的普遍关注并取得了巨大进展。本文主要综述了催化反应增强肿瘤标志物电化学检测性能方面取得的研究进展,特别关注了生物酶、纳米酶的应用以及电化学金属催化剂的使用,同时对有待解决的问题进行了展望。

关键词：肿瘤标志物电化学传感器催化反应生物酶纳米酶电化学金属催化剂

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大球盖菇酶解液中鲜味肽的分离鉴定及其协同增鲜效果分析

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食品科学 2024年第1期45卷 65-74页

作者：陈荣荣李文吴迪张忠鲍大鹏陈万超张劲松杨焱上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海201403 上海海洋大学食品学院上海201200 上海百信生物科技有限公司上海201403

利用超滤、凝胶过滤色谱结合感官评价和电子舌分析技术,分离大球盖菇酶解液中鲜味肽组分,对鲜味最强组分F3中的鲜味肽进行反向高效液相色谱-串联质谱鉴定,并将鉴定得到的鲜味肽合成后进一步探讨其呈味特性。结果表明,大球盖菇酶解液F3... 详细信息

利用超滤、凝胶过滤色谱结合感官评价和电子舌分析技术,分离大球盖菇酶解液中鲜味肽组分,对鲜味最强组分F3中的鲜味肽进行反向高效液相色谱-串联质谱鉴定,并将鉴定得到的鲜味肽合成后进一步探讨其呈味特性。结果表明,大球盖菇酶解液F3组分中鉴定得到8条鲜味肽段,分别为ELWR、RLVDR、KPDNR、AHLRF、LDWDR、LAEWR、DDWWR和EGHKGW,它们均具有鲜味特征和鲜味增强作用,阈值分别为0.30~1.33mmol/L和0.53~2.43 mmol/L;除LDWDR外,7条多肽对味精溶液均有不同程度的鲜味提升,提升幅度0.18%~61.12%,ELWR、RLVDR、KPDNR、AHLRF和EGHKGW 5条多肽在低浓度时表现出良好的鲜味提升效果。RLVDR和KPDNR协同增鲜的鲜味峰值质量浓度为5mg/mL,LDWDR、LAEWR、DDWWR和EGHKGW在4mg/mL时达到鲜味峰值。鲜味肽的氨基酸组成和空间结构会影响其味觉属性。本研究结果为大球盖菇鲜味肽的开发利用提供了理论依据。

关键词：大球盖菇酶解液鲜味肽分离鉴定协同增鲜效果

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香菇菌糠提取物处理对双孢蘑菇呼吸代谢的影响

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菌物学报 2024年第2期43卷 126-138页

作者：刘会琴李景军吴迪李文陈万超杨焱张忠安徽科技学院食品工程学院安徽滁州239000 上海市农业科学院食用菌研究所、农业农村部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心上海201403

为了延长双孢蘑菇Agaricus bisporus的保质期,本研究通过优化香菇Lentinula edodes菌糠提取物(LR-UE)采前喷施条件,探究其对双孢蘑菇贮藏过程中呼吸代谢的影响。研究表明,采前喷施的最佳条件是LR-UE的浓度为1.0mg/mL,喷施时间为采前1d,... 详细信息

为了延长双孢蘑菇Agaricus bisporus的保质期,本研究通过优化香菇Lentinula edodes菌糠提取物(LR-UE)采前喷施条件,探究其对双孢蘑菇贮藏过程中呼吸代谢的影响。研究表明,采前喷施的最佳条件是LR-UE的浓度为1.0mg/mL,喷施时间为采前1d,喷施量为200mL/m^(2)。LR-UE处理能够有效降低双孢蘑菇的失重,延缓双孢蘑菇呼吸峰的出现;同时,它还能调节双孢蘑菇糖酵解途径、三羧酸循环和磷酸戊糖途径的酶活性,减少丙二醛的积累,减少电解质的泄漏,维持较高的可溶性蛋白质含量,有效保持双孢蘑菇的贮藏品质。

关键词：双孢蘑菇香菇菌糠提取物采前喷施呼吸代谢

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