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Journal of Ocean University of China 2021年第6期20卷 1515-1527页

作者： ZHANG Meng li wanbo ZHU Yangyang WANG Weijia WANG Zhiyong Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture and Rural AffairsJimei UniversityXiamen 361021China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture Jimei UniversityXiamen 361021China

Elovl4 is a fatty acyl elongase which participates in the biosynthesis of long-chain polyunsaturated fatty acids(LCPUFA).Herein we isolated and functionally characterized the elovl4a gene in the large yellow croaker Larimichthys crocea,and investigated the regulatory effects of transcription factors Hnf4α,Lxrα,Pparαon the activity of elovl4a *** expression pattern revealed that elovl4a was widely expressed in several tissues and predominantly in *** expression in yeast showed that *** Elovl4a could effectively elongate both C18 and C20 PUFA substrates to C22 fatty *** addition,the affinity of large yellow croaker Elovl4a to n-6 series fatty acids is weak and cannot prolong 18:2n-6 and 18:***,*** elovl4a reporter activities were elevated by 1.31-,1.39-and 1.48-fold via over-expression of Lxrα,Pparαand Hnf4α,*** above findings can enrich the knowledge of biosynthesis pathway of LC-PUFA in the large yellow croaker and contribute to elucidate the LC-PUFA anabolism mechanism in fish.

关键词： Larimichthys crocea elovl4a long-chain polyunsaturated fatty acids promoter

Low-frequency blue energy harvesting for sustainable and active anticorrosion

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Nano Research 2023年第9期16卷 11871-11877页

作者： Miaomiao Cui Yawei Feng Hao Wu Yuankai Jin wanbo li Zuankai Wang Department of Mechanical Engineering City University of Hong KongHong KongChina Department of Mechanical Engineering The Hong Kong Polytechnic UniversityHong KongChina School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China Interdisciplinary Research Center for Engineering Science School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Engineering materials serving in marine surroundings are inevitably *** corrosive marine conditions can also be utilized to harvest kinetic ocean wave energy to solve this *** water–solid triboelectrification to harvest lowfrequency wave energy for active anticorrosion is *** techniques are efficient in harnessing environmental energy with frequencies higher than 3 Hz,whereas the dominated ocean waves with optimal wave spectral density fluctuate from 0.45 to 1.5 ***,we proposed a highly efficient and sustainable blue energy-powered cathodic protection(BECP)strategy by fusing water–solid triboelectric nanogenerators and cathodic protection *** the highly efficient triboelectrification between the moving water and hydrophobic fluorinated ethylene propylene tube,we developed the built-in power module,enabling the harvest of ocean wave energy lower than 1.5 *** generated volumetric current density is 28.9 mA·m^(-3),5–20 times higher than the values of the reported ***,the proposed BECP performs comparably to conventional cathodic protection in corrosion ***,the proposed approach can be easily applied to ships,buoys,and other offshore platforms to simultaneously realize blue energy harvesting and engineering material protection,providing an alternative to traditional active protection technology.

关键词： corrosion inhibition blue energy harvesting triboelectric nanogenerator cathodic protection

Interfacial friction at action: Interactions, regulation, and applications

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Friction 2023年第12期11卷 2153-2180页

作者： Zhiran YI Xiong WANG wanbo li Xuezhi QIN Yang li Kaiqiang WANG Yunting GUO Xing li Wenming ZHANG Zuankai WANG Department of Mechanical Engineering City University of Hong KongHong Kong 999077China Hong Kong Centre for Cerebro–caradiovasular Health Engineering(COCHE) Hong Kong 999077China State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing 100084China Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun 130022China

Friction is a fundamental force that impacts almost all interface-related *** the past decade,there is a revival in our basic understanding and practical applications of the *** this review,we discuss the recent progress on solid–liquid interfacial friction from the perspective of *** first discuss the fundamentals and theoretical evolution of solid–liquid interfacial friction based on both bulk interactions and molecular ***,we summarize the interfacial friction regulation strategies manifested in both natural surfaces and artificial systems,focusing on how liquid,solid,gas,and hydrodynamic coupling actions mediate interfacial ***,we discuss some practical applications that are inhibited or reinforced by interfacial *** last,we present the challenges to further understand and regulate interfacial friction.

关键词： interfacial friction solid-liquid interface interfacial interaction friction regulation

Mapping and analysis of a spatiotemporal H3K27ac and gene expression spectrum in pigs

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Science China(life Sciences) 2022年第8期65卷 1517-1534页

作者： Yaling Zhu Zhimin Zhou Tao Huang Zhen Zhang wanbo li Ziqi ling Tao Jiang Jiawen Yang Siyu Yang Yanyuan Xiao Carole Charlier Michel Georges Bin Yang Lusheng Huang State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural UniversityNanchang 330045China Laboratory Animal Research Center School of Basic Medical SciencesAnhui Medical UniversityHefei 230032China Unit of Animal Genomics GIGA-Institute and Faculty of Veterinary MedicineUniversity of Liege4000 LiegeBelgium

The limited knowledge of genomic noncoding and regulatory regions has restricted our ability to decipher the genetic mechanisms underlying complex traits in pigs. In this study, we characterized the spatiotemporal landscape of putative enhancers and promoters and their target genes by combining H3K27ac-targeted Ch IP-Seq and RNA-Seq in fetal(prenatal days 74–75) and adult(postnatal days 132–150) tissues(brain, liver, heart, muscle and small intestine) sampled from Asian aboriginal Bama Xiang and European highly selected Large White pigs of both sexes. We identified 101,290 H3K27ac peaks, marking 18,521promoters and 82,769 enhancers, including peaks that were active across all tissues and developmental stages(which could indicate safe harbor locus for exogenous gene insertion) and tissue-and developmental stage-specific peaks(which regulate gene pathways matching tissue-and developmental stage-specific physiological functions). We found that H3K27ac and DNA methylation in the promoter region of the XIST gene may be involved in X chromosome inactivation and demonstrated the utility of the present resource for revealing the regulatory patterns of known causal genes and prioritizing candidate causal variants for complex traits in pigs. In addition, we identified an average of 1,124 super-enhancers per sample and found that they were more likely to show tissue-specific activity than ordinary peaks. We have developed a web browser to improve the accessibility of the results(http://***/pencode/?genome=sus Scr11).

关键词： H3K27ac ChIP-Seq RNA-Seq pig XIST complex traits

All-perfluoropolymer,nonlinear stability-assisted monolithic surface combines topology-specific superwettability with ultradurability

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The Innovation 2023年第2期4卷 60-66,59页

作者： wanbo li Chiu-wing Chan Zeyu li Sin-Yung Siu Siyu Chen Han Sun Zeyu liu Yisu Wang Chong Hu Nicola Maria Pugno Richard N.Zare Hongkai Wu Kangning Ren Department of Chemistry Hong Kong Baptist UniversityHong Kong 999077China Department of Chemistry The Hong Kong University of Science and TechnologyHong Kong 999077China Department of Civil Environmental and Mechanical EngineeringLaboratory of Bio-InspiredBionicNanoMeta Materials and MechanicsUniversitàdi Trento38100 TrentoItaly School of Engineering and Materials Science Queen Mary University of LondonLondon E14NSUK Department of Chemistry Stanford UniversityStanfordCA 94305USA School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Environmental and Biological Analysis Hong Kong Baptist UniversityHong Kong SAR 999077China HKBU Institute of Research and Continuing Education Shenzhen 518057China

Developing versatile and robust surfaces that mimic the skins of living beings to regulate air/liquid/solid matter is critical for many bioinspired *** notable achievements,such as in the case of developing robust superhydrophobic surfaces,it remains elusive to realize simultaneously topology-specific superwettability and multipronged durability owing to their inherent tradeoff and the lack of a scalable fabrication ***,we present a largely unexplored strategy of preparing an all-perfluoropolymer(Teflon),nonlinear stability-assisted monolithic surface for efficient regulating matters.

关键词： method durability topology

沸腾态气-液-固搅拌槽内宏观混合时间的研究

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北京化工大学学报（自然科学版） 2014年第3期41卷 20-23页

作者：栗万博覃懿达高正明北京化工大学化学工程学院北京100029

在直径为0.476 m的椭圆底搅拌槽内,采用电导率法研究了沸腾态气-液-固三相体系内混合时间特性。主要考察分散相(气体、颗粒)和功耗对混合时间的影响。实验结果表明:沸腾态搅拌槽内,同转速条件下,颗粒体积分数对单位质量功大小影响较小;... 详细信息

在直径为0.476 m的椭圆底搅拌槽内,采用电导率法研究了沸腾态气-液-固三相体系内混合时间特性。主要考察分散相(气体、颗粒)和功耗对混合时间的影响。实验结果表明:沸腾态搅拌槽内,同转速条件下,颗粒体积分数对单位质量功大小影响较小;仅转速高于480 r/min范围内,表观气速增加,体系单位质量功略有下降。颗粒临界悬浮转速随颗粒体积分数的增加而增加,但不随表观气速的变化而发生变化。沸腾态气-液-固三相体系内,混合时间随表观气速或颗粒体积分数的升高而延长。

关键词：气-液-固搅拌槽宏观混合混合时间

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大黄鱼性别特异SNP标记的开发与验证

水产学报 2018年第9期42卷 1329-1337页

作者：林晓煜肖世俊李完波王志勇集美大学水产学院农业部东海海水健康养殖重点实验室

大黄鱼是我国养殖量最大的海水经济鱼类,其雌鱼生长显著快于雄鱼,但两性的外部形态差异不明显,也没有异形性染色体,依靠传统方法无法对其活体准确进行生理性别和遗传性别的判别与鉴定,需要开发性别特异的分子标记。本研究从2尾雌鱼和2... 详细信息

大黄鱼是我国养殖量最大的海水经济鱼类,其雌鱼生长显著快于雄鱼,但两性的外部形态差异不明显,也没有异形性染色体,依靠传统方法无法对其活体准确进行生理性别和遗传性别的判别与鉴定,需要开发性别特异的分子标记。本研究从2尾雌鱼和2尾雄鱼、以及分别由50雌鱼与50尾雄鱼组成的2个混合样品的基因组重测序数据比较中筛选与性别显著关联的SNP位点,对其中11个位点分别设计引物在15尾雌鱼和15尾雄鱼中扩增出PCR产物进行Sanger测序验证,鉴定出1个与性别完全连锁的位点(SNP6,15尾雌鱼均为纯合、15尾雄鱼均为杂合)。然后,设计等位基因特异性PCR引物,其中包括2条雌性与雄性通用引物和1条雄性特异引物,在闽—粤东族与岱衢族大黄鱼合计近2 200个个体中进行扩增,结果在全部雌鱼中都只扩增出1个348 bp的条带,而在全部雄鱼中还扩增出1个194 bp的Y染色体特异条带,检出率达到100%。研究表明,大黄鱼属于XX♀-XY♂类型的性别决定。本研究鉴定出一个雄性特异SNP标记,并建立了一种新的大黄鱼遗传性别鉴定技术,为大黄鱼单性育种、基因组选择育种和性别决定分子机制研究提供了重要的技术手段。

关键词：大黄鱼性别特异SNP 遗传性别鉴定 AS-PCR

大黄鱼雌雄性腺长链非编码RNA的挖掘与差异分析

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中国水产科学 2019年第5期26卷 852-860页

作者：王伟佳韩兆方李完波王志勇集美大学农业农村部东海海水健康养殖重点实验室青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室

为探究长链非编码RNA在大黄鱼性腺发育与分化中的作用,从雌雄各3尾大黄鱼(Larimichthys crocea)的性腺中提取总RNA,进行去除rRNA的链特异性转录组建库和二代测序。将测序数据比对到大黄鱼参考基因组,经比对、组装共得到来自31675个基因... 详细信息

为探究长链非编码RNA在大黄鱼性腺发育与分化中的作用,从雌雄各3尾大黄鱼(Larimichthys crocea)的性腺中提取总RNA,进行去除rRNA的链特异性转录组建库和二代测序。将测序数据比对到大黄鱼参考基因组,经比对、组装共得到来自31675个基因的66088个转录本,严格筛选得到来自3984个基因位点的5162条lncRNA。进一步分析获得了在大黄鱼雌雄性腺中差异表达的mRNA9341个,lncRNA2782个,高度相关的lncRNA-mRNA对1227个;有多个lncRNA靶向已知的性别分化和发育相关基因,其中lncRNA MSTRG.24346与大黄鱼的性别决定候选基因dmrt1距离相近,且相关性极显著。该研究表明lncRNA可能在大黄鱼性别分化中起到重要作用,值得深入研究阐明机制。

关键词：大黄鱼性腺 lncRNA 性别分化

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TA17钛合金热变形行为及加工图

钢铁钒钛 2017年第2期38卷 46-52页

作者：任万波李军于辉王莹攀钢集团研究院有限公司钒钛资源综合利用国家重点实验室四川攀枝花617000 燕山大学机械工程学院河北秦皇岛066004

为探索TA17钛合金热变形行为和变形特性,采用Gleeble-3800热模拟机开展温度为700~1 100℃、应变速率为0.1~40 s^(-1)、变形程度为60%的热压缩试验。基于Arrhenius模型构建TA17钛合金的本构方程,基于动态材料模型构建TA17钛合金的热加工... 详细信息

为探索TA17钛合金热变形行为和变形特性,采用Gleeble-3800热模拟机开展温度为700~1 100℃、应变速率为0.1~40 s^(-1)、变形程度为60%的热压缩试验。基于Arrhenius模型构建TA17钛合金的本构方程,基于动态材料模型构建TA17钛合金的热加工图(ε=0.6),并结合显微组织分析对热加工图进行验证。结果表明:热加工图预测结果与组织分析相符,当温度低于750℃或者应变速率大于10 s^(-1)的区域为TA17钛合金的加工失稳区域,失稳区以外是安全加工区域,热加工性能最佳的区域是800℃、0.1 s^(-1)。

关键词： TA17钛合金本构方程热加工图失稳区显微组织

Pr_2O_3掺杂SnO_2陶瓷电极的制备及性能表征

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中国稀土学报 2010年第6期28卷 705-708页

作者：汪庆卫王宏志宁伟李万波东华大学材料科学与工程学院上海201620 先进玻璃制造技术教育部工程研究中心上海200052

通过共沉淀法制备了掺杂Pr2O3的二氧化锡电极样品,主要研究了在SnO2陶瓷电极中添加不同含量的Pr2O3对形貌结构及性能的影响。借助于XRD和SEM分析试样的晶型结构和微观形貌,结果表明Pr2O3能促进二氧化锡晶粒长大,并产生新的第二相Pr2Sn2O... 详细信息

通过共沉淀法制备了掺杂Pr2O3的二氧化锡电极样品,主要研究了在SnO2陶瓷电极中添加不同含量的Pr2O3对形貌结构及性能的影响。借助于XRD和SEM分析试样的晶型结构和微观形貌,结果表明Pr2O3能促进二氧化锡晶粒长大,并产生新的第二相Pr2Sn2O7。当Pr2O3含量从1%增加到5%(质量分数)时,第二相依次增多并镶嵌在主晶格中。通过电阻率和力学性能的测定,发现掺杂3%Pr2O3有利于提高SnO2电极的烧结致密化和降低电阻率,过量的Pr2O3则会产生过多第二相而不利于致密化烧结。

关键词：掺杂氧化镨二氧化锡表征稀土