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Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis

Infectious Diseases of Poverty 2018年第1期7卷 761-769页

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo zhong-Zi Lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams wan-zhong jia Donald P.McManus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAASLanzhou 730046People’s Republic of China The Human Pathology and Immunology Department Ningxia Medical UniversityYinchuanNingxia Hui Autonomous RegionPeople’s Republic of China Neurosurgery Department Tianjin Xiqing HospitalTianjinPeople’s Republic of China Molecular Parasitology Laboratory QIMR Berghofer Medical Research InstituteBrisbaneAustralia Research School of Population Health Australian National UniversityCanberraAustralia School of Population Health Infectious Disease Epidemiology UnitUniversity of QueenslandBrisbaneAustralia Queensland Alliance for Agriculture and Food Innovation University of QueenslandGattonAustralia

Background:Cystic echinococcosis(CE)and alveolar echinococcosis(AE)are highly endemic in Xiji County of Ningxia Hui Autonomous Region(NHAR)in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding *** study is to determine the current prevalence of Echinococcus granulosus and *** in domestic dogs and monitor the echinococcosis transmission ***:Study villages were selected using landscape patterns(Geographic Information System,GIS)for Echinococcus transmission“hot spots”,combined with hospital records identifying risk areas for AE and CE.A survey of 750 domestic dogs,including copro-sampling and owner questionnaires,from 25 selected villages,was undertaken in 2012.A copro-multiplex PCR assay was used for the specific diagnosis of *** and *** in the *** analysis,using IBM SPSS Statistics,was undertaken,to compare the prevalence of the two Echinococcus *** dogs between four geographical areas of Xiji by theχ^(2)*** analysis of the combinations of outcomes from the questionnaire and copro-PCR assay data was carried out to determine the significant risk factors for dog ***:The highest de-worming rate of 84.0%was found in the northwest area of Xiji County,and significant differences(P0.05)in the prevalence of *** in dogs from the northwest,southwest,northeast,and southeast of Xiji,but there were significant differences(P<0.05)between dogs infected with *** from the four *** of the other independent variables was statistically ***:The results from this study indicate a high prevalenc

关键词： Domestic dog surveys Dog-copro-multiplex PCR assay Co-endemicity of Echinococcus granulosus and E.multilocularis Xiji County Ningxia hui autonomous region(NHAR) P.R.China

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Changes in the canine intestinal microbiome in response to Echinococcus spp. at different time post-infection

Changes in the canine intestinal microbiome in response to E...

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第五届全国人畜共患病学术研讨会

作者： Hong-bin Yan Shuang-nan Li Li Li Wenhui Li Gabriel Rinaldi Nianzhang Zhang Yan-tao Wu Bao-quan Fu wan-zhong jia State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceKey Laboratory of Veterinary Public Health of Agriculture MinistryLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Parasite Genomics Wellcome Trust Sanger InstituteWellcome Trust Genome Campus

The gastrointestinal tract of mammals is a complex ecosystem with distinct environments and comprises hundreds of different types of bacterial *** gut microbiota may play a critical role in the gut health and interaction between the parasite and *** herein attempted to identify a microbiota shift that may be affected by the infection of Echinococcus granulosus and E.

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基于HYDRUS模型的盐碱地土壤水盐运移模拟

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干旱地区农业研究 2017年第1期35卷 135-142页

作者：潘延鑫罗纨贾忠华井思媛李山武迪扬州大学水利与能源动力工程学院江苏扬州225009 南昌工程学院水利与生态工程学院江西南昌330099 西安理工大学西北旱区生态水利工程国家重点实验室培育基地陕西西安710048

为了解陕西卤泊滩盐碱地的水盐运移情况,基于当地2009—2013年田间水盐监测资料,应用饱和-非饱和土壤水分及溶质运移理论,利用HYDRUS-1D数值模型对当地土壤水分、盐分运移规律进行数值模拟,分析了盐碱地的水盐变化状况,确定合理的田间... 详细信息

为了解陕西卤泊滩盐碱地的水盐运移情况,基于当地2009—2013年田间水盐监测资料,应用饱和-非饱和土壤水分及溶质运移理论,利用HYDRUS-1D数值模型对当地土壤水分、盐分运移规律进行数值模拟,分析了盐碱地的水盐变化状况,确定合理的田间灌水定额。结果表明:在玉米整个生育期内,不同灌溉处理的土壤含水量变化趋势基本一致,从节水控盐的综合标准衡量,农田灌水定额为500 m3·hm-2时有利于控制土壤盐分的累积。采用HYDRUS-1D模型对盐碱地农田土壤水盐运移的模拟结果与田间试验实测结果基本吻合,该研究结果可为类似盐碱化地区农田水盐管理提供科学依据。

关键词：盐碱地水盐运移数值模拟 HYDRUS-1D模型

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Identification and characterization of a cathepsin-F-like protease in Trichinella spiralis

Identification and characterization of a cathepsin-F-like pr...

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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Zi-gang Qu Xue-ting Ma Wen-hui Li Nian-zhang Zhang Long Yue jian-min Cui jian-ping Cai wan-zhong jia Bao-quan Fu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Public Health of the Ministry of AgricultureKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease

Trichinellosis is a an emerging and reemerging infectious disease,caused by Trichinella *** F belongs to cysteine protease that is a major virulence factor for parasitic helminths,and it may be a potential anti-helminth drug target and vaccine *** this study,the cDNA encoding a putative cathepsin F-like peptidase in Trichinella spiralis,TsCF1,was obtained and its biochemical characterization and expression profile were *** demonstrated that the TsCF1 gene encodes a protein of 366 aa with the theoretical molecular weight of 41.9 ku and isoelectric point of *** signal peptide sequence of TsCF1 located between amino acids 1 and 19 and 3 putative N-glycosylation sites were *** cysteine protease conserved active site of Cys173,His309 and Asn333 were identified and cathepsin F specific motif ERFNAQ like KLFNAQ sequence was revealed in the propeptide of *** analysis revealed a higher than 40%identity with other cathepsin F from parasitic helminth and phylogenetic analysis indicated TsCF1 located in the different clans with trematode cathepsin ***-PCR revealed the gene was expressed in muscle larvae,newborn larvae and adult *** SDS-PAGE revealed the recombinant protein was expressed with the molecular weight of 45 *** analysis showed that TsCF1 located on the inner layer of the cuticle and stichosome of the parasite,which probably play a vital role in nutrition *** purified recombinant TsCF1 was used to immunize rabbit and the immune serum could recognize a band of soluble protein of muscle larvae at about 46 *** renaturation the TsCF1 recombinat protein demonstrated substantial enzyme activity to Z-Phe-Arg-AMC substrate with the optimal pH 5.5 and this activity could be inhibited by cysteine protease inhibitor *** analysis revealed the kinetic parameters of the recombinant peptidase to be Km=0.5091 μM and Vmax= 6.12 RFU/sμM at pH 5.5,and the IC50 value of E64 was 1

关键词： Trichinella spiralis cysteine protease Cathepsin F enzyme activity

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TRIME-T3用于盐碱地土壤含水量测定的试验研究

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干旱区地理 2017年第1期40卷 108-113页

以陕西省富平县卤泊滩盐碱地改良区为例,在其上游地段选取两块农田为研究对象,布置典型观测断面并埋设测管,运用TRIME-T3法和烘干法对观测断面土壤剖面含水量进行测定,并同步测定土壤含盐量。对两种方法测得的土壤含水量进行对比分析,... 详细信息

以陕西省富平县卤泊滩盐碱地改良区为例,在其上游地段选取两块农田为研究对象,布置典型观测断面并埋设测管,运用TRIME-T3法和烘干法对观测断面土壤剖面含水量进行测定,并同步测定土壤含盐量。对两种方法测得的土壤含水量进行对比分析,研究土壤盐分对TRIME-T3法测定的土壤含水量影响以及该方法在盐碱地农田土壤剖面含水量测试中的应用。研究结果显示:(1)TRIME-T3法测量值较烘干法值偏大,但两者之间有较高的相关性,回归分析结果表明可以用y=0.866 3x+0.754 5的线性函数进行校正,校正后的TRIME-T3测量值中将有94.4%的值接近土壤含水量真实值。(2)通过对校正函数进行实例验证,结果表明TRIME-T3标定曲线值与烘干法测定值非常接近,绝对误差<1 cm3·cm-3,相对误差在5%以下,表明TRIME-T3法通过一定的校正后可以很好地表达该盐碱地农田土壤剖面含水量信息。(3)通过对TRIME-T3测定的含水量与相应含盐量进行分析,发现两者之间的相关性并不显著,表明该方法可适用于盐碱地农田土壤剖面含水量测定。该研究结果可为类似地区盐碱地土壤剖面水分测定技术(TRIME-T3)的应用提供科学依据和理论参考。

关键词： TDR 盐碱地土壤含水量电导率

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Prevalence of Echinococcus granulosus and *** in domestic dogs in a Chinese community highly endemic for echinococcosis

Prevalence of Echinococcus granulosus and *** in domestic do...

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第五届全国人畜共患病学术研讨会

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo zhong-Zi Lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams wan-zhong jia Donald P.McManus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAAS Neurosurgery Department Tianjin Xiqing Hospital Ningxia Medical Universities Molecular Parasitology Laboratory QIMR Berghofer Medical Research Institute Research School of Population Health Australian National University School of Population Health Infectious Disease Epidemiology UnitUniversity of Queensland Queensland Alliance for Agriculture and Food Innovation University of Queensland

Background:To undertake a survey to determine the infection prevalence of Echinococcus granulosus and *** in domestic dogs and to monitor the transmission dynamics of echinococcosis in Xiji County,Ningxia Hui Autonomous Region(NHAR),***,an area co-endemic for both human alveolar and cystic echinococcosis(AE and CE)where a control campaign,based on dog de-worming with praziquantel(PZQ),has been in operation over the past

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Infection of sheep by Echinococcus multilocularis in Gansu,China:evidence from mitochondrial and nuclear DNA analysis

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Infectious Diseases of Poverty 2023年第4期12卷 107-107页

作者： Nigus Abebe Shumuye Li Li Wen-Hui Li Nian-Zhang Zhang Yan-Tao Wu Yao-Dong Wu Wen-Jun Tian Lin-Sheng Zhang Xiao-Feng Nian Guo-Dong Dai Wei-Gang Chen Sheng-Zhi Gao Xue-Qi Tian Jun-Shi Liu Bin Li Nigatu Kebede Bao-Quan Fu Hong-Bin Yan wan-zhong jia State Key Laboratory for Animal Disease Control and Prevention College of Veterinary Medicine National Para-Reference Laboratory for Animal Echinococcosis Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Lanzhou University Chinese Academy of Agricultural Sciences Lanzhou China Department of Veterinary Clinical Medicine and Epidemiology Mekelle University College of Veterinary Sciences Kalamino Campus Mekelle Ethiopia Gansu Animal Centre for Disease Control and Prevention Lanzhou China Jingyuan County Animal Centre for Disease Control and Prevention Jingyuan County Animal Husbandry and Veterinary Technical Service Center Jingyuan China Aklilu Lemma Institute of Pathobiology Addis Ababa University Addis Ababa Ethiopia jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou China

BackgroundIn the normal life cycle of the parasite(Echinococcus multilocularis)that causes alveolar echinococcosis,domestic and wild carnivores act as definitive hosts,and rodents act as intermediate *** presented study contributes to the research on the distribution and transmission pattern of *** in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province,*** 2020 to 2021,nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination.A near complete mitochondrial(mt)genome and selected nuclear genes were amplified from the cyst-like lesion for *** confirm the status of the specimen,comparative analysis with reference sequences,phylogenetic analysis,and network analysis were *** isolates displayed≥98.87%similarity to *** NADH dehydrogenase sub-unit 1(nad1)(894 bp)reference sequences deposited in ***,amplification of the nad4 and nad2 genes also confirmed all nine samples as *** with>99.30%***,three nuclear genes,pepck(1545 bp),elp-exons VII and VIII(566 bp),and elp-exon IX(256 bp),were successfully amplified and sequenced for one of the isolates with 98.42%similarity,confirming the isolates were correctly identified as *** analysis also correctly placed the isolates with other *** a result of the discovery of *** in an unusual intermediate host,which is considered to have the highest zoonotic potential,the result clearly demonstrated the necessity for expanded surveillance in the area.

关键词： Echinococcus multilocularis China Sheep Liver Phylogeny

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Recent advances in phenothiazine-based dyes for dye-sensitized solar cells

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Chinese Chemical Letters 2016年第8期27卷 1304-1318页

作者： Jun-Sheng Luo zhong-Quan wan Chun-Yang jia State Key Laboratory of Electronic Thin Films and Integrated Devices School of Microelectronics and Solid-State ElectronicsUniversity of Electronic Science and Technology of China

Dye-sensitized solar cells（DSSCs） have attracted significant attention as alternatives to conventional silicon-based solar cells owing to their low-cost production,facile fabrication,excellent stability and high power conversion efficiency（PCE）.The dye molecule is one of the key components in DSSCs since it significant influence on the PCE,charge separation,light-harvesting,as well as the device *** various dyes,easily tunable phenothiazine-based dyes hold a large proportion and achieve impressive photovoltaic *** class of dyes not only has superiorly non-planar butterfly structure but also possesses excellent electron donating ability and large π conjugated *** review summarized recent developments in the phenothiazine dyes,including small molecule phenothiazine dyes,polymer phenothiazine dyes and phenothiazine dyes for co-sensitization,especially focused on the developments and design concepts of small molecule phenothiazine dyes,as well as the correlation between molecular structures and the photovoltaic performances.

关键词： Dye-sensitized solar cells Molecular engineering Small molecule phenothiazine dyes Polymer phenothiazine dyes Co-sensitization Power conversion efficiency

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A PENALTY FUNCTION METHOD FOR SOLVING ILL-POSED BILEVEL PROGRAMMING PROBLEM VIA WEIGHTED SUMMATION

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Journal of Systems Science & Complexity 2013年第6期26卷 1019-1027页

作者： jia Shihui wan zhongping Math Department School of ScienceWuhan University of Science and Technology Hubei Province Key Laboratory of Systems Science in Metallurgical Process Wuhan University of Science and Technology School of Mathematics and Statistics Wuhan University

For ill-posed bilevel programming problem,the optimistic solution is always the best decision for the upper level but it is not always the best choice for both levels if the authors consider the model's satisfactory degree in *** acquire a more satisfying solution than the optimistic one to realize the two levels' most profits,this paper considers both levels' satisfactory degree and constructs a minimization problem of the two objective functions by weighted ***,using the duality gap of the lower level as the penalty function,the authors transfer these two levels problem to a single one and propose a corresponding ***,the authors give an example to show a more satisfying solution than the optimistic solution can be achieved by this algorithm.

关键词： Bilevel programming duality gap penalty function satisfactory degree weighted sum-mation.

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Interactive effect of minerals on complex ore flotation: A brief review

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International Journal of Minerals,Metallurgy and Materials 2020年第5期27卷 571-583页

作者： wan-zhong Yin Yuan Tang School of Resources and Civil Engineering Northeastern UniversityShenyang 110819China

Froth flotation is the most effective industrial method used to separate fine-grained minerals. The main problem of complex ore flotation is the negative effect of interactions among minerals in slurry, leading to variation in surface properties during separation. In this review,studies on the interactive effect among minerals on the flotation of iron ores, magnesite ores, and scheelite ores are summarized, and the main problems and mechanisms that diminish the separation efficiency of minerals are revealed in detail. Recent research progress on the flotation of these ores has confirmed that mineral aggregation, coating, and dissolution, as well as other factors caused by interacting behavior, explain the depressing effects of fine particles on mineral separation. Solvable methods for these effects are further discussed. Novel flotation processes and more selective reagents are critical for further investigations on various approaches to improve the beneficiation efficiency of these *** review aims to provide a good reference for conducting studies related to complex ore flotation.

关键词： interactive effect flotation iron ores magnesite ores scheelite ores

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