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Unusual peroxidase activity of a myoglobin mutant with two distal histidines

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Chinese Chemical Letters 2012年第6期23卷 741-744页

作者： Wei Wei Guo Dun wan Li Fu Liao ying wu Lin School of Chemistry and Chemical Engineering University of South China Hengyang 421001 China State Key Laboratory of Coordination Chemistry Nanjing University Nanjing 210093 China

By retaining the native distal His64 in sperm whale myoglobin （Mb）, a second distal histidine was engineered in Mb by mutating Leu29 to His29. The resultant mutant of L29H Mb exhibits an unusual enhanced peroxidase activity with a positive cooperativity in comparison to that of wild type Mb. The new enzyme with two cooperative distal histidines has not been found in native peroxidase, which emohasizes a creation of the rational nmt^in doclan

关键词： Heme protein Myoglobin Protein design Peroxidase Cooperativity

Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3287-3301页

作者： Ziwei Fan Mengxian Jia Jian Zhou Zhoule Zhu Yumin wu Xiaowu Lin Yiming Qian Jiashu Lian Xin Hua Jianhong Dong Zheyu Fang Yuqing Liu Sibing Chen Xiumin Xue Juanqing Yue Minyu Zhu ying wang Zhihui Huang Honglin Teng Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Neurology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord *** of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be *** possesses an anti-inflammatory biological ***,its roles and the underlying molecular mechanisms in spinal cord injury remain *** this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in *** promoted M2 polarization through inactivating the cGAS/STING/NF-κB ***,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord ***,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal *** together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

关键词： cGAS/STING functional recovery microglia neuroinflammation neuroprotection nuclear factor-κB polarization spinal cord injury tryptanthrin

Combined apatite fission-track dating, chlorine and REE content analysis by LA-ICPMS

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Science Bulletin 2017年第22期62卷 1497-1500页

作者： Jianzhang Pang Dewen Zheng Yan Ma ying wang ying wu Jinglin wan Jingxing Yu Youjuan Li Yizhou wang State Key Laboratory of Earthquake Dynamics Institute of Geology China Earthquake Administration Beijing 100029 China Department of Earth Sciences University of Oxford Oxford OX1 3AN UK

As one of the best established low temperature thermochronology techniques,apatite fission-track(AFT)analysis has proved an important tool for constraining a wide variety of upper crustal processes,e.g.,landscape evolution,hydrocarbon exploration and tectonics[1].The most common method employed for AFT analysis is the external detector method(EDM)[1]and this involves the thermal neutron irradiation of samples for determination of

关键词： chlorine and REE content analysis by LA-ICPMS Cl REE Combined apatite fission-track dating

Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes mellitus:a cross-sectional cohort study

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Frontiers of Medicine 2020年第5期14卷 642-650页

作者： Huiwen Ren Can wu ying Shao Shuang Liu Yang Zhou Qiuyue wan Department of Endocrinology the First Hospital of China Medical UniversityShenyang 110001China Advanced Institute for Medical Sciences Dalian Medical UniversityDalian 116044China Department of Gastroenterology and Endoscopy the First Hospital of China Medical UniversityShenyang 110001China Department of Endocrinology the Second Hospital of China Medical UniversityShenyang 110001China Severe Infection Intensive Care Unit Affiliated Central Hospital of Shenyang Medical CollegeShenyang 110001China

This study aimed to investigate the correlation between serum miR-154-5p and urinary albumin to creatinine ratio(UACR)in patients with type 2 diabetes mellitus(T2DM)and the association with biomarkers of inflammation and fibrosis in diabetic kidney disease(DKD).A total of 390 patients with T2DM were divided into three groups:normal albuminuria(UACR300 mg/g,n=122,CA).Circulating miR-154-5p,inflammatory(C-reactive protein(CRP);erythrocyte sedimentation rate(ESR);and tumor necrosis factor-a(TNF-α)and fibrotic markers(vascular endothelial growth factor(VEGF);transforming growth factor-β1(TGF-β1);and fibronectin(FN),and other biochemical indicators were assessed via real-time PCR,enzyme-linked immunosorbent assay,and chemiluminescence assay in patients with T2DM and 138 control subjects(NC).UACR,miR-154-5p,glycated hemoglobin(HbA1c),serum creatinine(sCr),blood urea nitrogen(BUN),ESR,CRP,VEGF,TNF-α,TGF-β1,and FN were significantly higher and the estimated glomerular filtration rate(eGFR)was significantly lower in NA,MA,and CA groups than in NC subjects(P<0.05).Elevated levels of UACR and miR-154-5p were directly correlated with HbA1c,sCr,BUN,ESR,CRP,VEGF,TNF-α,TGF-β1,and FN and negatively correlated with eGFR(P<0.05).miR-154-5p,HbA1c,sCr,BUN,eGFR,ESR,CRP,VEGF,TNF-α,TGF-β1,and FN were important factors affecting *** findings indicated that elevated serum miR-154-5p is significantly correlated with high UACR in patients with T2DM and may offer a novel reference for the early diagnosis of DKD.

关键词： type 2 diabetes mellitus diabetic kidney disease miR-154-5p urinary albumin to creatinine ratio

States and transitions in mixed networks

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Frontiers of physics 2014年第4期9卷 523-528页

作者： ying Zhang Wen-Hui wan Department of Physics Beijing Normal University Beijing 100875 China Department of Physics Beijing Institute of Technology Beijing 100081 China

A network is named as mixed network if it is composed of N nodes, the dynamics of some nodes are periodic, while the others are chaotic. The mixed network with all-to-all coupling and its correspond- ing networks after the nonlinearity gap-condition pruning are investigated. Several synchronization states are demonstrated in both systems, and a first-order phase transition is proposed. The mixture of dynamics implies any kind of synchronous dynamics for the whole network, and the inixed networks may be controlled by the nonlinearity gap-condition pruning.

关键词： mixed network phase transition synchronization state

不同宿主来源产气荚膜梭菌生物被膜形成前后药物敏感性分析

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动物医学进展 2025年第1期46卷 38-42页

作者：吴莹邵长军刘文华青岛农业大学动物医学院山东青岛266109 烟台市农科院山东烟台264200

为了检测5株不同宿主来源产气荚膜梭菌形成生物被膜能力及其对抗菌药物的敏感性差异,首先用纸片扩散法药敏试验测定5株不同来源的产气荚膜梭菌对7种抗菌药物的敏感性;筛选高敏药物测定各菌株的最小抑菌浓度(MIC);利用结晶紫染色法检测... 详细信息

为了检测5株不同宿主来源产气荚膜梭菌形成生物被膜能力及其对抗菌药物的敏感性差异,首先用纸片扩散法药敏试验测定5株不同来源的产气荚膜梭菌对7种抗菌药物的敏感性;筛选高敏药物测定各菌株的最小抑菌浓度(MIC);利用结晶紫染色法检测不同来源产气荚膜梭菌生物被膜形成能力和高敏药物对生物被膜的抑制和清除能力。药敏结果显示,5株不同宿主来源产气荚膜梭菌均对氨苄西林高度敏感,对其余6种抗菌药物有不同程度耐药;氨苄西林对不同菌株的MIC值在0.031~2.000μg/mL之间;所有菌株均能生成生物被膜,但生物被膜生成能力有差异;不同菌株在各自4、2、1 MIC下生物被膜的生成受到不同程度抑制,而亚抑菌浓度的氨苄西林能刺激生物被膜的生成,氨苄西林对已经生成的生物被膜不能完全清除。结果表明,不同来源产气荚膜梭菌形成生物被膜前后对药物敏感性不同,可为产气荚膜梭菌的临床用药选择及使用剂量提供一定的参考依据。

关键词：产气荚膜梭菌生物被膜最小抑菌浓度耐药性

Degradation of pyrene by immobilized microorganisms in saline-alkaline soil

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Journal of Environmental Sciences 2012年第9期24卷 1662-1669页

作者： Shanxian wang Xiaojun Li wan Liu Peijun Li Lingxue Kong Wenjie Ren Haiyan wu ying Tu Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Centre for Material and Fiber Innovation Institute for Technology and Research Innovation Deakin University Geelong Vic 3217 Australia College of Resource and Environmental Engineering Liaoning Technical University Fuxin 123000 China

Biodegradation of polycyclic aromatic hydrocarbons （PAHs） is very difficult in saline-alkaline soil due to the inhibition of microbial growth under saline-alkaline stress. The microorganisms that can most effectively degrade PAHs were screened by introducing microorganisms immobilized on farm byproducts and assessing the validity of the immobilizing technique for PAHs degradation in pyrene-contaminated saline-alkaline soil. Among the microorganisms examined, it was found that Mycobacterium sp. B2 is the best, and can degrade 82.2% and 83.2% of pyrene for free and immobilized cells after 30 days of incubation. The immobilization technique could increase the degradation of pyrene significantly, especially for fungi. The degradation of pyrene by the immobilized microorganisms Mucor sp. F2, fungal consortium MF and co-cultures of MB＋MF was increased by 161.7% （P 〈 0.05）, 60.1% （P 〈 0.05） and 59.6% （P 〈 0.05） after 30 days, respectively, when compared with free F2, MF and MB＋ME Scanning electron micrographs of the immobilized microstructure proved the positive effects of the immobilized microbial technique on pyrene remediation in saline- alkaline soil, as the interspace of the carder material structure was relatively large, providing enough space for cell growth. Co-cultures of different bacterial and fungal species showed different abilities to degrade PAHs. The present study suggests that Mycobacterium sp. B2 can be employed for in situ bioremediation of PAHs in saline-alkaline soil, and immobilization of fungi on farm byproducts and nutrients as carriers will enhance fungus PAil-degradation ability in saline-alkaline soil.

关键词： saline-alkaline soil immobilization PAHs-degrading microorganisms biodegradation characteristics Mycobacterium

Review of vanadium-based electrode materials for rechargeable aqueous zinc ion batteries

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Journal of Energy Chemistry 2021年第5期30卷 223-237页

作者： ying Liu Xiang wu School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870LiaoningChina wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074HubeiChina

In recent years,rechargeable aqueous zinc ion batteries(ZIBs),as emerging energy storage devices,stand out from numerous metal ion *** to the advantages of low cost,environmentally friendly characteristic and safety,ZIBs can be considered as alternatives to lithium-ion batteries(LIBs).Vanadiumbased compounds with various structures and large layer spacings are considered as suitable cathode candidates for *** this review,the recent research advances of vanadium-based electrode materials are systematically *** electrode design strategy,electrochemical performances and energy storage mechanisms are ***,we point out the limitation of vanadium-based materials at present and the future prospect.

关键词： Aqueous zinc ion batteries Vanadium-base compounds Cathode materials Energy storage mechanism

Coupled Influence of Temperature and Strain Rate on Tensile Deformation Characteristics of Hot-Extruded AZ31 Magnesium Alloy

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Acta Metallurgica Sinica(English Letters) 2016年第2期29卷 163-172页

作者： ying Yan wan-Peng Deng Zhan-Feng Gao Jing Zhu Zhong-Jun wang Xiao-wu Li Institute of Materials Physics and Chemistry School of Materials Science and Engineering Northeastern University College of Materials and Metallurgy Liaoning University of Science and Technology Key Laboratory for Anisotropy and Texture of Materials Ministry of Education Northeastern University

To explore the coupled effect of temperature T and strain rate ε on the deformation features of AZ31 Mg alloy, mechanical behaviors and microstructural evolutions as well as surface deformation and damage features were system- atically examined under uniaxial tension at T spanning from 298 to 523 K and ε from 10^-4 to 10^-2 s-1. The increase in T or the decrease in ε leads to the marked decrease in flow stress, the appearance of a stress quasi-plateau after an initially rapid strain hardening, and even to the occurrence of successive strain softening. Correspondingly, the plastic deformation modes of AZ31 Mg alloy transform from the predominant twinning and a limited amount of dislocation slip into the enhanced non-basal slip and the dynamic recrystallization （DRX） together with the weakened twinning. Meanwhile, the cracking modes also change from along grain boundaries （GBs） and at twin boundaries （TBs） or the end of twins into nearby GBs where the DRX has occurred. The appearance of a stress quasi-plateau, the formation of large-sized cracks nearby GBs, and the occurrence of continuous strain softening, are intimately related to the enhancement of the non-basal slip and the DRX.

关键词： AZ31 Mg alloy Uniaxial tension Temperature Strain rate Deformation Damage Twinning