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Comparative transcriptome analysis of different heat stress responses between self-root grafting line and heterogeneous grafting line in rose

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Horticultural Plant Journal 2021年第3期7卷 243-255页

作者： Wenquan Qi Chunling Zhang Wenjing wang Zhe Cao Song Li Hang Li wan zhu Yongqi Huang Manzhu Bao Yanhong He Riru Zheng Key Laboratory of Horticultural Plant Biology Ministry of EducationCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Urban Agriculture in Central China Ministry of AgricultureWuhan 430070China Crop Development Centre Development of Plant SciencesUniversity of SaskatchewanSaskatoon S7N5A8Canada

Rose is sensitive to high temperature which will make the rose go into a semi-dormancy *** is an excellent way to enhance rose heat ***,heat-tolerant Rosa multiflora‘Huanong Wuci 1'(W)and heat-sensitive Rosa chinensis‘Old Blush’(X)were selected as experimental *** RNA-seq technique was used to investigate the transcriptomes of self-root grafting line(XX0),heterogeneous grafting line(XW0),self-root grafting line under 6 h heat stress(XX6),and heterogeneous grafting line under 6 h heat stress(XW6).Under high temperature stress,multiple signaling pathways were activated,moreover,a large number of transcription factors and functional genes were induced,especially the HSFs and HSPs with remarkably upregulated expression *** GO analysis showed that the differences in the expressions of the genes related to fatty acids and carbohydrates were observed between self-root grafting line and heterogeneous grafting *** addition,14 P450s were differentially expressed,and one lectin gene was up-regulated in XW0 vs XW6,but down-regulated in XX0 vs *** physiological and biochemical traits such as relative electrolyte leakage,SOD activity,proline,and total soluble sugars,DEGs involved in these processes may be key factors to resist high *** present study provides an insight into the complex mechanism underlying grafting in response to heat *** results indicate that grafting is an effective way to improve rose heat resistance.

关键词： Rose Heat stress Grafting Transcriptome

Influence of ion size on the charge storage mechanism of MXenes:a combination of experimental and computational

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Waste Disposal and Sustainable Energy 2024年第2期6卷 163-171页

作者： Huiwen wan Rui wang Huiyang Fan Hongbo Gao Yucheng Chen zhu Liu State Key Laboratory of Clean Energy Utilization Institute for Thermal Power EngineeringCollege of Energy EngineeringZhejiang UniversityHangzhou 310027China China North Vehicle Research Institute No.4 HuaishulingFengtai DistrictBeijingChina ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China

MXene nanomaterials have attracted great interest as the electrode of ***,its energy storage mecha-nisms in organic electrolytes are still *** work investigated the size effect of cations(i.e.,Lit,Nat,K^(+),and EMIM^(+))on the capacitive behaviors of MXene-based *** experimental results demonstrate that the specific capacitance increases obviously with decreasing cation size(i.e.,from 43 F g^(-1)(EMIM^(+))to 129 F g^(-1)(Li^(+))at 2 mV s^(-1)).Density-functional theory calculation reveals a correlation between cation size and ion-electrode surface interaction,sup-porting experimental observations of the capacitive-dominant *** dynamics simulations reveal that the ionic solvation structure and desolvation degree of intercalated cations as a function of solvation size,providing dynamic insights into the experimentally observed specific capacitance *** comprehensive experimental and computational study provides valuable insights into the intricate solvation effects governing the charge storage *** finding of ion dynamics,solvation structure,and desolvation may contribute to guide the design and optimization of appropriate ions/electrolytes combinations for MXene-based supercapacitors.

关键词： MXene Cations Molecular dynamics simulation Density-functional theory calculation Supercapacitors

Mechanism of AMPK modulated NLRP3 inflammasome activation to induce hepatic stellate cells pyroptosis

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中国药理学与毒理学杂志 2019年第10期 907-908页

作者： GUO Wen-ying LIU Jia LIU Min LI Jin-zhuo zhu wan-wan YANG Yong BAI Ting Medical College of Dalian University

OBJECTIVE To investigate the mechanism of adenosine monophosphate-activated protein kinase（AMPK） modulated NLRP3 inflammasome activation to induce hepatic stel ate cel s（HSCs） pyroptosis. METHODS In vivo, liver fibrosis was induced by intraperitoneal injections of thioacetamide(TAA, 200 mg·kg-1) three weekly for 5 weeks in male BALB/c mice. The administration of AICAR(AMPK activator, 500 mg·kg-1) was started fol owing TAA injections and was continued for 5 weeks to evaluate the effects by hematoxylin and eosin（H&E） staining, the levels of serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST）; the protein and m RNA extracted from liver tissue were analyzed by Western blotting analysis and Real-time Reverse Transcription Polymerase Chain Reaction（RT-PCR）. In vitro, human hepatic stellate cell line, LX-2 cells were treated with AICAR(500 μmol · L-1) and MCC950(NLRP3 inhibitor,10 μmol·L-1) prior to ATP for 24 h. AMPK, p-AMPK, NLRP3,collagen-I and α-smooth muscle actin（α-SMA） protein expressions and m RNA levels for 24 h were analyzed by Western blotting and RT-PCR. Moreover, interleukin（IL）-1β level was investigated by ELISA and cells pyroptosis was detected by propidiumiodide（PI）. RESULTS In vivo,our data demonstrated that treated with AICAR obviously improved tissue damage compared with TAA-only group,in that less inflammatory cell infiltration was observed in H&E staining results. AICAR markedly reduced the serum ALT and AST activities. AICAR demonstrated great efficacy in curing hepatic fibrosis via attenuating the TAAmediated collagen-Ⅰ and α-SMA. AICAR significantly inhibited the expression of NLRP3 and activated AMPK phosphorylation. Furthermore, AICAR significantly decreased the levels of IL-1β in TAA-induced mouse hepatic *** vitro, AICAR and MCC950 attenuated collagen-Ⅰ andα-SMA expression, activated AMPK phosphorylation in ATP treated LX-2 cells. Meanwhile, AICAR and MCC950 inhibited the expression of NLRP3 mediated by ***

关键词： hepatic stellate cells pyroptosis inflammasome AMPK NLRP3

Formation of Inclusion in Ti–Al Killed Low C–Mn Steel

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Journal of Iron and Steel Research International 2014年第S1期21卷 87-90页

作者： Cheng-wei YANG Xin-hua wanG Tie OU Jiang-hua QI wan-jun zhu Jun-fu CHEN Li-ping LIN Research and Development Center Wuhan Iron and Steel (Group) Corp. School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Research and Development Center Wuhan Iron and Steel (Group) Corp. Wuhan 430080 Hubei China

The formation of inclusion in Ti–Al complex deoxidized C–Mn steel was investigated. When Al content in steel is very low([Al]=0.0005%), for 0.003%0.006%, inclusions would be pure Al2O3. The experimental results agre... 详细信息

关键词： Ti-killed steel oxide metallurgy inclusion

Synthesis of 2, 6-(substituded)pyridine Derivatives Using Amide and Imine Groups

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Chinese Chemical Letters 2003年第6期14卷 572-574页

作者： Mei Ying LI, Pei Zhi HU*, wan Ren zhu, Kuo Xi XU College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072 Coll. of Chem. and Molecular Science Wuhan University Wuhan 430072 China

A new wo-armed?acyclic diamide Ia 2, 6-bis(1-ethanecarbozamido-2-amino)pyridine, and a new series of aromatic aldehyde schiff bases containing pyridine ring and amide bridge, IIa-f, were prepared. The compounds were characterized by elemental analysis, IR, 1HNMR and MS. The bioactivity half inhibitory concentration C1/2 is given.

关键词： Aromatic aldehyde Schiff bases pyridine amide bridge.

Boosting thermal stability and crystallization of closed-loop-recyclable biodegradable poly(p-dioxanone) by end-group regulation

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Science China Chemistry 2024年第2期67卷 642-651页

作者： Jin Lei zhu-Xin Gui wan-Ting Xiong Gang Wu Si-Chong Chen Yu-Zhong wang The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials(MoE) National Engineering Laboratory of Eco-Friendly Polymeric Materials(Sichuan)State Key Laboratory of Polymer Materials EngineeringCollege of ChemistrySichuan UniversityChengdu610064China

A chemically closed-loop-recyclable biodegradable polymer,poly(p-dioxanone)(PPDO),is one of the ideal candidates for single-use plastic products due to its suitability for different application ***,when PPDO wastes can be collected,its monomer p-dioxanone(PDO) will be obtained through chemical recycling of these wastes;when cannot be collected,the wastes are able to be biodegraded into harmless ***,unsatisfied thermal stability and low crystallization rate of PPDO restrict its wider ***,based on end-group regulation,we simultaneously realized the significant enhancement of thermal stability and crystallization of PPDO through the simple melt processing with tributyl phosphite(TBP) or triphenyl phosphite(TPP).The model reactions were conducted to investigate the reaction mechanism and theoretical products during the preparation of PPDO/phosphite *** kinds of phosphites were proved to act as the end-capped reagent and chain extender in the melt processing,while TBP presented better *** a result,the activation energy of thermal decomposition was largely elevated,and the unprecedented T_(5%)(the temperature at a weight loss of 5%) and Tmax(the temperature at a maximum rate of weight loss) of PPDO were obtained,i.e.,T_(5%)of ~330 ℃ and Tmaxof ~385 ℃ in N_(2) atmosphere,T_(5%)of ~240 ℃ and Tmaxof ~317 ℃ in air atmosphere,***,the increased crystallization rate,crystallinity,crystalline orderliness,and realizable monomer recovery(yield >90%,purity >99.9%) of PPDO/phosphite compounds were confirmed.

关键词： poly(p-dioxanone) phosphite thermal stability crystallization end-group regulation

Evolution of interfacial heat transfer,contact behavior and microstructure during sub-rapid solidification of molten steel with different hydrogen contents

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Journal of Iron and Steel Research International 2024年第1期31卷 215-223页

作者： Cheng Lu wan-lin wang Chen-yang zhu Jie Zeng Xin-yuan Liu Hua-long Li School of Metallurgy and Environment Central South UniversityChangsha410083HunanChina National Center for International Research of Clean Metallurgy Central South UniversityChangsha410083HunanChina Institute of Research of Iron and Steel ShasteelZhangjiagang215625JiangsuChina

Typical Q235 low-carbon steel samples with different hydrogen contents(0.0004,0.0008,and 0.0013 wt.%)were prepared by adjusting the environment humidity and *** effects of hydrogen on interfacial heat transfer,contact behavior,and microstructure evolution were investigated using a novel droplet solidification *** results revealed that when the hydrogen content increases from 0.0004 to 0.0013 wt.%,the maximum heat flux between the molten steel and cooling substrate decreases from 8.01 to 6.19 MW/m^(2),and the total heat removed in the initial 2 s reduces from 10.30 to 8.27 MJ/m^(2).Moreover,the final contact angle between the molten steel and substrate increases from 103.741°to 113.697°,and the number of pores on the droplet bottom surface increases significantly from 21 to 210 with the increase in *** surface roughness of the droplet bottom surface increases from 20.902 to 49.181 *** addition,the average grain size of the droplet increases from 14.778 to 33.548 pm with the increase in the hydrogen *** interfacial contact condition becomes worse due to the escape of hydrogen from the steel matrix during the cooling process,which leads to the reduction in the interfacial heat transfer and the increase in the grain size.

关键词： Strip casting Hydrogen content Interfacial heat transfer behavior Contact behavior Microstructure evolution

Parameter calibration of the tensile-shear interactive damage constitutive model for sandstone failure

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第4期16卷 1153-1174页

作者： Yun Shu Zheming zhu Meng wang Weiting Gao Fei wang Duanying wan Yuntao wang MOE Key Laboratory of Deep Earth Science and Engineering College of Architecture and EnvironmentSichuan UniversityChengdu610065China Failure Mechanics and Engineering Disaster Prevention Key Laboratory of Sichuan ProvinceSichuan UniversityChengdu610065China Tianfu Engineering-oriented Numerical Simulation & Software Innovation Center Chengdu620107China

The tensile-shear interactive damage(TSID)model is a novel and powerful constitutive model for rock-like *** study proposes a methodology to calibrate the TSID model parameters to simulate *** basic parameters of sandstone are determined through a series of static and dynamic tests,including uniaxial compression,Brazilian disc,triaxial compression under varying confining pressures,hydrostatic compression,and dynamic compression and tensile tests with a split Hopkinson pressure *** on the sandstone test results from this study and previous research,a step-by-step procedure for parameter calibration is outlined,which accounts for the categories of the strength surface,equation of state(EOS),strain rate effect,and *** calibrated parameters are verified through numerical tests that correspond to the experimental loading *** between numerical results and experimental data indicates the precision and reliability of the calibrated *** methodology presented in this study is scientifically sound,straightforward,and essential for improving the TSID ***,it has the potential to contribute to other rock constitutive models,particularly new user-defined models.

关键词： Damage constitutive model Parameter calibration Rock modeling Sandstone Dynamic impact load Tensile-shear interactive damage(TSID)model

Research progress on rolling superlubricity in solid lubricants

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Science China(Technological Sciences) 2024年第7期67卷 1980-1990页

作者： LI PanPan LI HongXuan zhu DongXiang zhu YongQi KANG FuYan wanG wan ZHANG YaXin LIU XiaoHong JI Li ZHOU HuiDi CHEN JianMin State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China School of Chemical Engineering Northwest Minzu UniversityLanzhou 730030China

Superlubricity is an ideal lubrication state where friction nearly vanishes between contact interfaces. It has become one of the most important research topics and approaches owing to its significance in reducing energy consumption and preventing device failures. As an efficient and universal lubricating principle capable of achieving superlubricity, rolling lubrication has attracted widespread attention in recent years. In this review, the theoretical concept of rolling lubrication and the experimental research progress of spherical/scroll structures are summarized. The review focuses on the possibility of achieving rolling lubrication using spherical/scroll structures(such as spherical fullerenes, carbon nanotubes, and formed and constructed spherical/scroll structures). The challenges in achieving rolling lubrication are summarized, and the possibility of molecular rolling lubrication,as well as its potential applications in superlubrication, are discussed.

关键词： spherical/scroll structure rolling lubrication superlubricity fullerene