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Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 959-963页

作者： Wanigasekera Daya Ratnasooriya walimuni prabhashini kaushalya mendis abeysekera Chatura Tissa Dayendra Ratnasooriya Faculty of Allied Health Sciences General Sir John Kothalawala Defence University Herbal Technology Section Industrial Technology Institute363Bauddhaloka MawathaColombo 07Sri Lanka Faculty of Medicine University of Colombo

Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe（OP） grade black tea with a view to develop an anti-aging skin ***:Five concentrations（0.125,0.250,0.500,1.000 and 2.000 mg/mL） of black tea brew（BTB） were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization *** gallate（EGCG） was used as the reference agent（concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL）.Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid（from rooster comb）and bovine testicular ***:The results revealed that BTB had moderate[IC50=（1.09±0.12） mg/mL]and dose dependent（r2=0.94） anti-hyaluronidase *** also exhibited dose dependent（r2=0.93,P<0.05） anti-hyaluronidase activity which was superior[IC50=（0.09±0.00） mg/mL]to ***:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging *** addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.

关键词： Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid

Antioxidant potential of brans of twenty-nine red and white rice (Oryza sativa L.) varieties of Sri Lanka

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Journal of Coastal Life Medicine 2017年第11期5卷 480-485页

作者： walimuni Kanchana Subhashini mendis abeysekera Udawaththa Kankanamge Don Sahan Suganda Gunasekara Sirimal Premakumara Galbada Arachchige walimuni prabhashini kaushalya mendis abeysekera Department of Agricultural Technology Faculty of TechnologyUniversity of ColomboSri Lanka Food Technology Section(FTS) Modern Research&Development Complex(MRDC)Industrial Technology Institute(ITI)503AHalbarawa GardensMalabeSri Lanka Herbal Technology Section(HTS) Modern Research and Development Complex(MRDC)Industrial Technology Institute(ITI)503AHalbarawaGardensMalabeSri Lanka

Objective: To evaluate antioxidant properties of brans of twenty-nine red and white rice varieties of Sri Lanka. Methods: Brans of 21 new improved (NI), 2 old improved (OI) and 6 traditional red and white rice varieties of Sri Lanka were studied for range of antioxidant properties. The studied antioxidant properties included total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging activity in vitro. Bran of black rice variety from Korea was also studied for the same antioxidant properties for comparison. Results: Results exhibited significantly high ABTS and DPPH radical scavenging activities and 10, 7 and 2.5 fold greater TPC, FRAP and ORAC activities in brans of red rices (BRRs) compared to brans of white rices irrespective of NI, OI and traditional rice types. Among BRRs traditional varieties had greater ABTS and DPPH radical scavenging activities and 1.7, 1.3 and 1.2 fold respectively greater TPC, FRAP and ORAC in contrast to NI red rices. Traditional red rice varieties, Kalu Heeneti (TPC and ORAC), Pachchaperumal (TPC and DPPH) and Kurulu Thuda (DPPH) and OI red rice variety H4 (FRAP) exhibited the highest activities for the antioxidant properties studied. Further, these varieties had significantly high activities compared to black rice. Conclusions: In conclusion, BRRs especially traditional red rices had greater antioxidant properties and consumption may be useful in managing various chronic diseases.

关键词： Antioxidant properties Rice bran Sri Lankan rice Red and white rice New improved,old improved and traditional rice

In vitro pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea(Camellia sinensis L.)

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Journal of Coastal Life Medicine 2015年第7期 570-574页

作者： Wanigasekera Daya Ratnasooriya walimuni prabhashini kaushalya mendis abeysekera Chatura Tissa Dayendra Ratnasooriya Faculty of Allied Health Sciences General Sir John Kothalawala Defence UniversityRatmalanaSri Lanka Department of Zoology University of ColomboColombo-03Sri Lanka Herbal Technology Section Industrial Technology Institute(ITI)363Bauddhaloka MawathaColombo 07Sri Lanka Faculty of Medicine University of ColomboColombo-08Sri Lanka

Objective:To access the pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds of Camellia sinensis ***:Black tea brew(BTB)was made according to International Organization for Standardization 3103 specifications and concentrations of BTB tested were 37.5,75.0,150.0,300.0 and 600.0μg/mL for antilipase and anti-cholesterol esterase assays and 0.25,0.50 and 1.00μg/mL for cholesterol micellization inhibitory ***:The results showed that BTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent(r^(2)=0.95)cholesterol micellization inhibitory activity in vitro comparable to epigallocatechin gallate,the reference drug *** contrast,BTB had only mild but dose-dependent(r^(2)=0.94)inhibitory activity against pancreatic lipase and weak inhibitory effect(up to 13.17%)on pancreatic cholesterol ***:It is concluded that consumption of BTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.

关键词： Black tea Orange Pekoe Antilipase Anti-cholesterol esterase Cholesterol micellization Hyperlipidaemia Camellia sinensis

Sri Lankan black tea(Camellia sinensis L.)inhibits the methylglyoxal mediated protein glycation and potentiates its reversing activity in vitro

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Journal of Coastal Life Medicine 2016年第2期4卷 148-153页

作者： walimuni Kanchana Subhashini mendis abeysekera Chatura Tissa Dayendra Ratnasooriya Wanigasekara Daya Ratnasooriya Sirimal Premakumara Galbada Arachchige Industrial Technology Institute(ITI) 363Bauddhaloka MawathaColombo 07Sri Lanka Faculty of Medicine University of ColomboColombo 08Sri Lanka Department of Basic Sciences Faculty of Allied Health SciencesGeneral Sir John Kotalawala Defense UniversityRatmalanaSri Lanka

Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black ***:Freeze dried black tea brew(BTB)was used as the sample in this *** and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO *** properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant ***:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO ***:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.

关键词： Methylglyoxal Advanced glycation end products Glycation reversing Diabetes complications Orange pekoe grade black tea Sri Lankan tea

Physicochemical and nutritional properties of twenty three traditional rice (Oryza sativa L.) varieties of Sri Lanka

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Journal of Coastal Life Medicine 2017年第8期5卷 343-349页

作者： walimuni Kanchana Subhashini mendis abeysekera Sirimal Premakumara Galbada Arachchige Wanigasekara Daya Ratnasooriya Naduviladath Vishvanath Chandrasekharan Amitha Priyadarshanie Bentota Food Technology Section(FTS) Modern Research&Development Complex(MRDC)Industrial Technology Institute(ITI)503AHalbarawa GardensMalabeSri Lanka Herbal Technology Section(HTS) Modern Research&Development Complex(MRDC)Industrial Technology Institute(ITI)503AHalbarawa GardensMalabeSri Lanka3Department of Basic SciencesFaculty of Allied Health SciencesGeneral Sir John Kotalawala Defense UniversityRatmalanaSri Lanka Department of Chemistry Faculty of ScienceUniversity of ColomboColomboSri Lanka Rice Breeding Division Rice Research and Development Institute(RRDI)BathalagodaSri Lanka

Objective: To evaluate the physicochemical and nutritional properties of selected traditional rice varieties of Sri Lanka. Methods: Twenty three rice varieties were selected. All the varieties were studied for grain colour, grain size and shape as physicochemical properties, while for nutritional properties, moisture, crude ash, crude protein, crude fat and total carbohydrate contents were studied. Only selected set of varieties were studied for total, soluble and insoluble dietary fiber. These properties were studied using internationally accepted standard techniques. Results: The results showed significant (P < 0.05) difference in the physicochemical and nutritional properties of the studied varieties. Out of 23 varieties tested, 20 varieties were red rices, while the rest were white rices. The grain size and shape were mostly medium bold (30%) and short bold (56%). The mean moisture, crude ash, crude protein, crude fat and total carbohydrate contents of selected varieties varied from (10.42 ± 0.25)% to (12.33 ± 0.02)%, (1.30 ± 0.14)% to (1.92 ± 0.05)%, (10.59 ± 0.12)% to (13.27 ± 0.32)%, (2.18 ± 0.10)% to (4.12 ± 0.28)% and (81.42 ± 0.25)% to (85.66 ± 0.24)%, respectively. The selected varieties had total, soluble and insoluble dietary fiber contents in the range of 4.2%–6.9%, 0.8%–2.1%and 3.1%–4.8%, respectively. The varieties Pachchaperumal, Suduru samba, Wanni Dahanala, Gonabaru and Sudu Heeneti had the highest crude protein, crude fat, crude ash, total carbohydrate and dietary fiber contents, respectively. Conclusions: Selected traditional rice varieties of Sri Lanka had physicochemical properties preferred by consumers and nutritional properties with dietary importance.

关键词： Rice Sri Lankan rice Traditional rice Physicochemical properties Nutritional properties

Anti-diabetic related health food properties of traditional rice(Oryza sativa L.)in Sri Lanka

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Journal of Coastal Life Medicine 2015年第10期 815-820页

作者： walimuni Kanchana Subhashini mendis abeysekera Sirimal Premakumara Galbada Arachchige Wanigasekara Daya Ratnasooriya Muhammad Iqbal Choudhary Kourosh Dalvandi Naduviladath Vishvanath Chandrasekharan Industrial Technology Institute(ITI) 363Bauddhaloka MawathaColombo 07Sri Lanka Faculty of Science University of ColomboColomboSri Lanka Faculty of Allied Health Sciences General Sir John Kothalawala Defence UniversityRatmalanaSri Lanka Dr.Panjwani Center for Molecular Medicine and Drug Research and H.E.J.Research Institute of Chemistry International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270Pakistan

Objective:To evaluate a range of anti-diabetic related properties and some consumer preferred physicochemical properties of selected Sri Lankan traditional rice ***:Sudu Heeneti,Goda Heeneti,Masuran and Dik Wee varieties were used in this ***-diabetic related properties of bran extracts of selected varieties were studied for methylglyoxal mediated protein glycation inhibition,acetyl and butyryl-cholinesterase inhibition in vitro and anti-hyperglycemic activity in ***,selected varieties were studied for starch hydrolysis rate in *** properties including grain color,size,shape,crude protein,crude fat,ash,dietary fiber and total carbohydrate contents were ***:Brans of selected varieties had significant(P<0.05)and dose dependent methylglyoxal mediated protein glycation inhibition[IC_(50):(174.77±6.65)to(342.87±0.43)μg/mL]and acetyl[IC_(50):(37.00±0.68)to(291.00±3.54)μg/mL]and butyryl-cholinesterase[IC_(50):(18.50±0.60)to(96.60±0.56)μg/mL]inhibitory ***,Sudu Heeneti,Masuran and Dik Wee had low starch digestion rate(52.40±1.44 to 53.76±1.19)indicating that these varieties may be low glycemic index *** of Masuran tested in rat model showed anti-hyperglycemic *** properties studied showed that selected varieties were red in color and grain size and shape were mostly medium and bold ***,crude protein,crude fat,ash and total carbohydrate contents varied significantly(P<0.05)among the ***:It is concluded that selected varieties could be promoted as physicochemically sound rices with a range of anti-diabetic related properties in the management of diabetes and its complications.

关键词： Anti-diabetic related health food properties Traditional rice Sri Lankan rice Rice bran Physicochemical properties