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In vitro antitumor efficacy of Kochia indica extract on human hepatocellular carcinoma cell line with or without 5-fluorouracil

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Hepatoma Research 2017年第7期3卷 149-155页

作者： Nabil Mohie Abdel-Hamid Ghada A.Tabl Yousry EEl-Bolkiny walaa o.zeina Department of Biochemistry Faculty of PharmacyKafrelsheikh UniversityKafrelsheikh33516Egypt Department of Zoology Faculty of ScienceTanta UniversityTanta71561Egypt.

Aim: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and the sixth most common cancer worldwide. The resistance to chemotherapy is a major obstacle in the treatment of HCC, necessitating the discovery of additional agents. There is a growing use of anticancer complementary and alternative medicine worldwide. Therefore, the aim of present study was focused on the confirmation of the suitability and validity of the new markers which would be achieved by demonstrating their significant change and reproducible expression during disease and disease management. Methods: HepG2 cell line was used to provide a source for HCC cells. The cell cultures were divided into 4 groups: control untreated group, 5-fluorouracil (5-FU) treated group as a standard chemotherapy for HCC (positive control) with the following doses (15.625, 31.2, 62.5, 125, 250 μg/mL), Kochia indica extract treated group with the following concentration (12.5, 25, 50, 100, 200 μg/mL) and the group treated with a combination of 5-FU and Kochia indica in different ratios. Results: Treatment with Kochia indica extract, 5-FU and the combined treatment showed a significant cytotoxicity to HepG2 cells, with different IC50 values, when compared to the control. Regarding toxic effect, 5-FU showed IC50 = 237.56 μg/mL which is lower cytotoxic in compared to Kochia indica with IC50 =120.5 μg/mL. The results also revealed that tumor cells were more resistant to 5-FU. Alternatively, the co-treatment with Kochia indica extract ameliorated the toxicity induced by 5-FU and enhanced its therapeutic potency, either by synergistic effect of both agents and/or due to its flavonoid components that may enhance the physiological properties of the cell membranes, facilitating 5-FU entrance into tumor cells. This decreased its therapeutic dose to less than 250 μg/mL by combination therapy. Conclusion: Present findings assume that Kochia indica extract co-therapy can ameliorate the side effects of 5-FU on He

关键词： Hepatocellular carcinoma Kochia indica 5-fluorouracil cells of hepatoma cell line chemosensitization

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Vitamin D supplementation improves sustained virologic response in chronic hepatitis C (genotype 1)-nave patients

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World Journal of Gastroenterology 2011年第47期17卷 5184-5190页

作者： Saif Abu-Mouch Zvi Fireman Jacob Jarchovsky Abdel-Rauf zeina Nimer Assy Liver Unit Department of Internal Medicine B Hillel Yaffe Medical Center Department of Gastroenterology Hillel Yaffe Medical Center Liver Unit Hillel Yaffe Medical Center Liver Unit Ziv Medical Center Technion Institute

AIM: To determine whether adding vitamin D, a potent immunomodulator, improves the hepatitis C virus (HCV) response to antiviral therapy. METHoDS: Seventy-two consecutive patients with chronic HCV genotype 1 were randomized into two groups: the treatment group (n = 36, 50% male, mean age 47 ± 11 years) received Peg-α-2b interferon (1.5 μg/kg per week) plus ribavirin (1000-1200 mg/d) together with vitamin D3 (2000 IU/d, target serum level > 32 ng/mL), and the control group (n = 36, 60% male, mean age 49 ± 7 years) received identical therapy without vitamin D. HCV-RNA was assessed by realtime polymerase chain reaction (sensitivity, 10 IU/mL). The sustained virologic response (SVR) was defined as undetectable HCV-RNA at 24 wk post-treatment. RESULTS: Clinical characteristics were similar in both groups. The treatment group had a higher mean bodymass index (27 ± 4 kg/m2 vs 24 ± 3 kg/m2, P oad (50% vs 42%, P osis score (> F2: 42% vs 19%, P ontrols. At week 4, 16 (44%) treated patients and 6 (17%) controls were HCV-RNA negative (P ontrols were HCV-RNA negative (P ost-treatment (SVR), 31 (86%) treated patients and 15 (42%) controls were HCV-RNA negative (P oad, advanced fibrosis and vitamin D supplementation were strongly and independently associated with SVR (multivariate analysis). Adverse events were mild and typical of Peg-α-2b/ribavirin. CoNCLUSIoN: Adding vitamin D to conventional Peg-α-2b/ribavirin therapy for treatment-na■ve patients with chronic HCV genotype 1 infection significantly improves the viral response.

关键词： Hepatitis C Vitamin D Sustained viral response Genotype 1 Fibrosis

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Changes in Pleurotus ostreatus nutritional value and heavy metal profile as a result of supplementation with nano-additive

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Studies in Fungi 2022年第1期7卷 89-94页

作者： Youssef Najib Sassine Nevena Shuleva zeina El Sebaaly Department of Plant Production Faculty of AgricultureLebanese UniversityBeirutLebanon Department of Agronomy Faculty of AgronomyUniversity of ForestrySofiaBulgaria

The present study evaluated the effect of Lithovit-Amino25 on the nutrient profile and heavy metal composition of Pleurotus *** product was tested in two doses applied at three different timings:T2:3 g kg^(−1)/spawning,T3:3 g kg^(−1)/after first harvest,T4:3 g kg^(−1)/spawning and after first harvest,T5:5 g kg^(−1)/spawning,T6:5 g kg^(−1)/after first harvest,and T7:5 g kg^(−1)/spawning and after first *** to control(T1:non-treated substrate),mushrooms’fibers and carbohydrates increased in all treatments,recording the highest values in T4(4.16%)and T3(18.42%),*** content was higher in mushrooms of substrates treated at spawning,with a 0.33%improvement in *** content decreased in T3,T4,T6,and *** sugars decreased in mushrooms of treated substrates,and glucose was the dominant sugar in *** increased in mushrooms of T3 and ***,iron,and potassium decreased in mushrooms of treated *** decreased in T3,T5,and T7,magnesium increased in T2,and phosphorus increased only in T2 and *** content of all treated mushrooms was in the standard safe limit(ound 2.5,6.6,and 5.1 ppm,compared to ***,zinc content increased in mushrooms of all treated substrates,and nickel and lead,increased by respective ranges of 2.8−11.88 ppm and 9.1−21 ppm,higher than the safe *** product presented a risk of heavy metal bioaccumulation even with a low dose.

关键词： content sugar additive

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Antioxidative, cytotoxic, and anti-metastatic potentials of Laurencia obtusa and Ulva lactuca seaweeds

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Asian Pacific Journal of Tropical Biomedicine 2021年第7期11卷 308-316页

作者： Reem Al Monla Yahya Salma Achraf Kouzayha Hala Gali-Muhtasib zeina Dassouki Hiba Mawlawi Laboratory of Applied Biotechnology(LBA3B) AZM Center for Research in Biotechnology and its ApplicationsDoctoral School for Sciences and TechnologyLebanese UniversityTripoliLebanon Department of Biology American University of BeirutLebanon Center for Drug Discovery American University of BeirutRiad El Solh 11072020BeirutLebanon

objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell ***:The antioxidant radical scavenging activity of the algal extracts was estimated using the 2,2-diphenyl-1-picrylhydrazyl colorimetric ***,the cytotoxic potential of these bioactive extracts was studied against HCT-116 and HeLa cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide *** healing analysis was adopted to evaluate the antimetastatic effects of protein extracts from both *** cycle analysis was performed by flow cytometry,and apoptotic bodies were detected using 4′,6-diamidino-2-phenylindole fluorescent *** acids of hydrolyzed protein extract were separated and identified chromatographically by high performance liquid ***:The green algae Ulva lactuca had higher lipid content than Laurencia obtusa,whereas the latter had higher protein content with high antioxidant *** extracts had significant dose-and time-dependent cytotoxicity and anti-metastatic activity against HCT-116 *** b extracts of both algae obtained from the chloroform:hexane solvent lipid-free residue caused morphological changes and induced apoptosis of HCT-116 *** analysis revealed that apoptosis induced upon Laurencia obtusa protein b treatment was triggered via the RoS pathway,causing a significant increase in the sub-G_(1) *** and arginine(5.94 and 5.47μM,respectively)were the most common amino acids in Laurencia obtusa protein b ***:Proteins of Laurencia obtusa and Ulva lactuca could be considered as an adjuvant nutraceutical source of pro-apoptotic and anti-metastatic biomolecules against colon cancer.

关键词： Macroalgae Laurencia obtusa Ulva lactuca Reactive oxygen species Apoptosis

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Charcot-Marie-Tooth-1A and sciatic nerve crush rat models:insights from proteomics

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Neural Regeneration Research 2023年第6期18卷 1354-1363页

作者： zeina Msheik Stephanie Durand Emilie Pinault Martial Caillaud Laetitia Vignaud Fabrice Billet Mohamed El Massry Alexis Desmoulière UR20218 NeurIT(NEURopathies périphériques et Innovation Thérapeutique) University of LimogesLimogesFrance BISCEm(Biologie Intégrative SantéChimie Environnement)Platform US 42 Inserm/UAR 2015 CNRSUniversity of LimogesLimogesFrance UMR 1308 Inserm/CHU–CAPTuR(Contrôle de l’Activation cellulaire Progression Tumorale et Résistance thérapeutique)University of LimogesLimogesFrance Inserm UMR1235–TENS(The Enteric Nervous System in Gut and Brain Diseases) University of NantesNantesFrance

The sensorimotor and histological aspects of peripheral neuropathies were already studied by our team in two rat models:the sciatic nerve crush and the Charcot-Marie-Tooth-1A *** this study,we sought to highlight and compare the protein signature of these two pathological ***,the identification of protein profiles in diseases can play an important role in the development of pharmacological *** fact,Charcot-Marie-Tooth-1A rats develop motor impairments that are more severe in the hind ***,for the first time,protein expression in sciatic nerve of Charcot-Marie-Tooth-1A rats was ***,distal sciatic nerves were collected from Charcot-Marie-Tooth-1A and uninjured wild-type rats aged 3 *** protein extraction,sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass spectrometry was employed.445 proteins mapped to Swiss-Prot or trEMBL Uniprot databases were identified and *** these,153 proteins showed statistically significant differences between Charcot-Marie-Tooth-1A and wild-type *** majority of these proteins were overexpressed in *** clustering and functional enrichment using Gene ontology were used to group these proteins based on their biological effects concerning Charcot-Marie-Tooth-1A ***,proteomic characterization of wild-type rats subjected to sciatic nerve crush was performed sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass *** month after injury,distal sciatic nerves were collected and analyzed as described *** of 459 identified proteins,92 showed significant differences between sciatic nerve crush and the uninjured wild-type rats used in the first *** results suggest that young adult Charcot-Marie-Tooth-1A rats(3 months old)develop compensatory mechanisms at the level of redox balance,protein folding,myelination,and

关键词： Charcot-Marie-Tooth-1A endoplasmic reticulum Gene ontology neurogenesis oxidative stress proteomics rat repair sciatic nerve crush SWATH-MS

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