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Disruption of non-classically secreted protein(MoMtp)compromised conidiation,stress homeostasis,and pathogenesis of Magnaporthe oryzae

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Journal of Integrative Agriculture 2024年第8期23卷 2686-2702页

作者： wajjiha batool Justice Norvienyeku Wei Yi Zonghua Wang Shihong Zhang Lili Lin Key Laboratory for Extreme-Environmental Microbiology College of Plant ProtectionShenyang Agricultural UniversityShenyang 110866China Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests College of Plant ProtectionHainan UniversityHaikou 570288China

Blast disease,caused by the hemibiotrophic ascomycete fungus,Magnaporthe oryzae,is a significant threat to sustainable rice production *** have shown that the blast fungus secretes vast arrays of functionally diverse proteins into the host cell for a successful disease ***,the final destinations of these effector proteins inside the host cell and their role in advancing fungal pathogenesis remain a ***,we reported that a putative mitochondrial targeting non-classically secreted protein(MoMtp)positively regulates conidiogenesis and appressorium maturation in ***,MoM TP gene deletion mutant strains triggered a hypersensitive response when inoculated on rice leaves displaying that MoMtp is essential for the virulence of *** addition,cell wall and oxidative stress results indicated that MoMtp is likely involved in the maintenance of the structural integrity of the fungus *** study also demonstrates an upregulation in the expression pattern of the MoMTP gene at all stages of infection,indicating its possible regulatory role in host invasion and the infectious development of ***,Agrobacterium infiltration and sheath inoculation confirmed that MoMtpGFP protein is predominantly localized in the host mitochondria of tobacco leaf and rice *** together,we conclude that MoMtp protein likely promotes the normal conidiation and pathogenesis of *** and might have a role in disturbing the proper functioning of the host mitochondria during pathogen invasion.

关键词： plant–pathogen interactions effectors hypersensitive response pathogenesis mitochondria

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Involvement of the Novel Transcription Factor MoTNP4 in Blast Pathogenicity by Nitrate Reduction Pathway in Magnaporthe oryzae

Involvement of the Novel Transcription Factor MoTNP4 in Blas...

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中国植物病理学会2024年学术年会

作者： FAN Xiaoning WEI Yi DANG Yuejia wajjiha batool ZHANG Shihong The Key Laboratory for Extreme-Environmental Microbiology College of Plant ProtectionShenyang Agricultural University

The nitrate reductase is vital for nitrate metabolism in most organisms,yet little is known about its pathogenic role in fungal *** this study,we identified a novel transcription factor MoTNP4 in Magnaporthe *** of MoTNP4 affected the vegetative growth,formation of conidia and conidiophore stalks,oxidative stress,melanin synthesis,virulence of *** combined analysis of RNA-seq and ChIP-seq showed that the deletion of MoTNP4 affected the transcription level of MoNR,EMSA,yeast one-hybrid and ChIP-qPCR confirmed that MoTNP4 can bind the promoter of *** addition of NaNO and overexpression of MoNR in the ΔMoTnp4 mutant restored partially its formation of conidiophore stalks and virulence,indicating that MoTNP4 could function via the nitrate reductase metabolism to regulate fungal *** together,this study uncovered a novel regulatory mechanism of fungal virulence via the nitrate reductase metabolism pathway mediated by a new transcription factor.

关键词： MoTNP4 nitrate reductase conidiophore stalks pathogenesis Magnaporthe oryzae

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Oxalic Acid in Magnaporthe oryzae:Production the Result of Erythroascorbic Acid Oxidation,Metabolism is for Fungal Detoxification

Oxalic Acid in Magnaporthe oryzae:Production the Result of E...

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中国植物病理学会2024年学术年会

作者： LIU Chang WEI Yi DANG Yuejia wajjiha batool FAN Xiaoning HU Yan HE Zhengquan ZHANG Shihong

As an essential pathogenic factor,oxalic acid（OA） has been elaborated in several plant pathogens;and researchers are currently pursuing the interference with OA metabolism as a treatment for the related ***,the metabolism route in Magnaporthe oryzae remains *** this study,we described the erythroascorbic acid mediated oxalic acid synthesis and its metabolic and clearance pathways in the rice blast *** OxdC（MoOxdC） is an oxalate decarboxylase that just appears to play a role in relieving the toxicity of oxalica *** of MoOxdC does not affect the growth,conidiophore formation,sporulation,and appressorium formation of ***,antioxidation and pathogenicity are enhanced in Δ*** of MoOxdC increased of oxalate oxidase（MoOxO） expression,which reduced the toxic effect of excess OA in *** increase of Darabino-1,4-lactone oxidase（MoALO1） expression in ΔMooxdc leads to the increase of erythroascorbic acid content and the enhancement of antioxidant *** blocking of OA decarboxylation pathway induced the expression of OA transporter to be up-regulated,which made OA transport and enhanced the pathogenic *** the same time,the loss of OA decarboxylated product-formic acid（FA） leads to the reduction of acetyl-coenzyme A,and the feedback to the TCA cycle is the acceleration of fatty acid β-oxidation pathway,which enhances the utilization capacity of ΔMooxdc to long-chain fatty *** think that the enhancement of antioxidant and pathogenicity capacity of ΔMooxdc a stress response,and this work mainly proves that the formation of OA in *** is actually the product of the removal of HO by erythroascorbic *** the perspective of ATP formation,OA mediates the carbon cycle and ensures the operation of TCA cycle.

关键词： Oxalate decarboxylase(MoOxdC) Magnaporthe oryzae erythroascorbic acid energy metabolism pathogenicity

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A short-chain acyl-CoA dehydrogenase is essential for mediating ER-β-oxidation,which is required for conidiogenesis and stress tolerance in the rice blast fungus

A short-chain acyl-CoA dehydrogenase is essential for mediat...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Sami Rukaiya Aliyu Lin Lili Waheed Abdul Chen Xiaomin Lin Yahong Frankine Jagero Otieno Ammarah Shabbir wajjiha batool Aron Osakina Tang Wei Wang Zonghua Justice Norvienyeku State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops the School of Life SciencesCollege of Plant ProtectionFujian Agriculture and Forestry University Fujian University Key Laboratory for Plant-Microbe Interaction the School of Life SciencesCollege of Plant ProtectionFujian Agriculture and Forestry University Institute of Oceanography Minjiang University

Short-chain acyl-CoA dehydrogenase(Scad) mediated β-oxidation serves as the fastest route for generating essential energies required to support the survival of organisms under stress or *** this study,we identified three putative SCAD genes in the genome of globally destructive rice blast pathogen Magnaporthe oryzae,named as MoSCAD1,MoSCAD1,and *** elucidate their function,we deployed targeted gene deletion strategy to investigate individual and the combined influence of MoSCAD genes on growth,stress tolerance,conidiation and pathogenicity of the rice blast ***,localization and co-localization results obtained from this study showed that MoScad1 localizes to the peroxisome and endoplasmic reticulum(ER),MoScad2 localize exclusively to the mitochondria while MoScad3 localize partially to mitochondria and peroxisome during all developmental stages of *** mutants showed they are essential in ER-β-*** observation showed that a minimal but significant growth reduction was caused in the deletion mutants ΔMoscad1 andΔMoscad2,while,growth characteristics exhibited by the deletion mutant ΔMoscad3 was similar with the wild-type ***,we observed that deletion of MoSCAD2 resulted in drastic reduction in conidiation,delayed germination,triggered the development of abnormal appressorium and suppressed host penetration and colonization efficiencies of the ΔMoscad1 *** study provides first material evidence confirming the possible existence of ER β-oxidation pathway in *** also infer that mitochondria β-oxidation rather than peroxisomal and ER β-oxidation play an essential role in the vegetative growth,conidiation,appressorial morphogenesis and progression of pathogenesis in ***.

关键词： ER-β-oxidation SCADs lipid peroxidation ROS M.oryzae

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