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材料科学与工程（中英文B版） 2015年第5期5卷 243-247页

作者： wagner de souza pereira Alphonse Kelecom Cleber Jabarra da Silva Master degree Course in Environmental Science Universidade Veiga de Almeida - UVA Rua lbituruna 108 Tijuca 20271-021 Rio de Janeiro R J Brazil Multidisciplinary Group ofRadioprotection (GMR) Ind~strias Nucleares do Brasil Resende 27580-970 POB:83632 Brazil Laboratory of Radiobiology and Radiometry Pedro Lopes dos Santos (LARARA-PLS) Group of Environmental Themes Assessment (GETA) Universidade Federal Fluminense (UFF) Niteroi 24001-970 POB: 100.4 36 Brazil ALARA Radioprotecao Rua Madre Maria Victoria 90sala 601 Charitas Niteroi 22370-035 R J Brasil

Investigation of Galactic open cluster remnants： the case of NGC 7193

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Research in Astronomy and Astrophysics 2017年第1期17卷 31-54页

作者： Mateus de souza Angelo Joao Francisco Coelho dos Santos Jr wagner Jose Barbosa Corradi Francisco Ferreira de souza Maia Andres Eduardo Piatti departamento de Fisica ICEx Universidade Federal de Minas Gerais Av. Antonio Carlos 6627 31270-901 Belo Horizonte MG Brazil Observatorio Astronomico Universidad Nacional de Cordoba Laprida 854 5000 Cordoba Argentina Consejo Nacional de Investigaciones Cientificas y Tecnicas Av. Rivadavia 1917 C1033AAJ Buenos AiresArgentina

Galactic open clusters （OCs） that survive the early gas-expulsion phase are gradually de- stroyed over time by the action of disruptive dynamical processes. Their final evolutionary stages are characterized by a poorly populated concentration of stars called an open cluster remnant （OCR）. This study is devoted to assessing the real physical nature of the OCR candidate NGC 7193. GMOS/Gemini spectroscopy of 53 stars in the inner target region were obtained to derive radial velocities and at- mospheric parameters. We also employed photometric and proper motion data. The analysis method consists of the following steps：（i） analysis of the statistical resemblance between the cluster and a set of field samples with respect to the sequences defined in color-magnitude diagrams （CMDs）; （ii） a 5-dimensional iterative exclusion routine was employed to identify outliers from kinematical and posi- tional data; （iii） isochrone fitting to thes× （J - Ks） CMD of the remaining stars and the dispersion of spectral types along empirical sequences in the （J - H） × （H - Ks） diagram were checked. A group of stars was identified for which the mean heliocentric distance is compatible with that obtained via isochrone fitting and whose metallicities are compatible with each other. Fifteen of the member stars observed spectroscopically were identified together with another 19 probable members. Our results indi- cate that NGC 7193 is a genuine OCR, of a once very populous OC, for which the following parameters were derived： d = 501±46 pc, t = 2.5±1.2 Gyr, （[Fe/H]） = -0.17±0.23 and E（B-V） = 0.05±0.05. Its luminosity and mass functions show depletion of low mass stars, confirming the OCR is in a dynam- ically evolved state.

关键词： open cluster remnants - Galactic open clusters

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Morphological and kinetic parameters of the absorption of nitrogen forms for selection of Eucalyptus clones

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Journal of Forestry Research 2021年第4期32卷 1599-1611页

作者： Matheus Severo de souza Kulmann Betania Vahl de Paula Paula Beatriz Sete wagner Squizani Arruda Gabriel Alberto Sans Camila Peligrinotti Tarouco Luciane Almari Tabaldi Fernando Teixeira Nicoloso Gustavo Brunetto Soil Science department Federal University of Santa MariaSanta MariaRS 97105-900Brazil Federal University of Santa Catarina Admar Gonzaga Highway1346FlorianópolisSC 88034-001Brazil Biology department Natural and Exact Sciences CenterFederal University of Santa MariaSanta MariaRS 97105-900Brazil

Eucalyptus clones are selected according to productivity,wood quality,rooting capacity,and resistance to drought,frost and ***,kinetic and morphological parameters that determine the absorption efficiency of nutrients such as nitrate(NO_(3)^(-)) and ammonium(NH_(4)^(+))are often not considered in breeding *** objective of this study was to evaluate the morphological,physiological and kinetic parameters of nitrogen uptake by clones of Eucalyptus saligna(32,864) and Eucalyptus grandis(GPC23).Morphological parameters in shoot and root systems,biomass and N concentrations in different organs,photosynthetic pigment concentrations,parameters of chlorophyll a fluorescence and photosynthetic rates were *** parameters,maximum absorption velocity(V_(max)),Michaelis-Menten constant(K_(m)),minimum concentration(C_(min)) and influx(I) were calculated for NO_(3)^(-)and NH_(4)^(+) in the two *** clone was more efficient in the uptake of NO_(3)^(-)and NH_(4)^(+),and showed lower K_(m) and C_(min)values,allowing for the absorption of nitrogen at low concentrations due to the high affinity of the absorption sites of clone roots to NO_(3)^(-)and NH_(4)^(+).Higher root lengths,area and volume helped the *** clone in absorption efficiency and consequently,resulted in higher root and shoot *** *** clone had higher K_(m) and Cmin for NO_(3)^(-)and NH_(4)^(+),indicating adaptation to environments with higher N *** results of NO_(3)^(-)and NH_(4)^(+) kinetic parameters indicate that they can be used in Eucalyptus clone selection and breeding programs as they can predict the ability of clones to absorb NO_(3)^(-)and NH_(4)^(+) at different concentrations.

关键词： Ammonium and nitrate Eucalyptus saligna Eucalyptus grandis Root system architecture Nitrogen influx Maximum absorption velocity(V_(max)) Michaelis–Menten constant(K_(m)) Minimum concentration(C_(min))

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Techno-functionality of fisetin-enriched yoghurt fermented with Lactobacillus acidophilus bio-capsules produced via osmoporation

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Systems Microbiology and Biomanufacturing 2022年第4期2卷 743-749页

作者： Eduardo wagner Vasconcelos de Andrade Sebastien Dupont Laurent Beney Edilene souza da Silva Roberta Targino Hoskin Márcia Regina da Silva Pedrini Bioprocess Laboratory Chemical Engineering DepartmentUniversidade Federal do Rio Grande do NorteLagoa NovaNatalRN 59078-900Brazil Laboratory of Bioactive Compounds Chemical Engineering DepartmentUniversidade Federal do Rio Grande do NorteLagoa NovaNatalRN 59078-900Brazil UMR Procédés Alimentaires et Microbiologiques(PAM UMR A 02.102)Univ.Bourgogne Franche-ComtéAgroSup Dijon21000 DijonFrance

Osmoporation is a novel encapsulation approach for bioactive compounds based on the osmoresistance mechanisms of microbial *** the best of our knowledge,this is the first study investigating the production of fisetin-enriched yoghurt using Lactobacillus acidophilus-based bio-capsules via osmoporation as the starter *** showed that the milk acidification with fisetin-loaded *** progressed at a slower pace due to complex mechanisms induced by osmopo-ration and internalized *** fermentation using fisetin bio-capsules reached a maximum acidification rate of 0.18 pH units/h after 23 h and pH 4.6 was achieved after 32 ***,the antioxidant activity of yoghurts produced with fisetin bio-capsules did not change during cold storage,while the antioxidant activity of yoghurt produced with non-encapsulated fisetin was reduced by 2.5-fold after 28 ***,this study shows that fisetin osmoporation using *** is a versatile encapsulation bioprocess that enables the delivery of preserved phytoactives into fermented foods like *** strategy has the potential to be extended to other applications in the dairy industry using lactic acid bacteria as both the encapsulation matrix and fermentation agent.

关键词： Cell-based carriers Probiotic bacteria Dairy fermented products Food biotechnology Functional foods

Guy Structure with Galvanic Corrosion： Case Study

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Journal of Civil Engineering and Architecture 2016年第7期10卷 792-797页

作者： Romildo Alves Berenguer Edmilson Raimundo de Oliveira Junior Marcus wagner pereira da Silva Glauber Beirao de souza Paulo Helene Eliana Cristina Barreto Monteiro department of Civil Engineering University of Pernambuco Recife 51110-200 Brazil department of Civil Engineering Catholic University of Pernambuco Reeife 51110-200 Brazil Ph.D.-Engenharia Department of Civil Engineering Street Viscount in Black Gold S6o Paulo 01303-060 Brazil

Reinforcement bar corrosion depends heavily on environment and the processes used. This study will address galvanic corrosion. Based on a case study of galvanic corrosion in a guyed power transmission tower, experiments using a number of methods were made on a small sample of eight poles chosen for excavation and visual inspection, to assess the various resulting diagnoses. Method that provided the best results both in location and classification of the points was adopted, where corrosion had already begun, considering the concern of finding a non-evasive process. To diagnose the status of the poles inserted in rock, after applying the methods, the main types of corrosion affecting them were listed and classified. With the results, the possible means of preventing and maintaining the structure were presented.

关键词： Corrosion diagnosis inspection.

Geostatistical seismic inversion and 3D modelling of metric flow units,porosity and permeability in Brazilian presalt reservoir

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Petroleum Science 2024年第3期21卷 1699-1718页

作者： Rodrigo Penna wagner Moreira Lupinacci Exploratory Interpretation and Reservoir Characterization Group(GIECAR) Department of Geology and GeophysicsUniversidade Federal FluminenseNiteroiRJ24210-346Brazil Petrobras Rio de JaneiroRJ20031-912Brazil

Flow units(FU)rock typing is a common technique for characterizing reservoir flow behavior,producing reliable porosity and permeability estimation even in complex geological ***,the lateral extrapolation of FU away from the well into the whole reservoir grid is commonly a difficult task and using the seismic data as constraints is rarely a subject of *** paper proposes a workflow to generate numerous possible 3D volumes of flow units,porosity and permeability below the seismic resolution limit,respecting the available seismic data at larger *** methodology is used in the Mero Field,a Brazilian presalt carbonate reservoir located in the Santos Basin,who presents a complex and heterogenic geological setting with different sedimentological processes and diagenetic *** generated metric flow units using the conventional core analysis and transposed to the well log ***,given a Markov chain Monte Carlo algorithm,the seismic data and the well log statistics,we simulated acoustic impedance,decametric flow units(DFU),metric flow units(MFU),porosity and permeability volumes in the metric *** aim is to estimate a minimum amount of MFU able to calculate realistic scenarios porosity and permeability scenarios,without losing the seismic lateral *** other words,every porosity and permeability volume simulated produces a synthetic seismic that match the real seismic of the area,even in the metric *** achieved 3D results represent a high-resolution fluid flow reservoir modelling considering the lateral control of the seismic during the process and can be directly incorporated in the dynamic characterization workflow.

关键词： Flowunits Geostatistical inversion Presalt reservoir 3D reservoir modelling Petrophysical modelling

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LC-ESI-IT-MS/MS and MALDI-TOF Approach:Identification of Natural Polymers from Rhizophora mangle Barks and determination of Their Analgesic and Anti-inflammatory Properties

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Natural Products and Bioprospecting 2019年第1期9卷 23-34页

作者： Leonardo Mendes de souza Mesquita Caroline Fabri Bittencourt Rodrigues Claudia Quintino da Rocha Mayara Silveira Bianchim Clenilson Martins Rodrigues Vanda Maria de Oliveira Henrique Hessel Gaeta Mariana Novo Belchor Marcos Hikari Toyama wagner Vilegas Laboratory of Bioprospection of Natural Products(LBPN) UNESP-Sao Paulo State University/Coastal Campus of Sao VicentePc¸a Infante Dom Henrique S/NSao VicenteSao Paulo CEP:11330-900Brazil Laborato´rio de Estudos Avanc¸ados em Fitomedicamentos(LEAF) UFMA-Federal University of MaranhaoAv.dos Portugueses1966-BacangaSao Luı´sMaranhao CEP:65080-805Brazil Embrapa Agroenergy Brazilian Agricultural Research CorporationW3 NortePqEBBrası´liaDF 70770-901Brazil

We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks(AERM)using mass spectrometry analysis[liquid chromatography(LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS(MALDI-TOF)].Analgesic activity was evaluated by formalin and tail-flick experimental ***-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 *** first series of experiments involved[LC]–FIA–IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3(DP3).The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series:one based on polymers of m/z 288 Da increments(up to DP12)and another series based on polymers of m/z[288?162]Da increments(up to DP11).In addition to these series of flavan-3-ol,each DP had a subset of masses with a variation of-16 Da(homologous series of afzelechins-m/z 873–3465 Da)and +16 Da(homologous series of gallocatechins-m/z 905–3497 Da).A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series:glucogallocatechins(m/z 779–3371)and glucoafzelechins(m/z 747–3339).The intraperitoneal administration of different doses of AERM(50,150 and 300 lg mL^(-1))have a morphine-like effect and intense anti-inflammatory activity.

关键词： Catechins Tannins Morphine-likeeffect Anti-inflammatory Mangroves

Comprehensive analysis of HFE gene in hereditary hemochromatosis and in diseases associated with acquired iron overload

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World Journal of Hepatology 2019年第2期11卷 186-198页

作者： wagner Narciso de Campos Juliana Doblas Massaro Eduardo Luiz Rachid Can?ado Cláudia Emília Vieira Wiezel Aguinaldo Luiz Sim?es Andreza Correa Teixeira Fernanda Fernandes de souza Celso Teixeira Mendes-Junior Ana de Lourdes Candolo Martinelli Eduardo Ant?nio Donadi Division of Clinical Immunology Department of MedicineRibeir?o Preto Medical SchoolUniversity of S?o Paulo department of Gastroenterology Clinical Gastroenterology and Clinical Hepatology of Clinical HospitalUniversity of S?o Paulo School of Medicine department of Genetics Ribeir?o Preto Medical SchoolUniversity of S?o Paulo Division of Gastroenterology Department of MedicineRibeir?o Preto Medical SchoolUniversity of S?o Paulo departamento de Química Laboratório de Pesquisas Forenses e Gen?micasFaculdade de FilosofiaCiências e Letras de Ribeir?o PretoUniversidade de S?o Paulo

BACKGROUND Patients with hepatitis C virus(HCV) and hepatocellular carcinoma(HCC) may or not develop iron overload(IO),which is associated with worst prognosis,because can cause serious damage to *** gene controls the iron uptake from gut,particularly in patients with hereditary hemochromatosis(HH).AIM To identify associations between HFE coding region in patients exhibiting hereditary hemochromatosis and in diseases associated with acquired *** We sequenced exons 2 to 5 and boundary introns of HFE gene,evaluating all polymorphic sites in patients presenting hereditary(hemochromatosis) or acquired iron overload HCV and HCC) and in healthy controls,using Sanger *** also determined the ensemble of extended haplotype in healthy control individuals,including several major histocompatibility complex loci,using sequence specific *** reconstruction was performed using the Arlequin and Phase softwares,and linkage disequilibrium(LD) between histocompatibility loci and HFE gene was performed using the Haploview *** The HFE*003 allele was overrepresented(f = 71%) and HFE*001 allele was underrepresented(f = 14%) in HH patients compared to all groups.A strong linkage disequilibrium was observed among the H63 D-G,IVS2(+4)-C and C282 YG gene variants,particularly in HH;however,the mutation IVS2(+4)T>C was not directly associated with HH *** HFE*001/HFE*002 genotype conferred susceptibility to HCC in HCV patients exhibiting IO(P = 0.02,OR =14.14).Although HFE is telomeric to other histocompatibility genes,the H63 DG/IVS2(+4)-C(P ≤ 0.00001/P ≤ 0.0057) combination was in LD with HLA-B*44 allele group in healthy *** LD was observed between HFE alleles and other major histocompatibility *** A differential HFE association was observed for HH and for diseases associated with acquired IO(HCV,HCC).Since HFE is very distant from other histocompatibility loci,only weak associations were observed with these a

关键词： HFE gene Hepatocellular carcinoma Hepatitis C Hemochromatosis hereditary Alleles Haplotypes

Sediment transport under the presence and absence of emergent vegetation in a natural alluvial channel from Brazil

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International Journal of Sediment Research 2016年第4期31卷 360-367页

作者： Yuri Jacques Agra Bezerra da Silva José Ramon Barros Cantalice Vijay P.Singh Cinthia Maria Cordeiro Atanázio Cruz wagner Luís da Silva souza Soil Conservation Engineering Laboratory Rural Federal University of Pernambuco(UFRPE) Brazil department of Biological and Agricultural Engineering Texas A & M University College Station

Studies regarding the influence of emergent vegetation on sediment transport are scarce and have mainly focused on flume *** fill this gap and also meet the international need,we aimed to evaluate the influence of emergent vegetation(Echinodorus macrophyllus) on sediment transport of Capibaribe River,*** and suspended sediment measurements were carried out using the US BLH 84 and US DH 48 samplers,*** of stem diameter,stem spacing and plant density were performed in conjunction with flow and sediment field *** on our results,0.45 m swas the threshold of mean flow velocity supported by *** under field *** value can be helpful for other rivers with gravel-bed river to armoured layer ratio(AR=D)/D=12.50)-natural conditions observed in Capibaribe River-or where the vegetation can provide positive effects,such as increase the bed stability,assist water restoration/rehabilitation and decrease water *** results can hopefully be used in engineering practice and ecosystem *** general,both the drag coefficient and drag force varied inversely and directly with the mean flow velocity and vegetation density,*** vegetation resistance force was inversely proportional to the bedload transport owing to the resistance caused by emergent *** finding was supported by the clear decoupling between nonvegetated and emergent vegetated conditions indicated by cluster *** study results provided a reasonable understanding of the interaction between emergent vegetation,water flow and sediment transport in the Capibaribe River.

关键词： Vegetation drag force Vegetation drag coefficient Plant Reynolds number Hierarchical cluster analysis and principal component analysis

Thermal Shock of Al2O3-SiC Ceramic Material Obtained by Replication Method

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材料科学与工程（中英文版） 2010年第4期4卷 66-70页

作者： Wilson Acchar wagner Lopes Torquato Antonio Carlos Costa Femando Barcelos souza Eduardo Galvao Ramalho Pos-graduation Program of Science and Materials Engineering-UFRN NataI-RN CEP：59072-970 Brazil Pos-graduation Program of Mechanical Engineering-UFRN Natal-RN CEP： 59072-970 Brazil