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characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing

Journal of Integrative Plant Biology 2018年第8期60卷 670-690页

作者： Yuling Zhang Rita Giuliani Youjun Zhang Yang Zhang wagner Luiz Araujo Baichen Wang Peng Liu Qi Sun Asaph cousins Gerald Edwards Alisdair Fernie Thomas P. Brutnell Pinghua Li State Key Laboratory of crop Biology College of Agronomic Sciences Shandong Agricultural University Tai'an 271018 China School of Biological Sciences Molecular Plant Sciences Washington State University Pullman WA 99164-4236 USA Max-Planck-lnsitut for Molekulare Pflanzenphysiologie Am Muhlenberg 1 Potsdam-Golm D-14476 Germany Department of Agronomy and Horticulture University of Nebraska-Lincoln Nebraska USA Photosynthesis Research center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences No. 20 Nanxincun Xiangshan Beijing 100093 China Department of Statistics iowa State University Ames iowa 50011 USA computational Biology Service Unit Life Sciences Core Laboratories Center Cornell University ithaca New York 14850 USA Donald Danforth Plant Science center St. Louis Missouri 63132 USA

In c4 photosynthesis, pyruvate orthophosphate dikinase （PPDK） catalyzes the regeneration of phospho- enolpyruvate in the carbon shuttle pathway. Although the biochemical function of PPDK in maize is well characterized, a genetic analysis of PPDK has not been reported. In this study, we use the maize transposable elements Nlutator and Ds to generate multiple mutant alleles of PPDK. Loss-of- function mutants are seedling lethal, even when plants were grown under 2% cO2, and they show very low capacity for cO2 assimilation, indicating c4 photosynthesis is essential in maize. Using RNA-seq and Gc-MS technologies, we exam- ined the transcriptional and metabolic responses to a deficiency in PPDK activity. These results indicate loss of PPDK results in downregulation of gene expression ofenzymes of the c4 cycle, the calvin cycle, and components of photochemistry. Furthermore, the loss of PPDK did not change Kranz anatomy, indicating that this metabolic defect in the c4 cycle did not impinge on the morphological differentiation of c4 characters. However, sugar metabolism and nitrogen utilization were altered in the mutants. An interaction between light intensity and genotype was also detected from transcriptome profiling, suggesting altered transcriptional and metabolic responses to environmental and endogenous signals in the PPDK mutants.

关键词： characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing

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Approaches and tools for user-driven provenance and data quality information in spatial data infrastructures

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International Journal of Digital Earth 2023年第1期16卷 1510-1529页

作者： Julia Fischer Lukas Egli Juliane Groth caterina Barrasso Steffen Ehrmann Heiko Figgemeier christin Henzen carsten Meyer Ralph Müller-Pfefferkorn Arne Rümmler Michael wagner Lars Bernard Ralf Seppelt Department of computational Landscape Ecology UFZ—Helmholtz Centre for Environmental ResearchLeipzigGermany Macroecology and Society Group German Centre for Integrative Biodiversity Research(iDiv)Halle-Jena-LeipzigLeipzigGermany chair of Geoinformatics Technische Universität DresdenDresdenGermany Institute of Biology Leipzig UniversityLeipzigGermany Institute of Geoscience&Geography Martin Luther University Halle-WittenbergHalle(Saale)Germany center for Information Services and High Performance computing Technische Universität DresdenDresdenGermany

Geospatial data are fundamental in most global-change and sustainability-related ***,readily accessible information on data quality and provenance is often missing or hardly accessible for users due to technical or perceptual barriers,for example,due to unstructured metadata information or missing *** an interdisciplinary process encompassing perspectives of data users,data producers,and software developers,we identified major needs to facilitate effectivefitness-for-use assessments by data users and developed approaches to address *** provided a stylized analysis of large-scale land use data to showcase selected *** support data users,interoperable quality and provenance information need to be meaningfully *** producers need efficient workflows and tools supporting them in creating high-quality,structured and detailed quality and provenance *** newly developed approaches to increase the availability of structured metadata synthesize new and existing tools to extract metadata or to generate provenance data during *** our approaches to improve interoperability and accessibility we present novel tools to support(i)the creation of curated and linked registers of data quality indicators and thematic terms,and(ii)linked visualization of data quality and provenance *** our approaches increases transparency,facilitatesfitness-for-use assessments,and ultimately improves research quality.

关键词： Dashboard earthsystem science fitness forpurpose ISO 19157 landuseandland cover data ontology

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声学的大涡模拟

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国外科技新书评介 2008年第12期 10-11页

作者： c．wagner（编）吴永礼不详中国科学院力学研究所研究员

本书是《剑桥航空和航天》系列丛书第20卷。由于飞机、火车和工业造成的噪声污染引起极大的关注，工程师和研究人员着力于把大涡模拟（LES）用于减少工业噪音的设计和声学研究。本书由30多位专家撰写，由3位博士编辑而成，书中介绍声学... 详细信息

本书是《剑桥航空和航天》系列丛书第20卷。由于飞机、火车和工业造成的噪声污染引起极大的关注，工程师和研究人员着力于把大涡模拟（LES）用于减少工业噪音的设计和声学研究。本书由30多位专家撰写，由3位博士编辑而成，书中介绍声学和大涡模拟的理论基础以及相关的数值方法，详细讨论了用于声源预测的雷诺平均纳维一斯托克斯（RANS）／大涡模拟杂交技术，给出了一些应用实例，包括混合层和喷气流动的简单几何到复杂的机翼和汽车几何，虽然现在对于所有重要应用问题还没有完美的求解方法，但是，书中给出了当前的方法、

关键词：大涡模拟声学噪声污染研究人员数值方法杂交技术雷诺平均求解方法

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基于场景的软构件自动化测试模型研究

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湘潭大学自然科学学报 2009年第1期31卷 141-147页

作者：陈翔 Michael wagner 肖杰邓毅刚长沙学院计算机科学与技术系湖南长沙410003 儿童医院信息技术中心美国俄亥俄州辛西那提市oh45220 长沙学院计算机教学中心湖南长沙410003 长沙威胜电子有限公司湖南长沙410013

随着软构件技术的快速发展,聚集软构件设计复杂软件系统的软件开发方法日趋成熟.如何利用系统架构和软构件的可靠性分析软件系统的可靠性成为一个亟待解决的问题.提出了一种基于场景的软构件自动化测试模型,给出基于统一建模语言UML的... 详细信息

随着软构件技术的快速发展,聚集软构件设计复杂软件系统的软件开发方法日趋成熟.如何利用系统架构和软构件的可靠性分析软件系统的可靠性成为一个亟待解决的问题.提出了一种基于场景的软构件自动化测试模型,给出基于统一建模语言UML的顺序图构造测试场景具体方法,并对基于UML顺序图构造测试场景方法的有效性进行了分析.

关键词：软构件可靠性测试场景测试用例工作流拦截器面向方面技术

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Suppression of the External MitochondrialNADPH Dehydrogenase, NDB1, in Arabidopsisthaliana Affects central Metabolism andVegetative Growth

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Molecular Plant 2014年第2期7卷 356-368页

作者： Sabá V. Wallström Igor Florez-Sarasa wagner L. Araújo Mari Aidemark María Fernández-Fernández Alisdair R. Fernie Miquel Ribas-carbó Allan G. Rasmusson Department of Biology Lund University Biology building Sölvegatan 35 SE-223 62 Lund Sweden Grup de Recerca en Biologia de les Plantes en condicions Mediterrànies Universitat de les Illes Balears Ctra Valldemossa Km. 75 07122 Palma de Mallorca Spain Present address: Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 1 14476 Potsdam-Golm Germany Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 1 14476 Potsdam-Golm Germany Present address: Departamento de Biologia Vegetal Universidade Federal de Viçosa 36570-000 Viçosa Minas Gerais Brasil

ca2＋-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPHdehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript wassuppressed by 80% in Arabidopsis thaliana plants, and external ca2＋-dependent NADPH dehydrogenase activity becameundetectable in isolated mitochondria. This was linked to a decreased level of NADP＋ in rosettes of the transgenic ***-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of 14c-glucose was increased. On soil, NDBl-suppressing plants had a decreased vegetative biomass, but leaf maximumquantum efficiency of photosystem Ⅱ and cO2 assimilation rates, as well as total respiration, were similar to the wild-type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealeddecreased levels of sugars, citric acid cycle intermediates, and amino acids in the transgenic lines. The NDBl-suppressioninduced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. Thetranscriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thalianaoverexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP（H）reduction levels, which in turn affect central metabolism and growth, and interact with defense signaling.

关键词： citric acid cycle metabolic profiling mitochondrial respiration NADPH RNA interference type II NAD(P)Hdehydrogenase vegetative growth

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Mechanical behavior of iron ore tailings under standard compression and extension triaxial stress paths

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第7期15卷 1883-1894页

作者： Alexia cindy wagner João Paulo de Sousa Silva João Vítor de Azambuja carvalho Ana Luisa cezar Rissoli Pedro Pazzoto cacciari Helder Mansur chaves Hugo carlos Scheuermann Filho Nilo cesar consoli Universidade Federal do Rio Grande do Sul Porto Alegre90035-190Brazil Mineral Development centre Vale S.A.Santa Luzia33040-900Brazil Department of civil Geological and Mining EngineeringPolytechnique MontrealMontrealH3C 3A7Canada

The disposal of mining tailings has increasingly focused on the use of dry *** structures offer more security since they use filtered and compacted *** of the construction method and the heights achieved,the material that compounds the structure can be subjected to different stress paths along the failure *** theoretical framework considered in the design of these structures generally is the critical state soil mechanics(cSSM).However,the data in the literature concerning the uniqueness of critical state line(cSL)is still controversial as the soil is subjected to different stress *** respect to tailings,this question is even more *** paper studies two tailings with different gradings due to the beneficial processes over extension and compression paths.A series of drained and undrained triaxial tests was conducted over a range of initial densities and stress *** the q-p'plane,different critical stress ratio(M)values were obtained for compression and extension stress ***,the critical state friction angle is very similar with a slightly higher critical state friction angle for extension *** stress path dependent cSLs were obtained in the n-lnp0 plane with the extension tests below the cSL defined in *** the fines content,the studied tailings presented very similar M and critical state friction angle ***,the fines content af-fects the volumetric behavior of the studied tailings and the cSLs on the n-lnp0 plane shift downwards with the increasing fines content for compression and extension *** relation to dilatancy analysis,the fines content did not present an evident influence on the dilatancy of the ***,different values of mean stress ratio N were obtained between compression and extension tests and can corroborate the existence of non-unique cSLs for these materials.

关键词： Iron ore tailings(IOTs) Dry stacking critical state soil mechanics Extension tests Stress path

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Synthesis, characterization and Analysis of Leishmanicide Ability of the compound [Ru(cl)3(H2O)2(gly)]

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Open Journal of Inorganic chemistry 2017年第4期7卷 89-101页

作者： Marcio Adriano S. chagas Anderson Dourado Galvã o Fabricio Tarso de Moraes A. T. B. N. Ribeiro Adriano Buzutti de Siqueira I. c. c. de Assis Salama wagner Welber Arrais-Silva Katia Meirelles Duarte de Sousa claudia cristina de Sousa Pereira wagner Batista dos Santos Federal University of Mato Grosso Barra do Gar&ccedil as Brazil Federal University of Sergipe S&atilde o cristovã o Brazil

Studies of coordinated compounds containing ruthenium (Ru2+ and Ru3+) have shown very effective in vitro results for the treatment of cancer and neglected diseases such as leishmaniasis. In this paper, we present the synthesis of the compound [Ru(cl)3(H2O)2(gly)], which was characterized by spectroscopic (Ultraviolet-visibleand infrared) and thermal analysis (Thermogravimetry/Derived Thermogravimetry and Thermogravimetry/Differential Thermal Analysis). The analysis of the compound in the Ultraviolet-visibleregion showed a 290 nm band λmax (ε= 1.685 × 103 L·cm-1·mol-1), attributed to the ligand metal charge transfer (LMcT). The spectroscopy (IR) showed major vibrational bands at δa (-cOO-) 1664 cm-1, δs (-cOO-) 1388 cm-1, δs () 1571 cm-1 and δs (ccN) 889 cm-1. The thermal analysis by TG/DTG and TG-DTA indicated that the complex has five consecutive stages of decomposition: at 115°c (TG = 12.18%;calculated = 11.32%) H2O (coordinating water), exothermic peaks at 230°c, 307°c, 440°c and 463°c due to oxidative decomposition of glycine, followed by the formation of RuclO residue at 665°c (TG = 41.11%;calculated = 40.81%). The thermal characterization suggested the stoichiometry of the complex [Rucl3(H2O)2(gly)]. The antileishmanial capacity of this compound was also evaluated and the results indicated a 31% decrease in the parasitic infection of macrophages and a 1.5 to 3 fold reduction in the number of parasites per cell after treatment with 100 μg/mL of the complex. These results support the possible use of this compound as a therapeutic alternative against medical and veterinary parasites.

关键词： Ruthenium Glycine Spectroscopy Infrared TG/DTG and TG/DTA Amino Acid

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Productive Performance of Wheat Based on Nitrogen Fertilization in coverage

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American Journal of Plant Sciences 2015年第9期6卷 1587-1593页

作者： Michael Jonathan Fernandes Alves Wilson wagner Ribeiro Teixeira Edelclaiton Daros Luís césar cassol Joao Augusto Lopes Pascoalino Milton Ferreira de Moraes Department of Plant Science and crop Protection Federal University of ParanaCuritibaBrazil Department of Soil Science Federal University of ParanáCuritibaBrazil University of ParanáFederal Technology Pato BrancoBrazil

The objective of this work was to evaluate genotypes of wheat depending on doses of N in order to determine the best value of N and the components of productivity of wheat. Experiment was carried out in Pato Branco—PR in soil classified as Eutrophic typical RED LATOSOL. The experimental design was randomized blocks, in 3 × 5 (× N doses genotypes), with three repetitions. The genotypes were: cD 108, cD 115 and BRS 220, and the doses of n were: zero;20;40;80 and 120 kg of N·ha-1, applied at the beginning of tillering. After harvest assessed number of Spike (NE), Tang (Ec) length, grains per Spike (GE), mass of thousand grains (MMG), grain yield (R) and weight/HL (PH), application of N increased the NE and R, and however decreased to MMG. The genotypes differed among themselves, featuring better performance of the NE (the BRS 220), cE (the cD 115) and R (the cD 108). There were positive correlations between the doses of N with the NE and R and be-tween NE with the R, and negative correlation between doses of N with the MMG. It is concluded that the NE is the main factor that affects the R with N supply.

关键词： Triticum aestivum Grain Yield Nitrogen

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Impact of cytomegalovirus reactivation just before liver transplantation:A prospective cohort study

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World Journal of Gastrointestinal Pathophysiology 2021年第3期12卷 51-58页

作者： claudio Marcel B Stadnik cassia Ferreira B caurio Edison M Rodrigues-Filho wagner L Nedel Guido Pc cantisani Maria L Zanotelli Alessandro c Pasqualotto Infection control Department Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Molecular Biology Laboratory Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Intensive care Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Liver Transplant Unit Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Infectious Diseases Unit Universidade Federal de Ciencias da Saude de Porto AlegrePorto Alegre 90035075RSBrazil

BAcKGROUND cytomegalovirus(cMV)is the most common viral pathogen after liver transplantation(LT).Although reactivation of cMV infection is generally described in the context of immunosuppression,it has also been described in critically ill immunocompetent patients including cirrhotic *** To determine the incidence of reactivated cMV prior to *** This was a prospective cohort study evaluating adult patients who underwent LT between 2014 and 2016.A plasma sample was obtained from all patients for cMV quantitative real-time PcR testing right before *** were followed for at least 1 year to assess the following outcomes:Incidence of cMV infection,organ rejection and overall *** A total of 72 patients were *** patients died before transplantation,thus 68 patients were followed up for a median of 44 mo(20-50 mo).In 23/72 patients(31.9%)cMV was reactivated before ***-transplantation,16/68(23.5%)patients had cMV infection and that was significantly associated with the recipient being cMV negative and a cMV-positive *** cMV reactivation was not associated with overall mortality(log rank:0.9).cONcLUSION This study shows that cMV infection is common in patients with chronic liver disease just before LT,but the clinical impact of this infection seems to be negligible.

关键词： Liver transplantation cytomegalovirus infection Quantitative real-time PcR Risk factors Liver cirrhosis Molecular biology

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超高周疲劳中碳锰合金的金属学不稳定性影响

超高周疲劳中碳锰合金的金属学不稳定性影响

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中国力学学会学术大会'2009

作者：黄志勇 wagner D Bathias c 能源与动力学院南京航空航天大学 LEEE Unerversite Paris XNanterre 92410France

在超高周疲劳试验中,碳锰合金钢的热耗散表现出于其他材料不同的行为。碳锰钢中,位错的变动性受到间质原子作用,而间质原子的影响取决于应变率和晶粒尺寸。已观察到的单调载荷下的几种不稳定性金属学表现为:

关键词：超高周疲劳热耗散 L(u|¨)ders带

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