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American Journal of Analytical chemistry 2012年第2期3卷 138-146页

作者： claudia Andrea Lima cardoso Fabiana Volpe Zanutto Eliana Aparecida Varanda Paulo Takeo Sano wagner Vilegas Lourdes campaner dos Santos UEMS Course of ChemistryState University of Mato Grosso do SulDouradosMato Grosso do SulBrazil Department of Biological Sciences Faculty of Pharmaceutical SciencesUNESP-Universidade Estadual PaulistaAraraquaraSao PauloBrazil Institute of Biosciences Department of BotanyUniversity of Sao PauloSao PauloBrazil campus Experimental do Litoral Paulista UNESP-Universidade Estadual PaulistaSao VicenteSao PauloBrazil Institute of chemistry Department of Organic ChemistryUNESP-Universidade Estadual PaulistaAraraquaraSao PauloBrazil

The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in methanolic aerial-part extracts of Paepalanthus giganteus and Syngontnhus nitens from the Eriocaulaceae family. Their small capitulae hinder morphological analysis, and thus complicate taxonomic studies of these species, which present anti-ulcer, antimutagenic and antioxidant activities. Taxonomic studies of these plants revealed that the Paepalanthus genus presents flavonols and naphthopyranones while the Syngontnhus genus has flavone and xanthone as majority compounds. The prepared samples were analyzed quantitatively by High Performance Liquid chromatography with PDA detection for the presence of quercetin, luteolin, 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and paepalantine. The substances were recovered from these samples at rates from 98.01 to 99.99%. The coefficient of variation in the quantitative analysis of the sample com- pounds was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the sam- ples with re-spective determination coefficients of r2 = 0.9999 (luteolin and 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone) and r2 = 0.9998 (quercetin and paepalantine) for the linear range of the analytical calibration curves of the samples. The detection limits were 0.07 μg?mL–1 for quercetin and luteolin, 0.06 μg?mL–1 for 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and 0.10 μg?mL–1 for paepalantine. The quantification limits were 0.23 μg?mL–1 for quercetin and luteolin, 0.20 μg?mL–1 for 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and 0.33 μg?mL–1 for paepalantine by Lc. The method was considered sen- sitive for quantification of the quercetin, luteolin, 3,6-dimethoxy-1,5,7-tri-hydroxyxanthone and paepalantine in plant samples.

关键词： Lc-PDA Naphthopyranone Flavonoid Xanthone

Antinecrotic and Antiapoptotic Effects of Different concentrations of copaiferalangsdorffii Essential Oil in Macrophages and Lymphocytes of Mice

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Journal of Agricultural Science and Technology(A) 2013年第8期3卷 659-665页

作者： Marcelo Bettega Lucas wagner Gortz Luiza Fernanda Schier Aniele cristine Ott clemente Osvaldo Malafaia claudia do carmo consuelo Ota Department of Medical Research Faculdade Evangdlicado Parana Evangelical University of Paran6 Curitiba Paran6 80240050Brazil

Purpose： to evaluate the in vitro influence of copaiferalangsdorffii essential oil on the antinecrotic activity in macrophages, and on the antiapoptotic activity in macrophages and lymphocytes of Swiss mice. Methods： lymphocytes and macrophages were obtained from ten 60-day-old male mice weighing 42-45 g, and then cultured in 24-well plates. cells were treated with concentrations of 2%, 5%, 10% and 20% of oil from copaiferalangsdorffii before or after induction of apoptosis with ultraviolet light. We used positive and negative controls. Hoechst staining was performed, and then images of cells under fluorescence microscope were captured and digitalized. We used Image J_1.46o to quantify the fluorescence intensity/brightness of condensation of the chromatin ofapoptotic cells. We used one-way ANOVA with Bonferroni＇s and Tukey＇s multiple comparison tests. Results are expressed as the means of relative densities after adjustment and 95% confidence intervals; P 〈 0.05 was considered statistically significant. Results： oil from copaiferalangsdorffii at different concentrations has decreased necrosis and apoptosis of both lymphocytes and macrophages from mice, when compared to c＋（all P 〈 0.05）. Discussion： copaiferalangsdorffii is typically Brazilian and found in the Amazon region, with anti-inflammatory, antioxidant and antineoplastic effects due to its oil compounds, such as xyloglucans and kaurenoic acid. Its potential therapeutical usage was demonstrated in a national study. The present study showed that its oil has significant in vitro antiapoptotic and antinecrotic activity and it should be tested experimentally in vivo.

关键词： Necrosis apoptosis tropical tree species phytopharmacology.

用f-k技术分析NORESS台阵和单台资料以识别近台和近源的散射源

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世界地震译丛 2000年第3期31卷 3-13页

作者： I.N.Gupta c.S.Lynnes T.W.McElfresh R.A.wagner 黄金莉中国地震局分析预报中心

对高频NORESS台阵接收的人工爆破和天然地震的短周期记录进行f-k分析的结果表明,续至波来自于接近台阵西南约25～30km处。应用从台阵记录中减去波束形成而得到的残差地震记录,改进了识别相同散射的效果。将针对后面波至的窗口与针对初动... 详细信息

对高频NORESS台阵接收的人工爆破和天然地震的短周期记录进行f-k分析的结果表明,续至波来自于接近台阵西南约25～30km处。应用从台阵记录中减去波束形成而得到的残差地震记录,改进了识别相同散射的效果。将针对后面波至的窗口与针对初动P波窗口相对于慢度值的f-k功率相减(归一化以后)而得到的f-k功率差图,提供了几乎相同的结果。续至波源好象是短周期的面波Rg,它是由台阵西南27km处的米约萨湖区域入射P波的散射而产生的。该区域从潮的底部到与它相邻的山顶有大约1km的地形起伏。对NORESS三分量台站记录的一次人工爆破的偏振分析也给出了具有Rg偏振特征的二次震相,且波至时间和方位角与f-k分析所得的结果是一致的。通过互换震源和台阵的位置形成互易台阵,用这种台阵资料的f-k分析的结果来研究近源散射。用密间隔的亚卡平地(NTS)炮点在几个台站记录的资料,提供了亚卡峡谷的Rg到P波的散射发生在其近西南部和其他盆地边缘的证据。看来,近接收器P→Rg散射和近震源Rg→P散射的位置能用f-k技术确定。

关键词： f-k技术 NORESS台阵散射源工程地震天然地震

The regulatory effect of pyruvate orthophosphate dikinase (PPDK) on the maize c4 photosynthesis

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The regulatory effect of pyruvate orthophosphate dikinase (P...

On Geodynamic Evolution of Simao Region (Southwestern Yunnan, china) during Late Paleozoic and Triassic

中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Pinghua Li Yanyan Liu Fuquan Gong Rita Giuliani wagner L.Araujo Lauren Dedow Thomas P.Brutnell Institute of Tropical Biosciences & Biotechnology Chinese Academy of Tropical Agriculture Sciences college of Plant Science & technology Huazhong Agriculture University college of Agriculture Hainan University School of Biological Sciences Molecular Plant SciencesWashington State University Max-Planck-lnstitute of Molecular Plant Physiology Boyce Thompson Institute Cornell University

c4 plants,like maize,display a higher photosynthetic efficiency,lower cO compensation point and reduced photorespiration relative to c plants under hot,dry conditions These benefits provide c crops with a distinct growth advantage,better water use efficiency and higher rates of biomass *** important aim of our current research is gain a deeper understanding of the mechanisms that regulate c *** application of this work would be to engineer c crops with c traits thus improving *** orthophosphate dikinase(PPDK) is a key and rate-limiting enzyme in maize c photosynthesis,which catalyzes the regeneration of the substrate phosphoenolpyruvate(PEP) for assimilation of cO by PEP *** the biochemical function of PPDK is well understood,little is known of the transcriptional network regulating *** this study,we characterized several transposon insertions in the maize PPDK *** detailed histological,physiological and molecular analyses(RNA-seq,Gc-MS) we explore the PPDK regulatory network and examine the physiological consequences of a loss of PPDK function.

关键词： Pyurvate orthophosphate dikinase maize c4 photosynthesis RNA-seq

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Journal of china University of Geosciences 2001年第3期12卷 195-200页

作者： Helmcke DGZG, University of Goettingen, D 37077 Goettingen, GermanyIngavat Helmcke Rc/o GZG, University of Goettingen, D 37077 Goettingen, GermanyFeng QinglaiFaculty of Earth Sciences, china University of Geosciences, Wuhan 430074 wagner B Heppe KGZG, GZG University of Goettingen D-37077 Goettingen Germany GZc University of Goettingen D-37077 Goettingen Germany c/o GZG University of Goettingen D-37077 Goettingen Germany Faculty of Earth Sciences China University of Geosciences Wuhan 430074

Over the last years, the Simao region, southwestern Yunnan, china, turned out to be a very promising target to elucidate plate tectonic processes around the Permian Triassic boundary within the Tethyan domain of Asia. New data from this area reveal that Upper Paleozoic compressional deformations occurred along the Lancangjiang and in areas to the east. Along Lancangjiang, an angular unconformity is exposed, which separates quartz phyllites—formed during a carboniferous tectono metamorphic event—from Triassic red beds to roofing rhyolites. The acidic volcanics were often said to be remnants of a volcanic arc that was active during the Triassic subduction and closure of an oceanic realm along the Lancangjiang zone. According to our new data, however, these volcanics indicate most probably an Upper Triassic stage of rifting. In the Yunxian anticline (NW of Simao), an angular unconformity of intra Permian age is exposed where carboniferous to lower Middle Permian strata, which were deposited in a rather deep basin, are unconformably overlain by a shallow marine sequence of upper Middle to Upper Permian sediments. The angular unconformity is of the same age as the syn orogenous sediments first described and dated from the Phetchabun region in Thailand and hence a convincing argument for a Late Variscan orogeny forming an extensive zone of mountain building that can be traced through the central parts of mainland Southeast Asia.

关键词： Simao region Late Paleozoic Triassic tectonics.

传统DNA疫苗载体与Semliki森林病毒复制子对HIV-1 Pr55^(gag)表达与体液免疫原性的比较性研究

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病毒学报 2002年第1期18卷 1-8页

作者：张健慧 Jens Wild Kurt Bieler Marcus Graf Ludwig Deml Hans Wolf PeterLiljestrm Ralf wagner 邵一鸣卫生部艾滋病预防与控制中心参比实验室北京100050 德国雷根斯堡大学微生物研究所瑞典卡洛琳斯卡研究所微生物与肿瘤生物学中心

为探索Semliki森林病毒 (SFV)衍生的复制型DNA载体可否用于HIV疫苗的候选载体 ,对该载体与传统DNA疫苗载体对HIV - 1Pr5 5 gag的表达与体液免疫原性进行了系统比较研究。将野生型 (wtgag)及密码子改造(syngag)的HIV - 1ⅢBgag 基因分别... 详细信息

为探索Semliki森林病毒 (SFV)衍生的复制型DNA载体可否用于HIV疫苗的候选载体 ,对该载体与传统DNA疫苗载体对HIV - 1Pr5 5 gag的表达与体液免疫原性进行了系统比较研究。将野生型 (wtgag)及密码子改造(syngag)的HIV - 1ⅢBgag 基因分别克隆于SFVDNA载体及传统DNA疫苗载体 [pcDNA3 1(+) ],对其Pr5 5 gag细胞内表达水平、Pr5 5 gag病毒样颗粒释放、以及在BALB/c鼠的体液免疫原性进行了比较。在 2 93T、H12 99、c2c12和BHK细胞系中 ,SFV -wtgag可以Rev非依赖方式有效表达Pr5 5 gag ,而 pc -wtgag转染的细胞不能有效表达Pr5 5 gag,从而不能诱导小鼠产生免疫反应。虽然SFV质粒的细胞转化效率明显低于pcDNA载体 ,SFV -wtgag和SFV -syngag在细胞内Pr5 5 gag的表达量与 pc -syngag相似 ,而Pr5 5 gag病毒样颗粒的释放明显低于 pc -syngag。在肌内注射免疫的小鼠中 ,低剂量 (0 1和 1 0 μg)的SFV及 pcDNAgag表达质粒均未诱导出GAG特异性免疫反应。在高剂量 (10 ,30 ,10 0 μg)免疫组中 ,与SFVgag表达质粒相比 ,pc -syngag可诱导出较高水平的TH1型GAG特异性抗体。SFV -syngag较SFV -wtgag可诱导出高水平的体液免疫反应。结果提示 ,SFV衍生的复制子单独使用不能在小鼠诱导出优于传统DNA疫苗载体的HIV - 1GAG特异性?

关键词： HIV-1 Semliki森林病毒 DNA疫苗表达免疫原性载体复制子

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桑给巴尔岛附近硬珊瑚的分布和丰度模式

AMBIO－人类环境杂志 2002年第7期31卷 609-611页

作者：林宝法 Nsajigwa E.Mbije Gregory M.wagner Julius Francis Marcus c.hman Kajsa Garpe National Museum of Tanzania Department of Zoology and Marine Biology University of es Salaam WIOMSA Department of Zoology Stockholm University

东非珊瑚礁的特点是它位于一个离散的动物地理学分区[1],具有经由赤道流接纳和重新分配偏远的种属物质的潜在重要性[2].尽管如此,该地区仍然是世界上研究最少的地区之一.例如,在坦桑尼亚,尽管珊瑚礁是众多的人赖以为生的重要自然资源,... 详细信息

东非珊瑚礁的特点是它位于一个离散的动物地理学分区[1],具有经由赤道流接纳和重新分配偏远的种属物质的潜在重要性[2].尽管如此,该地区仍然是世界上研究最少的地区之一.例如,在坦桑尼亚,尽管珊瑚礁是众多的人赖以为生的重要自然资源,却没有受到应有的重视和研究[3～5].为数不多的研究定量分析过坦桑尼亚的珊瑚礁结构[6～13],而且大部分只是限于对一些礁砰进行基线研究.

关键词：桑给巴尔岛硬珊瑚丰度模式珊瑚礁群落结构物种分布

Synthesis, characterization of Ruthenium compounds and Studies of Biological Effects in McF-7 Tumors cell Lines

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Advances in Biological chemistry 2022年第3期12卷 61-71页

作者： Andressa Teixeira de Barros Nunes Ribeiro claudia cristina Sousa Adenilda cristina Honório França Eduardo Luzia França Fabricio Tarso Moraes wagner Batista Santos Institute of Exact Sciences and Earth Federal University of Mato Grosso Barra do Gar&ccedil as/MT Brazil Institute of Biological and Health Sciences Federal University of Mato Grosso Barra do Gar&ccedil as/MT Brazil

This work presents the synthesis and characterization of compounds derived from the ruthenium transition metal with the nitrogenous ligand 4-aminopy- ridine (4-ampy). The synthesized compounds were characterized by FTIRmed spectroscopy and TG-DTA thermal analysis. For the cytotoxic evaluation of ruthenium compounds, a 66.0 μM aqueous solution containing the complex and the study of data observed in the biological assessment was performed using variance (ANOVA) analysis, followed by Tukey’s multiple comparisons test. Differences between treatments were considered significant when the p-value was less than 0.05 (p c thermal analysis for the first complex suggests a stoichiometry of [Ru(cl)3(4-ampy)(H2O)2]·1/2H2O, which, due to the low solubility in an aqueous medium, was modified to increase its solubility for biological tests. The analysis of the spectra in the medium infrared region (FTIR) for the complex [Ru(cl)3(4-ampy)(H2O)2]·1/2H2O, shows displacements of the bands observed at 1625 - 1566 cm﹣1 ν(c=c) e (c=N), indicating that coordination to the metallic center occurred by this group. Band displacements were observed in the modified Ru (III) complex, which suggests the presence of the 4-ampy ligand and the coordination by the groups ν(c=c) and (c=N) after the modification. In recent years, researchers worldwide have concentrated on obtaining, developing, and modifying drugs used as chemotherapeutic agents. The evaluation of the cell viability of the modified Ru (III) compound demonstrated cytotoxic effects in the McF-7 cell line (15.33% ± DP 2.7) but did not affect normal cells (PBMc), which reflects the potential for possible applications.

关键词： Ruthenium McF-7 cells cytotoxic Evaluation N-Heterocyclic Ligands

Notes for genera:basal clades of Fungi(including Aphelidiomycota,Basidiobolomycota,Blastocladiomycota,calcarisporiellomycota,caulochytriomycota,chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomycota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and Zoopagomycota)

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Fungal Diversity 2018年第5期92卷 43-129页

作者： Nalin N.Wijayawardene Julia Pawłowska Peter M.Letcher Paul M.Kirk Richard A.Humber Arthur Schüßler Marta Wrzosek Anna Muszewska Alicja Okrasińska Łukasz Istel Aleksandra Gęsiorska Paul Mungai Adebola Azeez Lateef Kunhiraman c.Rajeshkumar Rajshree V.Singh Renate Radek Grit Walther Lysett wagner christopher Walker D.Siril A.Wijesundara Moslem Papizadeh Somayeh Dolatabadi Belle D.Shenoy Yuri S.Tokarev Saisamorn Lumyong Kevin D.Hyde Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China center of Excellence in Fungal Research and School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Molecular Phylogenetics and Evolution Institute of BotanyFaculty of BiologyBiological and Chemical Research CenterUniversity of Warsawul.Zwirki i Wigury 10102-089 WarsawPoland Department of Biological Sciences The University of AlabamaTuscaloosaAL 35487USA Royal Botanic Gardens KewRichmondSurrey TW93DSUK USDA-ARS Emerging Pests and Pathogens Research Robert W.Holley Center for Agriculture and HealthTower RoadIthacaNY 14853-2901USA Symbiosis and Plant-Microbe Association Research Laboratory SYMPLANTA GmbH&Co.KG64287 DarmstadtGermany Institute of Biochemistry and Biophysics Polish Academy of SciencesPawinskiego 5A02-106 WarsawPoland Ecological Monitoring Unit Biodiversity Research and Monitoring DivisionKenya Wildlife ServiceP.O.Box 40241Nairobi 00100Kenya Department of Plant Biology Faculty of Life SciencesUniversity of IlorinIlorinNigeria National Fungal culture collection of India(NFccI) Biodiversity and Palaeobiology(Fungi)GroupAgharkar Research InstitutePuneMaharashtra 411004India Evolutionary Biology Institute for Biology/ZoologyFreie Universität Berlin14195 BerlinGermany National Reference center for Invasive Mycoses(NRZMyk) Leibniz Institute for Natural Product Research and Infection Biology–Hans Knoell InstituteJenaGermany School of Agriculture and Environment Faculty of ScienceUniversity of Western Australia35 Stirling HighwayCrawleyWA 6009Australia Royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK National Institute of Fundamental Studies Hantane RoadKandySri Lanka Department of Microbiology Pasteur Institute of IranTehranIran Faculty of Engineering Sabzevar University of New TechnologySabzevarIran cSIR-National Institute of Oceanography Regional

compared to the higher fungi(Dikarya),taxonomic and evolutionary studies on the basal clades of fungi are fewer in ***,the generic boundaries and higher ranks in the basal clades of fungi are poorly *** DNA based taxonomic studies have provided reliable and accurate *** is therefore necessary to compile all available information since basal clades genera lack updated checklists or ***,Tedersoo et al.(MycoKeys 13:1–20,2016)accepted Aphelidiomycota and Rozellomycota in Fungal ***,we regard both these phyla as members in Kingdom *** accept 16 phyla in basal clades ***,Basidiobolomycota,Blastocladiomycota,calcarisporiellomycota,caulochytriomycota,chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomy-cota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and ***,611 genera in 153 families,43 orders and 18 classes are provided with details of classification,synonyms,life modes,distribution,recent literature and genomic ***,catenariaceae couch is proposed to be conserved,cladochytriales Mozl.-*** emended and the family Nephridiophagaceae is introduced.

关键词： Invalid genera Nephridiophagaceae fam.nov. Outline Phylogeny Validation