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Synthesis and Antimicrobial Activity of New 2,5-Disubstituted 1,3,4-Oxadiazoles and 1,2,4-triazoles and their Sugar.Derivatives

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Chinese Journal of Chemistry 2012年第1期30卷 77-83页

作者： ei-Sayed, wael A. Ali, Omar M Hendy, Hend A Abdei-Rahman, Adel A.-H.t Department of Photochemistry National Research Centre Cairo Egypt Chemistry Department Northern Border University Afar Saudi Arabia Department of Chemistry Faculty of Science Menoufia University Shebin El-Koam Egypt

A series of new 2,5-disubstituted-l,3,4-oxadiazole and 1,2,4-triazole derivatives were synthesized by hetero- cyclization of acid hydrazide I and thiosemicarbazide derivative 2. Furthermore, the acyclic C-nucleoside analogs were prepared by cyclization of their corresponding sugar.hydrazones by reaction with acetic anhydride. the antimicrobial activity of the prepared compounds was evaluated and some of the synthesized compounds revealed good activities against fungi.

关键词： 1,3,4-oxadiazoles 1,2,4-triazoles sugar.hydrazones acyclic nucleosides antimicrobial activity

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Antibody to El peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro

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World Journal of Gastroenterology 2006年第16期12卷 2530-2535页

作者： Mostafa K EL-Awady Ashraf A tabll Khaled Atef Samar S Yousef Moataza H Omran Yasmin ei-Abd Noha G Bader-Eldin Ahmad M Salem Samir F Zohny wael t ei-gar. Department of Biomedical technology National Research CenterCairoEgypt Department of Biochemistry Faculty of ScienceAin Shams UniversityCairoEgypt

AIM： to analyze the neutralizing activity of antibodies against E1 region of hepatitis C virus （HCV）. Specific polydonal antibody was raised via immunization of New Zealand rabbits with a synthetic peptide that had been derived from the E1 region of HCV and was shown to be highly conserved among HCV published genotypes. MEtHODS： Hyper-immune HCV E1 antibodies were incubated over night at 4 ℃ with serum samples positive for HCV RNA, with viral loads ranging from 615 to 3.2 million IU/mL. treated sera were incubated with HepG2 cells for 90 min. Blocking of viral binding and entry into cells by anti E1 antibody were tested by means of RtPCR and flow cytometry. RESULtS： Direct immunostaining using FItC conjugated E1 antibody followed by Flow cytometric analysis showed reduced mean fluorescence intensity in samples pre-incubated with E1 antibody compared with untreated samples. Furthermore, 13 out of 18 positive sera （72%） showed complete inhibition of infectivity as detected by Rt-PCR. CONCLUSION： In house produced E1 antibody, blocks binding and entry of HCV virion infection to target cells suggesting the involvement of this epitope in virus binding and entry. Isolation of these antibodies that block virus attachment to human cells are useful as therapeutic reagents.

关键词： Flow cytometry Hepatitis C virus E1 envelope therapeutic antibodies Direct immuno-fluorescence HepG2 cells

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