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Assessment of spermatogenesis in camels via seminiferous tubule staging and testosterone profiling

Asian pacific Journal of Reproduction 2024年第3期13卷 133-142页

作者： Saif Ullah Abdul Quddus Quanwei Wei wael ennab Sheeraz Mustafa Jiahao Sun Rahmani Mohammad Malyar Abdul Baseer Dagan Mao Fangxiong Shi College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China Faculty of Veterinary and Animal Sciences Lasbela University of Agriculture Water and Marine SciencesBalochistanPakistan Faculty of Veterinary and Animal Sciences Ziaddin University Karachi Pakistan

Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel ***:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local *** histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a *** analysis,germ cell identification,and assessment of seminiferous tubules and maturation were *** assay was performed by radioimmunoassay using blood samples collected at regular ***:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular *** migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome *** and mature sperm cells were present when spermatogenesis phases were *** analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal ***,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal ***:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal *** also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.

关键词： Spermatogenesis Camel Testosterone profile Morphological staging

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A protective role of resveratrol against the effects of immobilization stress in corpora lutea of mice in early pregnancy

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Journal of Integrative Agriculture 2020年第7期19卷 1857-1866页

作者： Saif ULLAH Sheeraz MUSTAFA wael ennab Muhammad JAN Muhammad SHAFIQ Ngekure MXKAVITA LU Zeng-peng MAO Da-gan SHI Fang-xiong College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095P.R.China

In the present study,we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea(CL)of mice in early pregnancy.A total of 45 early-pregnant mice were divided into no immobilization stress(NIS)group,immobilization stress(IS)group,and immobilization and resveratrol treatment(IS+RES)group(n=15).Mice were immobilized in plastic tubes(50 mL)for 3 h per day during day 1 to 7 of *** the IS+RES group,5 mg kg-'d-1 of resveratrol was administered just prior to application of *** analyzed apoptotic activity in CL by Western botting analysis(WB),transmission electron microscopy(TEM),and immunohistochemistry(IHC).Serum progesterone levels were examined with radioimmunoassay(RIA).IHC results showed that the intensity of positive staining for Bax was increased,and for BcI-2 was decreased in CL after IS,while resveratrol treatment reversed the positive staining for Bax and *** revealed that immobilization stress up-regulated the expression of Bax and caspase-9,and down-regulated Bcl-2 expression,while resveratrol treatment attenuated the effects of immobilization stress on the expression of Bax,Bcl-2 and *** to our TEM results,apoptosis as defined by chromatin condensation was found in CL after immobilization stress,while resveratrol inhibited the *** also demonstrated that immobilization stress decreased progesterone concentrations and ovarian expression of StAR,while resveratrol restored the concentrations of progesterone and expression of StAR back to *** results indicated that immobilization stress induced luteal regression while resveratrol inhibited luteal regression,suggesting that resveratrol plays a protective role on corpora lutea of mice during early pregnancy.

关键词： immobilization stress apoptosis corpora lutea resveratrol pregnant mice

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白藜芦醇对束缚应激小鼠胃和脾脏的保护作用

白藜芦醇对束缚应激小鼠胃和脾脏的保护作用

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作者： wael Ziad Hasan ennab 南京农业大学

学位级别：硕士

应激是现代社会不可避免的现象,应激的产生会导致许多生理和心理上的改变。内稳态紊乱被称为应激,内稳态会受到一种被称为应激源的刺激。一般来说,有三种类型的应激源:生理性、心理性和代谢性。动物研究中的应激源通常是混合型的,例如... 详细信息

应激是现代社会不可避免的现象,应激的产生会导致许多生理和心理上的改变。内稳态紊乱被称为应激,内稳态会受到一种被称为应激源的刺激。一般来说,有三种类型的应激源:生理性、心理性和代谢性。动物研究中的应激源通常是混合型的,例如束缚应激(Restraint Stress)是一种将生理性与心理性结合的应激源,限制动物的活动并将其与群体隔离。动物为了适应应激,其行为、生理和神经内分泌会发生很多改变。据报道,应激会影响动物的消化过程、生长性能、采食量和饮水量,并对动物的胃肠道、生殖器官和其他组织具有有害的影响。白藜芦醇是一种存在于葡萄、葡萄酒和蓝莓中的复合物。研究报道它具有多种药理作用,包括抗氧化、抗癌、细胞保护、抗血小板、抗衰老、抗炎和心脏保护作用。1白藜芦醇对束缚应激小鼠胃的保护作用为研究白藜芦醇能否减缓束缚应激对小鼠胃造成的不良影响,成年雄性小鼠被分为5组:对照组(C),束缚应激组(RS),束缚应激+赋形剂组(RS+V),束缚应激+2 mg白藜芦醇组(RS+2 mg RES),束缚应激+20 mg白藜芦醇组(RS+20 mg RES)。为建立束缚应激模型,小鼠每天被置于圆锥形离心管中4小时。连续15天给予试验组RS+V,RS+2 mg RES和RS+20 mg RES 口服对应剂量白藜芦醇。结果显示:与对照组相比,应激组RS,RS+V和RS+20 mg RES的采食量,饮水量和体重显著降低,而RS+20 mg RES没有明显变化。另外,应激组RS,RS+V和RS+20 mg RES的胃细胞形态出现急性损伤,胃壁细胞的数量下降,胃壁细胞的大小和直径减小,而RS+2 mg RES的胃组织形态正常。此外,凋亡诱导因子(Apoptosis-inducing factor)在 RS 和 RS+V 中表达更高,而在 RS+2 mg RES 和 RS+20 mg RES中的表达与对照组相似。我们的发现证明白藜芦醇能减轻束缚应激对小鼠胃的损伤。2白藜芦醇对束缚应激小鼠脾脏的保护作用本研究将50只成年雄性小鼠分为5组,每组10只:对照组(C),束缚应激组(RS),束缚应激+赋形剂组(RS+V),束缚应激+2 mg白藜芦醇组(RS+2 mg RES),束缚应激+20 mg白藜芦醇组(RS+20 mg RES)。为建立束缚应激模型并研究白藜芦醇能否减缓束缚应激对小鼠脾脏产生的不良影响,小鼠每天被置于圆锥形离心管中4小时。连续15天给予试验组RS+V,RS+2 mg RES和RS+20 mg RES 口服对应剂量白藜芦醇。结果显示:与对照组相比,应激组RS,RS+V和RS+20 mg RES的脾脏大小,重量和长度显著增加,而RS+2 mg RES没有明显变化。另外,应激组RS,RS+V和RS+20 mg RES的脾脏形态出现急性损伤,脾脏细胞的数量和分布下降,而RS+2 mg RES不存在这些现象。我们的研究证明束缚应激能导致脾肿大,并且白藜芦醇能改善束缚应激对脾脏造成的负面影响。总之,我们的研究显示白藜芦醇能改善束缚应激小鼠的健康,对束缚应激小鼠胃和脾脏损伤具有保护作用。

关键词：白藜芦醇束缚应激凋亡诱导因子胃壁细胞胃脾脏

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