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Heavy Metal Remediation in Agoè Nyivé Landfill Soils: Enhancing Stability through Organic Amendments

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心脏超声联合颈部血管超声检查在老年冠心病检出中的应用

Open Journal of Applied Sciences（应用科学） 2024年第4期 961页

作者： Bassaï Magnoudéwa Bodjona Diyakadola Dihéénane Bafai Gado Tchangbedji

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实用医学影像杂志 2024年第5期25卷 339-342页

作者：王萍瓦增成韩灿青海红十字医院超声科西宁810000

目的观察探讨老年冠心病患者临床诊断过程中心脏超声与颈部血管超声联合应用价值。方法选择青海红十字医院2022年1月至2023年11月收治100例疑似老年冠心病患者为研究对象,入院后均进行心脏超声、颈部血管超声检查,比较老年冠心病患者与... 详细信息

目的观察探讨老年冠心病患者临床诊断过程中心脏超声与颈部血管超声联合应用价值。方法选择青海红十字医院2022年1月至2023年11月收治100例疑似老年冠心病患者为研究对象,入院后均进行心脏超声、颈部血管超声检查,比较老年冠心病患者与老年非冠心病患者、单支与多支血管病变患者的斑块积分、心肌综合指数、颈动脉内膜中层厚度;并参照冠状动脉造影结果,计算心脏超声单一诊断效能、颈部血管超声单一诊断效能、心脏超声与颈部血管超声联合诊断效能。结果冠状动脉造影检查确诊89例老年冠心病患者,心脏超声与颈部血管超声联合诊断符合率88.0%高于心脏超声单一诊断符合率75.0%、颈部血管超声单一诊断符合率77.0%(P<0.05)。老年冠心病患者斑块积分、心肌综合指数、颈动脉内膜中层厚度高于老年非冠心病患者(P<0.05)。多支血管病变患者斑块积分、心肌综合指数、颈动脉内膜中层厚度高于单支血管病变患者(P<0.05)。以冠状动脉造影为金标准,心脏超声与颈部血管超声联合诊断准确性95.0%、灵敏度96.6%,均高于心脏超声单一诊断准确性(78.0%)、灵敏度(79.8%)与颈部血管超声单一诊断准确性(78.0%、80.9%)(P<0.05)。结论老年冠心病患者临床诊断过程中心脏超声与颈部血管超声联合应用价值更高,能够为临床准确诊断及病情评估提供重要参考。

关键词：冠心病老年人超声检查诊断效能

Laparoscopic Appendectomies at Brazzaville University Hospital

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Surgical Science 2024年第4期15卷 183-194页

作者： Murielle Etiennette Julie Note Madzele Pierlesky Elion Ossibi Bhodeho Medi Monwongui Didace Massamba Miabaou Giresse Bienvenu Tsouassa wa Ngono Prude Pertinie Avala Gilles Niengo Outsouta Noé Henschel Motoula Latou Carmich Nzaka Moukala Cédrick Du Bonheur Alima Koya Gilbert fabrice Otiobanda Digestive Surgery Department Brazzaville University Hospital Brazzaville Republic of the Congo faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of the Congo General Intensive Care Unit Brazzaville University Hospital Brazzaville Republic of the Congo

Introduction: Acute appendicitis represents an urgent surgical condition, requiring the removal of the vermiform appendix. Laparoscopy is the standard surgical approach. This study aimed to outline the epidemiological, clinical, therapeutic, and outcome characteristics of patients undergoing laparoscopic surgery for acute appendicitis. Patients and Methods: A descriptive, retrospective analysis was carried out in the Emergency and Digestive Surgery departments of the University Hospital Center of Brazzaville, spanning two years from January 1, 2021, to December 31, 2022. The study included all patients who underwent laparoscopic surgery for non-complicated acute appendicitis. Results: The study included 12 cases of non-complicated acute appendicitis treated with urgent laparoscopy. The mean age of the patients was 37 ± 8.9 years, with a sex ratio of 0.7. The predominant symptom leading to consultation was pain in the right iliac fossa, with an average time to consultation of 6.4 ± 5.7 hours. Patient histories included diabetes in one case, obesity in two cases, and one case of prior abdominoplasty. During surgery, a phlegmonous appendix was observed in nine patients. The average duration of hospital stay was 3.6 ± 1.3 days, with all patients experiencing uncomplicated recoveries. Conclusion: The practice of emergency laparoscopic appendectomy is still relatively infrequent in our setting and presents an ongoing challenge.

关键词： Acute Appendicitis Appendectomy Laparoscopy Emergency

柴达木盆地昆特依盐湖可培养嗜盐细菌多样性研究

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生物技术通报 2024年第7期40卷 285-298页

作者：马想蓉马欣陈胤勋龙启福王嵘邢江娃青海大学医学部基础医学研究中心西宁810016

【目的】探究不同富集时间、培养盐度、稀释梯度、培养基等对氯化物型昆特依高盐盐湖可培养嗜盐细菌多样性的影响,确定嗜盐细菌的最佳分离培养条件,挖掘更多的环境嗜盐细菌资源。【方法】从昆特依盐湖中采集水泥混合样本,选取7种培养基... 详细信息

【目的】探究不同富集时间、培养盐度、稀释梯度、培养基等对氯化物型昆特依高盐盐湖可培养嗜盐细菌多样性的影响,确定嗜盐细菌的最佳分离培养条件,挖掘更多的环境嗜盐细菌资源。【方法】从昆特依盐湖中采集水泥混合样本,选取7种培养基和10%、18% NaCl两个盐度,采用富集培养法、平板稀释涂布法和平板分区划线法分离嗜盐菌;通过16S rRNA基因测序与BLAST序列比对确定菌株系统分类学地位。同时采用免培养Illumina MiSeq高通量测序技术分析昆特依盐湖细菌群落结构多样性特征。【结果】免培养昆特依盐湖样本中共鉴定到细菌39门64纲101目219科703属,其中假单胞菌门(Pseudomonadota)、厚壁菌门(Bacillota)、拟杆菌门(Bacteroidota)和放线菌门(Actinomycetota)为主要的优势菌门。根据菌落的大小、形态、颜色、光泽度、透明度、质地、隆起状态和边缘特征等,共分离出436株嗜盐菌,隶属于3门3纲6目9科28属77种,其中16株可能为潜在新种。3门为厚壁菌门(Bacillota)、假单胞菌门(Pseudomonadota)和放线菌门(Actinomycetota),且均为免培养测序结果中的优势菌门。在属水平上,芽孢杆菌属(Bacillus)、盐单胞菌属(Halomonas)、喜盐芽孢杆菌属(Halobacillus)、枝芽孢菌属(Virgibacillus)和卤水杆菌属(Salicola)为优势菌属。肿块芽孢杆菌属(Tuberibacillus)、越南蔷薇菌属(Rossellomorea)和谷氨酸杆菌属(Glutamicibacter)是首次从盐湖中分离得到。18% NaCl分离得到的菌株数量明显少于10% NaCl,且多样性较低,芽孢乳杆菌科(Sporolactobacillaceae)是该条件下分出的特有菌科。7种培养基中,1/2 RCA培养基分离效果最好。最佳富集培养时间为0、7和60 d,最佳样品稀释梯度为10^(-2)和10^(-3)。【结论】从昆特依盐湖中共分离出嗜盐细菌3门3纲6目9科28属77种。应用多种培养基,设置不同盐度、富集培养时间和稀释梯度等可显著提升可培养嗜盐菌的多样性。

关键词：嗜盐细菌生物多样性氯化物型盐湖分离培养基富集培养梯度稀释高通量测序

强化厌氧表达dld基因以提高大肠杆菌工程菌发酵产L-乳酸的光学纯度

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中国酿造 2024年第8期43卷 115-121页

作者：余杰李正王金华王永泽高娃赵筱湖北工业大学生命科学与健康工程学院湖北武汉430068 湖北工业大学发酵工程教育部重点实验室湖北武汉430068 湖北工业大学工业发酵省部共建协同创新中心湖北武汉430068

为去除廉价发酵原料中的D-乳酸从而提高发酵最终产物L-乳酸的光学纯度,该研究通过构建带有不同厌氧诱导启动子(pflBp6、pnirB、pflBp6-pnirB)的D-乳酸脱氢酶基因(dld)的表达质粒,并将其分别转入大肠杆菌(Escherichia coli)HBUT-L,加强... 详细信息

为去除廉价发酵原料中的D-乳酸从而提高发酵最终产物L-乳酸的光学纯度,该研究通过构建带有不同厌氧诱导启动子(pflBp6、pnirB、pflBp6-pnirB)的D-乳酸脱氢酶基因(dld)的表达质粒,并将其分别转入大肠杆菌(Escherichia coli)HBUT-L,加强其在发酵产L-乳酸的同时消除外源D-乳酸的能力。通过装液量与转速的单因素试验筛选最优的质粒表达条件及菌株,并在无机盐培养基与玉米浆培养基中进行发酵验证。结果表明,在装液量为200 mL/250 mL、转速为150 r/min的条件下,含有启动子pflBp6-pnirB的表达质粒的工程菌株HBUT-L4发酵18 h时D-乳酸脱氢酶活力最高,为81.1 U/g。在此条件下进行无机盐培养基和玉米浆培养基发酵时,工程菌株HBUT-L4相对于出发菌株HBUT-L,D-乳酸消耗速率分别提高250%、217%,L-乳酸的光学纯度分别从96.32%、98.48%提升至99.95%、99.98%。在大肠杆菌工程菌发酵L-乳酸的过程中,带有厌氧诱导启动子pflBp6-pnirB的表达质粒可提高D-乳酸脱氢酶活力,强化菌株消除外源D-乳酸,进而提高L-乳酸的光学纯度的能力,具有重要的工业化应用价值。

关键词： L-乳酸光学纯度启动子 D-乳酸脱氢酶基因大肠杆菌

Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

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Journal of Pharmaceutical Analysis 2012年第3期2卷 174-179页

作者： Gao-wa.a.deng School of Chemistry and Chemical Engineering Inner Mongolia University

A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(GOPS) was coated on the glass slides firstly on the slides,then poly-L-lysine(PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated *** modified slides were characterized using X-ray photoelectron spectroscopy(XPS) and atomic force microscopy(AFM).The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned *** control test was conducted by the bare glass slides *** cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou *** number of cervical exfoliative cells on the unmodified slides,PLL coated slides and GOPS-PLL coated slides was 10307300,32837226 and 41197280(n=12),*** data among the three different modification methods showed significant differences(one-way analysis of variance,ANOVA test,Po0.05).The cell capturing effect of the GOPS-PLL slide was the best among the three different modified *** addition,the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.

关键词： Glass slide Cervical exfoliative cells ThinPrep Pap smear

mir-98-5p、ALKBH1在肝门部胆管癌组织中表达及与临床病理特征的关系

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中华普外科手术学杂志（电子版） 2024年第2期18卷 184-187页

作者：乌吉斯古楞哈斯高娃内蒙古医科大学附属医院肝胆外科呼和浩特010050 内蒙古医科大学附属医院老年医学中心一区呼和浩特010050

目的分析肝门部胆管癌(HC)组织中微小RNA-98-5p(mir-98-5p)、ALKB同源蛋白1(ALKBH1)表达水平,并探讨其与患者临床病理特征及预后的关系。方法选取2011年6月至2017年6月行根治性手术的96例HC患者癌组织和相对应的癌旁正常组织(≥5 cm)为... 详细信息

目的分析肝门部胆管癌(HC)组织中微小RNA-98-5p(mir-98-5p)、ALKB同源蛋白1(ALKBH1)表达水平,并探讨其与患者临床病理特征及预后的关系。方法选取2011年6月至2017年6月行根治性手术的96例HC患者癌组织和相对应的癌旁正常组织(≥5 cm)为研究对象。实时荧光定量PCR(qRT-PCR)法检测组织中mir-98-5p、ALKBH1 mRNA表达水平,免疫组织化学法检测组织中ALKBH1表达。用SPSS 25.0对数据进行统计学分析,计量资料以(x±s)表示,行独立样本t检验;计数资料行χ^(2)检验;使用Pearson相关分析HC组织中mir-98-5p与ALKBH1 mRNA表达水平的关系;使用Kaplan-Meier进行生存曲线分析mir-98-5p、ALKBH1与HC患者5年内总生存率的关系。P<0.05为差异有统计学意义。结果与癌旁正常组织相比,HC组织中ALKBH1 mRNA相对表达水平、ALKBH1阳性率较高(P<0.05),mir-98-5p相对表达水平较低(P<0.05);mir-98-5p、ALKBH1与HC患者淋巴结转移、TNM分期相关(P<0.05)。Pearson相关分析显示,HC组织中mir-98-5p与ALKBH1 mRNA表达水平呈负相关(P<0.05)。Kaplan-Meier生存分析显示,mir-98-5p低表达组5年累计生存率为18.8%,低于mir-98-5p高表达组33.3%(P<0.05);ALKBH1阳性组5年累计生存率为14.0%,低于ALKBH1阴性组43.6%(P<0.05)。结论mir-98-5p低表达、ALKBH1高表达与HC患者淋巴结转移、TNM分期及预后相关。

关键词：胆管肿瘤微小RNA-98-5p ALKB同源蛋白1 临床病理特征

Acceptability of Immunization against COVID-19 by the Populations of the Kasenga State Health Area in the Uvira Health Zone, DR Congo

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Journal of Immune Based Therapies, Vaccines and Antimicrobials 2024年第3期13卷 33-46页

作者： Derrick Bushobole Akiba Eric Amuri Madabali Robert Bushambale fataki Abel Asende Luhendama Jacques Mutono Matongo faustin Bukuru Mudage Christian Banyakwa Mitunda Saili Stay Mushobekwa Michel Byaombe wa Ngene Martin Longolongo Kiza Paulin Mulogoto Rushanika Emmanuel Nirambo Rujanjika Henry Manya Mboni Criss Koba Mjumbe Department of Public Health Higher Institute of Medical Techniques of Uvira (ISTM-Uvira) South Kivu DR Congo Department of Laboratory Techniques Higher Institute of Medical Techniques of Uvira (ISTM-Uvira) South Kivu DR Congo Nursing Science Sector Higher Institute of Medical Techniques of Uvira (ISTM-Uvira) South Kivu DR Congo Department of Public Health University of Lubumbashi Lubumbashi DR Congo Department of Pharmaceutical Techniques University of Lubumbashi DR Congo Department of Pharmaceutical Techniques Higher Institute of Medical Techniques of Uvira (ISTM-Uvira) South Kivu DR Congo

Introduction: COVID-19 was an emerging disease putting all public health systems in countries around the world in a state of emergency. To be able to prevent its spread and morbidity and mortality, several appropriate strategies were necessary, such as vaccination. The latter has been the subject of controversy. The objective of the present study is therefore to evaluate the factors associated with the acceptance of this vaccine within the population of the Kasenga State Health Area. A result which will shed light on future strategies to be put in place for possible new vaccines. Methodology: Is a prospective and analytical cross-sectional study conducted over a period of approximately 1 month from January 5 to February 5, 2024. A survey questionnaire in Kobotoolbox was useful for collecting data. STATA software was very important for us in analyzing the data collected. Results: Prevalence of vaccination against COVID-19 among the population of the Kasenga State Health Area is 37.5% (28.4 - 45.6). The study revealed that reluctance is observed among most of the population for different reasons, including, first and foremost, the deliberate aspect of not wanting to take the vaccine (46.6%) and rumors that this antigen is dangerous and harmful (32.9%). 72.5% of respondents believe that the COVID-19 vaccine is a fabrication, unhealthy and that the disease itself never existed. The study proved that there was a statistical relationship between age (p = 0.001) and adherence to vaccination. And the refusal of respondents to recommend the vaccine to loved ones was a factor associated with non-adherence to vaccination (OR = 7.901, 95% IC [3.028 - 20.615], p = 0.000). Conclusion: Vaccination against COVID-19 was not well accepted by the population of the study site. Raising public awareness and involving community leaders and political-administrative authorities, which has not been done well, would play an important role in the good perception of the disease, of the vaccine

关键词： COVID-19 Vaccination Kasenga State Health Area Associated factors Uvira Health Zone City of Uvira

Fluid flow and heat transfer characteristics of spiral coiled tube: Effects of Reynolds number and curvature ratio

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Journal of Central South University 2012年第2期19卷 471-476页

作者： YOO Geun-jong CHOI Hoon-ki DONG wa-ryong School of Mechatronics Changwon National University

Numerical analysis was performed to investigate flow and heat transfer characteristics in spiral coiled tube heat exchanger. Radius of curvature of the spiral coiled tube was gradually increased as total rotating angle reached 12n. As the varying radius of curvature became a dominant flow parameter, three-dimensional flow analysis was performed to this flow together with different Reynolds numbers while constant wall heat flux condition was set in thermal field. From the analysis, centrifugal force due to curvature effect is found to have significant role in behavior of pressure drop and heat transfer. The centrifugal force enhances pressure drop and heat transfer to have generally higher values in the spiral coiled tube than those in the straight tube. Even then, friction factor and Nusselt number are found to follow the proportionality with square root of the Dean number. Individual effect of flow parameters of Reynolds number and curvature ratio was investigated and effect of Reynolds number is found to be stronger than that of curvature effect.

关键词： spiral coiled tube heat exchanger Reynolds number curvature ratio secondary flow friction factor Nusselt number