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Pinellia Total Alkaloids Modulate the GABAergic System in Hippocampal Formation on Pilocarpine-Induced Epileptic Rats

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Chinese Journal of Integrative Medicine 2020年第2期26卷 138-145页

作者： DENG Chu-xin wu zhi-bing CHEN Yi YU Zheng-miao The First Clinical Medical School Guangzhou University of Chinese MedicineGuangzhou(510406)China Cerebropathy Center The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou(510406)China

Objective:To investigate the neuromodulatory effect of pinellia total alkaloids(PTA)on the gamma-aminobutyric acidergic(GABAergic)system in epileptic rats,and preliminarily evaluate the anti-epileptic effect of ***:Ninety-one male Sprague-Dawley rats were randomized to a control group(n=17)or an epileptic group(n=74)using computer-generated random *** epilepticus(SE)was induced with pilocarpine in the epileptic *** rats that survived SE were randomly divided into 4 groups,namely an epilepsy group(n=13),a topiramate(TPM,60 mg/kg)group(n=12),a high-dose PTA(800 mg/kg)group(n=12),and a low-dose PTA(400 mg/kg)group(n=10).Treatments were given intragastrically once daily for 14 *** control group and epilepsy group received normal *** recurrent seizures(SRSs)were monitored 8-h daily for 7 days after ***,the hippocampal formation tissues were *** level was measured using enzyme-linked immunosorbent *** and mRNA expression levels of glutamate decarboxylase 65(GAD65),GABA transporter-1(GAT-1),GABA transaminase(GABA-T),and GABAA receptor(GABAAR)α4,α5,γ2 andδsubunits were measured using Western-blotting analysis and quantitative polymerase chain ***:PTA lowered the incidence and frequency of SRS(both doses *** TPM group,P>0.05).Compared with the epilepsy group,PTA increased the levels of GABA(both doses P<0.01)and GAD65(mRNA,800 mg/kg,P<0.01),and suppressed the levels of GAT-1(mRNA,800 mg/kg,P<0.01;400 mg/kg,P<0.05),GABA-T(mRNA,both doses P<0.01),and GABAARδsubunit(protein,800 mg/kg,P<0.05)andγ2 subunit(protein,both doses P<0.01).PTA upregulated the low-expressed mRNA levels of GABAARα5 subunit(400 mg/kg,P<0.01),δsubunit(800 mg/kg,P<0.05),andγ2 subunit(400 mg/kg,P<0.05).Conclusions:PTA regulated the GABAergic system through modulating GABA levels and the expression levels of GAD65,GAT-1,GABA-T,and GABAARα4,α5,γ2 andδ*** may exert antiepileptic effects on the pilocarpine-

关键词： Pineilia total alkaloids Pineilia ternata gamma-aminobutyric acidergic agents seizure epilepsy

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Acute Cerebellar Infarction Complicated with Multiple Intracerebral Hemorrhage Treated by An Integrated Chinese and Western Medicine Approach:A Case Report

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Chinese Journal of Integrative Medicine 2017年第3期23卷 221-225页

作者： CHEN Peng SHEN Qin-xuan SHEN Lin-yan wu zhi-bing PI Li-hong GE Wen-hua QI Wei Department of Neurology the First Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou(510006) China Department of Rehabilitation Medicine the First Affiliated Hospital of Guangzhou Medical University Guangzhou (510006) China

Cerebellar infarction accounts for almost 3%of all ischemic strokes,with 27,400 new cases each year in the USA alone.（1）Since the computerized tomography（CT）scan is often negative in the early hours after onset,it... 详细信息

关键词： computerized cerebellar alone consciousness decoction stasis vomiting intravenous infusion coagulation

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Spatio-Temporal Expression Patterns of Aurora Kinases A, B in Mouse Zygotes during the First Mitosis

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Journal of Reproduction and Contraception 2011年第1期22卷 11-21页

作者： Jie ZHANG Lin XU Guo-li WANG Divya RANA Tong LIU Di-di wu bing-zhi YU Department of Biochemistry and Molecular Biology China Medical University Shenyang 110001 China Department of Gynecology and Obstetrics Shengjing Hospital of China Medical University Shenyang110003 China

Objective To investigate the expression and localization of Aurora kinase A （A URKA ） and Aurora kinase B （A URKB） in mouse zygotes during the process of the first mitosis. Methods Quantitative real-time RT-PCR and Western blotting were performed to analyze the expression of AURKA and AURKB. The subcellular location of AURKA and A URKB was studied by confocal microscopy. Results A URKA and A URKB were increasingly expressed from phase G1 and peaked at phase M. After the entrance into mitosis A URKB became the predominant form both in mRNA and protein levels. The proteins of A URKA and A URKB both distributed in the cytoplasm and were associated with nucleus during the first mitosis of mouse zygotes, with some details in different. Conclusion The expression and localization of Aurora kinases A and B was in a cell- cycle regulated manner during the process of the cleavage of mouse zygotes. This discovery will aid in future investigations on their specific roles and molecular mecha- nisms in the regulation of mammalian early embryonic development.

关键词： Aurora kinase mouse zygote localization cell cycle regulation

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Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

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World Journal of Gastroenterology 2001年第5期7卷 597-601页

作者： Zhao-You Tang Fan-Xian Sun Jian Tian Sheng-Long Ye Yin-Kun Liu Kang-Da Liu Qiong Xue Jie Chen Jing-Lin Xia Lun-Xiu Qin Hui-Chuan Sun Lu Wang Jian Zhou Yan Li Zeng-Chen Ma Xin-Da Zhou zhi-Quan wu zhi-Ying Lin bing-Hui Yang Liver Cancer Institute of Fudan University and Zhongshan Hospital,Shanghai 200032,China Liver Cancer Institute of Fudan University (previous Liver Cancer Institute of Shanghai Medical University)136 Yixueyuan Road Zhongshan Hospital Shanghai 200032China. zytang@***

Metastatic human HCC model is needed for the studies on mechanism and intervention of metastatic recurrence. By using orthotopic implantation of histologically intact tissues of 30 surgical specimens, a patient-like metastatic model of human HCC in nude mice (LCI-D20) and a low metastatic model of human HCC in nude mice (LCI-D35) have been established. All mice with transplanted LCI-D20 tumors exhibited extremely high metastatic ability including spontaneous metastasis to liver, lungs, lymph nodes and peritoneal seeding. Remarkable difference was also found in expression of some of the invasiveness related genes and growth factors between the LCI-D20 and LCI-D35 tumors. PAI-1 increased gradually following tumor progression in LCI-D20 model, and correlated with tumor size and AFP level. Phasic expression of tissue intercellular adhesion molecule-1 in this model was also observed. Using corneal micropocket model, it was demonstrated that the vascular response induced by LCI-D20 tumor was stronger than that induced by LCI-D35 tumor. Similar report on metastatic human HCC model in nude mice and human HCC cell line with metastatic potential was rarely found in the literature. This LCI-D20 model has been widely used for the studies on intervention of metastasis, including anti-angiogenesis,antisense approach, metalloproteinase inhibitor, differentiation inducer, etc. It is concluded that the establishment of metastatic human HCC model in nude mice and human HCC cell line with metastatic potential will provide important models for the in vitro and in vitro study of HCC invasiveness, angiogenesis as well as intervention of HCC recurrence.

关键词： Animals Carcinoma, Hepatocellular Disease Models, Animal Humans Liver Neoplasms, Experimental Mice Mice, Nude Research Support, Non-U.S. Gov't Tumor Cells, Cultured

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客运专线动车组运用优化研究

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交通运输工程与信息学报 2011年第2期9卷 78-82,121页

作者：吴冰芝西南交通大学交通运输学院成都610031

结合国内对客运专线动车组运用优化的已有研究,分析了动车组运用的核心问题。结合动车组运用计划的编制原则,建立了列车运行图已知、列车成对运行、动车组不固定区段使用模式、动车组检修地点和时间已知、动车组均使用同一型号和编组条... 详细信息

结合国内对客运专线动车组运用优化的已有研究,分析了动车组运用的核心问题。结合动车组运用计划的编制原则,建立了列车运行图已知、列车成对运行、动车组不固定区段使用模式、动车组检修地点和时间已知、动车组均使用同一型号和编组条件下,动车组运用计划编制的数学模型。将这些条件下的动车组接续运行视为一个具有多目标和多约束条件的TSP(旅行推销商问题)问题,运用元胞蚁群算法对问题进行求解。以武广客运专线为例,验证了模型和算法的实用性,将元胞蚁群算法计算机编制的结果与人工编制方法的结果进行了比较,得出完成给定运行图任务所需的动车组数量。

关键词：客运专线动车组运用优化检修数学模型元胞蚁群算法

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基于iTRAQ标记联合IPA生物信息分析探究大黄素镇痛作用的机制

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中国药理学通报 2020年第12期36卷 1724-1730页

作者：陈鹏王琛张杰吴智兵吴远华贵州中医药大学基础医学院贵州贵阳550025 广州中医药大学第一临床医学院广东广州510405 贵州中医药大学第一附属医院贵州贵阳550001

目的探究大黄素在神经病理性疼痛慢性压迫性损伤(chronic constriction injury,CCI)模型中的镇痛机制。方法将SD大鼠随机分为假手术组、模型组及大黄素组。术后d 1开始,大黄素组给予50 mg·kg^-1大黄素溶液灌胃,每日1次,连续15 d。15 d... 详细信息

目的探究大黄素在神经病理性疼痛慢性压迫性损伤(chronic constriction injury,CCI)模型中的镇痛机制。方法将SD大鼠随机分为假手术组、模型组及大黄素组。术后d 1开始,大黄素组给予50 mg·kg^-1大黄素溶液灌胃,每日1次,连续15 d。15 d后取术侧L4~L6脊髓组织,运用蛋白质组学技术筛选差异表达蛋白,并进行IPA分析。结果模型组与假手术组筛选出差异蛋白102个。大黄素组与模型组筛选出差异蛋白109个,差异蛋白主要参与的经典通路为LXR/RXR激活、脊髓背角神经元神经病理性疼痛信号、突触长时程增强信号等。上游调控因子分析发现TCF7L2及APP是大黄素干预下差异蛋白可能的上游调控分子。最后蛋白互作网络分析结果发现,大黄素通过干预CaMKⅡ、PKA及PP2A等差异蛋白表达,调节“细胞间信号与相互作用、药物代谢、神经系统发育和功能”蛋白网络。结论大黄素通过调控神经病理性疼痛多条信号通路发挥镇痛作用。

关键词：神经病理性疼痛大黄素 iTRAQ IPA分析差异表达蛋白信号通路

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Overview of in-situ oxygen production technologies for lunar resources

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 233-255页

作者： Youpeng Xu Sheng Pang Liangwei Cong Guoyu Qian Dong Wang Laishi Li Yusheng wu zhi Wang College of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center for Green Recycling of Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

The rich resources and unique environment of the Moon make it an ideal location for human expansion and the utilization of extraterrestrial ***,crucial for supporting human life on the Moon,can be extracted from lunar regolith,which is highly rich in oxygen and contains polymetallic *** oxygen and metal extraction can be achieved using existing metallurgical ***,the ample reserves of water ice on the Moon offer another means for oxygen *** paper offers a detailed overview of the leading technologies for achieving oxygen production on the Moon,drawing from an analysis of lunar resources and environmental *** delves into the principles,processes,advantages,and drawbacks of water-ice electrolysis,two-step oxygen production from lunar regolith,and one-step oxygen production from lunar *** two-step methods involve hydrogen reduction,carbothermal reduction,and hydrometallurgy,while the one-step methods encompass fluorination/chlorination,high-temperature decomposition,molten salt electrolysis,and molten regolith electrolysis(MOE).Following a thorough comparison of raw materials,equipment,technology,and economic viability,MOE is identified as the most promising approach for future in-situ oxygen production on the *** the corrosion characteristics of molten lunar regolith at high temperatures,along with the Moon's low-gravity environment,the development of inexpensive and stable inert anodes and electrolysis devices that can easily collect oxygen is critical for promoting MOE technology on the *** review significantly contributes to our understanding of in-situ oxygen production technologies on the Moon and supports upcoming lunar exploration initiatives.

关键词： lunar resources in-situ oxygen production space metallurgy molten lunar regolith electrolysis

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美国继续工程教育对我国的启示

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扬州大学学报（高教研究版） 2005年第6期9卷 45-47页

作者：吴治兵扬州大学教育科学与技术学院江苏扬州225002

对科技人员开展继续工程教育是新时期我国走新型工业化道路的需要,是企业获得持续发展的力量之源,是科技人员适应知识经济时代挑战的必然选择。美国继续工程教育有着悠久的历史,其社会各界的重视程度很高,拥有多元化的办学主体,形成了... 详细信息

对科技人员开展继续工程教育是新时期我国走新型工业化道路的需要,是企业获得持续发展的力量之源,是科技人员适应知识经济时代挑战的必然选择。美国继续工程教育有着悠久的历史,其社会各界的重视程度很高,拥有多元化的办学主体,形成了相对成熟的继续工程教育体系,这些都对相对落后的我国继续工程教育有着极大的启示。

关键词：美国继续工程教育继续工程教育继续工程教育体系

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网络环境下如何提高书目情报服务质量

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河南图书馆学刊 2005年第2期25卷 32-34页

作者：吴冰芝台州学院图书馆浙江临海317000

书目情报服务是信息服务业的重要组成部分,因而提高书目情报服务质量有着重要的意义。文章分析了网络环境下书目情报服务的发展趋势,提出了改进和提高书目情报服务质量的途径。

关键词：书目情报服务网络环境信息服务

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对可见光有良好吸收的新型烷基芴共聚物的合成、表征与性质

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高等学校化学学报 2008年第2期29卷 332-335页

作者：吴江勇霍二福吴志兵李瑛谢明贵蒋青四川大学化学学院成都610064

以芴为起始原料，合成了两种主链含有芴酮及二腈基亚甲基芴结构单元的聚烷基芴光伏材料，通过红外光谱和核磁共振谱对其进行了表征．目标共聚物P1和P2的热分解温度分别为461．2和448．1℃，玻璃化转变温度（t）分别为122．4和146．6℃... 详细信息

以芴为起始原料，合成了两种主链含有芴酮及二腈基亚甲基芴结构单元的聚烷基芴光伏材料，通过红外光谱和核磁共振谱对其进行了表征．目标共聚物P1和P2的热分解温度分别为461．2和448．1℃，玻璃化转变温度（t）分别为122．4和146．6℃，具有良好的热稳定性．在300～700nm范围内，该种材料对太阳光的吸收基本覆盖了整个可见光区域，电化学分析得出其具有较好的氧化还原可逆性，并且具有1．94和1．86eV的相对较窄的能带宽度．

关键词：聚芴芴酮二腈基亚甲基芴 Suzuki偶联反应

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