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Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus

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mLife 2024年第1期3卷 129-142页

作者： Pingping wu Wenjuan Mo Tian Tian Kunfeng Song Yilin Lyu Haiyan Ren Jungang Zhou yao yu Hong Lu State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghaiChina Shanghai Engineering Research Center of Industrial Microorganisms ShanghaiChina

Kluyveromyces marxianus is a food-safe yeast with great potential for producing heterologous *** the yield in *** remains a challenge and incorporating large-scale functional modules poses a technical obstacle in *** address these issues,linear and circular yeast artificial chromosomes of ***(KmYACs)were constructed and loaded with disulfide bond formation modules from Pichia pastoris or *** modules contained up to seven genes with a maximum size of 15 *** carried telomeres either from *** or *** were transferred successfully into *** and stably propagated without affecting the normal growth of the host,regardless of the type of telomeres and configurations of *** increased the overall expression levels of disulfide bond formation genes and significantly enhanced the yield of various heterologous *** high-density fermentation,the use of KmYACs resulted in a glucoamylase yield of 16.8 g/l,the highest reported level to date in *** and metabolomic analysis of cells containing KmYACs suggested increased flavin adenine dinucleotide biosynthesis,enhanced flux entering the tricarboxylic acid cycle,and a preferred demand for lysine and arginine as features of cells overexpressing heterologous ***,supplementing lysine or arginine further improved the ***,KmYAC provides a powerful platform for manipulating large modules with enormous potential for industrial applications and fundamental *** the disulfide bond formation module via YACs proves to be an efficient strategy for improving the yield of heterologous proteins,and this strategy may be applied to optimize other microbial cell factories.

关键词： disulfide bond formation expression of heterologous proteins Kluyveromyces marxianus telomere yeast artificial chromosome

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Pd-catalyzed intermolecular Si–O formation via Si–C activation

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Science China Chemistry 2024年第8期67卷 2661-2669页

作者： Shuang Liu yu-Shu Chen Yichen wu Peng Wang State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesShanghai 200032China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistryand Material Technology of Ministry of EducationHangzhou Normal UniversityHangzhou 311121China

The development of efficient Si–O bond formation reaction with 100% atom-economy, excellent functional group tolerance, and broad scope under mild conditions is highly desired due to the prevalence of silanol, silyl ether, and their derivatives in synthetic chemistry and materials science. Here, we have realized the Pd-catalyzed Si–O formation reaction via a Si–C activation approach with 100% atom-economy by employing silacyclobutanes(SCBs) and various hydroxy-containing substrates, including water,alcohols, phenols, and silanols. This protocol features a broad substrate scope, remarkable functional compatibility and mild conditions, providing a series of silanols, silyl ethers in high efficiency. Notably, this protocol could also be used for selective protection of hydroxy functionalities, and for the access of a class of novel polymers containing Si–O main chain. Preliminary mechanistic studies unveiled that this reaction underwent a Pd-catalyzed concerted ring-opening mechanism.

关键词： Si–O formation Si–C activation palladium silacyclobutane silanol silyl ether

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Alignment of Track Oscillations during Tropical Cyclone Rapid Intensification

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Advances in Atmospheric Sciences 2024年第4期41卷 655-670页

作者： Tong XIE Liguang wu Yecheng FENG Jinghua yu Key Laboratory of Meteorological Disaster of Ministry of Education Nanjing University of Information Science and TechnologyNanjing 210044China Dept.of Atmospheric and Oceanic Sciences and Institute of Atmospheric Sciences Fudan UniversityShanghai 200438China CMA-FDU Joint Laboratory of Marine Meteorology Shanghai 200438China

Recent studies on tropical cyclone(TC)intensity change indicate that the development of a vertically aligned TC circulation is a key feature of its rapid intensification(RI),however,understanding how vortex alignment occurs remains a challenging topic in TC intensity change *** on the simulation outputs of North Atlantic Hurricane Wilma(2005)and western North Pacific Typhoon Rammasun(2014),vortex track oscillations at different vertical levels and their associated role in vortex alignment are examined to improve our understanding of the vortex alignment during RI of TCs with initial hurricane *** is found that vortex tracks at different vertical levels oscillate consistently in speed and direction during the RI of the two simulated *** the consistent track oscillation reduces the oscillation tilt during RI,the reduction of vortex tilt results mainly from the mean track before *** is also found that the vortex tilt is primarily due to the mean vortex track before and after *** track oscillations are closely associated with wavenumber-1 vortex Rossby waves that are dominant wavenumber-1 circulations in the TC inner-core *** study suggests that the dynamics of the wavenumber-1 vortex Rossby waves play an important role in the regulation of the physical processes associated with the track oscillation and vertical alignment of TCs.

关键词： tropical cyclone rapid intensification vortex tilt Rossby wave

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Computational fluid dynamics modeling of rapid pyrolysis of solid waste magnesium nitrate hydrate under different injection methods

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Chinese Journal of Chemical Engineering 2024年第2期66卷 224-237页

作者： Wenchang wu Kefan yu Liang Zhao Hui Dong SEP Key Laboratory of Eco-industry School of MetallurgyNortheastern UniversityShenyang 110819China Liaoning Engineering Research Center of Energy Saving and Green Low Carbon Technology for Process Industry Northeastern UniversityShenyang 110819China

This study developed a numerical model to efficiently treat solid waste magnesium nitrate hydrate through multi-step chemical *** model simulates two-phase flow,heat,and mass transfer processes in a pyrolysis furnace to improve the decomposition rate of magnesium *** performance of multi-nozzle and single-nozzle injection methods was evaluated,and the effects of primary and secondary nozzle flow ratios,velocity ratios,and secondary nozzle inclination angles on the decomposition rate were *** indicate that multi-nozzle injection has a higher conversion efficiency and decomposition rate than single-nozzle injection,with a 10.3%higher conversion rate under the design *** decomposition rate is primarily dependent on the average residence time of particles,which can be increased by decreasing flow rate and velocity ratios and increasing the inclination angle of secondary *** optimal parameters are injection flow ratio of 40%,injection velocity ratio of 0.6,and secondary nozzle inclination of 30°,corresponding to a maximum decomposition rate of 99.33%.

关键词： Multi-nozzle Computational fluid dynamics Thermal decomposition reaction Pyrolysis furnace

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Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics

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Food Science and Human Wellness 2024年第1期13卷 472-481页

作者： Wenjun Xue Wenzhu Zhao Sijia wu Zhipeng yu School of Food Science and Engineering Hainan UniversityHaikou 570228China College of Food Science and Engineering Bohai UniversityJinzhou 121013China Lab of Nutrition and Functional Food Jilin UniversityChangchun 130062China

The angiotensin-converting enzyme(ACE)inhibitory peptide NCW derived from Mizuhopecten yessoensis has been demonstrated to have significant in vivo anti-hypertensive effects,however,its anti-hypertensive mechanism is still not fully *** study established a UPLC-Q-TRAP-MS/MS-based widely targeted kidney metabolomics approach to explore the changes of kidney metabolic profiles and to clarify the antihypertensive mechanism of peptide NCW in spontaneously hypertensive rats(SHRs).Multivariate statistical analysis indicated that the kidney metabolic profiles were clearly separated between the SHR-NCW and SHRUntreated groups.A total of 85 metabolites were differentially regulated,and 16 metabolites were identified as potential kidney biomarkers,e.g.,3-hydroxybutyrate,malonic acid,deoxycytidine,and L-aspartic *** peptide NCW might regulate kidney metabolic disorder of SHRs to alleviate hypertension by suppressing inflammation and improving nitric oxide production under the regulation of linoleic acid metabolism,folate related pathways,synthesis and degradation of ketone bodies,pyrimidine metabolism,β-alanine metabolism,and retinal metabolism.

关键词： ACE inhibitory peptide Kidney Mechanism Metabolomics Spontaneously hypertensive rats

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Quantifying the 2022 drought and spatiotemporal evolution of TWSA in the Dongting Lake Basin over the past two decades

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Geodesy and Geodynamics 2024年第5期15卷 516-527页

作者： Guodong Xu Sulan Liu Siyu Cheng yu Zhang Xiaohui wu yunlong wu Institute of Seismology China Earthquake AdministrationWuhan 430071China School of Geography and Information Engineering China University of GeosciencesWuhan 430074China

Extreme hydrological events such as droughts and floods have been increasingly influenced by abnormal atmospheric disturbances caused by human activity and global *** Dongting Lake Basin(DLB)has experienced challenging circumstances over the past 20 years due to complex climatic variations,leading to extreme flooding and *** study aims to investigate the spatiotemporal variation in terrestrial water storage anomalies(TWSA)over the DLB using data from the GRACE/GRACE-FO and GLDAS spanning the latest two decades.A significant decline trend in TWSA is unveiled from July 2019 to May 2023,with the rate of change determined as-1.94 cm/year and-1.99 cm/year based on the GRACE/GRACE-FO and GLDAS,*** GRACE-Drought Severity Index(DSI)is employed to identify and evaluate the severity and spatiotemporal evolution of the 2022 drought event in the *** results accurately capture the drought event,which began in July 2022 and continued until March 2023,with the most severe conditions occurring in October 2022,when the GRACE-DSI value stood at-2.06 and the TWSA decreased by 15.24 cm and 33.51 cm relative to the same month in 2021 and 2020,***,the daily water level variation at the Chenglingji hydrological gauging station in 2022 broke previous records,reaching a minimum of only 19 *** the 2022 drought event with the drought events in 2006 and 2011,the impact of drought on vegetation growth conditions was relatively small,but there was still significant vegetation degradation across the DLB.

关键词： Dongting Lake drought Terrestrial water storage GRACE-DSI NDVI GRACE

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Integration of urchin-like MnCo_(2)O_(4)@C core–shell nanowire arrays within porous copper current collector for superior performance Li-ion battery anodes

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Rare Metals 2024年第2期43卷 599-611页

作者： Xue-Ru Zhang Qiong wu Yong Zhang Xi-Fei Li Ting Xie yu-Cheng wu Instrumental Analysis Center Hefei University of TechnologyHefei230009China Key Laboratory of Advanced Functional Materials and Devices of Anhui Province Hefei230009China School of Materials Science and Engineering Hefei University of TechnologyHefei230009China Institute of Advanced Electrochemical Energy Xi’an University of TechnologyXi’an710048China

Spinel MnCo_(2)O_(4) is a promising energy storage candidate as anode materials in lithium-ion batteries owing to synergistic effects of two intrinsic solid-state redox ***,low conductivity,poor rate capacity and rapid capacity fading have seriously impaired its practical *** overcome the inferiorities,urchin-like MnCo_(2)O_(4)@C core–shell nanowire arrays have been fabricated directly within a porous copper current collector via a facile hydrothermal method followed by a chemical vapor deposition carbonization *** a typical nanowire,the core is composed of interconnected MnCo_(2)O_(4)nanoparticles and the shell shows as a thin amorphous carbon *** integrated MnCo_(2)O_(4)@C/Cu structure could act as working anodes without using additives or polymer *** MnCo_(2)O_(4)@C/Cu possesses slightly longer Li-ion insertion/desertion pathway than that of MnCo_(2)O_(4)/Cu,the carbon shell could effectively prevent the pulverization of MnCo_(2)O_(4) and lower down charge transfer resistance and actively participate in Li-ion *** rearrangement of carbon atoms during lithiation/delithiation cycling could inhibit the formation of passive solid electrolyte interphase *** a result,the MnCo_(2)O_(4)@C/Cu electrode presents superior rate capacity(600 mAh·g^(−1) at 1 A·g^(−1)) and better stability(797 mAh·g^(−1) after 200 cycles at 100 mA·g^(−1)).The excellent reversible Li ion storage capacity,cycling stability and rate capacity endow MnCo_(2)O_(4)@C/Cu great potential as stable and high output integrated anode materials in Li-ion batteries.

关键词： MnCo_(2)O_(4)@C Urchin-like nanowire arrays Core-shell Integrated anode materials Lithium-ion batteries

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Fast strength-ductility synergistically adjusting of cold spray additive manufactured Cu deposits via electric pulse processing

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Chinese Journal of Aeronautics 2024年第5期37卷 558-569页

作者： Dong wu Yaxin XU Wenya LI Xiawei YANG yu SU State Key Laboratory of Solidification Processing School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Shaanxi Key Laboratory of Friction Welding Technologies School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China

Electric Pulse Processing(EPP)treatment was innovatively introduced to optimize the strength and ductility of the CSAMed Cu *** results show that EPP is an efficient and fast post-treatment to improve the strength and ductility(within tens of seconds).The larger the pulse current and number of pulses,the better the mechanical ***,this research found that when the heat input determined by pulse current and number of pulses exceeds a certain threshold(pulse current intensity is 2000 A,number of pulses is 10),increasing the number of repeat time could also effectively improve the mechanical properties.A tensile strength of 210 MPa and a ductility of 14.0%could be obtained with reasonable EPP parameters(pulse current intensity is 2000 A,number of pulses is 10,and repeat number is 2),which is similar to those of conventional annealing(e.g.,tensile strength is 272 MPa,elongation is 28.3%).The microstructure evolution analysis shows that EPP can effectively improve the bonding quality between the deposited particles by recrystallization,promote grain growth and the formation of twins,which is the main reason for the improvement of mechanical properties.

关键词： Cold spray additive manufacturing Copper deposit Mechanical property Electric pulse processing Post treatment

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Vibration Control of the Rail Grinding Vehicle with Abrasive Belt Based on Structural Optimization and Lightweight Design

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Chinese Journal of Mechanical Engineering 2024年第3期37卷 311-337页

作者： Wengang Fan Shuai Zhang Zhiwei wu Yi Liu Jiangnan yu School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Key Laboratory of Vehicle Advanced Manufacturing Measuring and Control TechnologyMinistry of EducationBeijing 100044China China Railway Materials Operation and Maintenance Technology Co. LtdBeijing 100036China

As a new grinding and maintenance technology,rail belt grinding shows significant advantages in many applications The dynamic characteristics of the rail belt grinding vehicle largely determines its grinding performance and service *** order to explore the vibration control method of the rail grinding vehicle with abrasive belt,the vibration response changes in structural optimization and lightweight design are respectively analyzed through transient response and random vibration simulations in this ***,the transient response simulation analysis of the rail grinding vehicle with abrasive belt is carried out under operating conditions and non-operating ***,the vibration control of the grinding vehicle is implemented by setting vibration isolation elements,optimizing the structure,and increasing ***,in order to further explore the dynamic characteristics of the rail grinding vehicle,the random vibration simulation analysis of the grinding vehicle is carried out under the condition of the horizontal irregularity of the American AAR6 ***,by replacing the Q235 steel frame material with 7075 aluminum alloy and LA43M magnesium alloy,both vibration control and lightweight design can be achieved *** results of transient dynamic response analysis show that the acceleration of most positions in the two working conditions exceeds the standard value in GB/T 17426-1998 *** optimizing the structure of the grinding vehicle in three ways,the average vibration acceleration of the whole car is reduced by about 55.1%from 15.6 m/s^(2) to 7.0 m/s^(2).The results of random vibration analysis show that the grinding vehicle with Q235 steel frame does not meet the safety conditions of 3σ.By changing frame material,the maximum vibration stress of the vehicle can be reduced from 240.7 MPa to 160.0 MPa and the weight of the grinding vehicle is reduced by about 21.7%from 1500 kg to 1175 *** modal analysis results indic

关键词： Vibration control Dynamic characteristics Structural optimization Lightweight design Modal analysis

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Decentralized Primary Frequency Regulation for Hybrid Multi-terminal Direct Current Power Systems Considering Multi-source Enhancement

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 958-970页

作者： Jialiang wu Zhen Wang Ruixu Liu yu Shan Chenxuan Wang the College of Electrical Engineering Zhejiang UniversityHangzhou 310027China the Economic Research Institute of State Grid Zhejiang Electric Power Co. Ltd.Hangzhou 310016China

Hybrid multi-terminal direct current(MTDC)transmission technology has been a research focus,and primary frequency regulation(FR)improvement in the receiving-end system is one of the problems to be *** paper presents a decentralized primary FR scheme for hybrid MTDC power systems considering multi-source enhancement to help suppress frequency disturbance in the receiving-end *** the converters only need local frequency or DC voltage signal input to respond to system disturbance without communication or a control center,i.e.,a decentralized control *** proposed scheme can activate appropriate power sources to assist in FR in various system disturbance severities with fine-designed thresholds,ensuring sufficient utilization of each power *** better balance FR performance and FR resource participation,an evaluation index is proposed and the parameter optimization problem is further ***,the validity of the proposed scheme is verified by simulations in MATLAB/Simulink.

关键词： Decentralized control scheme primary frequency regulation(FR) rate of change of frequency(ROCOF) system frequency disturbance

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