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Selenocysteine methyltransferase SMT catalyzed the synthesis of Se-methylselenocysteine to regulate the accumulation of glucosinolates and sulforaphane in broccoli

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Horticultural Plant Journal 2024年第5期10卷 1151-1164页

作者： qi wu Junwei Wang Yuxiao Tian Chunyan Zhou Shuxiang Mao qiuyun wu Ke Huang College of Horticulture Hunan Agricultural UniversityChangshaHunan 410128China Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding Ministry of EducationChangshaHunan 410128China Key Laboratory for Vegetable Biology of Hunan Province ChangshaHunan 410128China

Selenocysteine methyltransferase(SMT)is a key enzyme involved in the Se metabolism pathway,and it is responsible for the catalysis of Se-methylselenocysteine(SeMSC)compound *** studies showed that selenium treatment activated SMT expression and promoted the accumulation of glucosinolates(GSLs)and sulforaphane,but the roles and functional mechanisms of SMT in mediating GSLs and sulforaphane synthesis remain *** this study,we identified the BoSMT gene in broccoli and uncovered its roles in mediating GSLs *** assays revealed that BoSMT is involved in SeMSC biosynthesis in *** importantly,the contents of GSLs and sulforaphane were significantly increased in the BoSMT-overexpressing broccoli lines but decreased in the knockdown lines,suggesting that BoSMT played a positive role in regulating GSLs and sulforaphane *** evidence indicated that BoSMT-mediated overaccumulation of GSLs and sulforaphane might be due to the increase in the endogenous SeMSC *** with the mock(water)treatment,selenite-induced significantly increases of the SeMSC content in the BoSMT-knockdown plants partially compensated the phenotype of GSLs and sulforaphane *** with the mock treatment,exogenous SeMSC treatment significantly increased the contents of GSL and sulforaphane and activated GSL synthesis-related gene expression,suggesting that SeMSC acted as a positive regulator for GSL and sulforaphane *** findings provided novel insights into selenium-mediated GSLs and sulforaphane *** genetic manipulation of BoSMT might be a useful strategy for improving the dietary nutritional values of broccoli.

关键词： Selenocysteine methyltransferase Transgenic broccoli Se-methylselenocysteine Glucosinolate sulforaphane Selenium

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BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli

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Horticultural Plant Journal 2024年第2期10卷 488-502页

作者： qiuyun wu qi wu Yuxiao Tian Chunyan Zhou Shuxiang Mao Junwei Wang Ke Huang College of Horticulture Hunan Agricultural UniversityChangshaHunan 410128China Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding Ministry of EducationChangshaHunan 410128China Key Laboratory for Vegetable Biology of Hunan Province ChangshaHunan 410128China Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops Ministry of Agriculture and Rural Affairs of ChinaChangshaHunan 410128China

Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase(MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its ***, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli *** addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid(Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves;its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMAMY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants.

关键词： Brassica oleracea var.italica Myrosinase BoMY Sulforaphane Selenite treatment

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An aqueous BiI_(3)-Zn battery with dual mechanisms of Zn^(2+)(de)intercalation and I^(-)/I_(2)redox

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Journal of Energy Chemistry 2024年第2期89卷 670-678,I0014页

作者： qi Deng Fangzhong Liu Xiongwei wu Changzhu Li Weibin Zhou Bei Long State Key Laboratory of Utilization of Woody Oil Resource Key Laboratory of National Forestry and Grassland Administration on Utilization Science for Southern Woody Oil ResourcesHunan Provincial Key Laboratory of Oils&Fats Molecular Structure and FunctionHunan Academy of ForestryChangsha 410004HunanChina School of Chemistry Xiangtan UniversityXiangtan 411105HunanChina School of Chemistry and Materials Science Hunan Agricultural UniversityChangsha 410128HunanChina

The development of aqueous battery with dual mechanisms is now arousing more and more *** dual mechanisms of Zn^(2+)(de)intercalation and I^(-)/I_(2)redox bring unexpected ***,differing from previous studies using Zn I_(2)additive,this work designs an aqueous Bi I_(3)-Zn battery with selfsupplied I^(-).Ex situ tests reveal the conversion of Bi I_(3)into Bi(discharge)and Bi OI(charge)at the 1st cycle and the dissolved I^(-)in *** active I^(-)species enhances the specific capacity and discharge medium voltage of electrode as well as improves the generation of Zn dendrite and ***,the porous hard carbon is introduced to enhance the electronic/ionic conductivity and adsorb iodine species,proven by experimental and theoretical ***,the well-designed Bi I_(3)-Zn battery delivers a high reversible capacity of 182 m A h g^(-1)at 0.2 A g^(-1),an excellent rate capability with 88 m A h g^(-1)at 10 A g^(-1),and an impressive cyclability with 63%capacity retention over 20 K cycles at 10 A g^(-1).An excellent electrochemical performance is obtained even at a high mass loading of 6 mg cm^(-2).Moreover,a flexible quasi-solid-state Bi I_(3)-Zn battery exhibits satisfactory battery *** work provides a new idea for designing high-performance aqueous battery with dual mechanisms.

关键词： Aqueous BiI_(3)-Zn battery Dual mechanisms I^(-)-induced uniform zinc deposition Ultralong cyclic life Flexible quasi-solid-state battery

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A novel method for determining the resistivity of compressed superconducting materials

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Matter and Radiation at Extremes 2024年第4期9卷 1-3页

作者： Liling Sun qi wu Shu Cai Yang Ding Ho-kwang Mao Center for High Pressure Science and Technology Advanced Research Beijing 100094China Institute of Physics Chinese Academy of SciencesBeijing 100190China

The measurement of resistivity in a compressed material within a diamond anvil cell presents significant *** high-pressure exper-imental setup makes it difficult to directly measure the size changes induced by pressure in the three crystallographic directions of the *** this study,we introduce a novel and effective method that addresses these technical *** method is anticipated to offer a valuable foundation for high-pressure investigations on quantum materials,particularly those with anisotropic layered structures.

关键词： resistivity method foundation

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Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units

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Neural Regeneration Research 2024年第4期19卷 887-894页

作者： Yifang wu Jun Sun qi Lin Dapeng Wang Jian Hai Department of Neurosurgery Tongji HospitalSchool of MedicineTongji University Department of Pharmacy Institute of Medical SciencesShanghai Jiao Tong University School of Medicine

Upregulation of vascular endothelial growth factor A/basic fibroblast growth factor(VEGFA/b FGF)expression in the penumbra of cerebral ischemia can increase vascular volume,reduce lesion volume,and enhance neural cell proliferation and differentiation,thereby exerting neuroprotective ***,the beneficial effects of endogenous VEGFA/b FGF are limited as their expression is only transiently *** this study,we generated multilayered nanofiber membranes loaded with VEGFA/b FGF using layer-by-layer self-assembly and electrospinning *** found that a membrane containing 10 layers had an ideal ultrastructure and could efficiently and stably release growth factors for more than 1 *** 10-layered nanofiber membrane promoted brain microvascular endothelial cell tube formation and proliferation,inhibited neuronal apoptosis,upregulated the expression of tight junction proteins,and improved the viability of various cellular components of neurovascular units under conditions of oxygen/glucose ***,this nanofiber membrane decreased the expression of Janus kinase-2/signal transducer and activator of transcription-3(JAK2/STAT3),Bax/Bcl-2,and cleaved ***,this nanofiber membrane exhibits a neuroprotective effect on oxygen/glucose-deprived neurovascular units by inhibiting the JAK2/STAT3 pathway.

关键词： brain ischemia brain microvascular endothelial cell nanofiber membrane neurovascular unit

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Structural Optimization of the Inlet Header of Supercritical Carbon Dioxide Printed Circuit Board Heat Exchanger

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Journal of Thermal Science 2024年第4期33卷 1458-1467页

作者： WANG Yanquan LU Yuanwei WANG Yuanyuan HAN Xinlong wu Yuting GAO qi MOE Key Laboratory of Enhanced Heat Transfer and Energy Conservation Beijing Key Laboratory of Heat Transfer and Energy ConversionBeijing University of TechnologyBeijing100124China

Supercritical carbon dioxide printed circuit board heat exchangers are expected to be applied in third-generation solar thermal power ***,the uniformity of supercritical carbon dioxide entering the heat exchanger has a significant impact on the overall performance of the heat *** order to improve the uniformity of flow distribution in the inlet header,this article studies and optimizes the inlet header of a printed circuit board heat exchanger through numerical *** results indicate that when supercritical carbon dioxide flows through the header cavity,eddy currents will be generated,which will increase the uneven distribution of flow rate,while reducing the generation of eddy currents will improve the uniform distribution of flow *** the dimensionless factor of the inlet header is 6,the hyperbolic configuration is the optimal *** also reduced the eddy current region by adding transition segments,and the results showed that the structure was the best when the dilation angle was 10°,which reduced the non-uniformity by 21%compared to the hyperbolic configuration,providing guidance for engineering practice.

关键词： third-generation solar thermal power generation inlet header PCHE numerical simulation

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Tiered clustering-based management architecture in mega-satellite networks

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Science China(Information Sciences) 2024年第5期67卷 215-227页

作者： qi HAO Di ZHOU min SHENG Yan SHI Jiandong LI State Key Laboratory of Integrated Service Networks Xidian University

Hybrid orbital networking and the proliferation of satellites raise explosive management complexity in mega-satellite networks(MSNs) and prompt the management architecture to the hierarchy. In this regard, we propose a tiered clustering-based management architecture(TCMA) to realize the high-efficient and low-cost management performance. Specifically, based on the constrained satellite coverage, we derive an expression of the mapping relationship between the required control overhead of the controller interaction,the average path length of the proposed TCMA, and the corresponding number of management tiers and cluster size. The expression can determine the optimal number of tiers of the TCMA by minimizing the required flow table size between controllers, while ensuring high transmission efficiency. We then exhibit the elasticity of the hierarchical TCMA by adding the peer connections at the second-highest management tier to reduce maximum redundant paths. Simulation results verify the effectiveness of the proposed TCMA and illustrate that a 3-tier TCMA is sufficient for thousands of satellites.

关键词： mega-satellite networks hierarchical architecture average hop tiered structure clustering

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The microbial community,nutrient supply and crop yields differ along a potassium fertilizer gradient under wheat-maize double-cropping systems

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Journal of Integrative Agriculture 2024年第10期23卷 3592-3609页

作者： Zeli Li Fuli Fang Liang wu Feng Gao mingyang Li Benhang Li Kaidi wu Xiaomin Hu Shuo Wang Zhanbo Wei qi Chen min Zhang Zhiguang Liu Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection College of Resources andEnvironmentLinyi UniversityLinyi 276000China National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources College of Resources and theEnvironmentShandong Agricultural UniversityTai’an 271018China Key Laboratory of Crop Specific Fertilizer Ministry of Agriculture and Rural Affairs/Xinyangfeng Agricultural Technology Co.Ltd.Jingmen 448001China Institute of Applied Ecology Chinese Academy of SciencesShenyang 110000China

Soil microorganisms play critical roles in ecosystem ***,the relative impact of the potassium(K)fertilizer gradient on the microbial community in wheat-maize double-cropping systems remains *** this long-term field experiment(2008-2019),we researched bacterial and fungal diversity,composition,and community assemblage in the soil along a K fertilizer gradient in the wheat season(K0,no K fertilizer;K1,45 kg ha^(-1) K_(2)O;K_(2),90 kg ha^(-1)K_(2)O;K3,135 kg ha^(-1)K_(2)O)and in the maize season(K0,no K fertilizer;K_(1),150 kg ha^(-1) K_(2)O;K_(2),300 kg ha^(-1)K_(2)O;K_(3),450 kg ha^(-1)K_(2)O)using bacterial 16S rRNA and fungal internally transcribed spacer(ITS)*** observed that environmental variables,such as mean annual soil temperature(MAT)and precipitation,available K,ammonium,nitrate,and organic matter,impacted the soil bacterial and fungal communities,and their impacts varied with fertilizer treatments and crop ***,the relative abundance of bacteria involved in soil nutrient transformation(phylum Actinobacteria and class Alphaproteobacteria)in the wheat season was significantly increased compared to the maize season,and the optimal K fertilizer dosage(K2 treatment)boosted the relative bacterial abundance of soil nutrient transformation(genus Lactobacillus)and soil denitrification(phylum Proteobacteria)bacteria in the wheat *** abundance of the soil bacterial community promoting root growth and nutrient absorption(genus Herbaspirillum)in the maize season was improved compared to the wheat season,and the K2 treatment enhanced the bacterial abundance of soil nutrient transformation(genus MND1)and soil nitrogen cycling(genus Nitrospira)genera in the maize *** results indicated that the bacterial and fungal communities in the double-cropping system exhibited variable sensitivities and assembly mechanisms along a K fertilizer gradient,and microhabitats explained the largest amount of the variation in crop yields,a

关键词： potassium fertilizer gradient microbial community wheat-maize double cropping climate change yield

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Synergistic precipitant powered self-assembly of papain for cross-linked enzyme crystals preparation

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Particuology 2024年第2期85卷 102-112页

作者： qi Hao Pan Guo Honghai Wang min Su Chunli Li Yuqi Hu Weiyi Su School of Chemical Engineering and Technology Hebei University of TechnologyTianjin300130China National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Tianjin300130China

In this study,we prepared cross-linked enzyme crystals(CLECs)of papain to further broaden the application of the enzyme with high activity in extreme ***,papain crystals were successfully obtained based on the micro-batch,batch,and expanded batch crystallization ***,ammonium sulfate and polyethylene glycol 6000(PEG6000)were synergistically used as the precipitants,while L-cysteine was applied to enhance the activity of ***,the interaction between L-cysteine and papain was modeled by molecular docking *** was found that L-cysteine could form a hydrogen bond with aspartic acid residue(Asp)at site 158,and the electrostatic attraction with lysine residue(Lys)at site 156 was also quite *** the enzyme crystals were cross-linked by glutaraldehyde at optimized *** papain CLECs were identified by various methods,and it was found that the thermal stability and enzymatic activity both increased compared to the raw *** importantly,it could be applied at more rigorous conditions,for example,pH of 4.

关键词： Papain Cross-linked enzyme crystals L-cysteine Molecular docking Crystallization

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Hybrid Dynamic Variables-Dependent Event-Triggered Fuzzy Model Predictive Control

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 723-733页

作者： Xiongbo Wan Chaoling Zhang Fan Wei Chuan-Ke Zhang min wu IEEE the School of Automation China University of Geosciencesthe Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systemsand the Engineering Research Center of Intelligent Technology for Geo-ExplorationMinistry of EducationWuhan 430074China

This article focuses on dynamic event-triggered mechanism(DETM)-based model predictive control(MPC) for T-S fuzzy systems.A hybrid dynamic variables-dependent DETM is carefully devised,which includes a multiplicative dynamic variable and an additive dynamic *** addressed DETM-based fuzzy MPC issue is described as a “min-max” optimization problem(OP).To facilitate the co-design of the MPC controller and the weighting matrix of the DETM,an auxiliary OP is proposed based on a new Lyapunov function and a new robust positive invariant(RPI) set that contain the membership functions and the hybrid dynamic variables.A dynamic event-triggered fuzzy MPC algorithm is developed accordingly,whose recursive feasibility is analysed by employing the RPI *** the designed controller,the involved fuzzy system is ensured to be asymptotically *** examples show that the new DETM and DETM-based MPC algorithm have the advantages of reducing resource consumption while yielding the anticipated performance.

关键词： Dynamic event-triggered mechanism(DETM) hybrid dynamic variables model predictive control(MPC) robust positive invariant(RPI)set T-S fuzzy systems

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