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Rearrangements of microtubule cytoskeleton in stomatal closure of Arabidopsis induced by nitric oxide

Chinese Science Bulletin 2008年第6期53卷 848-852页

作者： ZHANG YongMei wu zhongyi WANG XueChen YU Rong College of Life Sciences Capital Normal University Beijing 100037 China Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China

NO (nitric oxide), known as a key signal molecule in plant, plays important roles in regulation of stomatal movement. In this study, microtubule dynamics and its possible mechanism in the NO signal pathway were investigated. The results were as follows: (i) In vivo stomatal aperture assays revealed that both vinblastine (microtubule-disrupting drug) and SNP (exogenous NO donor) prevented stomatal opening in the light, and vinblastine even could enhance the inhibitory effect of SNP, whereas taxol (a microtubule-stabilizing agent) was able to reduce this effect; (ii) microtubules in the opening Arabi- dopsis guard cells expressing GFP:α-tubulin-6 (AtGFP:α-tubulin-6) were organized in parallel, straight and dense bundles, radiating from the ventral side to the dorsal side, and most of them were localized perpendicularly to the ventral wall; (iii) under the same environmental conditions, treated with SNP for 30 min, the radial arrays of microtubules in guard cells began to break down, twisted partially and be- came oblique or exhibited a random pattern; (iv) furthermore, the involvement of cytosolic Ca2+ in this event was tested. Stomatal aperture assays revealed that BAPTA-AM (a chelator of Ca2+) greatly sup- pressed the effect of NO on stomatal closure; however, it did not show the same function on stomatal closure induced by vinblastine. When BAPTA-AM was added to the SNP-pretreated solution, the SNP-induced disordered microtubulue cytoskeleton in guard cells underwent rearrangement in a time-dependent manner. After 30 min of treatment with BAPTA-AM, the cortical microtubules resumed the original radial distribution, almost the same as the control. All this indicates that NO may promote rearrangement of microtubule cytoskeleton via elevation of [Ca2+]cyt (free Ca2+ concentration in the cy- toplasm), finally leading to stomatal closure.

关键词：氧化一氮微管细胞骨架气孔运动气孔关闭钙离子

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Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis

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aBIOTECH 2024年第3期5卷 339-350页

作者： Zhenggang Dai Keyong Zhao Dengyu Zheng Siyu Guo Huawen Zou zhongyi wu Chun Zhang College of Agriculture Yangtze UniversityJingzhou 434025China Institute of Biotechnology Beijing Academy of Agriculture and Forestry SciencesBeijing Key Laboratory of Agricultural Gene Resources and BiotechnologyBeijing 100097China

Basic helix-loop-helix(bHLH)transcription factors are widely distributed in eukaryotes,and in plants,they regulate many biological processes,such as cell differentiation,development,metabolism,and stress *** studies have focused on the roles of bHLH transcription factors in regulating growth,development,and stress responses in maize(Zea mays),even though such information would greatly benefit maize breeding *** this study,we cloned the maize transcription factor gene ZmbHLH36(Gene ID:100193615,GRMZM2G008691).ZmbHLH36 possesses conserved domains characteristic of the bHLH ***-qPCR analysis revealed that ZmbHLH36 was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress *** Arabidopsis(Arabidopsis thaliana)plants heterologously expressing ZmbHLH36 had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L^(−1) NaCl treatment as well as 0.2 mol L^(−1) mannitol *** analysis of soil-grown plants under stress showed that transgenic Arabidopsis plants harboring ZmbHLH36 exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic *** contents were lower and peroxidase activity was higher in ZmbHLH36-expressing Arabidopsis plants than in the corresponding non-transgenic ***36 localized to the nucleus when expressed in maize *** study provides a systematic analysis of the effects of ZmbHLH36 on root growth,development,and stress responses in transgenic Arabidopsis,laying a foundation for further analysis of its roles and molecular mechanisms in maize.

关键词： Heterologous expression Maize Root system Stress responses ZmbHLH36

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BIG to CNCB:An Exploratory Journey from Genomics to Bioinformation

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Bulletin of the Chinese Academy of Sciences 2024年第3期38卷 164-169页

作者： YANG Yungui XUE Yongbiao wu zhongyi YANG Huanming China National Center for Bioinformation Beijing 100101China Beijing Institute of Genomics Chinese Academy of SciencesBeijing 100101China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Sun Yat-sen University Guangzhou 510275China Beijing Genomics Institute Shenzhen 518080China

The Beijing Institute of Genomics(BIG)of the Chinese Academy of Sciences,as the leading Institute in Genomics,has walked through 20 year’s journey since being founded in November *** participating in the Human Genome Project(HGP)in completing the“1%task”to independently accomplishing the super-hybrid rice genome and other several national and international genome projects,BIG has made tremendous contributions in genomics research and development in *** 2024,bearing great ambition and responsibility,BIG is transformed to the China National Center for Bioinformation(CNCB),aiming to become a global hub in bioinformatics big data services,innovation,and *** the completion of its new infrastructure in 2027,CNCB is looking into a brighter future.

关键词： Human Genome Research Center(HGRC) Beijing Genomics Institute(BGI) Beijing Institute of Genomics(BIG) China National Center for Bioinformation(CNCB) Genomics,Proteomics&Bioinformatics(GPB) Human Genome Project(HGP)

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Transcriptomic analysis of wheat reveals possible resistance mechanism mediated by Yr10 to stripe rust

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Stress Biology 2023年第1期3卷 477-494页

作者： zhongyi wu Gaohua Zhang Ran Zhao Qi Gao Jinchen Zhao Xiaoxu Zhu Fangyan Wang Zhensheng Kang Xiaojing Wang State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences Northwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Crop Stress Biology for Arid Areas and College of Natural Resources and Environment Northwest A&F UniversityYangling 712100ShaanxiChina

Stripe rust,caused by Puccinia striiformis ***(Pst),is a catastrophic disease that threatens global wheat ***10 is a race-specific all-stage disease resistance gene in ***,the resistance mechanism of Yr10 is poorly ***,to elucidate the potential molecular mechanism mediated by Yr10,transcriptomic sequencing was performed at 0,18,and 48 h post-inoculation(hpi)of compatible wheat Avocet S(AvS)and incompatible near-isogenic line(NIL)AvS+Yr10 inoculated with Pst race ***,227,208,and 4050 differentially expressed genes(DEGs)were identified at 0,18,and 48 hpi between incompatible and compatible *** response of Yr10 to stripe rust involved various processes and activities,as indicated by the results of Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway ***,the response included photosynthesis,defense response to fungus,metabolic processes related to salicylic acid(SA)and jasmonic acid(JA),and activities related to reactive oxygen species(ROS).Ten candidate genes were selected for qRT-PCR verification and the results showed that the transcriptomic data was *** the functional analysis of candidate genes by the virus-induced gene silencing(VIGS)system,it was found that the gene TaHPPD(4-hydroxyphenylpyruvate dioxygenase)negatively regulated the resistance of wheat to stripe rust by affecting SA signaling,pathogenesis-related(PR)gene expression,and ROS *** study provides insight into Yr10-mediated resistance in wheat.

关键词： Transcriptome Yr10 Virus-induced gene silencing system (VIGS)system 4-hydroxyphenylpyruvate dioxygenase HPPD

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A NEW RAPID ACOUISITION SEQUENCE FOR SPREAD SPECTRUM SYSTEMS

A NEW RAPID ACOUISITION SEQUENCE FOR SPREAD SPECTRUM SYSTEMS

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1992 International Conference on Communication Technology ICCT’92

作者： Hou Ruiting wu zhongyi Zhang Naitong Harbin Institute of Technology

＜正＞The re-coding sequence which can be used as rapid acquisition sequence in spread spectrum systems is proposed in this paper. The optimum structure and synchronization performance of the re-coding sequence are *** result shows that if we use the re-coding,sequence as *** sequence,rapid acquisition will be realized on the condition of lower signal-to-noise ratio.

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NETN:Noise encode transfer network for clinical low-dose CT image generation

NETN:Noise encode transfer network for clinical low-dose CT ...

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第十七届中国体视学与图像分析学术会议

作者：李铭 WANG Jiping DU Qiang wu zhongyi 郑健 Department of Medical Imaging Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of Sciences Institute of Electronic Information Engineering Changchun University of Science and Technology

Current deep learning-based methods applied to x-ray low-dose imaging are mostly supervised methods that rely on Mayo Clinic data for ***,the Mayo Clinic data was synthesized by adding Poisson noise to the simulated projection map,which may not accurately model the underlying physical mechanism of low-dose computed tomography(LDCT) imaging,so that supervised methods are usually not well generalized to clinical *** solve the above problem,we propose the first deep learning method for generating clinical low-dose CT image dataset for supervised ***,we introduce a method to encode noise synthesis operators for generating an attenuation adaptive noise kernel ***,to generate clinical low-dose CT dataset with realistic noise distribution,we propose a transfer learning approach via transferring noise synthesis operators from simulated noise dataset to real noise *** addition,we innovatively introduce a noise control factor into the network testing phase,which can regulate the noise level of the generated low-dose CT *** experiments show that,compared with Mayo Clinic data,three supervised models trained based on low-dose data generated by our proposed method can be better generalized to clinical low-dose denoising tasks.

关键词： LDCT deep learning adaptive noise kernel space transfer learning

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运动经验的知觉现象研究——刘一民教授学术对话录

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体育与科学 2019年第4期40卷 18-24页

作者：居方圆吴忠谊台湾师范大学运动与休闲学院体育学系台北教育大学体育学系

时代在转变,体育学研究的范式也出现转移。学者倡导回归运动本身,梅洛-庞蒂在《知觉现象学》中所主张的身体—主体(body-subject),对于运动经验的研究具有意义。本研究目的为探讨运动经验采用现象学研究的意义以及如何让运动者进入情境... 详细信息

时代在转变,体育学研究的范式也出现转移。学者倡导回归运动本身,梅洛-庞蒂在《知觉现象学》中所主张的身体—主体(body-subject),对于运动经验的研究具有意义。本研究目的为探讨运动经验采用现象学研究的意义以及如何让运动者进入情境中心,与台湾运动哲学领域权威专家刘一民教授进行一次学术访谈。刘一民教授从台湾地区运动哲学研究出发,介绍现象学研究方法,强调切身感与场域性的重要性,与程志理教授的"体认"对话,并阐释了"体认"教学对幼儿体育课程设计的启示。

关键词：运动经验知觉现象学体认刘一民幼儿体育

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基于图斑空间关系的遥感专家分类方法研究

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武汉大学学报（信息科学版） 2006年第4期31卷 321-324页

作者：蔡晓斌陈晓玲王涛吴忠宜武汉大学测绘遥感信息工程国家重点实验室武汉市珞喻路129号430072

以光谱均质单元———图斑作为空间对象粒度的基本单元,采用图斑邻域搜索算法,获取不同类别图斑间的空间关系。在ERDAS专家分类系统中,根据图斑相邻关系以及DEM信息对初始分类结果进行了修正,提高了湿地、草地和农业用地的分类精度。

关键词：图斑空间关系专家知识

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关于中国公路灾害遥感监测数据源及其保障机制的思考

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公路交通科技 2011年第11期28卷 43-47,60页

作者：张勇刘向龙吴忠宜交通运输部科学研究院北京100013

探讨如何有效地建立遥感和基础路网数据的快速获取机制,为重大公路灾害遥感监测与评估提供数据保障。当前突出的问题是重大公路灾害后交通部门开展应急抢通保通缺乏快速有效的信息支持。通过归纳概括当前中国可用的遥感数据资源管理分... 详细信息

探讨如何有效地建立遥感和基础路网数据的快速获取机制,为重大公路灾害遥感监测与评估提供数据保障。当前突出的问题是重大公路灾害后交通部门开展应急抢通保通缺乏快速有效的信息支持。通过归纳概括当前中国可用的遥感数据资源管理分布情况,结合对遥感数据服务提供机制和公路地图数据管理的现状及存在问题的分析,提出了公路灾害遥感监测和评估的各类数据获取和使用保障机制建立模式的建议。认为当前交通部门只有尽快建立部门内部的数据使用机制和遥感数据获取手段,或尽快建立跨部门的遥感数据获取保障机制,才能保证重大公路灾害发生后能够获得及时有效的遥感监测服务。

关键词：道路工程数据保障机制现状分析公路灾害监测遥感

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快速压缩装置角涡运动特性

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哈尔滨工程大学学报 2011年第10期32卷 1341-1345页

作者：刘正先吴仲义郑雅芳罗纪生天津大学机械学院力学系天津300072

针对内燃机快速压缩装置(RCM)内部的典型涡旋流动,采用CFD方法对不同边界条件和变比例几何模型下的内燃机汽缸模型RCM内涡旋的形成与分布特征进行了数值模拟分析.结果表明:RCM的汽缸与活塞形成的角区附近存在一对反向旋转的涡旋,通常情... 详细信息

针对内燃机快速压缩装置(RCM)内部的典型涡旋流动,采用CFD方法对不同边界条件和变比例几何模型下的内燃机汽缸模型RCM内涡旋的形成与分布特征进行了数值模拟分析.结果表明:RCM的汽缸与活塞形成的角区附近存在一对反向旋转的涡旋,通常情况下,角区涡将充满整个活塞壁.模型的几何尺寸比例对RCM内部的涡流动有显著影响:随着几何尺寸的变化,在靠近活塞壁面附近会出现一对稳定的反向涡,也会出现一个主涡和多个辅涡共存的复杂流道状态.

关键词：内燃机快速压缩装置计算流体动力学方法模型尺度涡流

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