检索结果-南通市图书馆

Journal of Integrative Agriculture 2020年第3期19卷 775-784页

作者： YANG Ya-jun XU hong-xing wu zhi-hong LU Zhong-xian State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural Sciences

Midgut extracts play crucial roles in food digestion and *** evaluated the effect of pH on the profiles of the midgut extracts in rice leaffolder,Cnaphalocrocis medinalis and the degradation of activated Bt-toxins by the midgut extracts under different pH *** protease activity increased slightly with the increase with the simulated pH in the midgut extracts and the maximal protease activity was observed at pH 10.5,while an upward trend was observed as the pH of reaction buffer *** of chymotrypsin-like enzymes increased with pH,both in the buffer and midgut extracts,while the activity of trypsin-like enzyme was *** of the activated Cry2 A by the midgut extracts enhanced as the pH ***2 A was fully degraded into smaller segments at pH *** Cry1 C protein at pH 9.0-10.5 was partially degraded by the midgut *** Cry1 Aa and Cry1 Ac were partially degraded into fragments by the midgut extracts at high *** results will facilitate our further understanding of the interactions between *** and the Cry toxin.

关键词： Cnaphalocrocis medinalis Bacillus thuringiensis pH digestion degradation protease

Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis (Lepidoptera:Pyralidae)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2021年第8期20卷 2195-2203页

Midgut juice plays an important role in food digestion and detoxification in *** order to understand the potential of midgut juice of Cnaphalocrocis medinalis(Guenée)to degrade Bt proteins,the enzymatic activity of midgut juice and its degradation of Bt proteins(Cry2A,Cry1C,Cry1Aa,and Cry1Ac)were evaluated in this study through protease inhibitor *** activities of total protease in midgut juices were significantly inhibited by phenylmethylsulfonyl fluoride(PMSF),tosyl-L-lysine chloromethyl ketone(TLCK),pepstatin A and *** enzymatic activity of chymotrypsin was significantly inhibited by PMSF,and enzymatic activity of trypsin was significantly inhibited by ethylenediaminetetraacetic acid(EDTA),PMSF,tosyl phenylalanine chloromethyl ketone(TPCK),TLCK and trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane(E-64).EDTA could significantly inhibit the degradation of Cry2A by ***,PMSF,TPCK,and TLCK could inhibit the degradation of Cry1C and ***,PMSF,TPCK,TLCK,and E-64 could inhibit the degradation of *** results indicated that some protease inhibitors hindered various enzymatic activities in the larval midgut of ***,which may reduce the insect’s ability to degrade Bt *** findings may aid the application of protease inhibitors in the management of this insect pest in the future.

关键词： Cnaphalocrocis medinalis midgut juice protease inhibitor enzyme activity degradation Bt protein

What factors determine the mass elevation effect of the Tibetan Plateau?

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2020年第11期17卷 2742-2749页

作者： HAN Fang WAN Li wu hong-zhi ZHANG Bai-ping GAO Lan SONG Ge School of Civil Engineering and Architecture Shandong University of TechnologyZibo 255000China Sub-Institute of High and New Technology Standardization China National Institute of StandardizationBeijing 100191China Institute for Development Strategy of Science and Technology Qilu University of TechnologyShandong Academy of SciencesJinan 250014China Institute of Geographic Sciences and Natural Resources Research State Key Laboratory of Resources and Environmental Information SystemBeijing 100101China

The mass elevation effect(MEE)of the Tibetan Plateau(TP)has attracted the attention of geographers because of its significant influence on the Asian climate,snow line,timberline,and other important climate-ecological boundaries of the plateau and on global ecological *** recent years,much progress has been made in quantifying the MEE of ***,factors that affect the size of MEE have not been examined in depth,and the key factors still remain *** on quantification of MEE for each mountain basal elevation plot,this study identifies the factors that contribute significantly to MEE of the *** factors are considered,including mountain basal elevation,distance from the core zone of MEE,thermal continentality,maximum elevation,height difference,area,and difference of underlying surface(with the yearly max"Normalized Difference Vegetation Index"(NDVI)serving as a quantitative indicator).We also used these seven factors as independent variables to develop a multiple linear regression model for MEE of the *** show that:(1)the determination coefficient(R2)of the model reaches as high as 0.877,and the contributions of mountain basal elevation,distance from the core zone of MEE,thermal continentality,maximum elevation,topographical height difference,area,and NDVI are 39.77%,23.02%,14.48%,5.78%,11.41%,2.92%,and 2.62%,respectively,with mountain basal elevation and the distance from the core of MEE as the most important factors;(2)thermal continentality and MEE are significantly correlated,and maximum elevation only has a coupling relationship with MEE,with height difference and NDVI contributing little to *** study deepens our understanding of MEE and its forming factors in the Tibetan Plateau.

关键词： Tibetan Plateau Mass elevation effect Mountain basal elevation Influencing factors

Recent Advances in Technique and Clinical Outcomes of Minimally Invasive Spine Surgery in Adult Scoliosis

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2017年第21期130卷 2608-2615页

作者： Gang Liu Sen Liu Yu-zhi Zuo Qi-Yi Li zhi-hong wu Nan wu Ke-Yi Yu Gui-Xing Qiu Department of Orthopaedic Surgery Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730 China Beijing Key Laboratory for Genetic Research of Skeletal Deformity Beijing 100730 China Department of Central Laboratory F eking Union Medical College Hospital Peking Union Medical Coilege and Chinese Academy of Medical Sciences Beijing 100730 China

Objective： Conventional open spinal surgery of adult scoliosis can be performed from anterior, posterior, or combined approach. Minimally invasive spine surgery （MISS） was developed for the purpose of reducing the undesirable effects and complications. This review aimed to make a brief summary of recent studies of the approach and clinical outcomes of MISS in adult scoliosis. Data Sources： We conducted a systematic search from PubMed, Medline, EMBASE, and other literature databases to collect reports of surgical methods and clinical outcomes of MISS in treatment of adult scoliosis. Those reports were published up to March 2017 with the following key terms：＆quot;minimally invasive,＆quot; ＆quot;spine,＆quot; ＆quot;surgery,＆quot; and ＆quot;scoliosis.＆quot;Study Selection： The inclusion criteria of the articles were as followings： diagnosed with adult degenerative scoliosis （DS） or adult idiopathic scoliosis; underwent MISS or open surgery; with follow-up data. The articles involving patients with congenital scoliosis or unknown type were excluded and those without any follow-up data were also excluded from the study. The initial search yielded 233 articles. After title and abstract extraction, 29 English articles were selected for full-text review. Of those, 20 studies with 831 patients diagnosed with adult DS or adult idiopathic scoliosis were reviewed. Seventeen were retrospective studies, and three were prospective studies. Results： The surgical technique reported in these articles was direct or extreme lateral interbody fusion, axial lumbar interbody fusion, and transforaminal lumbar interbody fusion. Among the clinical outcomes of these studies, the operated levels was 3–7, operative time was 2.3–8.5 h. Both the Cobb angle of coronal major curve and evaluation of Oswestry Disability Index and Visual Analog Scale decreased after surgery. There were 323 complications reported in the 831 （38.9%） patients, including 150 （18.1%） motor or sensory deficits, and 111 （13.4

关键词： Adult Scoliosis Complications Minimally lnvasive Spine Surgery Outcomes Surgical Methods

Effect of Resveratrol on Preventing Steroid-induced Osteonecrosis in a Rabbit Model

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2016年第7期129卷 824-830页

作者： Ji-Liang Zhai Xi-Sheng Weng zhi-hong wu Shi-Gong Guo Department of Orthopaedic Surgery Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing 100730 China Department of Orthopaedic Surg'ery Charingcross Hospital London W6 8RF UK

Background： Prevention of osteonecrosis （ON） has seldom been addressed. The purpose of this study was to evaluate the effect of resveratrol on preventing steroid-induced ON in rabbits. Methods： Seventy-two rabbits were divided into four groups：（l） NEC （ON） group： thirty rabbits were treated with lipopolysaccharide （LPS） once, then with methylprednisolone （MPS） daily for 3 days; （2） PRE （prevention） group： thirty rabbits were given one dose of LPS, then MPS daily for 3 days, and resveratrol on day 0 and daily for 2 weeks：（3） RES （resveratrol） group： six rabbits were given resveratrol for 2 weeks but without LPS/MPS; （4） CON （control） group： six rabbits were given alcohol for 2 weeks but without LPS/MPS. Levels of plasma tissue-type plasminogen activator （t-PAL plasminogen activator inhibitor 1 （PAl-1）, thrombomodulin （TM）, vascular endothelial growth factor （VEGF）, maximum enhancement （ME） by magnetic resonance imaging, and ON incidence were evaluated. Results： The PRE group had a lower ON incidence than the NEC group, but with no significant differences at 2 weeks and 12 weeks. Tile RES and CON groups did not develop ON. TM and VEGF were significantly higher in the NEC group compared with the PRE group at weeks 1, 2, and 4 （TM： 1 week, P= 0.029; 2 weeks, P= 0.005; and 4 weeks, P = 0.047; VEGF： 1 week, P= 0.039; 2 weeks, P= 0.021; 4 weeks. P = 0.014） but the difference disappeared at 12 weeks. The levels of t-PA and PAl-I were not significantly different between the NEC and PRE groups. The TM, t-PA, PAl-l, and VEGF concentrations in the RES and CON groups did not change over time. Compared to the baseline, ME in the NEC group decreased significantly （P = 0.025） at week 1, increased significantly （P = 0.021） at week 2, and was decreased at week 12. The variance was insignificant in the PRE group. Conclusions： Resveratrol may improve blood supply to bone in a rabbit model of ON of the femoral head via anti-inflammatory effects to protect the vascular endothelium and

关键词： Contrast-enhanced Magnetic Resonance Imaging Dynamic Femoral Head Necrosis Osteonecrosis Resveratrol

Adiponectin receptor 1 mediates the difference in adiponectininduced prostaglandin E2 production in rheumatoid arthritis and osteoarthritis synovial fibroblasts

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2011年第23期124卷 3919-3924页

作者： ZUO Wei wu zhi-hong wu Nan DUAN Yuan-hui wu Ju-tai WANG Hai QIU Gui-xing Department of Orthopedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

The synovial fluid concentrations of adiponectin are significantly higher in patients with rheumatoid arthritis （RA） than in patients with osteoarthritis （OA）. Accumulating evidence suggests that adiponectin may be an inducer of inflammation in arthritis, but the mechanism remains unclear. The objectives of this study were to compare the expression levels of adiponectin receptors in rheumatoid arthritis synovial fibroblasts （RASF） and osteoarthritis synovial fibroblasts （OASF）, evaluate the roles of adiponectin receptors in adiponectin-induced prostaglandin E2 （PGE2） production, and then investigate the effects of a nonsteroidal anti-inflammatory drug （NSAID） and a cyclooxygenase （COX）-2-selective inhibitor on adiponectin-induced PGE2 release. Methods The expressions of adiponectin receptor 1 （AdipoR1） and AdipoR2 mRNA and protein in synovial fibroblasts from seven patients with RA and eight patients with OA undergoing total knee replacement were evaluated by real-time polymerase chain reaction, immunofluorescence microscopy and Western blotting analysis. Adiponectin-induced PGE2 production was detected by enzyme-linked immunosorbent assay. RNA interference against the AdipoR1 and AdipoR2 genes was performed to investigate the effects of the adiponectin receptors on adiponectin-induced PGE2 production in both RASF and OASE Results AdipoR1 and AdipoR2 mRNA and protein were expressed by both RASF and OASF. Compared with OASF, RASF exhibited higher levels of AdipoR1, but there was no significant difference for AdipoR2. Adiponectin induced the production of PGE2 by the synovial fibroblasts in a concentration-dependent manner, and this was more obvious in RASF. RNA interference showed that the difference may be mediated by the diverse distribution of AdipoRl. The adiponectin-induced PGE2 production was efficiently relieved by the NSAID and COX-2-selective inhibitor. Conclusion The present findings suggest that AdipoR1 may mediate the difference in adiponectin-induced PGE

关键词： adiponectin rheumatoid arthritis osteoarthritis prostaglandin E2 fibroblast

Lumbar Scoliosis Induction in Juvenile Dogs by Three？dimensional Modulation of Spinal Growth Using Nickel？Titanium Coil Springs

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2017年第21期130卷 2579-2584页

作者： Heng-Yan Zhang Qi-Yi Li zhi-hong wu Yu Zhao Gui-Xing Qiu Department of Orthopaedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

Background： Current treatments for scoliosis have some defects and complications. To study spinal deformities and test novel scoliosis treatments, many animal models of scoliosis have been developed. These models applied a single load to the spine and could not precisely modulate the spinal growth in different dimensions. In this study, we applied posterior tethering in various directions with the application of nickel-titanium （NT） coil springs in dog＇s spine to modulate spinal growth in the coronal, sagittal, and transverse planes and create a scoliosis model possess curves that mimic adolescent idiopathic scoliosis （AIS） three dimensionally. Methods： Scoliosis was surgically induced in eight 8-week-old female dogs （weight： 1.95–2.30 kg） using bone screws and NT coil springs. The deformity was induced through the placement of posterior NT coil springs that tethered the spine by bone screw fixation. All dogs were monitored with serial radiographs to document changes in deformities. Results： All experimental animals developed scoliotic curves convex to the left in the lumbar segment. The mean coronal Cobb angle was 18.0° immediately postoperatively and 54.5° at 22 weeks. The mean lordosis increased from 6.2° postoperatively to 35.0° at final follow-up. Apical axial rotation increased from 4.5° postoperatively to 31.2° at 22 weeks. Conclusions： With the application of NT springs in dogs that allowed posterior tethering in various directions, lumbar spinal deformity was achieved in three planes： coronal, sagittal, and transverse planes. Notably, the lumbar spine in surgically treated dogs developed lordoscoliosis with obvious rotation and the curves mimic AIS three dimensionally well. This method allows lumbar scoliosis to develop without deep dissection of muscle and maintains the essential anatomical elements along the spinal curve. Moreover, the spinal growth modulation technique could yield information that would provide a basis for developing novel early-stage tr

关键词： Asymmetric Load: Growth Modulation Nonfusion Scoliosis Model

Effect of MoSi_2 content on dielectric and mechanical properties of MoSi_2/Al_2O_3 composite coatings

在线全文

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2012年第1期22卷 111-116页

作者： wu zhi-hong ZHOU Wan-cheng LUO Fa ZHU Dong-mei State Key Laboratory of Solidification Processing Northwestem Polytechnical UniversityXi’an 710072China

Molybdenum disilicide （MoSi2） sheath and aluminum oxide （Al2O3） core blended powders were fabricated by spray drying. A derived coating material was produced for the application as microwave absorbers using the as prepared powders by atmospheric plasma spray （APS） technology. The effects of MoSi2/Al2O3 mass ratio on the dielectric and physical mechanical properties of the composite coatings were investigated. When the MoSi2 content of the composites increases from 0 to 45%, the flexure strength and fracture toughness improve from 198 to 324 MPa and 3.05 to 4.82 MPa-m1/2 then decline to 310 MPa and 4.67 MPa-m1/2, respectively. The dielectric loss tangent increases with increasing MoSi2 content, and the real part of permittivity decreases conversely over the frequency range of 8.2-12.4 GHz. These effects are due to the agglomeration of early molten MoSi2 particles and the increase of the electrical conductivity with increasing MoSi2 content.

关键词： MoSi2/Al2O3 composite coating atmospheric plasma spraying mechanical properties dielectric properties

Plasma levels of receptor interacting protein kinase-3 correlated with coronary artery disease

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2019年第12期132卷 1400-1405页

作者： Xiao-Min Hu Xi Chen Hai-Yu Pang hong-hong Liu Pei-Pei Chen Jing-Lin Shi Si Tang zhi-hong wu Shu-Yang Zhang Medical Science Research Center Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Background: Necroptosis plays an important role in human atherosclerosis and atheroma development. Since receptor interacting protein kinase-3 (RIP3) acts as a key mediator of necroptosis, this study aimed to explore its relationship between plasma RIP3 levels and coronary artery disease (CAD) and discover a potential new biomarker for screening CAD subtypes and severity. Methods: A total of 318 patients with CAD who had coronary angiography and 166 controls in Peking Union Medical College Hospital from September 2017 to January 2018 were enrolled in this study. Patients with CAD were divided into three subgroups: patients with stable coronary artery disease (SCAD), patients with unstable angina (UA), and patients with myocardial infarction (MI). The severity of atherosclerosis was determined by Gensini score (GSS). Logistic regression was used to determine the relationship between plasma RIP3 levels and CAD. The correlation between plasma RIP3 and GSS was calculated using multiple linear regression models. Results: Overall, plasma RIP3 levels were significantly higher than serum RIP3 levels. Plasma RIP3 levels in patients with CAD were significantly higher than those in controls. Plasma RIP3 levels were strongly associated with CAD (odds ratio: 6.00, 95% confidence interval 3.04–11.81;P < 0.001). Plasma RIP3 levels increased linearly from controls to patients with SCAD, then patients with UA, and finally to patients with MI. We found a significantly positive correlation between proportion of cases of acute coronary syndrome in subjects and their plasma RIP3 level quartile. Plasma RIP3 levels were also associated with GSS (B 0.027;standard error 0.012;P < 0.05). Conclusions: Plasma RIP3 levels were independently associated with CAD. Plasma RIP3 levels could potentially supplement clinical assessment to screen CAD and determine CAD severity.

关键词： Receptor interacting protein kinase-3 Coronary artery disease Necroptosis Atherosclerosis Acute coronary syndrome Biomarker

理性论辩的关键能力:预想、构建和回应反论证

在线全文

学校读者我要写书评

暂无评论

湖北大学学报（哲学社会科学版） 2019年第4期46卷 57-66,I0002页

作者：武宏志延安大学政法学院

理性论辩能力是人类进化的成果。论证和反论证的交互作用是理性论辩的基本形式。但是,理性有其阴暗面:人们在论证时往往既偏颇又懒惰,典型表现为“我方偏差”(证实偏差)。克服理性的这种偏差需要对话框架下的反论证发挥作用。反对、反... 详细信息

理性论辩能力是人类进化的成果。论证和反论证的交互作用是理性论辩的基本形式。但是,理性有其阴暗面:人们在论证时往往既偏颇又懒惰,典型表现为“我方偏差”(证实偏差)。克服理性的这种偏差需要对话框架下的反论证发挥作用。反对、反例、反主张(论点、断言)、切断、削弱、反驳、驳倒等关联概念构成反论证的概念网络。论证者应主动设想反论证,重视反论证,遵守保障反论证发挥功能的制度约束,对反论证做出消除性或整合性响应。预想、构建和回应反论证的意识和技能是批判性思维的典型表现。批判性思维培养应该以反论证的学习和践行为突破口,这也是营造健康社会论辩文化的关键一步。

关键词：论证反论证理性认知偏差批判性思维